Mechanisms of Electroacupuncture Pretreatment in Alleviating Myocardial Ischemia Reperfusion Injury: Interactions between the Cerebellar Fastigial Nucleus and Lateral Hypothalamic Area

Yu Qing,Wu Li-bin,Zhang Fan,Wei Xiao-tong,Chen Pian-pian,Wang Shuai-ya,Cai Mei-yi,Shu Qi,Li Liao-yuan,Wu Zi-jian,Cai Rong-lin,Hu Ling 사단법인약침학회 2021 Journal of Acupuncture & Meridian Studies Vol.14 No.6

Background: Myocardial ischemia reperfusion injury (MIRI) is an important mechanism of post-myocardial infarction injury and a main cause of death in patients with ischemic heart disease. Electroacupuncture (EA) pretreatment is effective for the prevention and treatment of MIRI, but mechanisms mediating the effects of cardiovascular disease EA treatments remain unclear. Objectives: To determine whether the lateral hypothalamus (LHA) and the cerebellar fastigial nucleus (FN) are involved in the protective effects of EA stimulation on MIRI. Methods: EA pretreatment was performed for 7 days before the establishment of the MIRI model. ST-segment changes on electrocardiograms were recorded and the Curtis–Walker arrhythmia score was used to evaluate changes in reperfusion injury. Hematoxylin–eosin staining was applied to evaluate the pathological and morphological changes in myocardial tissue. c-fos expression in the LHA and FN was determined by immunofluorescence staining. Glutamic (Glu) and γ-Aminobutyric acid (GABA) levels were measured using a high-performance liquid chromatography-electrochemical method. Results: EA pretreatment reduced ST-segment elevation, arrhythmia scores, and morphological changes in MIRI myocardial cells in rats, and decreased the c-fos protein expression in LHA/FN nuclei. MIRI was associated with an imbalance between GABA and Glu levels, whereas EA pretreatment increased GABA levels and decreased Glu levels in the LHA/FN. Conclusion: FN and LHA are involved in the EA-mediated attenuation of MIRI. Pretreatment with EA plays a protective role in the myocardium by regulating Glu and GABA release in the LHA and FN.

Occipital, parietal, and frontal cortices selectively maintain task-relevant features of multi-feature objects in visual working memory

Yu, Qing,Shim, Won Mok Elsevier 2017 NeuroImage Vol.157 No.-

<P><B>Abstract</B></P> <P>Previous studies have shown that information held in visual working memory is represented in the occipital, parietal, and frontal cortices. However, less is known about whether the mnemonic information of multi-feature objects is modulated by task demand in the parietal and frontal regions. To address this question, we asked participants to remember either color or orientation of one of the two colored gratings for a delay. Using fMRI and an inverted encoding model, we reconstructed population-level, feature-selective responses in the occipital, parietal and frontal cortices during memory maintenance. We found that not only orientation but also color information can be maintained in higher-order parietal and frontal cortices as well as the early visual cortex when it was cued to be remembered. Conversely, neither the task-irrelevant feature of the cued object, nor any feature of the uncued object was maintained in the occipital, parietal, or frontal cortices. These results suggest a highly selective mechanism of visual working memory that maintains task-relevant features only.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Non-spatial features can be maintained in the parietal and frontal cortices. </LI> <LI> Feature-specific representations in visual working memory are highly selective. </LI> <LI> Only task-relevant features are maintained during visual working memory. </LI> </UL> </P>

Study on the Flavonoids of Lespedeza hedysaroides

Yu-qing Wang,Alamus,Qi-zhong Sun,Hong-xin Wu 한국초지조사료학회 2009 한국초지조사료학회 학술대회논문집 Vol.2009 No.08

Chemical investigation of the aerial parts of Lespedaza hedysaroides resulted in the isolation of 8 flavonoids:orientin (1), isoorientin (2), vitexin (3), isovitexin (4), Isomyricitrin (5), meletin-3-O-β-d-glucoside (6), luteolin-7-O-glucoside (7), 6-Xylopyranosylluteolin (8), The result showed t㏊t Lespedeza hedysaroides was rich in flavonoid glycosides, espically of C-glycosylflavones which have good Pharmacia and bioactive. So Lespedeza hedysaroides was concluded that it has vast prospect for exploitation and utilization.

Comparison of Hybridization Behavior between Double and Single Strand of Targets and the Application of Asymmetric PCR Targets in cDNA Microarray

( Qing Wei ),( San Zhen Liu ),( Jian Feng Huang ),( Xue Ying Mao ),( Xiao Hui Chu ),( Yu Wang ),( Min Yan Qiu ),( Yu Min Mao ),( Yi Xie ),( Yao Li ) 생화학분자생물학회 2004 BMB Reports Vol.37 No.4

Double stranded targets on the cDNA microarray contain representatives of both the coding and noncoding strands, which will introduce hybridization competition with probes. Here, the effect of double and single strands of targets on the signal intensity and the ratios of Cy5/Cy3 within the same slide were compared. The results show that single stranded targets can increase the hybridization efficiency without changing the Cy5/Cy3 ratio. Based on these results, a new strategy was established by generating cDNA targets with asymmetric PCR, instead of conventional PCR, to increase the sensitivity of the cDNA microarray. Furthermore, the feasibility of this approach was validated. The results indicate that the cDNA microarray system based on asymmetric PCR is more sensitive, with no decrease in the reliability and reproducibility as compared with that based on conventional symmetric PCR.

Proteomics and its Application in the Research of Liver Diseases

( Qing Yu He ) 대한소화기학회 2005 SIDDS Vol.8 No.-

TAZ as a novel regulator of oxidative damage in decidualization via Nrf2/ARE/Foxo1 pathway

Yu Hai-Fan,Zheng Lian-Wen,Yang Zhan-Qing,Wang Yu-Si,Wang Ting-Ting,Yue Zhan-Peng,Guo Bin 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

TAZ, as a crucial effector of Hippo pathway, is required for spermatogenesis and fertilization, but little is known regarding its physiological function in uterine decidualization. In this study, we showed that TAZ was localized in the decidua, where it promoted stromal cell proliferation followed by accelerated G1/S phase transition via Ccnd3 and Cdk4 and induced the expression or activity of stromal differentiation markers Prl8a2, Prl3c1 and ALP, indicating the importance of TAZ in decidualization. Knockdown of TAZ impeded HB-EGF induction of stromal cell proliferation and differentiation. Under oxidative stress, TAZ protected stromal differentiation against oxidative damage by reducing intracellular ROS and enhancing cellular antioxidant capacity dependent on the Nrf2/ARE/Foxo1 pathway. TAZ strengthened the transcriptional activity of Nrf2 which directly bound to the antioxidant response element (ARE) of Foxo1 promoter region. Additionally, silencing TAZ caused accumulation of intracellular ROS through heightening NOX activity whose blockade by APO reversed the disruption in stromal differentiation. Further analysis revealed that TAZ might restore mitochondrial function, as indicated by the increase in ATP level, mtDNA copy number and mitochondrial membrane potential with the reduction in mitochondrial superoxide. Additionally, TAZ modulated the activities of mitochondrial respiratory chain complexes I and III whose suppression by ROT and AA resulted in the inability of TAZ to defend against oxidative damage to stromal differentiation. Moreover, TAZ prevented stromal cell apoptosis by upregulating Bcl2 expression and inhibiting Casp3 activity and Bax expression. In summary, TAZ might mediate HB-EGF function in uterine decidualization through Ccnd3 and ameliorate oxidative damage to stromal cell differentiation via Nrf2/ARE/Foxo1 pathway.

Prognostic Role of C-reactive Protein in Gastric Cancer: A Meta-analysis

Yu, Qing,Yu, Xiong-Fei,Zhang, Shou-De,Wang, Hao-Hao,Wang, Hai-Yong,Teng, Li-Song Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

Background: A number of studies have investigated the association between increased pretreatment serum C-reactive protein (CRP) levels and the prognosis of gastric cancer. However, due to the inconsistent results, whether the serum CRP level can be a prognostic factor in primary gastric cancer remains controversial. Methods: We searched Medline, PubMed, Embase and the Cochrane Central Register of Controlled Trials for relevant high-quality reports. A meta-analysis was carried out using the included studies to assess the association between pretreatment serum CRP level and overall survival (OS) in patients with gastric cancer. Correlation analyses were conducted to evaluate the relationship between serum CRP and tumor characteristics such as tumor node metastasis (TNM) stage and recurrence. Results: Twelve reports involving 2,597 patients with gastric cancer were included. Primary meta-analysis indicated a significant association between elevated CRP level and poor OS (HR 1.77, 95% CI 1.56-2.00). Subgroup analyses showed no single factor could alter the primary results when we divided the included studies by "number of patients", "max follow-up period", "TNM stage", "treatment" and "cut-off value". Correlation analyses showed that serum CRP level was significantly related to TNM stage (OR 2.96, 95% CI 2.22-3.93) and tumor recurrence (OR 1.81, 95% CI 1.21-2.71). Conclusions: We demonstrated that increased pretreatment serum CRP level (${\geq}10mg/L$) was significantly associated with poor prognosis in gastric cancer patients, either in early or advanced stages.

Strategic Environmental Policy and Environmental Tariffs

( Yu Qing Xing ) 세종대학교 경제통합연구소 (구 세종대학교 국제경제연구소) 2006 Journal of Economic Integration Vol.21 No.4

This paper uses a three-stage game to analyze how environmental tariffs affect the strategic behavior of a government in designing environmental policy. The game is based on an international duopoly model with detrimental externality in production and asymmetric environmental policies between two countries. It shows that the welfare effect of the foreign country`s strategic environmental policy on the home country is ambiguous. In the circumstance that the home country would be worse off due to the lenient environmental policy of the foreign country, there exists an optimal environmental tariff. If the home country imposes the optimal tariff on the pollution-intensive imports, any deviation from the first best environmental policy by the foreign country would make the home country better off. In addition, the implementation of the environmental tariff would mitigate the motivation of the foreign country to pursue strategic environmental policy, and drive the lenient environmental standard toward the efficient level. The theoretical results imply that, in an open economy with non-harmonized environmental standards, imposing environmental tariffs on imports from the countries with lax environmental regulations would correct the adverse welfare effect, and more importantly induce the upward harmonization on environmental policy across countries.

Meta-synthesis Exploring Barriers to Health Seeking Behaviour among Malaysian Breast Cancer Patients

Yu, Foo Qing,Murugiah, Muthu Kumar,Khan, Amer Hayat,Mehmood, Tahir Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.1

Barriers to health seeking constitute a challenging issue in the treatment of breast cancer. The current meta-synthesis aimed to explore common barriers to health seeking among Malaysian breast cancer patients. From the systematic search, nine studies were found meeting the inclusion criteria. Data extraction revealed that health behavior towards breast cancer among Malaysia women was influenced by knowledge, psychological, sociocultural and medical system factors. In terms of knowledge, most of the Malaysian patients were observed to have cursory information and the reliance on the information provided by media was limiting. Among psychological factors, stress and sense of denial were some of the common factors leading to delay in treatment seeking. Family member's advice, cultural beliefs towards traditional care were some of the common sociocultural factors hindering immediate access to advanced medical diagnosis and care. Lastly, the delay in referral was one of the most common health system-related problems highlighted in most of the studies. In conclusion, there is an immediate need to improve the knowledge and understanding of Malaysian women towards breast cancer. Mass media should liaise with the cancer specialists to disseminate accurate and up-to-date information for the readers and audience, helping in modification of cultural beliefs that hinder timing health seeking. However, such intervention will not improve or rectify the health system related barriers to treatment seeking. Therefore, there is an immediate need for resource adjustment and training programs among health professional to improve their competency and professionalism required to develop an efficient health system.

miR-126 Suppresses the Proliferation of Cervical Cancer Cells and Alters Cell Sensitivity to the Chemotherapeutic Drug Bleomycin

Yu, Qing,Liu, Shan-Ling,Wang, He,Shi, Gang,Yang, Pei,Chen, Xin-Lian Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

In cervical cancer, one of the most common malignant tumors in women worldwide, miR-126 has been reported to exhibit decreased expression. However, its role in cervical cancer cell proliferation and drug sensitivity has remained relatively unexplored. Here, we compared the expression of miR-126 in cervical cancer tissues (n = 20) with that in normal cervical tissue (n = 20) using quantitative RT-PCR. The viability of Siha cervical cancer cells was further measured by MTT assay after transfection with miR-126 mimic (Siha-miR-126 mimic) or microRNA mimic negative control (Siha-miR mimic NC) and after treatment with various concentrations of bleomycin (BLM). IC50s were calculated, and the survival rates (SRs) of Siha cells were calculated. miR-126 expression in cervical cancer tissue was significantly decreased compared with that in normal cervical tissue (P < 0.01). The relative SRs of Siha-miR-126 mimic cells were also significantly decreased compared with those of Siha-miR mimic NC cells at 24-96 h after transfection. The IC50 of BLM in Siha-miR-126 mimic cells ($50.3{\pm}2.02{\mu}g/mL$) was decreased compared with that in Siha-miR mimic NC cells ($70.5{\pm}4.33{\mu}g/mL$) at 48 h after transfection (P < 0.05). Finally, the SRs of Siha-miR-126 mimic cells were significantly lower than those of SihamiR mimic NC cells after cultured in medium containing 40 ${\mu}g/mL$ BLM for 24-96 h (P < 0.05). These results suggest that miR-126 is expressed at low levels in cervical cancer. Upregulation of miR-126 inhibited cervical cancer cell proliferation and enhanced the sensitivity to BLM. Thus, miR-126 may represent a novel approach to cervical cancer treatment.