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( Yu Ji Lee ),( Na Ree Kang ),( Jung Eun Lee ),( Woo Seong Huh ),( Sung Joo Kim ),( Yoon Goo Kim ),( Dae Joong Kim ),( Ha Young Oh ) 대한신장학회 2009 Kidney Research and Clinical Practice Vol.28 No.4
Purpose: The objective of this study was to investigate the clinical manifestations and risk factors associated with acute graft pyelonephritis (AGPN) and its impact on graft outcome. Methods: We performed a retrospective study reviewing the medical records of 272 recipients with a graft survival of more than 1 year among 291 recipients that had undergone kidney transplantation between January 1995 and December 1999. Results: Twenty eight (10.3%) patients had at least one episode of AGPN during a follow-up of 7.3 years, and 14 (50%) among them had recurrent episodes. 31.5% of total AGPN episodes had no any urinary signs and symptoms and only had a fever and leukocyturia. Escherichia coli and Klebsiella, the most common pathogens isolated, were susceptible to ciprofloxacin in 48.6% of cases and to ceftriaxone in 94.3% of cases. Acute rejection was not associated with the occurrence of AGPN. However, female gender was a risk factor for acute rejection (risk ratio 7.11, p<0.001). Vesicoureteral reflux in allograft was found in 72.7% of the recipients with an episode of AGPN (16/22). There was a trend toward more frequent development of vesicoureteral reflux in recipients with recurrent AGPN episodes (54.6% in patients with a single episode vs 90.9% in patients with recurrent episodes, p=0.074). On Cox regression/Time-dependent covariate analysis, AGPN had no significant association with the graft or patient survival. Conclusion: AGPN is common after kidney transplantation, especially in women. However, AGPN was not associated with a poor long-term graft outcome.
Clinical Characteristics of Cholinergic Urticaria in Korea
( Jung Eun Kim ),( Young Sun Eun ),( Young Min Park ),( Hyun Jeong Park ),( Dong Su Yu ),( Hoon Kang,),( Sang Hyun Cho ),( Chul Jong Park ),( Si Yong Kim ),( Jun Young Lee ) 대한피부과학회 2014 Annals of Dermatology Vol.26 No.2
Background: Cholinergic urticaria is a type of physical urticaria characterized by heat-associated wheals. Several reports are available about cholinergic urticaria; however, the clinical manifestations and pathogenesis are incompletely understood. Objective: The purpose of this study was to investigate the clinical characteristics of cholinergic urticaria in Korea. Methods: We performed a retrospective study of 92 patients with cholinergic urticaria who were contacted by phone and whose diagnoses were confirmed by the exercise provocation test among those who had visited The Catholic University of Korea, Catholic Medical Center from January 2001 to November 2010. Results: All 92 patients were male, and their average age was 27.8 years (range, 17∼51 years). Most of the patients had onset of the disease in their 20s and 30s. Non-follicular wheals were located on the trunk and upper extremities of many patients, and the symptoms were aggravated by exercise. Eight patients showed general urticaria symptoms and 15 had accompanying atopic disease. Forty-three patients complained of seasonal aggravation. Most patients were treated with first and second- generation antihistamines. Conclusion: Dermatologists should consider these characteristics in patients with cholinergic urticaria. Further investigation and follow-up studies are necessary to better understand the epidemiological and clinical findings of cholinergic urticaria.
Yu Kyung Park(박유경),Chang-Eun Lee(이창은),Hyoungseok Lee(이형석),Hye Yeon Koh(고혜연),Sojin Kim(김소진),Sung Gu Lee(이성구),Jung Eun Kim(김정은),Joung Han Yim(임정한),Ju-Mi Hong(홍주미),Ryeo-Ok Kim(김려옥),Se Jong Han(한세종),Il-Ch 한국생명과학회 2020 생명과학회지 Vol.30 No.11
TCTP는 다양한 진핵생물에서 풍부하게 존재하는 단백질 중에 하나이며, 암, 세포 증식, 유전자 조절 등과 관련된 세포의 생리학적 기작에서 중요한 역할을 담당하는 것으로 알려져 왔다. 더구나, TCTP는 dithiothreitol (DTT)나 hydrogen peroxide (H₂O₂)에 의해 유도되는 산화적 스트레스에 대한 저항성에 관여하는 중요한 단백질로 주목받아 왔다. 한편, 극지역 서식 생물들은 강한 자외선에 의해 발생한 활성산소를 조절하기 위한 다양한 항산화 방어체계를 가지고 있다. 본 연구에서는 북극 동물플랑크톤 Calanus glacialis에서 분리된 TCTP가 산화적 스트레스 하에서 E. coli 세포의 저항성에 미치는 효과를 관찰하였다. C. glacialis에서 분리된 TCTP 유전자(ORF 522 bp) 서열을 분석하였고, 약 23 kDa의 재조합 단백질을 제작하였다. 관찰 결과, TCTP 재조합 단백질이 E. coli 세포에서 과발현되었을 때, 세포들은 H₂O₂에 의해 유도된 산화적 스트레스에 대한 저항성이 증가하는 것을 확인하였다. 본 관찰을 통해, 북극 C. glacialis TCTP 단백질의 항산화 조절자로서의 역할에 대한 가능성을 처음으로 제시하였다. Translationally controlled tumor protein (TCTP) is one of the most abundant proteins in various eukaryotic organisms. TCTPs play important roles in cell physiological processes in cancer, cell proliferation, gene regulation, and heat shock response. TCTP is also considered an important factor in the resistance to oxidative stress induced by dithiothreitol or hydrogen peroxide (H₂O₂). Arctic calanoid copepods have a variety of antioxidant defense systems to regulate the levels of potentially harmful reactive oxygen species generated by ultraviolet radiation in the Arctic marine ecosystem. However, information on the antioxidant activity of TCTP in the Arctic Calanus glacialis is still scarce. To understand the putative antioxidant function of the Arctic copepod C. glacialis TCTP (Cg-TCTP), its gene was cloned and sequenced. The Cg-TCTP comprised 522 bp and encoded a 174-amino acid putative protein with a calculated molecular weight of ~23 kDa. The recombinant Cg-TCTP (Cg-rTCTP) gene was overexpressed in Escherichia coli (BL21), and Cg-rTCTP-transformed cells were grown in the presence or absence of H₂O₂. Cg-rTCTP-transformed E. coli showed increased tolerance to high H₂O₂ concentrations. Therefore, TCTP may be an important antioxidant protein related to tolerance of the Arctic copepod C. glacialis to oxidative stress in the harsh environment of the Arctic Ocean.
Detergent Free Fractionation of Nuclei from Mouse Embryonic Stem Cells
( Yu Ri Kim ),( Eun Kyung Song ),( Jung Min Yim ),( Chae Hwa Yoo ),( Sang Jae Rhee ),( Hyung Tae Kim ),( Byung Soo Kim ),( Myung Kwan Han ) 한국조직공학·재생의학회 2011 조직공학과 재생의학 Vol.8 No.2
Mouse embryonic stem cells (ESCs), with their ability to self-renewal, and to differentiate into virtually all types, have become the major focus in contemporary cell-based therapy and regenerative medicine. Many studies about the signaling mechanism regulating self-renewal or differentiation have been performed in mouse ESCs. Pure nuclei fractionation is needed for the studies of gene regulation involved in self-renewal or differentiation of ESCs. Here we develop more efficient method for isolation of pure nuclei from mouse ESCs than the conventional method. We isolated more pure nuclei fraction with detergent-free buffer by freezing-thawing cycling. Mouse ESC nuclei isolated by our method were more pure than those by the conventional method. Oct4, a well-known stemness marker and nucleus protein, in the nuclei fraction, specifically bound to the oct4 binding site. Light electroscopy and electron microscopy showed the intactness of the isolated nuclei fraction. Flow cytometric analysis of the population of isolated nuclei revealed no signs of cell cycle specific losses of nuclei during isolation procedure. This contributes to studying nuclear signaling molecules involved in self-renewal and differentiation in mouse ESCs.
Rapid <i>In Vitro</i> Corrosion Induced by Crack-Like Pathway in Biodegradable Mg-10% Ca Alloy
Jung, Jae-Young,Kwon, Sang-Jun,Han, Hyung-Seop,Yang, Gui Fu,Lee, Ji-Young,Yang, Seok-Jo,Cho, Sung-Youn,Cha, Pil-Ryung,Kim, Young-Yul,Kim, Yu-Chan,Seok, Hyun-Kwang,Ahn, Jae-Pyoung Cambridge University Press 2013 Microscopy and Microanalysis Vol.19 No.5
<B>Abstract</B><P>The <I>in vitro</I> corrosion mechanism of the biodegradable cast Mg-10% Ca binary alloy in Hanks' solution was evaluated through transmission electron microscopy observations. The corrosion behavior depends strongly on the microstructural peculiarity of Mg2Ca phase surrounding the island-like primary Mg phase and the fast corrosion induced by the interdiffusion of O and Ca via the Mg2Ca phase of lamellar structure. At the corrosion front, we found that a nanosized crack-like pathway was formed along the interface between the Mg2Ca phase and the primary Mg phase. Through the crack-like pathway, O and Ca are atomically exchanged each other and then the corroded Mg2Ca phase was transformed to Mg oxides. The <I>in vitro</I> corrosion by the exchange of Ca and O at the nanosized pathway led to the rapid bulk corrosion in the Mg-Ca alloys.</P>
Jung, Hyung-Suk,Yu, Il-Hyuk,Kim, Hyo Kyeom,Lee, Sang Young,Lee, Joohwi,Choi, Yujin,Chung, Yoon Jang,Lee, Nae-In,Park, Tae Joo,Choi, Jung-Hae,Hwang, Cheol Seong IEEE 2012 IEEE transactions on electron devices Vol.59 No.9
<P>The dielectric performance and charge trapping properties of <TEX>$ \hbox{HfO}_{2}$</TEX> on a Ge substrate with various passivating interfacial layers (PILs), such as <TEX>$\hbox{SiO}_{x}\hbox{N}_{y}$</TEX>, <TEX> $\hbox{AlO}_{x}\hbox{N}_{y}$</TEX>, <TEX>$\hbox{HfO}_{x}\hbox{N}_{y}$</TEX> , and <TEX>$\hbox{LaO}_{x}\hbox{N}_{y}$</TEX>, are investigated. The large capacitance–voltage (<TEX>$C$</TEX>– <TEX>$V$</TEX>) hysteresis of <TEX>$ \hbox{HfO}_{2}$</TEX> on a Ge substrate (<TEX>$\sim$</TEX>1500 mV) was not improved by inserting either <TEX>$\hbox{HfO}_{x}\hbox{N}_{y}$</TEX> or <TEX>$\hbox{LaO}_{x}\hbox{N}_{y}$</TEX> PIL between the <TEX>$\hbox{HfO}_{2}$</TEX> and Ge substrates, while both <TEX>$\hbox{SiO}_{x}\hbox{N}_{y}$</TEX> and <TEX>$\hbox{AlO}_{x}\hbox{N}_{y}$</TEX> PILs induced a noticeable reduction of <TEX>$C$</TEX>–<TEX>$V$</TEX> hysteresis. As the PILs' thicknesses increased, the <TEX>$C$</TEX>– <TEX>$V$</TEX> hysteresis of <TEX>$ \hbox{HfO}_{2}$</TEX> with <TEX>$\hbox{SiO}_{x}\hbox{N}_{y}$</TEX> PIL decreased to almost zero, while that of <TEX>$\hbox{HfO}_{2}$</TEX> with <TEX>$\hbox{AlO}_{x}\hbox{N}_{y}$</TEX> PIL decreased but was saturated at approximately 400 mV. Furthermore, the charge trapping property of <TEX>$ \hbox{HfO}_{2}$</TEX> with <TEX>$\hbox{SiO}_{x}\hbox{N}_{y}$</TEX> PIL on a Ge substrate is comparable to that of <TEX>$\hbox{HfO}_{2}$</TEX> grown on a Si substrate. Negligible <TEX>$C$</TEX>– <TEX>$V$</TEX> hysteresis and negligible charge trapping of <TEX>$\hbox{HfO}_{2}$</TEX> with <TEX>$ \hbox{SiO}_{x}\hbox{N}_{y}$</TEX> PIL were understood from the fact that <TEX>$\hbox{SiO}_{x}\hbox{N}_{y}$</TEX> is more resistant to react with a Ge substrate and defective Ge suboxides are efficiently suppressed during the formation of <TEX>$\hbox{SiO}_{x} \hbox{N}_{y}$</TEX> PIL and <TEX>$\hbox{HfO}_{2}$</TEX> layers.</P>