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Chemical Composition and Cold Flow Property of Cottonseed Oil Biodiesel Fuel
Yongbin Lai,Junfeng Shu,Xiu Chen,Yinnan Yuan,Lei Zhong,Yuqi Zhang,Menghong Yuan,Bo Wang,Pen Wang 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.2
The Cottonseed oil methyl ester (COME) was prepared using an alkali - catalyzed trans esterification reaction, and its chemical composition and cold flow properties (CFP) were studied. Two approaches, viz. blending with petrodiesel and treating with cold flow improver (CFI) were used to improve the CFP of COME. The results showed that COME was mainly composed of fatty acid methyl esters (FAMEs): C14:0–C22:0, C16:1–C20:1, C18:2 and C18:3–C20:3. The mass fractions of saturated and unsaturated FAMEs were 27.69% and 71.65%, respectively. The cold filter plugging point (CFPP) and kinematic viscosity (KV) at 40 °C of COME are -1 °C and 4.63 mm2/s respectively. Blending with -10 petrodiesel (-10PD) and 0 petrodiesel (0PD) can decrease the CFPP of COME/-10PD (B30-B40) and COME/0PD (B40-B50) -12 and -8 °C respectively. With the increase in petrodiesel content, the KV at 40 °C of blending oil decreased, and viscosity-temperature characteristics of blending oil were improved. When used Flow Fit (≤3vol.%), the CFPPs were reduce from COME/- 10PD B5, B7, B10, B20, B50 and COME -8, -8, -9, -11, -11 and -1 °C to -28, -27, -26, -25, -16 and -5 °C respectively; COME/0PD -3, -3, -4, -5, -8 and -1 °C to - 26, -25, -24, -24, -16 and -5 °C respectively.
Study on Volatility of Palm Oil Biodiesel/-10 Petrodiesel by Thermogravimetric Analysis Technique
Yongbin Lai,Peng Wang,Xiu Chen,Yinnan Yuan,Junfen Rong,Guang Wu,Yijun Zhou,Lei Zhong,Yuqi Zhang 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.2
Palm methyl ester (PME) was prepared from palm oil through transesterification using NaOH as catalyst. Chemical composition of the PME and -10 petrodiesel (-10PD) was determined by gas chromatography-mass spectrometer (GC-MS). The PME and -10PD were characterized for their fuel properties including density, kinematic viscosity, and flash point, cold filter plugging point, sulfur content, copper strip corrosion and oxidative stability. Volatility was measured by thermogravimetric analysis (TGA). Volatile index was proposed to describe PME/-10PD volatility. A good correlation model was put forward for calculating the PME/-10PD volatility by PME blending ratio. The study showed that PME was mainly composed of long chain fatty acid methyl esters (FAMEs): C14:0-C24:0, C16:1-C22:1, C18:2 and C18:3. -10PD was mainly composed of long chain alkanes: C8-C26. The fuel properties of PME were within the limits prescribed in the GB/T 20828-2007 standards for biodiesel. With respect to -10PD, volatilization of PME was stronger and quicker, but volatilization onset at higher temperature. The volatilization onset temperatures of PME and -10PD were 448.9 and 361.7 K respectively; and the volatile indexes were 1.76E-04 and 3.64E-05 respectively. The PME/-10PD volatility had relation to PME blending ratio. The volatility of B0-B20 was very close to the -10PD. The volatility of B20-B100 was better with increasing the PME blending ratio.
Shanru He,Chenyuan Yuan,Xue Bai,Tingting Bu,Jie Zhang,Lulu Wang,Chunshan Quan,Yongbin Xu 한국구조생물학회 2022 Biodesign Vol.10 No.2
Anaerobic gram-negative bacterium Fusobacterium nucleatum (F. nucleatum) has long been found to cause opportunistic infections and has recently been implicated in colorectal cancer. In F. nucleatum, YggS (FnYggS) is an important part of bacterial cell walls and belongs to the COG0325 gene family. In this study, YggS from FnYggS was successfully expressed and purified using Ni-NTA affinity and gel-filtration chromatography. The protein crystal was obtained and diffracted to a resolution of 2.08 Å. The preliminary crystallographic analysis suggested that FnYggS crystal belongs to the monoclinic space group P21 with a = 37.93 Å, b = 146.38 Å, and c = 74.13 Å, α = γ = 90.00° and β = 93.36°. The asymmetric unit contained approximately three monomer of FnYggS, giving a crystal volume per mass (VM) of 2.64 Å3 Da–1 and a solvent content of 53.50%.