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Lee, So Jin,Huh, Myung Sook,Lee, Seung Young,Min, Solki,Lee, Seulki,Koo, Heebeom,Chu, Jun‐,Uk,Lee, Kyung Eun,Jeon, Hyesung,Choi, Yongseok,Choi, Kuiwon,Byun, Youngro,Jeong, Seo Young,Park, Kinam WILEY‐VCH Verlag 2012 Angewandte Chemie Vol.124 No.29
<P><B>Die kondensierte Fassung</B>: Thioliertes Glycolchitosan bildet durch Ladungs‐Ladungs‐Wechselwirkungen und Netzbildung mit polymerisierten siRNAs stabile Nanopartikel (siehe Schema). Die Poly‐siRNA/Glycolchitosan‐Nanopartikel (psi‐TGC) sind in vivo genügend stabil für den systemischen Transport von siRNAs. Das Abschalten von Tumorproteinen durch psi‐TGC resultierte in reduzierten Tumorgrößen und verminderter Tumorvaskularisation.</P>
Lee, So Jin,Lee, Aeju,Hwang, Seung Rim,Park, Jong-Sung,Jang, Jiyeon,Huh, Myung Sook,Jo, Dong-Gyu,Yoon, Soo-Young,Byun, Youngro,Kim, Sun Hwa,Kwon, Ick Chan,Youn, Inchan,Kim, Kwangmeyung Academic Press 2014 Molecular therapy Vol.22 No.2
<P>Among various proinflammatory cytokines involved in the pathogenesis of rheumatoid arthritis (RA), tumor necrosis factor (TNF)-alpha, plays a pivotal role in the release of other cytokines and induction of chronic inflammation. Even though siRNA has the therapeutic potential, they have a challenge to be delivered into the target cells because of their poor stability in physiological fluids. Herein, we design a nanocomplex of polymerized siRNA (poly-siRNA) targeting TNF-alpha with thiolated. glycol chitosan (tGC) polymers for the treatment of RA. Poly-siRNA is prepared through self-polymerization of thiol groups at the 5' end of sense and antisense strand of siRNA and encapsulated into tGC polymers, resulting in poly-siRNA-tGC nanoparticles (psi-tGC-NPs) with an average diameter of 370 nm. In the macrophage culture system, psi-tGC-NPs exhibit rapid cellular uptake and excellent in vitro TNF-a gene silencing efficacy. Importantly, psi-tGC-NPs show the high accumulation at the arthritic joint sites in collagen-induced arthritis (CIA) mice. Treatment monitoring data obtained by the matrix metalloproteinase 3 specific nanoprobe and microcomputed tomography show that intravenous injection of psi-tGC-NPs significantly inhibits inflammation and bone erosion in CIA mice, comparable to methotrexate (5 mg/kg). Therefore, the availability of psi-tGC-NP therapy that target specific cytokines may herald new era in the treatment of RA.</P>
Youngro Lee,Dae-Young Lee 제어로봇시스템학회 2022 제어로봇시스템학회 국제학술대회 논문집 Vol.2022 No.11
The ability to return a sample or small payload from International Space Station to a target on the ground will be in high demand in the near future. To satisfy this demand, several universities and NASA centers have been researching such a sample return system. One of the main features of the sample return systems is that they aim to deliver payloads at a designated position on the ground without using a thruster; thus, onboard guidance is essential in improving targeting accuracy of this passive system. This paper presents a trajectory analysis to develop a novel guidance scheme that can determine an optimal ballistic coefficient and phase transition states for the passive sample return system. Numerical simulations show that the ballistic coefficient of the system highly affects the orbital decay rate and the targeting accuracy. Hence, if the novel onboard guidance scheme optimizes the ballistic coefficient and phase transition state, high targeting accuracy can be achieved. Furthermore, an onboard surrogate model that can estimate the vehicle’s ballistic coefficient air density along the trajectory will assist the onboard guidance scheme in successfully guiding the sample return system.
Lee, Esak,Kim, Yoo-Shin,Bae, Sang Mun,Kim, Sang Kyoon,Jin, Shunji,Chung, Seung Woo,Lee, Myungjin,Moon, Hyun Tae,Jeon, Ok-Cheol,Park, Rang Woon,Kim, In San,Byun, Youngro,Kim, Sang Yoon Wiley Subscription Services, Inc., A Wiley Company 2009 International journal of cancer: Journal internati Vol.124 No.12
<P>Although heparin can regulate angiogenesis, tumor growth and metastasis, its clinical application, as well as that of low-molecular heparin (LMWH), for treating cancer are limited because of heparin's anticoagulant activity and risk of hemorrhages. LMWH-taurocholate conjugates (LHT7), which have low anticoagulant activity, were synthesized. The structural property of LHT was evaluated by circular dichroism and the binding affinity of LHT7 to vascular endothelial growth factor 165 (VEGF<SUB>165</SUB>) was measured by isothermal titration calorimetry. The inhibitory effect of LHT7 on VEGF-mediated KDR (VEGF-receptor 2) phosphorylation in Human umbilical vein endothelial cells was evaluated. The VEGF<SUB>165</SUB> dependent Matrigel plug assay was performed to verify the antiangiogenic potential of LHT7 on a VEGF<SUB>165</SUB> inhibitor. Finally, tumor growth inhibition effects of LHT7 on SCC7 and the survival rate of animal models were investigated. Moreover, MDA-MB231 xenograft mouse model was additionally used to confirm the therapeutic effect of LHT7 on human breast cancer cell line. As a result, LHT7 which has 12.7% of anticoagulant activity of the original LMWH showed a peculiar polyproline-type helical structure. LHT7 binds to VEGF strongly and inhibits VEGF dependent KDR phosphorylation. The results of Matrigel plug assay proved LHT7 as a strong antiangiogenic agent inhibiting VEGF<SUB>165</SUB>. Remarkably, LHT7 showed a significant tumor growth inhibition potential on SCC7 with an increased survival rate. LHT7 also delayed tumor growth in MDA-MB231 human breast cancer cell lines. © 2008 UICC</P>
Lee, Na Kyeong,Lee, Eun Jung,Kim, Soyoun,Nam, Gi-hoon,Kih, Minwoo,Hong, Yeonsun,Jeong, Cherlhyun,Yang, Yoosoo,Byun, Youngro,Kim, In-San Elsevier 2017 Journal of controlled release Vol.267 No.-
<P><B>Abstract</B></P> <P>Ferritin nanocages are of particular interest as a novel platform for drug and vaccine delivery, diagnosis, biomineralization scaffold and more, due to their perfect and complex symmetry, ideal physical properties, high biocompatibility, low toxicity profiles as well as easy manipulation by genetic or chemical strategies. However, a short half-life is still a hurdle for the translation of ferritin-based nanomedicines into the clinic. Here, we developed a series of rationally designed long circulating ferritin nanocages (LCFNs) with ‘Intrinsically Disordered Proteins (IDP)’ as a stealth layer for extending the half-life of ferritin nanocages. Through predictions with 3D modelling, the LCFNs were designed, generated and their pharmacokinetic parameters including half-life, clearance rate, mean residence time, and more, were evaluated by qualitative and quantitative analysis. LCFNs have a tenfold increased half-life and overall improved pharmacokinetic parameters compared to wild-type ferritin nanocages (wtFN), corresponding to the low binding against bone marrow-derived macrophages (BMDMs) and endothelial cells. Subsequently, a tumor targeting moiety, epidermal growth factor receptor (EGFR)-targeting affibody peptide, was fused to LCFNs for evaluating their potential as a theragnostic platform. The tumor targeting-LCFNs successfully accumulated to the tumor tissue, by efficient targeting <I>via</I> active and passive properties, and also the shielding effect of IDP <I>in vivo</I>. This strategy can be applied to other protein-based nanocages for further progressing their use in the field of nanomedicine.</P> <P><B>Graphical abstract</B></P> <P>Long circulating ferritin nanocages are designed by 3D modelling. Modified by intrinsically disordered protein (IDP) clouds, this novel biocompatible nanocage platform can be applied in the field of nanomedicine.</P> <P>[DISPLAY OMISSION]</P>
Numerical Analysis of Relative Orbit Control Strategy for CANYVAL-X Mission
Youngro Lee,박상영,박재필,Youngbum Song 한국우주과학회 2019 Journal of Astronomy and Space Sciences Vol.36 No.4
This paper suggests a relative orbit control strategy for the CubeSat Astronomy by NASA and Yonsei using Virtual Telescope Alignment eXperiment (CANYVAL-X) mission. The main goal of CANYVAL-X is to demonstrate an essential technique, which is an arrangement among two satellites and a specific celestial object, referred to as inertial alignment, for a next-generation virtual space telescope. The inertial alignment can be accomplished by an inertial alignment system, which is a relative orbit control system combined with other subsystems, including attitude control, and the system has requirements for the relative state. Through the proposed relative orbit control strategy, consisting of separation, proximity keeping, and reconfiguration, the requirements will be satisfied. The separation direction of the two CubeSats with respect to the orbital plane is decided to provide advantageous initial condition to the orbit controller. Differential atmospheric drag control (DADC), which generates orbit control acceleration by changing the spacecraft’s effective cross section via attitude control rather than consuming propellant, is used for proximity keeping between the two CubeSats. Before the inertial alignment system is activated, reconfiguration is performed because it is difficult to meet the requirements during the proximity-keeping stage. Numerical simulations show that the requirements of the inertial alignment system can be satisfied by the relative orbit control strategy. Furthermore, through numerical simulations, it is demonstrated that the inertial alignment can be achieved using the inertial alignment system when the requirements are satisfied. We had been receiving a beacon signal for several months after the launch; however, we have lost the signal completely at present.
Lee, Seulki,Kim, Sang Kyoon,Lee, Dong Yun,Chae, Su Young,Byun, Youngro American Society for Microbiology 2006 Antimicrobial agents and chemotherapy Vol.50 No.5
<B>ABSTRACT</B><P>Oral administration of ceftriaxone associated with a bile acid-based new oral carrier, cholylethylenediamine, in 50% propylene glycol to rats at doses of 25 and 50 mg/kg of body weight resulted in a significant increase in intestinal absorption, as evidenced by 55% improvement in the bioavailability, whereas ceftriaxone alone showed a bioavailability of less than 1%.</P>
Induced Phenotype Targeted Therapy: Radiation-Induced Apoptosis-Targeted Chemotherapy
Lee, Beom Suk,Cho, Yong Woo,Kim, Gui Chul,Lee, Do Hee,Kim, Chang Jin,Kil, Hee Seup,Chi, Dae Yoon,Byun, Youngro,Yuk, Soon Hong,Kim, Kwangmeyung,Kim, In-San,Kwon, Ick Chan,Kim, Sang Yoon U.S. Dept. of Health, Education, and Welfare, Publ 2015 Journal of the National Cancer Institute Vol.107 No.2
<P><B>Background:</B></P><P>Tumor heterogeneity and evolutionary complexity may underlie treatment failure in spite of the development of many targeted agents. We suggest a novel strategy termed induced phenotype targeted therapy (IPTT) to simplify complicated targets because of tumor heterogeneity and overcome tumor evolutionary complexity.</P><P><B>Methods:</B></P><P>We designed a caspase-3 specific activatable prodrug, DEVD-S-DOX, containing doxorubicin linked to a peptide moiety (DEVD) cleavable by caspase-3 upon apoptosis. To induce apoptosis locally in the tumor, we used a gamma knife, which can irradiate a very small, defined target area. The in vivo antitumor activity of the caspase-3–specific activatable prodrug combined with radiation was investigated in C3H/HeN tumor-bearing mice (n = 5 per group) and analyzed with the Student’s <I>t</I> test or Mann-Whitney U test. All statistical tests were two-sided. We confirmed the basic principle using a caspase-sensitive nanoprobe (Apo-NP).</P><P><B>Results:</B></P><P>A single exposure of radiation was able to induce apoptosis in a small, defined region of the tumor, resulting in expression of caspase-3. Caspase-3 cleaved DEVD and activated the prodrug. The released free DOX further activated DEVD-S-DOX by exerting cytotoxic effects on neighboring tumor or supporting cells, which repetitively induced the expression of caspase-3 and the activation of DEVD-S-DOX. This sequential and repetitive process propagated the induction of apoptosis. This novel therapeutic strategy showed not only high efficacy in inhibiting tumor growth (14-day tumor volume [mm<SUP>3</SUP>] vs radiation alone: 848.21±143.24 vs 2511.50±441.89, <I>P</I> < .01) but also low toxicity to normal cells and tissues.</P><P><B>Conclusion:</B></P><P>Such a phenotype induction strategy represents a conceptually novel approach to overcome tumor heterogeneity and complexity as well as to substantially improve current conventional chemoradiotherapy with fewer sequelae and side effects.</P>