1996-1997년 겨울철에 유행한 인플루엔자의 임상역학적 분석 및 원인 바이러스의 분리

김수정,정규영,이용화,기창석,이남용,이상일,김지희 대한감염학회 1997 감염 Vol.29 No.4

목적: 인플루엔자는 매년 겨울 전 세계적인 유행을 일으키는 급성 호흡기 질환으로 높은 이환률과 사망율을 보인다. 국내에서도 1996-1997년 겨울철에 다수의 보고가 있었으나 아직 체계적인 감시가 이루어지고 있지 못하다. 저자들은 이 기간 동안 인플루엔자 바이러스 감염의 역학적 특성과 임상상을 연구하여 인플루엔자 감시의 기초 자료를 제공하고자 하였다. 방법: 1996년 10월부터 1997년 4월까지 삼성서울병원 임상병리과에 인플루엔자 바이러스 배양 검사가 의뢰된 모든 검체를 대상으로 인플루엔자 바이러스의 분리율과 유행 양상, 그리고 환자들의 임상상을 후향적으로 조사, 분석하였다. 결과: 총 461명의 환자로부터 525례의 검체가 의뢰되어 총 98명의 환자로부터 인플루엔자 바이러스가 분리되었다. 인플루엔자 A형은 54명의 소아 환자와 4명의 소아 환자로부터 분리되었다. 아형과 항원 특성 검사를 시행한 31례의 인플루엔자 A형 중 한 예에서 A/Wuhan/359/95 유사주로 확인되었고 12례의 인플루엔자 B형 중 5례에서 B/Guangdong/8/97 유사주로 확인되었다. 인플루엔자의 유행 양상은 3월과 4월 중순, 두 번의 인플루엔자 최고 분리 시점이 관찰되었고, 가장 많은 불리 빈도를 보인 연령층은 인플루엔자 A형의 경우 1세 미만이었고 인플루엔자 B형의 경우 3-5세였으며 폐렴이 가장 흔한 호흡기 질환이었다. 결론: 저자들은 3차 의료기관에서 분리된 인플루엔자 바이러스에 대한 역학적, 임상적 특성을 연구하였다. 지역사회에 대한 광범위한 연구가 아니므로 국내의 전반적 상황과는 차이가 있을 것으로 생각되지만 우리나라에서도 인플루엔자 바이러스의 이환률이 낮지 않을 것으로 생각되며, 보다 조직적이고 전국적인 인플루엔자 감시가 이루어져야 할 것으로 사료된다. Background: Although influenza has been a leading cause of global morbidity and mortality, we have few data regarding the epidemioloical and clinical characteristics of influenza activity in korea. Since an outbreak of influenza was recognized during winter of 1996-1997, we analysed the epidemiological and clinical features of influenza activity in the hospital seeting. Methods: All clinical specimens requested for isolation of influenza virus at Samsung Medical Center from October 1996 to April 1997 were included. Mardin-Darby canine kidney (MDCK) cell line was used for virus culture. Isolated viruses were confirmed with immunostain followed by subtyping. The demograpic and clinical characteristics of the patients were reviewed retrospectively. Results: Ninety eight influenza viruses were isolatd from 461 patients (21.3%). Influenza A and B virus were isolated from 58 (54 children and 4 adults) and 40 pediatric patients, respectively. One of 31 influenza A viruses was confirmed as A/Wuhan/359/95-like strain and 5 of 12 influenza B viruses were confiremd as B/Guangdong/8/97-like strains. Two distinctive peaks of influenza activity were recognized and the most common age of patients was less than 1 year for influenza A, and 3 to 5 years for influenza B. Common lower respiratory infections were pneumonia followed by croup, bronchiolitis and laryngitis. Conclusion: We analyzed the epidemiological and clinical features of influenza activity during winter of 1996-1997. Although this study was performed not in the community but in the hospital setting, the morbidity caused by influenza may not be low in Korea. Therefore, nationwide surveillance for influenza activity is warranted.

CALS의 구현 전략에 관한 연구

李庸和 忠州大學校 1998 한국교통대학교 논문집 Vol.33 No.1

The sudden change of management environment forces to enterprise seeking to various plans. Among these various plans, Information technology is used to deal with the changing management environment. At this point of view, there are continuous efforts for adopting, the military's CALS refer to about the concept, development process emboding goal, core strategic point and emboding strategy of CALS.

합성 펩티드를 이용한 Interleukin-2수용체 β-Chain에 대한 단일클론항체의 제작

이희구,이홍수,이인애,최용경,최인성,정태화,김길현 梨花女子大學校 藥學硏究所 1995 藥學硏究論文集 Vol.- No.5

Hybridoma producing monoclonal antibodies reactive with the ß-chain of interleukin-2 receptor (IL-2R) were generated from mice immunized with a synthetic pepride of 19 amino acids. The synthetic peptide constitutes strongly hydrophilic region of extracellular domain of the IL-2R molecules(residues 32-50) as revealed by hydrophathy plot analysis. Hybridomas were screened by ELISA using Hut102 cells and the synthetic peptide as antigenic moiety, and by measuring the binding activity of antibodies to the Hut102 cell surface that was monitored by a flow cytometer. The antibodies were further screened by their inhibitory effect against the interaction between IL-2R and interleukin-2(IL-2). Antibodies were further characterized by immunoprecipitation followed by autoradiography to reveal that the antibodies recongnize the 75 KD protein molecules expressed on the Hut 102 cell surface and human peripheral blood mononuclear cells(PBMC) which confirms the antigenic moiety to be ß-chain of IL-2 recepter molecules.

정보기술이 기업조직의 변화에 미치는 영향에 관한 연구

李庸和 충주대 2000 한국교통대학교 논문집 Vol.35 No.1

The change of management environment by application of information technology give to enterprise both threat and opportunity.It is require to enterprise establishing new management goal and strategy.Therefore the management organization and process have to restructured for matching the changing management environment, At this point, this research described about the impact application of information technology to the management organization.

전자 상거래의 성공요인에 관한 연구

李庸和 충주대 2001 한국교통대학교 논문집 Vol.36 No.1

With the development of Internet technology, the use of open global communication network used as a Business Purpose.The technology and system development of internet move the traditional offline commerce into online and which create new business area called Electronic Commerce. Electronic Commerce become a new business model that can be overcome the restriction of time and space.Thus many country in the world promote Electronic Commerce as a tool of strengthening national power. At this paper, study the basic understanding and sucessful factor of electronic commerce

페파 크로마토그래피에 의한 各種 果實중의 糖類 分析

李用華 春川敎育大學 1983 論文集 Vol.23 No.-

1. Paper chromatography에 의한 各種 果實들 中에는 살구에는 Fructose가 없고 매실에는 Glucose가 없는 外에 모든 果實에는 Glucose와 Fructose는 모두 含有되어 있다. 2. Sucrose는 산딸기, 참외, 수박, 오디, 도마도, 버치, 앵두, 매실, 포도(赤), 귤등에는 없고 사과, 머루, 배, 포도, 복숭아, 바나나, 메론 등에 나타났다. 卽 작은 열매에는 含有되어 있지 않으나 나무에서 생성되는 果實들은 大體로 Sucrose가 含有되어 있다. 3. Xylose는 新鮮하게 處理된 試料에서만 나타났다. 사과, 복숭아, 머루, 포도, 감등에서 나타났으며 다른 열매 등에서는 나타나지 않았다. Aniline Oxalate매 의하여 Pink color로 發色되었다가 곤 消失되므로 確認하는데 좀 難點이 있다. 4. 모든 果實 中에도 머루와 靑포도에는 Glucose, Fructose, Arabinose 外에도 더욱 몇개의 많은 糖類들이 含有되어 있다.

합성 펩티드를 이용한 Interleukin-2수용체 β-Chain에 대한 단일클론항체의 제작

이희구,이홍수,이인애,최용경,최인성,정태화,김길현 이화여자대학교 생명과학연구소 1995 생명과학연구논문집 Vol.6 No.-

Hybridoma producing monoclonal antibodies reactive with the β-chain of interleukin-2 receptor (IL-2R) were generated from mice immunized with a synthetic peptide of 19 amino acids. The synthetic peptide constitutes strongly hydrophilic region of extracellular domain of the IL-2R molecules(residues 32-50) as revealed by hydrophathy plot analysis. Hybridomas were screened by ELISA using Hut102 cells and the synthetic peptide as antigenic moiety, and by miasuring the binding activity of antivodies to the Hut102 cell surface that was monitored by a flow cytometer. The antibodies were further screened by their inhibitory effect against the interaction between IL-2R and interleukin-2(IL-2). Antibodies were further characterized by immunoprecipitation followed by autoradiography to reveal that the antibodies recongnize the 75 KD protein molecules expressed on the Hut102 cell surface and human peripheral blood mononuclear cells(PBMC) shich confirms the antigenic moiety to be β-chain of IL-2 recepter molecules.

지식경영의 성공전략에 관한 연구

이용화 忠州大學校 2002 한국교통대학교 논문집 Vol.37 No.4

OECD and International Bank for Reconstruction and Development(IBRD) anounced at the report of world development which related to the knowledge management, the new paradigm of instituting industrial society is knowledge based economy.

Game 理論을 이용한 廣告費의 最適配分에 관한 硏究

李庸和 忠州大學校 1987 한국교통대학교 논문집 Vol.20 No.2

An investgation item by the event of advertisement activity planning is as follows : 1) The Model of advertisement cost distribution in the region by n-unit advertisement activity. 2) The Model of adverisement cost in the advertisement time by n-time. In this paper we try to find a mathematics solution to the problems by game theory.

지식경영의 성공요인에 관한 연구

이용화 忠州大學校 2003 한국교통대학교 논문집 Vol.38 No.4

The Knowledge Management, starting with the concept of efficient management and control of core knowledge can affect the enterprise's outcome, become conspicuous as a new management technique. Such a Knowledge Management more focus on the necessity with the rapid development of Information Technology and globalization of management environment accompany with the reducing life-cycle of product and service and the diversification of customer needs. On this study, define the concept and range of Knowledge Management, and the basic factors which need to build systematic frame of the Knowledge Management.