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Ahn, Heeseop,Batule, Bhagwan Sahebrao,Seok, Youngung,Kim, Min-Gon American Chemical Society 2018 ANALYTICAL CHEMISTRY - Vol.90 No.17
<P>A paper chip device-based recombinase polymerase amplification (RPA) method was developed for highly sensitive and selective single-step detection of foodborne pathogens. A paper chip was manufactured by simply stacking functional papers. RPA reagents and fluorescent probe were dried on the reaction zone of a patterned poly(ether sulfone) membrane. The RPA reaction was initiated by adding pathogen DNAs into an injection hole. Paper chip-based analysis of pathogens showed optimal performance at 37 °C for 20 min and the results were comparable to those obtained with solution-based RPA reactions. Based on the paper chip-based fluorescence signal, <I>Escherichia coli</I>, <I>Staphylococcus aureus</I>, and <I>Salmonella</I> typhimurium were simultaneously detected with detection limits of 10<SUP>2</SUP> cfu/mL. The diagnostic utility of the device was demonstrated by the reliable detection of <I>E. coli</I> and <I>S. aureus</I> present in spiked milk. This ready-to-use device could be integrated with simple nucleic acid extraction for food pathogen detection in resource-limited settings.</P> [FIG OMISSION]</BR>