Ce4+-Stimulated Ion Fluxes Are Responsible forApoptosis and Taxol Biosynthesis in Suspension Cultures of Taxus Cells

Ying-Jin Yuan,Jing-Chuan Li,Zhi-Qiang Ge 한국생물공학회 2005 Biotechnology and Bioprocess Engineering Vol.10 No.2

Ion fluxes across the plasma membrane activated by 1 mM Ce4+, cell apoptosis and taxol biosynthesis in suspension cultures of Taxus cuspidata were studied. The extracellular pH sharply decreased upon the addition of 1 mM Ce4+, then increased gradually and exceeded the initial pH value over a time period of 12 h. The extracellular Ca2+ concentration decreased within the first 3 h after the addition of Ce4+, then gradually decreased to one third of initial value in control at about 72 h and remained unchanged afterwards. Experiments with an ion channel blocker and a Ca2+-channel blocker indicated that the dynamic changes in extracellular pH and the Ca2+ concentration resulted from the Ce4+-induced activation of H+ uptake and Ca2+ influx across the plasma membrane via ion channels. A pretreatment of the ion channel blocker initiated Ce4+-treated cells to undergo necrosis, and the prior addition of the Ca2+-channel blocker inhibited Ce4+-induced taxol biosynthesis and apoptosis. It is thus inferred that H+ uptake is necessary for cells to survive a Ce4+-caused acidic environment and is one of the mechanisms of Ce4+-induced apoptosis. Furthermore, the Ca2+ influx across the plasma membrane mediated both the Ce4+-induced apoptosis and taxol biosynthesis.

Germline Mutations of hMLH1 and hMSH2 Genes in Korean Hereditary Nonpolyposis Colorectal Cancer

Yuan Ying,Nakamura,Yusuke,Kim,Jin-Pok,Won,Yong-Jin,Choe,Kuk Jin,Lee,Kee Hyung,Kim,Cheong Yong,Kang,Koo Jeong,Oh,Jae-Hwan,Han,Hye-Jung,Kim,Sung,Park,Jae-Gahb,Ku,Ja-Lok,Kim,Kwang Yun,Oh,Nahm-Gun 가톨릭중앙의료원 가톨릭암센터 1996 암심포지움 Vol.- No.2

Hereditary nonpolyposis colorectal cancer(HNPCC) is one of the most common autosomal donimamtly inherited diseases, affecting as many as one in every 200-400 individuals in the Western world. HNPCC is responsible for up to 15% of all colorectal cancers (1). It has recently been shown that the mismatch repair genes, hMSH2, hMLH1, hPMS1, and hPMS2, are mutated in the germline of affected members in HNPCC families(2-6), as well as for some apparently nonhereditary cancer patients(7-9). A combination of linkage and mutational aanalysis has indicated that hMSH2 and hMLH1 are likely to be the prevalent genes responsible for HNPCc, accounting for 80%~90% of HNPCC cases(10-13), while hPMS1 and hPMS2 are thought to account for only a minor fraction of cases. The indentification of the causative mutations in HNPCC families is desirable, since it allows the carrier status of unaffected relatives at risk to be determined.

rs12904 Polymorphism in the 3'UTR of EFNA1 is Associated with Colorectal Cancer Susceptibility in a Chinese Population

Mao, Ying-Ying,Jing, Fang-Yuan,Jin, Ming-Juan,Li, Ying-Jun,Ding, Ye,Guo, Jing,Wang, Fen-Juan,Jiang, Long-Fang,Chen, Kun Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.9

Accumulated evidence has indicated that Ephrin A1 (EFNA1) is associated with angiogenesis and tumorigenesis in various types of malignancies, including colorectal cancer (CRC). In the current study, we performed an online search using the public microarray database to investigate whether EFNA1 expression might be altered in CRC tissues. We then conducted a case-control study including 306 subjects (102 cases and 204 well-matched controls) in Xiaoshan County to assess any association between genetic polymorphisms in EFNA1 and CRC susceptibility. Searches in the Oncomine expression profiling database revealed EFNA1 to be overexpressed in CRC tissue compared with adjacent normal tissue. The rs12904 G-A variant located in the 3' untranslated region (UTR) of EFNA1 was observed to be associated with CRC susceptibility. Compared with the AA homozygous genotype, those carrying GA genotype had a decreased risk of developing CRC (odds ratio (OR)=0.469, 95% confidence interval (CI): 0.225-0.977, and P=0.043). The association was stronger among smokers and tea drinkers, however, no statistical evidence of interaction between rs12904 polymorphism and smoking or tea drinking on CRC risk was found. Our results suggest that EFNA1 is involved in colorectal tumorigenesis, and rs12904 A>G polymorphism in the 3' UTR of EFNA1 is associated with CRC susceptibility. Larger studies and further mechanistic investigations are warranted to confirm our findings.

Identification of peroxiredoxin II and its related molecules as potential biomarkers of dermal mesenchymal stem cell homing using network analysis

Han Ying-Hao,Mao Ying-Ying,Feng Yao-Yuan,Xiang Hong-Yi,Sun Hu-Nan,Jin Mei-Hua,Kwon Taeho 한국응용생명화학회 2022 Applied Biological Chemistry (Appl Biol Chem) Vol.65 No.3

In this study, we performed RNA sequencing of Prx II +/+ and Prx II −/− dermal mesenchymal stem cells (DMSCs) to identify differentially expressed genes (DEGs). To explore the role of Prx II in DMSCs, we performed Gene Ontology analysis of the DEGs. The results showed that the DEGs were mainly involved in the biological processes of cell migration, intercellular adhesion, and coordination of the regulation of stem cell homing. Through the construction of protein–protein interaction network, four hub genes Cd274 , Ccl5 , Il1b , and Stat1 involved in cell adhesion and cell homing were screened. Quantitative reverse transcription PCR analysis showed that Cd274 , Ccl5 , Il1b , and Stat1 were down regulated in Prx II −/− DMSCs. miRwalk and Starbase databases were further used to screen the upstream molecules miRNA and lncRNA regulating hub gene. Prx II was found to be involved in the regulation of stem cell homing via the Tctn2/miR-351/Stat1/Il1b axis. Thus, we demonstrated that Prx II is a key molecule in the regulation of the homing ability of DMSCs. Our results provide a theoretical foundation for improving the homing ability of DMSCs by targeting Prx II.

A Genetic Variant in MiR-146a Modifies Digestive System Cancer Risk: a Meta-analysis

Li, Ying-Jun,Zhang, Zhen-Yu,Mao, Ying-Ying,Jin, Ming-Juan,Jing, Fang-Yuan,Ye, Zhen-Hua,Chen, Kun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.1

MicroRNAs (miRNAs) negatively regulate gene expression and act as tumor suppressors or oncogenes in oncogenesis. The association between a single nucleotide polymorphism (SNP) in miR-146a rs2910164 and susceptibility to digestive system cancers was inconsistent in previous studies. In this study, we conducted a literature search of PubMed to identify all relevant studies published before August 31, 2013. A total of 21 independent case-control studies were included in this updated meta-analysis with 9,558 cases and 10,614 controls. We found that the miR-146a rs2910164 polymorphism was significantly associated with decreased risk of digestive system cancers in an allele model (OR=0.90, 95%CI 0.87-0.94), homozygote model (OR=0.84, 95%CI 0.77-0.91), dominant model (OR=0.90, 95%CI 0.84-0.96), and recessive model (OR=0.85, 95%CI 0.79-0.91), while in a heterozygous model (OR = 0.99, 95% CI 0.89-1.11) the association showed marginal significance. Subgroup analysis by cancer site revealed decreased risk in colorectal cancer above allele model (OR=0.90, 95%CI 0.83-0.97) and homozygote model (OR=0.85, 95%CI 0.72-1.00). Similarly, decreased cancer risk was observed when compared with allele model (OR=0.87, 95%CI 0.81-0.93) and recessive model (OR=0.81, 95%CI 0.72-0.90) in gastric cancer. When stratified by ethnicity, genotyping methods and quality score, decreased cancer risks were also observed. This current meta-analysis indicated that miR-146a rs2910164 polymorphism may decrease the susceptibility to digestive system cancers, especially in Asian populations.

Health management of laser rangefinder considering maximum continuous working time

Ying Zeng,Hai-Tao Chen,Yu-Jin Wei,Yuan Chen,Hong-Zhong Huang 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.10

High-power lasers have severe thermal effects. The heat buildup during longterm operation will increase the threshold current of the laser and the internal defects, which will seriously affect the lifetime of the device. A temperature sensor is difficult to install on the laser rangefinder due to the small size of the solid-state laser device. Considering this situation, this work analyses the thermal mechanism of the laser rangefinder and proposes a temperature calculation method for the crystal working substance inside the laser rangefinder based on the finite element simulation to obtain the crystal center temperature and thermal model with the working time. Then, the prediction model of the maximum continuous working time of the laser can be obtained. The proposed model can provide an early scientific warning for the remaining continuous working time of the solid-state laser and improve the single-shot utilization of the solid-state laser to a certain extent. A comprehensive health management framework of laser rangefinders including FMEA is also proposed.

Molecular Analysis and Expression Patterns of the 14-3-3 Gene Family from Oryza Sativa

( Yuan Yao ),( Ying Du ),( Lin Jiang ),( Jin Yuan Liu ) 생화학분자생물학회 2007 BMB Reports Vol.40 No.3

Engineering the morphology <i>via</i> processing additives in multiple all-polymer solar cells for improved performance

Yuan, Jianyu,Xu, Yalong,Shi, Guozheng,Ling, Xufeng,Ying, Lei,Huang, Fei,Lee, Tack Ho,Woo, Han Young,Kim, Jin Young,Cao, Yong,Ma, Wanli The Royal Society of Chemistry 2018 Journal of materials chemistry. A, Materials for e Vol.6 No.22

<P>In this contribution, we report the working mechanisms of several processing additives for controlling the morphology of four all-polymer systems. The optical and electrical properties, photovoltaic performance, morphology and the dynamic process of film formation of these all-polymer systems were thoroughly examined. We revealed that the effect of additives is largely dependent on the aggregation behaviors of the polymers used. Here, the polymer acceptors with large planar structures have stronger inter-chain interactions, which make their morphology more susceptible to additive treatment compared to the donors. 1,8-Di(R)octane (R = Cl, Br, and I) additives can be applied to multiple all-polymer devices with improved efficiency due to their general capability to increase the crystallinity and extend the effective time during the film formation. Interestingly, DBrO outperforms the widely used DIO, obtaining a highest efficiency of 8.23% for the PTzBI/P(NDI2OD-T2) based all-polymer solar cells, indicating finer morphology control by a subtle change of the additive structure. In contrast, the addition of chloronaphthalene (CN) can alleviate the inter-chain interaction of polymers to prevent the formation of oversized domains, which make it especially efficient for systems using strongly aggregated polymers like P(NDI2OD-T2). Our results provide insight into processing additives and suggest guidelines to rationally select additives for nonfullerene solar cells.</P>

Edaphovirga cremea gen. nov., sp. nov., isolated from the rhizospheric soil of Codonopsis clematidea

Jin-Yan Xue,Meng-Yue Zhang,Yu Zhang,Juan Cheng,Li-Cheng Liu,Ying-Ying Wu,Tian-Yuan Zhang,Yi-xuan Zhang 한국미생물학회 2019 The journal of microbiology Vol.57 No.5

A Gram-negative, facultatively anaerobic, non-motile, nonspore- forming, coccoid or rod-shaped and creamy-pigmented bacterium, designated SYP-B2100T, was isolated from the rhizospheric soil of Codonopsis clematidea in the Xinjiang Uygur Autonomous Region, China. The optimal growth occurred at 28°C, pH 5.0, in the absence of NaCl. The cells tested positive in catalase and methyl red tests but negative in oxidase, urease, gelatinase, milk coagulation, and peptonisation, H2S production, nitrate reduction, and Voges-Proskauer tests. The major isoprenoid quinone was ubiquinone-8 (Q-8). The major cellular fatty acids were C16:0 and summed feature 8. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol. The 16S rRNA gene sequence of strain SYP-B2100T was the most similar to that of Rahnella inusitata DSM 30078T (96.9%) within the family Enterobacteriaceae. The genomic DNA G+C content of strain SYP-B2100T was 50.3 mol%. The combined data from the phylogenetic, morphological, physiological, biochemical, and chemotaxonomic analyses presented in this study support the conclusion that strain SYP-B2100T represents a novel species of a new genus, for which the name Edaphovirga cremea gen. nov., sp. nov. is proposed; the type strain is SYPB2100T (= CGMCC 1.5857T = DSM 105170T = KCTC 62024T).

Molecular Analysis and Expression Patterns of the 14-3-3 Gene Family from Oryza Sativa

Yao, Yuan,Du, Ying,Jiang, Lin,Liu, Jin-Yuan Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.3

The ubiquitous family of 14-3-3 proteins functions as regulators in a variety of physiological processes. Eight rice 14-3-3 genes, designated OsGF14a through h, were identified from an exhaustive search of the genome database. Comparisons of deduced amino acid sequences reveal a high degree of identity among members of the OsGF14 family and reported Arabidopsis 14-3-3 proteins. A phylogenetic study indicates that OsGF14s contain both $\varepsilon$ and non-$\varepsilon$ forms, which is also confirmed by a structural analysis of OsGF14 genes. Furthermore, transcripts of OsGF14b, OsGF14c, OsGF14d, OsGF14e, OsGF14f and OsGF14g were detected in rice tissues. Their different expression patterns, the different effects of environmental stresses and plant hormones on their transcription levels, and the different complementary phenotypes in yeast 14-3-3 mutants not only indicates that OsGF14s are responsive to various stress conditions and regulated by multiple signaling pathways, but also suggests that functional similarity and diversity coexist among the members of OsGF14 family.