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Son, Tae Il,Park, Se Hoon,Kang, Hahk Soo,Son, Young Suk,Kim, Chun Ho,Jang, Eui-Chan 한국공업화학회 2005 Journal of Industrial and Engineering Chemistry Vol.11 No.1
Low-molecular-weight chitosan (LMC) having molecular weight of 10535 was obtained through acid hydrolysis of crude chitosan (CS-0) from shrimp shells and subsequent ultra-filtration. LMC was characterized by FT-IR, ¹H NMR spectroscopy and gel permeation chromatography (GPC). To stabilize the three-dimensional structure of the EGF moiety, EGF was attached to LMC using a water-soluble carbodiimide (EGF-LMC). The amount of EGF in EGF-LMC, after purification using dialysis tubing (molecular weight cut-off 12000 Da), was measured by indirect enzyme-linked immunosorbent assay (ELISA). Cytotoxicity and relative cell proliferation tests of with added EGF-LMC were performed on human dermal fibroblasts. No inhibitory effects of EGF-LMC were found during cell growth. EC2000 (EGF-LMC added with 0.338 nmol of EGF) appeared to have a stronger stimulatory effect than did E200 on fibroblast proliferation over a 96 h incubation period. Therefore, we believe that EGF-LMC is a potent wound-healing agent because of its mitogenicity and potent efficiency.
Polystyrene-Divinyl benzene copolymer를 담체로 한 oligoglucosamine 유도체의 제조 및 흡착특성
박세훈 ( Se Hoon Park ),강학수 ( Hahk Soo Kang ),이영기 ( Young Gi Lee ),손태일 ( Tae Il Son ) 한국키틴키토산학회 2003 한국키틴키토산학회지 Vol.8 No.1
N/A Chitosan was prepared by deacetylation of natural chelate polymer, chitin, with 40% aqueous sodium hydroxide solution and oligoglucosamine was prepared by degradation of chitosan with concentrated HCI. New glucosamine series chelate resin(SG-resin) was synthesized by chloromethylation of styrene-DVB copolymer(3% DVB) with chloromerhylmethyl ether and SnCl4, followed by reacting of oligoglucosamine with chloromethylated copolymer. Absorptivities of the heavy metallic ions such as Hg^(++), Pb^(++), Cr^(++), and Cr^(++) ions to chitin, chitosan, SG-resin were investigated with variation of pH. In the case of chitin, the adsoptivity at below pH 5.8 was in order of following sequence Ph^(++)>Cd^(++)>Hg^(++)>Cr^(+6) and showed selectivity(100%) at above pH 4.9 and suddenly decreased at below this pH. To Cd^(++) ion the absorptivity tendency showed maximum at pH 4.0. Comparing the absorptivity of the chitosan with that of the chitin, it showed that ahsorptivity of chitosan was improved more than that of chitin, at above pH 2.5 in order of following sequence Hg^(++)>Ph^(++)>Cd^(++)>Cd^(+6), Absorptivity to Hg^(++) ion showed about 90% independent on pH variation. Absqtivity of the SG-resin was in the order following sequence : Pb^(++)>Hg^(++)>Cd^(++)>Cr^(+6) at above pH 2.3 and decreased comparing with that of the chitin or chitosan. To Pb^(++), Hg^(++), Cd^(++) ion the adsorption tendency was improved as the pH value war increased. In the adsorption excellent of Cd^(++), Cr^(+6) ions (100 ppm) to the chitin, chitosan, SG-resin under coexistence with 1.000 ppm of Na^(+) and Ca^(++) ions, respectively, it was observed that the absorptivity of Cd^(++), Cr^(+6) ions were not affected by these common ions.