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Yin Xi,Min Xiangde,Nan Yan,Feng Zhaoyan,Li Basen,Cai Wei,Xi Xiaoqing,Wang Liang 대한영상의학회 2020 Korean Journal of Radiology Vol.21 No.8
Objective: To compare the accuracies of quantitative computed tomography (CT) parameters and semiquantitative visual score in evaluating clinical classification of severity of coronavirus disease (COVID-19). Materials and Methods: We retrospectively enrolled 187 patients with COVID-19 treated at Tongji Hospital of Tongji Medical College from February 15, 2020, to February 29, 2020. Demographic data, imaging characteristics, and clinical data were collected, and based on the clinical classification of severity, patients were divided into groups 1 (mild) and 2 (severe/ critical). A semiquantitative visual score was used to estimate the lesion extent. A three-dimensional slicer was used to precisely quantify the volume and CT value of the lung and lesions. Correlation coefficients of the quantitative CT parameters, semiquantitative visual score, and clinical classification were calculated using Spearman’s correlation. A receiver operating characteristic curve was used to compare the accuracies of quantitative and semi-quantitative methods. Results: There were 59 patients in group 1 and 128 patients in group 2. The mean age and sex distribution of the two groups were not significantly different. The lesions were primarily located in the subpleural area. Compared to group 1, group 2 had larger values for all volume-dependent parameters (p < 0.001). The percentage of lesions had the strongest correlation with disease severity with a correlation coefficient of 0.495. In comparison, the correlation coefficient of semiquantitative score was 0.349. To classify the severity of COVID-19, area under the curve of the percentage of lesions was the highest (0.807; 95% confidence interval, 0.744–0.861: p < 0.001) and that of the quantitative CT parameters was significantly higher than that of the semiquantitative visual score (p = 0.001). Conclusion: The classification accuracy of quantitative CT parameters was significantly superior to that of semiquantitative visual score in terms of evaluating the severity of COVID-19.
Xi-Jun Yin,Hyo-Sang Lee,양철주,배인휴,오동환,Seong-Gyun Cho,공일근 아세아·태평양축산학회 2007 Animal Bioscience Vol.20 No.5
This study was carried out to evaluate the effect of artificial insemination (AI) time and methods on fertilization and delivery rate at onset of estrus cats. Artificial insemination method was used by a transcervical insemination (TCI) or intra-vaginal insemination (IVI) using frozen epididymal sperm (FES) with Norwegian catheter. Semen was collected from epididymal spermatozoa after testis ectomization and frozen in Tris-buffered solution supplemented with 7% glycerol and 0.5% Orvus ES Paste. The concentration of frozen spermatozoa was adjusted to 200106 sperm/ml. The CASA data on motility and progressive motility of FES after thawing was approximately 40.35.8 and 35.96.5%. The female cats were subcutaneously treated with 50 IU pregnant mare serum gonadotropin (PMSG) and ovulated with 100 IU human chorionic gonadotropin (hCG) 4 days after PMSG injection. One treatment was inseminated at 36 h after hCG injection and the other treatment was inseminated immediately hCG injection. At 36 h after hCG injection group, 5 female cats were inseminated TCI or IVI method using 50106 sperm/0.2 ml per AI. Three of 5 female cats could be inseminated by TCI method and then delivered the 10 kittens from 2 of 3 inseminated females. Immediately hCG injection group, 4 female cats were inseminated TCI or IVI method using same sperm concentration per AI. Two of 4 female cats could be inseminated by TCI method and then delivered the 4 kittens from 1 of 2 inseminated female cats. The results revealed that AI time and method could be affected the fertilization and delivery rates at onset of estrus cats.
Generation of Cloned Transgenic Cats Expressing Red Fluorescence Protein1
Yin, Xi Jun,Lee, Hyo Sang,Yu, Xian Feng,Choi, Eugene,Koo, Bon Chul,Kwon, Mo Sun,Lee, Young S.,Cho, Su Jin,Jin, Guang Zhen,Kim, Lyoung Hyo,Shin, Hyoung Doo,Kim, Teoan,Kim, Nam Hyung,Kong, Il Keun Oxford University Press 2008 BIOLOGY OF REPRODUCTION Vol.78 No.3
<P>A method for engineering and producing genetically modified cats is important for generating biomedical models of human diseases. Here we describe the use of somatic cell nuclear transfer to produce cloned transgenic cats that systemically express red fluorescent protein. Immature oocytes were collected from superovulating cat ovaries. Donor fibroblasts were obtained from an ear skin biopsy of a white male Turkish Angora cat, cultured for one to two passages, and subjected to transduction with a retrovirus vector designed to transfer and express the red fluorescent protein (RFP) gene. A total of 176 RFP cloned embryos were transferred into 11 surrogate mothers (mean = 16 +/- 7.5 per recipient). Three surrogate mothers were successfully impregnated (27.3%) and delivered two liveborn and one stillborn kitten at 65 to 66 days of gestation. Analysis of nine feline-specific microsatellite loci confirmed that the cloned cats were genetically identical to the donor cat. Presence of the RFP gene in the transgenic cat genome was confirmed by PCR and Southern blot analyses. Whole-body red fluorescence was detected 60 days after birth in the liveborn transgenic (TG) cat but not in the surrogate mother cat. Red fluorescence was detected in tissue samples, including hair, muscle, brain, heart, liver, kidney, spleen, bronchus, lung, stomach, intestine, tongue, and even excrement of the stillborn TG cat. These results suggest that this nuclear transfer procedure using genetically modified somatic cells could be useful for the efficient production of transgenic cats.</P>