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Ingestion of Gouda Cheese Ameliorates the Chronic Unpredictable Mild Stress in Mice
Bohyun Yun,Ja Yeon Yoo,Mi Ri Park,Sangdon Ryu,Woong Ji Lee,Hye Jin Choi,Min Kyoung Kang,Younghoon Kim,Sangnam Oh 한국축산식품학회 2020 한국축산식품학회지 Vol.40 No.1
Depression is a kind of mood disorder characterized by decline in motivation, interest, attention, mental activity, and appetite. Although depression is caused by a variety of causes, including genetic, endocrine and environmental stress, mild depression has been reported to improve with diet. Therefore, various type of food sources including functional and nutritional supplement are required to treat the depressive patients. Cheese contains bioactive peptides that have beneficial effects on host health. In particular, Jersey milk has been reported to contain higher solids than does Holstein milk. This study investigated the effects of Gouda cheese from Jersey and Holstein milk on chronic, unpredictable, mildly stressed (CUMS) mice. Here, spontaneous alterations in cheese-fed stressed mice were noted to be effectively recovered with statistical significance regardless cow species. Interestingly, for the analysis of fecal microbiota, Bacteroidetes were noted to increase with a reduction in Firmicutes at the phylum level with Jersey cheese. Taken together, we suggest that cheese intake provided a beneficial effect on stressed mice in recovering recognition ability. In particular, changes in internal microbiota were observed, suggesting that the bioactive ingredients in cheese act as improvement agents with respect to mood and brain function.
Bohyun Yun,Minkyoung Kang,Juyeon Lee,Jiseon Yoo,Sangnam Oh 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
The Caenorhabditis elegans has been widely adopted as an alternative host model for the study of microbial infection. Colostrum contains nutritionally and immunologically important physiologically active ingredients and contains microRNAs that regulate gene expression. The aim of this study is to evaluate whether bovine colostrum-derived exosomes (BCE) enhance the protective response of C. elegans against pathogenic bacteria infection. We observed bovine colostrum-derived exosomes extended the lifespan of worms compared to that of control OP50. In addition, nematode colostrum exosomes promoted resistance of nematodes exposed to four pathogenic bacteria and extended the lifespan of nematodes through killing assay. Using the biological process-related GO enrichment analysis, the significantly upregulated GO terms against C. elegans immunity in BCE-exposed C. elegans included defense response to bacterium, innate immune response, and defense response to gram-positive bacteria. This study clearly showed that BCE have potentiate the host defense of C. elegans increasing life span and suggest as new natural product for antimicrobial activity.
Bovine Colostrum-derived Exosomes Improve Muscle Atrophy
Bohyun Yun,Minkyoung Kang,Juyeon Lee,Jiseon Yoo,Sangnam Oh 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
Muscle atrophy is caused by mainly associated with aging and degenerative disease, characterized by systemic chronic inflammation, and significantly decreases the quality of life. Recent researches showed milk exosomes contains abundant microRNAs and have biological impacts related to immunity, inflammation, osteoporosis, and gut microbiota. Here, we focused on evaluating the noble functionality of bovine colostrum-derived exosomes (BCE) for improving muscle atrophy. Interestingly, the C2C12 mice muscle cells exposed to dexamethasone (DEX) for 24 h significantly showed decreased myotube diameter, which were all increased by treatment with 20 to 200 μg/mL BCE. Furthermore, treatment of BCE for DEX-induced C2C12 myotubes reduced the expression of MuRF1a, a ubiquitin E3 ligase marker. The expression of muscle differentiation markers and myosin heavy chains of atrophy-induced cells was recovered by the treatment of BCE. Next, RT-qPCR analysis of C. elegans muscle-related genes showed that BCE upregulated muscle-related genes in worms. Collectively, this study suggests that BCE provided the beneficial activity in vitro and in vivo for preventing from the sarcopenia.
( Bohyun Yun ),( Hyun-mi An ),( Won-bo Shim ),( Won-il Kim ),( Nguyen Bao Hung ),( Sanghyun Han ),( Hyun-ju Kim ),( Seungdon Lee ),( Se-ri Kim ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 Journal of microbiology and biotechnology Vol.27 No.12
This study was conducted to develop a screening method using Colilert-18 and a device for the detection of E. coli from agri-food production environments and fresh vegetables. The specificity and sensitivity of Colilert-18 by temperature (37°C and 44°C) were evaluated with 38 E. coli and 78 non-E. coli strains. The false-positive rate was 3.8% (3/78) and 0% (0/78) at 37°C and 44°C, respectively. The detection limit of E. coli at 37°C at <1.0 log CFU/250 ml was lower than that at 44°C. The efficiency of the developed device, which comprised an incubator equipped with a UV lamp to detect E. coli in the field, was evaluated by measuring the temperature and UV lamp brightness. The difference between the set temperature and actual temperature of the developed device was about 1.0°C. When applying the developed method and device to various samples, including utensils, gloves, irrigation water, seeds, and vegetables, there were no differences in detection rates of E. coli compared with the Korean Food Code method. For sanitary disposal of culture samples after experiments, the sterilization effect of sodium dichloroisocyanurate (NaDCC) tablets was assessed for use as a substitute for an autoclave. The addition of one tablet of NaDCC per 50 ml was sufficient to kill E. coli cultured in Colilert-18. These results show that the developed protocol and device can efficiently detect E. coli from agri-food production environments and vegetables.