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RISS 인기검색어
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- 저자 : Yuanfan Xiao (검색결과 2 건)
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Yi Zhong,Ai Jiang,Fei Sun,Yuanfan Xiao,Ying Gu,Lei Wu,Yujie Zhang,Hongcan Shi 한국조직공학과 재생의학회 2019 조직공학과 재생의학 Vol.16 No.1
BACKGROUND: Different decellularization methods can affect the integrity and the biomechanical and biocompatible properties of the tracheal matrix. Natural cross-linking with genipin can be applied to improve those properties. The goals of this study were to evaluate the effects of different decellularization methods on the properties of genipin-cross-linked decellularized tracheal matrices in rabbits. METHODS: The tracheas of New Zealand rabbits were decellularized by the Triton-X 100-processed method (TPM) and the detergent-enzymatic method (DEM) and were then cross-linked with genipin. Mechanical tests, haematoxylin–eosin staining, Masson trichrome staining, Safranin O staining, DAPI staining, scanning electronic microscopy (SEM), and biocompatibility tests were used to evaluate the treatment. The bioengineered trachea and control trachea were then implanted into allogeneic rabbits for 30 days. The structural and functional analyses were performed after transplantation. RESULTS: The biomechanical tests demonstrated that the biomechanical properties of the decellularized tracheas decreased and that genipin improved them (p\0.05). The histological staining results revealed that most of the mucosal epithelial cells were removed and that the decellularized trachea had lower immunogenicity than the control group. The analysis of SEM revealed that the decellularized trachea retained the micro- and ultra-structural architectures of the trachea and that the matrices cross-linked with genipin were denser. The biocompatibility evaluation and in vivo implantation experiments showed that the decellularized trachea treated with the DEM had better biocompatibility than that treated with the TPM and that immunogenicity in the cross-linked tissues was lower than that in the uncross-linked tissues (p\0.05). CONCLUSIONS: Compared with the trachea treated with the TPM, the rabbit trachea processed by the DEM had better biocompatibility and lower immunogenicity, and its structural and mechanical characteristics were effectively improved after the genipin treatment, which is suitable for engineering replacement tracheal tissue.
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Development of Parallel Plate Avalanche Counter for heavy ion collision in radioactive ion beam
Wei, Xianglun,Guan, Fenhai,Yang, Herun,Wang, Yijie,Zhang, Junwei,Ma, Peng,Diao, Xinyue,Lu, Chengui,Li, Meng,Guan, Yuanfan,Duan, Limin,Hu, Rongjiang,Zhang, Xiuling,Xiao, Zhigang Korean Nuclear Society 2020 Nuclear Engineering and Technology Vol.52 No.3
We have developed a position-sensitive Parallel Plate Avalanche Counter (PPAC) to detect the fission fragments and reconstruct the fission reaction plane in the experiment of studying nuclear equation of state (nEOS) by means of heavy ion collision (HIC). This experiment put forward high requirements for the performances of PPAC, such as the time resolution, efficiency and position resolution. According to these requirements we designed the PPAC with an active area of 240 mm × 280 mm working at low gas pressure. The results show that time resolution could be less than 300 ps. Position resolution is consistent with the theoretical calculation about 1.35 mm. Detection efficiency could be approaching 100% gradually with the voltage increasing in different gas pressures. The performances of PPAC have also been verified in beam experiment. Each set of anode wires can be accurately separated in the position spectrum. In the beam experiment, we also got the back-to-back correlation of fission fragments which is one of the direct signals characterizing binary decay.
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