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- 저자 : Yilan Chen (검색결과 4 건)
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Ruonan Chen,Yilan Liu,Wei Zhong,Hao Xuemi,Tingzhen Mu,Maohua Yang,Jianmin Xing 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.4
Fatty alcohols are widely used in the fields of food, pharmaceutical and renewable energy and so on, thus biosynthesis of fatty alcohols has attracted wide attention in recent years. However, the concentration of fatty alcohols is still at a low level. In this paper, we constructed an Escherichia coli strain with high yield of fatty alcohols through the combinatorial regulation of three key genes of fabH, fabZ, and far. Firstly, we designed new ribosomebinding sequences (RBS) of fabH and fabZ with 10-fold strengths compared to the original ones by RBS calculator, separately. Secondly, we engineered the starting strain MGL1 by replacing the original RBS with designed ones, which resulted in MGLHS (fabH-RBS1), MGLZS (fabZRBS2), and MGLHZS (fabH-RBS1/fabZ-RBS2), respectively. Thirdly, the plasmid pL1 harboring far gene was transferred into above strains to test fatty alcohols production under the induction of different isopropyl-β-D-thiogalactoside concentrations. The results showed that the concentration of fatty alcohols in strains MGLHS-far, MGLZS-far and MGLHZS-far reached 2.75 g/L, 2.54 g/L and 3.82 g/L, respectively, which increased 35%, 25% and 88% than that of the starting strain MGL1-far. The fatty alcohols reached 9.35 g/L in fed batch fermentation with MGLHZS-far, of which the main components were two saturated fatty alcohols (C14:0 and C16:0) and two unsaturated fatty alcohols (C16:1 and C18:1). The results demonstrated that combinatorial regulation of three key genes of fabH, fabZ, and far in E. coli can achieve high-yield production of fatty alcohols.
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Xiaona Wang,Haiyuan Zhao,Yilan Shao,Pei Wang,Yanru Wei,Weiqian Zhang,Jing Jiang,Yan Chen,Zhigang Zhang 한국영양학회 2014 Nutrition Research and Practice Vol.8 No.1
Inorganic arsenic (iAs) is a toxic metalloid found ubiquitously in the environment. In humans, exposure to iAs can result in toxicity and cause toxicological manifestations. Arsenic trioxide (As2O3) has been used in the treatment for acute promyelocytic leukemia. The kidney is the critical target organ of trivalent inorganic As (iAs<SUP>Ⅲ</SUP>) toxicity. We examine if oral administration of astaxanthin (AST) has protective effects on nephrotoxicity and oxidative stress induced by As2O3 exposure (via intraperitoneal injection) in rats. Markers of renal function, histopathological changes, Na<SUP>+</SUP>-K<SUP>+</SUP> ATPase, sulfydryl, oxidative stress, and As accumulation in kidneys were evaluated as indicators of As2O3 exposure. AST showed a significant protective effect against As2O3-induced nephrotoxicity. These results suggest that the mechanisms of action, by which AST reduces nephrotoxicity, may include antioxidant protection against oxidative injury and reduction of As accumulation. These findings might be of therapeutic benefit in humans or animals suffering from exposure to iAs<SUP>Ⅲ</SUP> from natural sources or cancer therapy.
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Lin Xiaoying,Zeng Weipeng,Chen Yilan,Su Ting,Zhong Qinhua,Gong Linzhu,Liu Yamin 한국탄소학회 2022 Carbon Letters Vol.32 No.3
A porous-carbon material UiO-66-C was prepared from metal–organic frameworks UiO-66 by carbonization in inert gas atmosphere. Physicochemical properties of UiO-66-C materials were well characterized by Powder X-ray diffraction (PXRD), Scanning electron microscope (SEM), Fourier-transform infrared spectroscopy (FT-IR), Raman spectrometer, N2 adsorption/desorption isotherms (BET), and the adsorption properties of the products were studied UiO-66-C has a high specific surface area up to 1974.17 m2/g. Besides, the adsorption capacity of tetracycline could reach 678.19 mg/g, the adsorption processes agreed well with the pseudo-second-order kinetic model and Langmuir isotherm model.
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Lijun Du,Ning Lv,Jiashuai Li,Jiayi Zhang,Yilan Chen,Yalin Zhang,Zhen Li,Xianqiang Huang,Jujie Luo 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.120 No.-
NiCoSe4 nanoflowers were synthesized on carbon fiber felt (CFF) by microwave method, and the effects ofmicrowave time and microwave power on the morphology, physical phase and performance ofNiCoSe4@CFF were explored. The results display that the nanostructured NiCoSe4 prepared under themicrowave irradiation of 1000 W for 120 s is uniformly loaded on the CFF. The NiCoSe4@CFF electrodeexhibits a superior specific capacity of 1653.6F g1 at 1A/g and maintains a superior cycling performanceof 87.05 % of the initial capacitance over 150,000 cycles. In addition, the flexible supercapacitor fabricatedwith NiCoSe4@CFF as the cathode exhibits excellent flexibility and flexural strength. As an effective catalystfor oxygen evolution reaction (OER), NiCoSe4@CFF demonstrates a low overpotential of 0.358 V, arelatively small Tafel slope of 115.93 mV dec1 and an excellent lifetime at 10 mA cm2.
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