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Shanru He,Chenyuan Yuan,Xue Bai,Tingting Bu,Jie Zhang,Lulu Wang,Chunshan Quan,Yongbin Xu 한국구조생물학회 2022 Biodesign Vol.10 No.2
Anaerobic gram-negative bacterium Fusobacterium nucleatum (F. nucleatum) has long been found to cause opportunistic infections and has recently been implicated in colorectal cancer. In F. nucleatum, YggS (FnYggS) is an important part of bacterial cell walls and belongs to the COG0325 gene family. In this study, YggS from FnYggS was successfully expressed and purified using Ni-NTA affinity and gel-filtration chromatography. The protein crystal was obtained and diffracted to a resolution of 2.08 Å. The preliminary crystallographic analysis suggested that FnYggS crystal belongs to the monoclinic space group P21 with a = 37.93 Å, b = 146.38 Å, and c = 74.13 Å, α = γ = 90.00° and β = 93.36°. The asymmetric unit contained approximately three monomer of FnYggS, giving a crystal volume per mass (VM) of 2.64 Å3 Da–1 and a solvent content of 53.50%.
Numerical Simulation of Cold Compaction of 3D Granular Packings
Chen Yuan,Imbault Didier,Doremus Pierre 한국분말야금학회 2006 한국분말야금학회 학술대회논문집 Vol.2006 No.1
During cold compaction processes loose powder is pressed under tooling action in order to produce complex shaped engineering components. Here, the analysis of the plastic deformation of granular packings is of fundamental importance to the development of computer simulation models. Powders can be idealized by packing discrete particles, where each particle is a sphere meshed with finite elements. The pressing of a body centered cubic packing was compared with numerical prediction and experimental data. The global response was expressed in force-displacement curve, and the accuracy of the numerical models analyzed for high relative densities up to 0.95.