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RISS 인기검색어
- 검색키워드
- 저자 : Yongfeng Deng (검색결과 4 건)
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Yan Guo,JInliang Zhang,Yongfeng Yan,Jian Wu,Nengwu Zhu,Changyan Deng 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.6
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and subsequent sub-cloning and sequencing were used in this study to analyze the molecular phylogenetic diversity and spatial distribution of bacterial communities in different spatial locations during the cooling stage of composted swine manure. Total microbial DNA was extracted, and bacterial near full-length 16S rRNA genes were subsequently amplified, cloned, RFLP-screened, and sequenced. A total of 420 positive clones were classified by RFLP and near-full-length 16S rDNA sequences. Approximately 48 operational taxonomic units (OTUs) were found among 139 positive clones from the superstratum sample; 26 among 149 were from the middle-level sample and 35 among 132 were from the substrate sample. Thermobifida fusca was common in the superstratum layer of the pile. Some Bacillus spp. were remarkable in the middle-level layer, and Clostridium sp. was dominant in the substrate layer. Among 109 OTUs, 99 displayed homology with those in the GenBank database. Ten OTUs were not closely related to any known species. The superstratum sample had the highest microbial diversity, and different and distinct bacterial communities were detected in the three different layers. This study demonstrated the spatial characteristics of the microbial community distribution in the cooling stage of swine manure compost.
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Guo, Yan,Zhang, Jinliang,Yan, Yongfeng,Wu, Jian,Zhu, Nengwu,Deng, Changyan Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.6
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and subsequent sub-cloning and sequencing were used in this study to analyze the molecular phylogenetic diversity and spatial distribution of bacterial communities in different spatial locations during the cooling stage of composted swine manure. Total microbial DNA was extracted, and bacterial near full-length 16S rRNA genes were subsequently amplified, cloned, RFLP-screened, and sequenced. A total of 420 positive clones were classified by RFLP and near-full-length 16S rDNA sequences. Approximately 48 operational taxonomic units (OTUs) were found among 139 positive clones from the superstratum sample; 26 among 149 were from the middle-level sample and 35 among 132 were from the substrate sample. Thermobifida fusca was common in the superstratum layer of the pile. Some Bacillus spp. were remarkable in the middle-level layer, and Clostridium sp. was dominant in the substrate layer. Among 109 OTUs, 99 displayed homology with those in the GenBank database. Ten OTUs were not closely related to any known species. The superstratum sample had the highest microbial diversity, and different and distinct bacterial communities were detected in the three different layers. This study demonstrated the spatial characteristics of the microbial community distribution in the cooling stage of swine manure compost.
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Inhibition of acetylation of histones 3 and 4 attenuates aortic valve calcification
Jia Gu,Yan Lu,Menqing Deng,Ming Qiu,Yunfan Tian,Yue Ji,Pengyu Zong,Yongfeng Shao,Rui Zheng,Bin Zhou,Xiangqing Kong,Wei Sun 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-
Aortic valve calcification develops in patients with chronic kidney disease who have calcium and phosphate metabolic disorders and poor prognoses. There is no effective treatment except valve replacement. However, metabolic disorders put patients at high risk for surgery. Increased acetylation of histones 3 and 4 is present in interstitial cells from human calcific aortic valves, but whether it is involved in aortic valve calcification has not been studied. In this study, we found that treating cultured porcine aortic valve interstitial cells with a high-calcium/high-phosphate medium induced calcium deposition, apoptosis, and expression of osteogenic marker genes, producing a phenotype resembling valve calcification in vivo. These phenotypic changes were attenuated by the histone acetyltransferase inhibitor C646. C646 treatment increased the levels of class I histone deacetylase members and decreased the acetylation of histones 3 and 4 induced by the high-calcium/high-phosphate treatment. Conversely, the histone deacetylase inhibitor suberoylanilide hydroxamic acid promoted valve interstitial cell calcification. In a mouse model of aortic valve calcification induced by adenine and vitamin D treatment, the levels of acetylated histones 3 and 4 were increased in the calcified aortic valves. Treatment of the models with C646 attenuated aortic valve calcification by restoring the levels of acetylated histones 3 and 4. These observations suggest that increased acetylation of histones 3 and 4 is part of the pathogenesis of aortic valve calcification associated with calcium and phosphate metabolic disorders. Targeting acetylated histones 3 and 4 may be a potential therapy for inoperable aortic valve calcification in chronic kidney disease patients.
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Yankai Wu,Xiaolong Qiao,Xinbao Yu,Jiali Yu,Yongfeng Deng 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.2
Expansive soil is considered to be an unfavorable soil due to its swelling-shrinking behavior. In order to improve the properties of expansive soil, the addition of steel slag powder (SSP) has been used to improve expansive soil that has been mixed with the cement. In this study, a series of cylindrical improved expansive soil specimens were prepared, which were improved either by the addition of cement, cement SSP, or cement SSP sodium hydroxide (NaOH). All of the specimens were prepared with an optimum water content and then subjected to a maximum of 12 closed-system freeze-thaw (F-T) cycles. The specimens were subjected to different curing times and temperatures (-5oC,-10oC and -15oC) during the tests. After each freeze-thaw (F-T) cycle, the volume of each specimen was measured and an unconfined compression strength (UCS) test was performed. The results have shown that as the temperature of the F-T cycle decreased, the volume expansion rate increased with the increase of the length of the F-T cycle. As the curing time increased, the effect of the F-T cycles on the volume change rate of the specimens reduced and the UCS increased. The first F-T cycle had the greatest influence on the volume of the specimen as well as the UCS of the improved expansive soil. After the improved expansive soils had undergone more than eight F-T cycles, the volume change rate of the specimen tended to stabilize. The maximum F-T volume change rate of the improved soil was 1.93%.When the curing age was 60d and 90d, the strength of the specimen with cement SSP sodium hydroxide was 377.3 kPa and 294.7 kPa higher than the specimen with cement only (ES specimen), and its strength degradation rate was 18.737% and 9.97% lower than the ES specimen. The results have shown that the addition of SSP and cement improved the expansive soil; moreover, NaOH inhibited the degradation of the soil during an F-T cycle.
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