Zeolite-Mediated Cation Exchange Enhances the Stability of mRNA during Cell-Free Protein Synthesis

Kim, You-Eil,Kim, Dong-Myung,Choi, Cha-Yong The Korean Society for Biotechnology and Bioengine 2006 Biotechnology and Bioprocess Engineering Vol.11 No.3

The addition of zeolite particles enhances the stability of mRNA molecules in a cell-free protein synthesis system. When $20{\mu}g/{\mu}L$ of zeolite (Y5.4) is added to a reaction mixture of cell-free protein synthesis, a substantial increase in protein synthesis is observed. The stabilizing effect of zeolite is most dearly observed in an in vitro translation reaction directed by purified mRNA, as opposed to a coupled transcription and translation reaction. Upon the addition of zeolite in the in vitro translation reaction, the life span of the mRNA molecules is substantially extended, leading to an 80% increase in protein synthesis. The effect of zeolite upon the mRNA stability appears be strongly related to the cation exchange (potassium to sodium) reaction. Our results demonstrate the possibility of modifying this biological process using heterogeneous, non-biological substances in a cell-free protein synthesis system.

해외서지정보를 활용한 북한 과학기술 연구개발 트렌드 변화분석(1986~2019)

김유일(You Eil Kim) 한국콘텐츠학회 2021 한국콘텐츠학회논문지 Vol.21 No.4

대외적인 활동이 거의 없던 북한에서 최근 해외 논문발표가 급증하고 있다. 본 연구에서는 북한의 해외발표 논문을 계량분석하여 북한의 연구개발 동향변화를 파악하고, 향후 국내 연구자들과의 공동연구로 발전시킬 수 있는 가능성을 모색하고자 한다. 이를 위해 1986년 이후 북한 연구자가 포함된 해외발표논문을 대상으로 빈도분석과 네트워크 분석을 실시하였다. 분석은 저자, 연구기관, 국가, 연구분야 등을 대상으로 하였으며, 김정은 집권 전과 후로 구분하여 변화를 분석하였다. 분석결과에 따르면 김정은 집권 후 8년간 해외논문 발표가 급격히 증가하면서, 논문발표 상위 연구자나 연구기관, 공동연구 영향력 상위 연구자 등의 구성도 많은 변화가 나타났다. 김정은 집권 후 북한 연구자나 연구기관의 비중이 상대적으로 증가하였으며, 공동연구 영향력 상위 연구자들간 네트워크 컴포넌트가 형성되어 있어서 특정 연구주제에 집중하는 모습이 관찰되었다. 북한 연구자들의 주요한 해외 협동연구자나 연구기관은 중국의 비중이 높게 나타났다. In North Korea, where there was little external activity, the number of overseas academic paper publications has recently increased. This study aims to quantitatively analyze North Korean papers published abroad to identify changes in North Koreas research and development trends, and to explore the possibility of developing them through joint research with domestic researchers in the future. To this end, since 1986, frequency analysis and network analysis have been conducted on papers published overseas including North Korean researchers. The analysis was conducted on authors, research institutes, countries, and research fields, and changes were analyzed before and after Kim Jong-un took office. According to the results of the analysis, as the number of foreign papers published rapidly increased during the eight years after Kim Jong-un took office, the composition of the top researchers, research institutes, and researchers with high influence in joint research also changed a lot. After Kim Jong-un came to power, the proportion of North Korean researchers and research institutes increased relatively, and a network component was formed between researchers with high influence on joint research, so it was observed that they focused on specific research topics. It was found that the major foreign collaborators or research institutes of North Korean researchers were from China.

Zeolite-Mediated Cation Exchange Enhances the Stability of mRNA during Cell-Free Protein Synthesis

김동명,최차용,You-Eil Kim 한국생물공학회 2006 Biotechnology and Bioprocess Engineering Vol.11 No.3

The addition of zeolite particles enhances the stability of mRNA molecules in a cell-free protein synthesis system. When 20 μg/μL of zeolite (Y5.4) is added to a reaction mixture of cell-free protein synthesis, a substantial increase in protein synthesis is observed. The stabilizing effect of zeolite is most clearly observed in an in vitro translation reaction directed by purified mRNA, as opposed to a coupled transcription and translation reaction. Upon the addition of zeolite in the in vitro translation reaction, the life span of the mRNA molecules is substantially extended, leading to an 80% increase in protein synthesis. The effect of zeolite upon the mRNA stability appears be strongly related to the cation exchange (potassium to sodium) reaction. Our results demonstrate the possibility of modifying this biological process using heterogeneous, non-biological substances in a cell-free protein synthesis system.

골프지도자의 역할지각 및 직무만족과의 관계

백병주,이근일,유승원 龍仁大學校 2003 용인대학교 논문집 Vol.21 No.-

This study purposes to find out the relationship between role awareness and self-realization according to the types of golf instructors. To do this, I made analyses on three point---the difference between role awareness and position satisfaction in accordance with the social and demographic characteristics of golf teachers, the difference between role awareness and position satisfaction under the qualified types of golf teachers, and finally the influences to position satisfaction by the role awareness of the golf teachers. The study was surveyed on 250 golf teachers in indoor or outdoor golf rages or sports centers in Seoul and Gyeonggi Province by a random cluster sampling and actually used data were from 228 respondents excluding 22 persons who were considered to respond insincerely or omit some parts. The statistic analysis methods used in the study are correlation and regression. I finally got the following results in this study through the data analysis from the above-stated methods and procedure. among the influential factors which role awareness gives to position satisfaction, it shows the significant level at 5 percent in role conflicts in fellowship, working environment, and payment, in vague roles in fellowship, working environment, payment and promotion and the excessive roles in work itself. working environment, and promotion. In the case of position satisfaction, it showed description 69.0%(R^2=.690) in role conflict, 73.6%(R^2=.736) in vague roles, and 49.3%(R^2=.493) in excessive roles.

온라인 과학기술정보자원의 분류체계에 대한 실천적 구성방안

김유일,최성배,구영덕,Kim, You-Eil,Choi, Sung-Bae,Koo, Young-Duk 한국과학기술정보연구원 과학기술정보센터 2006 Journal of Information Science Theory and Practice Vol.37 No.4

인터넷의 등장은 과학기술정보의 생산과 유통에 많은 변화를 초래하고 있다. 기존 과학기술정보들은 주로 문헌위주로 생산되었다. 그러나, 인터넷이 대중화되면서 온라인 과학기술정보들이 대량으로 생산되고 있으며, 그 활용이나 중요도 또한 과학기술분야의 문헌에 뒤지지 않고 있다. 온라인 과학기술정보의 양적 질적 성장으로 인해, 정보서비스기관들은 이들 정보들의 체계적인 관리와 효율적인 서비스를 고민하고 있으며, 다양한 정보관리방법을 모색하고 있다. 효율적인 정보 관리를 위한 방법 중의 하나인 분류체계는 주요한 이슈이며, 지금까지는 문헌정보 중심의 분류체계나 일반 웹사이트의 분류체계를 벤치마킹하여 온라인 과학기술정보를 위해 도입되는 경우가 많았다. 본고에서는 국가 차원의 표준분류를 이용하여, 온라인 과학기술정보를 위한 분류체계 구성방안을 실천적으로 제시하여 보았다. The advent of the internet has caused a number of changes in production and dissemination of science and technology information(STI). STI was mainly produced in the form of publication before the advent of the internet. The popularization of the internet, however, has induced the mass production and distribution of STI through the internet. In consequence, the importance and usefulness of on-line STI have become on a level with those of published STI. The rapid growth of quantity and quality of on-line STI forces information service organizations try their best for improving the service satisfaction. In an effort to improve the satisfaction, researchers on information management examined the classification of information resources by exploring the counterparts of bibliography and/or web information service. We examined the national standard of the classification related to science and technology and proposed a practical scheme for the classification of on-line STI resources.

Weak Signal 탐색을 위한 연구개발 환경변화 분석모델 개발

홍성화(Sung-Wha Hong),김유일(You-Eil Kim),배국진(Kuk-Jin Bae),박영욱(Young-Wook Park),박종규(Jong-Kyu Park) 한국기술혁신학회 2009 기술혁신학회지 Vol.12 No.1

본 연구에서는 최근 변화무쌍한 미래 환경에 대처하기 위한 세부적인 환경분석 방법론의 하나로 활발하게 연구되고 있는 weak signal에 대한 탐색 프로세스를 체계화 하고자 하였다. 또한 글로벌 모니터링, 트렌드 분석, 브레인스토밍, 델파이 등의 기법을 토대로 한 정성적 방법과 weak signal tracking board의 개발을 통한 정량적인 방법을 적용하여, 미시적인 환경변화 분석 프로세스(NEST : New & Emerging Signals of Trends)와 이를 적용한 KISTI 이머징 시그널 분석 결과를 제시 하고자 하였다. 구체적으로 환경변화 관련 씨앗 정보를 탐색하기 위한 글로벌 모니터링 체제, weak signal 트래킹 보드를 활용한 약한 신호 분석 단계, study-watch 프레임워크를 토대로 한 up-coming trend 스캐닝 단계, 연구 결과의 객관화를 위한 전문가들의 델파이 평가 단계 등으로 세부 모듈을 설계하였다. 본 프로세스의 결과물인 미래에 큰 변화를 가져올 가능성이 있는 유망기술의 씨앗정보인 weak signal과 새로운 트렌드의 형성 가능 초기조짐정보인 up-coming trend는 우리나라의 차세대 수종 산업(유망기술)을 선정하는 데 활용가능하다. 특히 NEST 프로세스는 소규모 인력으로도 효율성을 발휘할 수 있는 weak signal 탐색 체제로 기술혁신 기업들도 독자적인 NEST 프로세스 개발이 가능할 것이다. The importance of searching the weak signal has been increasingly recognized to cope with rapidly changing circumstances as an environmental analysis technique. This study proposed the NEST process for the searching for the weak signal. The NEST (New & Emerging Signals of Trends) is a micro environmental analysis process based on both quantitative and qualitative method. For this, the weak signal Searching Board is developed and traditional methods as global monitoring, trend analysis, brainstorming and delphi method are implemented to NEST. The NEST process is consists of three stage modules; the global monitoring stage in search of seeds information related to the environmental change, the weak signal analysis stage using the weak signal Tracking Board, and the delphi valuation stage for objectifying the final result. The NEST provides the weak signal of the promising technology which can bring new paradigm and the Up-Coming Trends which can lead new trend in the future. These outputs can be used to select promising technology from firm level to national level. The NEST system can be effectively operated as well as in small group so that small and medium innovative firms can develop and execute their own NEST process individually.

대한민국 성인 남녀의 피부암에 대한 인식도 설문조사

최윤림 ( Yun Lim Choi ),유신애 ( Shin Ae You ),이동윤 ( Dong Youn Lee ),이주흥 ( Joo Heung Lee ),양준모 ( Jun Mo Yang ),이일수 ( Eil Soo Lee ),김원석 ( Won Serk Kim ) 대한피부과학회 2006 대한피부과학회지 Vol.44 No.4

웹 검색트래픽을 활용한 소비자의 기대주기 비교 연구

전승표(Seung-Pyo Jun),김유일(You Eil Kim),유형선(Hyoung Sun Yoo) 한국기술혁신학회 2013 기술혁신학회지 Vol.16 No.4

신기술을 발견하고, 사회적 변화를 예측ㆍ설명하기 위해 여러 가지 형태의 기술 수명주기 모델이 개발되어 활용되어 왔는데, 그 중에서 가트너(Gartner)사가 소개한 기술 기대주기(hype cycle) 이론은 현장에서 자주 활용되고 있으며 그 효과를 인정받고 있다. 그러나 널리 통용되는 인기에 비해서 현재 연구 문헌에서는 이론적 프레임과 관계 및 실증에 대한 고려가 부족했다. 본 연구에서는 이런 기대주기 모델의 새로운 실증적 측정 방법으로 제시된 웹 검색트래픽을 활용해 국내와 해외의 기대주기를 비교했다. 기존 연구에서 특정 제품이나 특정 국가에만 한정되었던 기대주기 분석을 검색트래픽을 활용해 비교 분석을 시도했다. 먼저 동일 제품에 대한 국가간 기대주기의 차이를 확인하기 위하여 검색트래픽과 신차판매점유율을 비교했는데, 국가간 기대주기의 유사성을 확인했으며, 통계적인 유의미성도 확인했다. 다음으로 동일 국간에서 제품간 기대주기의 차이를 확인하기 위하여 검색트래픽과 보급률을 비교해 보았는데, 역시 버블기의 정점 시기라는 측면에서 제품간 기대주기의 유사성도 확인했다. 본 연구는 검색트래픽을 활용한 사회현상 설명이나 기대주기 모델의 객관성과 설명력을 높이는데 크게 기여할 수 있으며, 나아가 마케팅 전략 수립과 같은 실질적인 기업전략 수립에까지 기여할 수 있을 것으로 판단된다. In an effort to discover new technologies and to forecast social changes of technologies, a number of technology life-cycle models have been developed and employed. The hype cycle, a graphical tool developed by a consulting firm, Gartner, is one of the most widely used models for the purpose and it is recognised as a practical one. However, more research is needed on theoretical frames, relations and empirical practices of the model. In this study, hype cycle comparisons in Korean and global search websites were performed by means of web-search traffic which is proposed as an empirical measurement of public expectation, analysed in a specific product or country in previous researches. First, search traffic and market share for new cars were compared in Korea and the U.S. with a view to identifying differences between the hype cycles in the two countries about the same product. The results show the similarity between the two countries with the statistical significance. Next, comparative analysis between search traffic and supply rate for several products in Korea was conducted to check out their patterns. According to the analysis, all the products seem to be at the “Peak of inflated expectations” in the hype cycles and they are similar to one another in the hype cycle. This study is of significance in aspects of expanding the scope of hype cycle analysis with web-search traffic because it introduced domestic web-search traffic analysis from Naver to analyse consumers" expectations in Korea by comparison with that from Google in other countries. In addition, this research can help to explain social phenomina more persuasively with search traffic and to give scientific objectivity to the hype cycle model. Furthermore, it can contribute to developing strategies of companies, such as marketing strategy.

특허정보를 활용한 바이오에너지 기술경쟁력 분석

장혜정(Hye-Jeong Jang),김유일(You-Eil Kim),정대현(Dae-Hyun Jung),권영일(Young-Il Kwon) 한국콘텐츠학회 2013 한국콘텐츠학회 종합학술대회 논문집 Vol.2013 No.5

Surgical Treatment of Axillary Osmidrosis: A Study of 160 Axillae Comparing Subdermal Excision with Liposuction and Dermal Curettage

( Nark Kyoung Rho ),( Dong O You ),( Beom Jun Kim ),( Won Serk Kim ),( Joo Heung Lee ),( Jun Mo Yang ),( Eil Soo Lee ) 대한피부과학회 2006 대한피부과학회 학술발표대회집 Vol.58 No.2