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Shim, Yoon Bo,Park, Jang Su,Kang, Shin Won,Kim, Andre,Jeong, In Chul 한국생화학회 1978 Vol. No.
The interaction of cytochrome c with binary phospholipid mixtxres was investigated by solid-state ²H- and ^(31)P-NMR. To examine the effect of the interaction on the glycerol backbones, the glycerol moieties of phosphaddylcholine (PC), phosphafidylethanolamine (PE), phosphatidylglycerol (PG), and cardiolipin (CL) were specifically deuterated. On the binding of cytochrome c to the binary mixed bilayers, no changes in the quadrupole splittings of each of the components were observed for the PGPG, PE/CL and PFJ PG 6posomes. In contrast, the splittings of CL decreased on binding of protein to the PGCL liposomes, although those of PC did not change at all. This showed that cytochrome c specifically interacts with CL in PGCL bilayers, and penetrates into the lipid bilayer to some extent so as to perturb the dynamic structure of the glycerol backbone. This is distinctly different from the mode of interaction of cytochrome c with other binary mixed bilayers. In the ^(31)P-NMR spectra, line broadening and a decrease of the chemical shift anisotropy were observed on the binding of cytochrome c for all binary mixed bilayers that were examined. These changes were more significant for the PG CL bilayers. Furthermore, the line broadening is more significant for PC than for CL in PGCL bilayers. Therefore, it can be concluded that with the polar head groups, not only CL but also PC are involved in the interaction with cytochrome c.
Shim, Won-Bo,Je, Gil-Soo,Kim, Kyeongyeol,Mtenga, Adelard B.,Lee, Won-Gyeong,Song, Jeong-Un,Chung, Duck-Hwa,Yoon, Yohan Elsevier 2012 Radiation physics and chemistry Vol.81 No.5
<P><B>Abstract</B></P><P>This study evaluated effect of gamma irradiation on survival of <I>Salmonella Typhimurium</I> and <I>Staphylococcus aureus</I> on lettuce and damage of cell envelope. <I>S.</I> Typhimurium and <I>S. aureus</I> were inoculated on red leaf lettuce, and they were irradiated at 0, 0.5, 1, 1.5, 2, 2.5, and 3kGy, and the samples were then stored at 7 and 25°C for 7 days. Survival of <I>S.</I> Typhimurium and <I>S. aureus</I> were enumerated on xylose lysine deoxycholate agar and Baird–Parker agar, respectively. <I>D</I><SUB>10</SUB> value (dose required to reduce 1log CFU/leaf) was calculated, and kinetic parameters (maximum specific growth rate; <I>μ</I><SUB>max</SUB> and lag phase duration; LPD) were calculated by the modified Gompertz model. In addition, cell envelope damage of the pathogens was observed by scanning electron microscope (SEM) and transmission electron microscope (TEM). <I>D</I><SUB>10</SUB> values were 0.35 and 0.33kGy for <I>S.</I> Typhimurium and <I>S. aureus</I>, respectively. During storage at 7°C, <I>S.</I> Typhimurium and <I>S. aureus</I> had significant (<I>P</I><0.05) growth only on non-irradiated samples up to about 2.5 and 4log CFU/leaf at 0.42 and 1.28log CFU/leaf/day of <I>μ</I><SUB>max</SUB>, respectively. At 25°C, cell counts of <I>S.</I> Typhimurium and <I>S. aureus</I> on the samples irradiated at 0 and 0.5kGy increased (<I>P</I><0.05) up to 3–6log CFU/leaf. The <I>μ</I><SUB>max</SUB> of both pathogens were higher in 0kGy <I>(</I>1.08–2.27log CFU/leaf/day) and 0.5kGy (0.58–0.92log CFU/leaf/day), and LPDs ranged from 1.53 to 3.14 day. SEM and TEM observations showed that cells irradiated at 1.5 and 3kGy showed disrupted cell membrane. These results indicate that gamma irradiation could be a useful decontamination technology to improve food safety of lettuce by destroying cells of <I>S.</I> Typhimurium and <I>S. aureus</I>.</P> <P><B>Highlights</B></P><P>► Low dose of gamma irradiation destroyed cell envelope of the pathogens. ► Gamma irradiation decreased cell counts of the pathogens on lettuce. ► Gamma irradiation could be useful in improving food safety of lettuce.</P>
( Won Bo Shim ),( Boris B. Dzantiev ),( Sergei A. Eremin ),( Duck Hwa Chung ) 한국미생물 · 생명공학회 2009 Journal of microbiology and biotechnology Vol.19 No.1
Individual immunochromatographic assays (ICG) for ochratoxin A (OTA) and zearalenone (ZEA) were optimized and used in the development of a one-step simultaneous immunochromatographic assay (OS-ICG) for the rapid multianalysis of two mycotoxins in corn samples. The nitrocellulose membrane of the OS-ICG was treated with OTA-bovine serum albumin (BSA), ZEA-ovalbumin (OVA), and anti-mouse IgG in the OTA test, ZEA test, and control zones, respectively. Monoclonal antibody-gold conjugates (OTA3 MAb-gold and ZEA2C5 MAb-gold) were sprayed onto the conjugate pad. The visual detection limits were 2.5 and 5 ng/ml for OTA and ZEA, resepectively, and the results were obtained within 15 min after starting the analysis. An efficient, simple, and rapid extraction method using 30% MeOH/PBS was established and validated by analyzing the corn samples spiked with OTA/ZEA mixtures (0/0, 5/10, 10/20, and 20/30 μg/kg). The cut-off values of the OS-ICG for the spiked corn were 5 and 10 μg/kg for OTA and ZEA, respectively. Natural corn samples were analyzed by OS-ICG, direct competitive enzyme-linked immunosorbent assay (DC-ELISA), and HPLC. Results of the OS-ICG were in good agreement with those obtained by DC-ELISA and HPLC. The developed OS-ICG offers a rapid, easy-to-use, and portable analytical system and can be used as a convenient qualitative tool for the on-site simultaneous determination of OTA and ZEA in cereals, food, and agricultural products in one analytical cycle.
( Bo Young Lee ),( Jae Young Jang ),( Soung Won Jeong ),( Gene Hyun Bok ),( Jeong Ho Ham ),( Joo Young Cho ),( Joon Seong Lee ),( Chan Sup Shim ) 대한소화기기능성질환·운동학회(구 대한소화관운동학회) 2011 Gut and Liver Vol.5 No.2
We report two cases of adrenal abscesses that occurred following a Histoacryl(R) (N-butyl-2-cyanocrylate) injection for variceal bleeding. Patients had been diagnosed with alcoholic liver cirrhosis and gastric varices bleeding and received a Histoacryl(R) injection for the variceal bleeding. Patients had fever and abdominal tenderness and were diagnosed with an adrenal abscess at 2 months following the Histoacryl(R) injection. One patient received open drainage and the other underwent percutaneous drainage. When a patient has previously been injected with Histoacryl(R) for the treatment of variceal bleeding and presents with fever, an evaluation for an unusual complication such as adrenal abscess is recommended. (Gut Liver 2011;5:242-244)
Characterization of Indium Hydroxide Powders Synthesized by a Precipitation Method
Won-Jun Lee,Eun-Kyoung Choi,Ung-Soo Kim,Jong-Young Kim,Kwang Bo Shim,Hae Jin Hwang,Woo-Seok Cho 한양대학교 세라믹연구소 2017 Journal of Ceramic Processing Research Vol.18 No.11
Indium hydroxide powders were synthesized using indium nitride, and the characteristics of the indium hydroxide powder were investigated according to the precipitate aging time and the pH and concentration of the liquid synthesis medium. Indium hydroxide (In(OH)3) and indium oxide hydroxide (InOOH) phases co-existed in all conditions studied here. The crystallite size of In(OH)3 was more dependent on the concentration and pH of the reaction medium compared to that of InOOH. Hence, the relative crystallite scale of In(OH)3 increased with increasing concentration and pH of the reaction liquid more than that of in InOOH. Although the crystallite size did not change much with an increase of precipitate aging time, the specific surface area decreased greatly. Such results can aid in the synthesis of indium oxide particles that are used for producing ITO targets.
Shim, Doo-Bo,Park, Eun-Sil,Sim, Gyu-Jin,Lee, Ji-Young,Kang, Ju-Hwan,Yoo, Hyun-Joo,Choi, Yeon-Jin,Lee, Young-Mee,Lee, Sang-Yeol,Kim, Min-Gab,Kang, Da-Won,Jung, Eun-Jung,Kang, Kee-Ryeon The Korean Society for Applied Biological Chemistr 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.4
Eukaryotic translation initiation factor 5A (eIF5A) is the only hypusine-containing protein, which is formed by deoxyhypusine synthase (DHS) and deoxyhypusine hydroxylase (DOHH). DOHH is a novel metalloenzyme with HEAT [named for human huntingtin (H), elongation factor 3 (E), a subunit of protein phosphatase 2A (A), and the target of rapamycin (T)]-repeat motifs. Inspite of much progress in determining the roles of iron-containing DOHH holoenzyme as an eIF5A hydroxylase, little is known about iron-free apoenzyme of DOHH under certain stress conditions. For this purpose, we compared cell growth in two yeast strains subjected to oxidative damage. Thus, the existence of more viable cells in the Saccharomyces cerevisiae BY4743 (parental yeast) strain than in the DOHH- strain after $H_2O_2$ treatment indicates the importance of DOHH in protecting yeast cells against oxidative stress. To identify endogenous target proteins influenced by DOHH under oxidative damage, proteomic analysis was applied to the two yeast strains. Of these proteins, the oxidized form of peroxiredoxin I (PrxI) was concomitantly up-regulated in both strains under $H_2O_2$ treatment. Two-dimensional electrophoresis (DE) followed by immunoblot analysis shows that the recovery of the oxidized PrxI to the reduced enzyme under $H_2O_2$ treatment was found to be much slower in the DOHH- strain than in the parental strain. Based on the results, we discovered a possible interaction between DOHH and PrxI by immunoprecipitation/immunoblotting in yeast under oxidative stress. Taken together, these results suggest that DOHH might be a candidate protein for protection of yeast cells against oxidative stress in conjunction with PrxI.