집먼지진드기에 대한 강화약쑥 추출액의 기피효과

안윤주, 지차호 충북대학교 동물의학연구소 2009 Journal of Biomedical and Translational Research Vol.10 No.1

본문참고

EFFECT OF EUGENOL AND CAPSAICIN ON THE VOLTAGE-DEPENDENT ION CHANNELS OF TRIGEMINAL AFFERENTS

김주연,박상진,최기운,최호영 대한치과보존학회 2000 Restorative Dentistry & Endodontics Vol.25 No.3

삼차신경절의 뉴런이 구강악안면영역에서의 촉각, 압각,온도각 및 통각 등 다양한 감각을 중추신경계로 전달하는 역할을 하는 것은 주지의 사실이다. 이러한 신경전달에 있어서 이온통로는 감각정보를 전달하는데 핵심적인 역할을 수행하며 특히 소디움 통로는 활동전위의 발생에 중요하다. 소디움 통로는 tetrodotoxin-sensitive(TTX-5) 및 tetrodotox-in-resistant(TTX-r) 통로로 나누어지는 데 이 중 TTX-r 통로에 발생되는 tetrodotoxin-resistant sodium cur-rent(TTX-r INa.)는 capsaicin에 민감한 일차구심신경세포에서 유해자극에 의해 통각신호를 발생시키고 전달하는데 중요하다. 또한 칼슘 통로는 시냅스 전토에 있어서 필수적인 역할을 수행하고 있다. 한편 치과영역에서 치수의 진정 목적으로 eugenol이 흔히 사용되고 있다. 그러나eugenol의 그 작용 기전에 대해서 현재까지 이온통로에 대한 상세한 결과가 없는 실정이며 최근의 보고에 의하면 eugenol이 capsaicin수용기를 통하여 감각신경에 대한 억제작용을 나타낸다고 한다. 따라서 본 실험은 eugenol과 capsaicin이 흰쥐의 삼차신경절의 TTX-r INa와 칼슘통로에 어떠한 영향을 미치는지를 알아보고 eugenol이 capsaicin 수용기를 통하여 작용하는지를 검증하고자 시행되었다 삼차신경절 뉴런은 100-150g의 흰쥐의 삼차신경절로부터 외과적으로 절제하여 통법의 화학적 및 기계적 처리를 통해 단일세포로 분리하였고 이를 Whole-cell patch clamp 방법을 이용하여 시행한 바 다음과 같은 결론을 얻었다. 1.1mM의 dugenol은 흰쥐 삼차신경절 뉴런의 TTX-r INa.와 HVA INa.를 억제하였다. 2.1㎛의 capsaicin은 흰쥐 삼차신경절 뉴런의 TTX-r INa 와 HVA INa 를 억제하였다 3.Capsazepine은 capsaicin의 HVA ICa 에 대한 억제작용을 차단하였다. 4.Capsazepine은 capsaicin의 HVA ICa .에 대한 억제작용을 차단하지 못하였다. 결론적 으로 eugenol과 capsaicin은 tetrodotoxin-resistant sodium current(TTX-r INa )와 high voltage-activated calcium current(HVA ICa )를 모두 억제하는 것으로 나타났으며 , 이러한 작용이 통각의 발생과 시냅스 전달과정을 차단하여 치수 진정 목적으로 많이 사용하는 eugenol의 작용기전으로 판단된다. 한편 capsaicin의 길항제인 capsazepine을 전처치하였을 때에도 eugenol의 HVA ICa 에 대한 억제효과는 변화가 없었다. 이와같은 결과로 보아 HVA ICa 에 관한한 eugenol은 capsaicin 수용기를 통하여 나타나지 않는 것으로 사료된다.

담양신설 IC의 교통소음도 조사연구

최형일,강영주,채희정,김연풍,조도호,설동화,정숙경 조선대학교 환경공해연구소 2007 環境公害硏究 Vol.20 No.1

After the vehicle has become a large formation with high-speed, loud noise from an express highway is increasing, and the number of large-scale apartment complexes, which are recently being constructed in the metropolitan area nearby the express highway and IC, is increasing gradually. Compared with a main road, the traffic volume and the speed of a vehicle between IC and the ramp way [turn-off] are smaller. If the ramp way is rightly connected with the downtown area, however, the traffic-noise that comes about in surrounding areas will be in conjunction with the traffic-noise from the main road of express highway and that of the ramp way. This research has selected a place that hasn't had a road or a commercial quarter nearby the main-road of IC and ramp way. Then we have studied the traffic-noise status under minimizing influence of its outer-noise, which occurs in IC. 1. The traffic volume change of a small and full-sized car with time has been kept on regularity in 2004 and 2005. As the traffic volume of a full-sized car has diminished remarkably, it has been maintaining through 6:00a.m.-10:00 a.m. As it has decreased since lO:OO a.m.. we have also observed the traffic proportion going up. 2. Monthly traffic volume of small cars was higher in May, August and October than other months. We have observed that the high traffic volume during these months is due to the following: May-The Damyang festival, August-a high-demand season, October-viewing scarlet maple leaves. Monthly traffic volume of full-sized cars was generally higher in August, but it has decreased during 2006. This was due to the many construction trucks that were working on the extension of the 88 Olympic expressway. Therefore, there was more traffic volume in 2006 compared to 2004 and 2005. 3. In this study, the noise figures have been less than 55 dB(A) in 18 spots. There were 14 spots from 55dB(A) to 58dB(A). 58-61dB(A) : 20, 61-64dB(A) : 17, 64-67dB(A) : 18. There were 8 spots we observed at the highest level from 67dB(A) to 70dB(A). Those were the nearby spots separating the main road and Damyang IC. 4. Traffic-noise at the boundary between IC and the main road of an expressway was higher than that of the same distance in other places. 5. We have observed that traffic-noise is low in places with mountains that serve the role of soundproofing walls. Thus, the fundamental sound hadn't affected it.

월출산국립공원 수질특성에 관한 연구

최형일,김난희,강영주,채희정,김연풍,조도호,설동화 조선대학교 환경공해연구소 2007 環境公害硏究 Vol.20 No.1

Because mountain streams have narrow widths and steep slopes compared to streams and lakes, hours of immobility are considerably short, and the quality of mountain streams has been greatly influenced by rainfall, land-use, components of the streambed and types of forests. During the summer when heavy rainfall occurs, eroded earth and sand flow into mountain streams and cause temporarily high turbidity. It is also the cause of eutrophication, which is frequently found in downstream. As evaluation standards for the quality of mountain streams were not established in Korea, this quality was evaluated by examining some items based on quality standards for drinkable water and research on the influences of pollution in mountain streams, which showed that these were seldom in downstream water systems. Mountain streams within Wolchulsan National Park flow into a reservoir and when characteristics of mountain streams from forests and rainfall that flow into mountain streams through forest ecosystems were studied, results of measuring its changes are as follows. Monthly changes of pH were low between April and August and higher starting October, and the EC was measured higher in the winter and the spring and tended to be low starting April. The EC on the 19th of May and the 23rd of October and the concentration of floating materials and COD_(Mn) were observed to be higher due to the influence of rainfall on the 19th of May and the 22nd of October, and it was judged that the washing out of dried deposits attached to the water pipe and the leaching of woods were influenced by rainfall. It is considered that real-time monitoring of water quality for a long time and water quality conditions during and after rainfall should be examined for ensuring basic data of water quality and evaluating characteristics and seasonableness of mountain streams, which may be greatly changed by artificial influence in the future.

약초 추출액에 의한 향특소포자충 효과 및 대식세포의 면역활성

양용석,김인식,강성구,박주연,성호중,남호우,하지용 연세대학교 보건과학연구소 2001 보건과학논집 Vol.11 No.-

Effects of Tributyltin Chloride (TBTC1) on Development of Reproductive Organs and Expression of Steroidogenic Enzymes in Male rats

Ho Youn Ki,Su Jung Lee,Jae Ho Shin,Il Hyun Kang,Hyun Ju Moon,Tae Sung Kim,Hoon Bae,Mi Sook Dong,Yong-Dal Yoon,Soon Young Han 환경독성보건학회 2004 한국독성학회 심포지움 및 학술발표회 Vol.- No.-

국내 건축물의 피난안정성평가를 위한 비상대피 시 재실자 이동속도에 관한 연구

Ho-Ju Youn,EunKyung Hwang 한국재난정보학회 2013 한국재난정보학회 논문집 Vol.9 No.3

최근 건축물 내의 피난안전성을 확보하고자 계획 및 설계단계부터 컴퓨터 시뮬레이션을 활용한 검토와 피난평가를 수행하고 있다. 이러한 평가는 초기입력요소에 따라 결과 값이 달라지기에 무엇보다 요소별 Data구축이 중요하지만, 국내는 피난관련 실험 및 연구가 미흡해 대부분이 국외기준을 준용하고 있는 게 현실이다. 이에 본 연구는 국내 재실자 이동속도 Data 구축의 목적으로 연령별 피난이동속도실험을 수행 분석해 보았다. 먼저 국내외 피난이동속도 관련 연구를 분석했으며 국내인 134명을 상대로 피난이동속도 실험을 시행하였다. 이를 바탕으로 실험으로 도출된 이동속도 결과치와 기존의 국외 이동속도와 비교 분석하여 국내피난상황과의 검토 및 확인절차를 실시하였다. 앞으로 이렇게 도출된 실험 Data는 국내 피난안정성평가의 이동속도 관련 기초자료로 활용될 것으로 판단된다.

Identification of lipopolysaccharide-binding peptide regions within HMGB1 and their effects on subclinical endotoxemia in a mouse model

Youn, Ju Ho,Kwak, Man Sup,Wu, Jie,Kim, Eun Sook,Ji, Yeounjung,Min, Hyun Jin,Yoo, Ji-Ho,Choi, Ji Eun,Cho, Hyun-Soo,Shin, Jeon-Soo WILEY-VCH Verlag 2011 European journal of immunology Vol.41 No.9

<P>Lipopolysaccharide (LPS) triggers deleterious systemic inflammatory responses when released into the circulation. LPS-binding protein (LBP) in the serum plays an important role in modifying LPS toxicity by facilitating its interaction with LPS signaling receptors, which are expressed on the surface of LPS-responsive cells. We have previously demonstrated that high mobility group box 1 (HMGB1) can bind to and transfer LPS, consequently increasing LPS-induced TNF-α production in human peripheral blood mononuclear cells (PBMCs). We report here on the identification of two LPS-binding domains within HMGB1. Furthermore, using 12 synthetic HMGB1 peptides, we define the LPS-binding regions within each domain. Among them, synthetic peptides HPep1 and HPep6, which are located in the A and B box domains of HMGB1, bind to the polysaccharide and lipid A moieties of LPS respectively. Both HPep1 and HPep6 peptides inhibited binding of LPS to LBP and HMGB1, LBP-mediated LPS transfer to CD14, and cellular uptake of LPS in RAW264.7 cells. These peptides also inhibited LPS-induced TNF-α release in human PBMCs and induced lower levels of TNF-α in the serum in a subclinical endotoxemia mouse model. These results indicate that HMGB1 has two LPS-binding peptide regions that can be utilized to design anti-sepsis or LPS-neutralizing therapeutics.</P>

Preparation and Characterization of Self-assembled Glycol Chitosan Hydrogels Containing Palmityl-acylated Exendin-4 for Extended Hypoglycemic Action

( Ju Ho Lee ),( Chang Kyu Lee ),( Sung Ho Bae ),( Jeong Hyun Yoon ),( Eun Joo Choi ),( Kyung Taek Oh ),( Eun Seong Lee ),( Kang Choon Lee ),( Yu Seok Youn ) 한국약제학회 2011 Journal of Pharmaceutical Investigation Vol.41 No.3

Injectable chitosan hydrogels have attracted great potential due to sustained-release property and safety. Here, palmityl-acylated glycol chitosan (Pal-GC) was used to generate physically cross-linked hydrogels by virtue of hydrophobic attraction of linear fatty carbons. Glycol chitosan was chemically modified with N-hydroxysuccinimide-activated palmitic acid in dimethylsulfoxide (DMSO) containing dimethylaminopyridine. Through a series of preparation steps of (i) dialysis with DMSO, (ii) addition of palmityl-acylated exendin-4 (Ex4-C16), and (iii) dialysis with water, Pal-GC was selfassembled to form physically cross-linked hydrogels entrapped with Ex4-C16. The Pal-GC derivative was analyzed by using 1H NMR, and the surface morphology of Pal-GC hydrogels formed was examined by scanning electron microscopy. Also, the hypoglycemic effect induced by Pal-GC hydrogels containing Ex4-C16 (250 nmol/kg) was evaluated in non-fasted type 2 diabetic db/db mice and compared with GC hydrogels containing native Ex4 at the same dose. Results showed that palmityl group was successfully conjugated with the amines of glycol chitosan, and that Pal-GC efficiently generated the hydrogels formation. Moreover, Pal-GC hydrogels containing Ex4-C16 was found to greatly prolong the hypoglycemia duration (~ 4 days). This was due to the dual-functions of the palmityl groups present in both GC and exendin-4 such as hydrophobic attraction and plasma albumin-binding. We consider this new type of self-assembled GC hydrogels loaded with Ex4-C16 would be a promising long-acting sustained-release system with anti-diabetic property.

A Treatment for Truncated Boundary in a Half-Space with 2-D Rayleigh Wave BEM

Ju, Tae-Ho,Cho, Youn-Ho,Phan, Haidang,Achenbach, Jan D. The Korean Society for Nondestructive Testing 2011 한국비파괴검사학회지 Vol.31 No.6

Analysis of two-dimensional Rayleigh wave scattering pattern by a surface defect is studied through modified boundary element method. BEM proposed in this paper has special treatment at each end of boundary which should have the Rayleigh wave go away without any generation of virtual reflections. It is shown that treatment for truncated boundary which is used to model two-dimensional Rayleigh waves' behavior in an elastic half-space is successfully implemented. To check numerical results' accuracy, time domain IFFT signal of the displacements is presented. Improvement on getting rid of unwanted influence of truncated boundary induced by 2-D Rayleigh waves on a flat surface of an elastic half-infinite medium is shown. As a final goal, the numerical results of Rayleigh wave scattering trend are plotted and they are compared with theoretical curves to prove its accuracy.