Comparison of stomach microRNA transcriptomes of Tibetan and Yorkshire pigs by deep sequencing

Wen‑kui Sun,Yanyue Li,Chi Cheng,Yi‑hui Chen,Kai Zeng,Xiaohui Chen,Yiren Gu,Rui Liu,Xuebin Lv,Rong Gao 한국유전학회 2018 Genes & Genomics Vol.40 No.9

MiRNAs regulate the expression of target genes in diverse cellular processes and hence play important roles in different physiological processes, yet little is known about the stomach microRNAome (miRNAome) of the Tibetan pig. The objective of this experiment was to investigate differentially expressed stomach miRNAs participating in digestion. Firstly, we isolated total RNA by Trizol reagent from three Tibetan and three Yorkshire purebred pigs stomach samples at 90-day-old. Secondly, a comprehensive analysis of Tibetan and Yorkshire pig stomach miRNAomes was performed by small RNA sequencing in the Illumina HiSeq 2000 system. Finally, SYBR Green Real-time RT-PCR was performed to validate the differentially expressed miRNAs. We identified 318 unique miRNAs, 260 were co-expressed in both libraries, 17 and 31 miRNAs were specifically expressed in Tibetan and Yorkshire pigs respectively. Fifty six differentially expressed miRNAs were identified by the identifying differentially expressed genes 6 (IDEG6). Kyoto encyclopedia of genes and genomes analysis revealed that some of the differentially expressed miRNAs were associated with protein and fat digestion. Two differentially expressed miRNAs (miR-214-3p and ssc-un39) participating in the digestion of lipid were identified. Additionally, qRT-PCR results suggested that a higher expression of miR-214-3p in the Tibetan pig stomach could lead to relatively lower expression of calcium-dependent phospholipase A2, which is an enzyme important for the digestion of glycerol phospholipid. This study has delineated the different stomach miRNAs expression patterns of Tibetan and Yorkshire pigs, which would help explain the regulatory mechanisms of miRNAs in digestion of Tibetan pigs, and contribute to utilize a the unique digestion merits of Tibetan pig in future porcine hybridization breeding.

Broad-specificity amino acid racemase, a novel non-antibiotic selectable marker for transgenic plants

Yi‑Chia Kuan,Venkatesan Thiruvengadam,Jia‑Shin Lin,Jia‑Hsin Liu,Tsan‑Jan Chen,Hsin‑Mao Wu,Wen‑Ching Wang,Liang‑Jwu Chen 한국식물생명공학회 2018 Plant biotechnology reports Vol.12 No.1

The broad-specificity amino acid racemase (Bsar) from Pseudomonas putida catalyzes the racemization of various amino acids, offering a flexible and feasible platform to develop a new non-antibiotic selectable marker system for plant transformation. In the present study, we demonstrated that a Bsar variant, Bsar-R174K, that is useful as a selectable marker gene in Arabidopsis and rice that were susceptible to l-lysine and D-alanine. The introduction of wild-type Bsar, Bsar-R174K or Bsar-R174A into E. coli lysine or asparagine auxotrophs was able to rescue the growth of these microorganisms in minimal media supplemented with selectable amino acid enantiomers. The transformation of Arabidopsis with Bsar or Bsar variants based on d-alanine selection revealed that Bsar-R174K had the greatest efficiency (2.40%), superior to kanamycin selectionbased transformation (1.10%). Whereas, l-lysine-based selection exhibited lower efficiency for Bsar-R174K (0.17%). The progenies of selected Bsar-R174K transgenic Arabidopsis revealed normal growth properties. In addition, Bsar-R174K transgenic rice was obtained on l-lysine medium with an efficiency of 0.9%, and the progenies of the transgenic rice revealed morphologically normal phenotypes comparable with their wild-type counterparts. This study presents the first report of broad range amino acid racemase Bsar-R174K as a non-antibiotic selectable marker system applied in transgenic plants.

Research on Current Execution of Customer Support Knowledge Management System of Medical Appliances Industry

( Yi Chan Chung ),( Chih Hung Tsai ),( Shiaw Wen Tien ),( Lin Yi Lin ) 한국품질경영학회 2007 The Asian Journal on Quality Vol.8 No.3

Color Harmony on 3D Color Configuration

Wen-Yuan Lee,Sin-Jhe Huang,Yi-Wen Chang 한국색채학회 2011 한국색채학회 학술대회 Vol.2011 No.10

The purpose of this study is to see the "harmonious- disharmonious" feeling of color combination on 3D color configuration. A cuboid with side circle shape was used to display 122 color combinations. Each color combination was evaluated on "harmonious-disharmonious" scale by 32 observers. The results showed that color harmony for the color combinations having an achromatic color differed from those having two chromatic colors. For the color combinations having an achromatic color, the color harmony was evoked by sum of lightness. The most disharmonious feeling was evoked when the sum of lightness equal to 85. When both neighboring colors are chromatic colors, color harmony was determined by hue angle difference. Two colors having similar hue appeared harmonious feeling.

중국 윈난성 뎬츠(滇池)보호에서의 공중참여에 관한 연구 : 자원동원이론의 시각으로

Yi-Hua Wen,Yue Sun,Ming Liu 한국환경사회학회 2019 환경사회학연구 ECO Vol.23 No.2

This research takes the private environment organization--Green Kunming as an example, to explore the growth of resources by mobilization. The study includes a public participation activity--Protecting Lake Denz, the largest lake in Kunming, Yunnan Province. To ensure this organization survive and thrive, we must have sufficient resources and are able to mobilize them. This study focuses on the development of Green Kunming ancestry conservation activities based on institutional resources, financial resources, human resources and social resources. These resources are organically integrated with each other and are characterized by collective actions. The survey results show that Green Kunming is limited by institutional resources, but this is also a prerequisite for obtaining legitimacy and obtaining new resources. Financial resources are mainly funded by private fund meetings. and these funds are a major participatory survival method including projects. In mobilizing human resources, the members are mainly volunteers. Doing this will ensure the sustainability of group development, promote the formation of social capital, and fully mobilize new media as a social resource. This study aims to determine the growth of citizens in our society, and hope to use it as an indicator to measure the degree of the political structure openness to the Chinese public participation and the degree of democratization in the public sphere. 본 연구는 중국 윈난성 쿤밍시에서 가장 큰 호수인 뎬츠(滇池)에 대한 보호를 비롯한 공중의 참여활동 중에서 민간 환경단체-녹색곤명을 사례로, 자원동원론의 시각으로 참여과정에 자원을 어떻게 동원하면서 성장하였는가를 살펴본다. 단체가 생존하고 성장하려면 다양한 자원을 소유함과 아울러 자원을 충분히 동원하여야 한다. 이런 자원들은 또 서로 유기적으로 결합되면서 단체활동 전개의 특성을 가진다. 본고에서는 자원을 동원하기 위한 전제조건, 재정적 자원, 인적 자원, 사회적 자원을 토대로 뎬츠보호의 주요한 행위주체‒녹색곤명의 뎬츠보호 활동의 전개에 대한 분석이 중심을 이룬다. 연구결과 녹색곤명은 합법적인 지위를 취득하여 새로운 자원을 얻을 수 있는 정치적 기회구조를 확충하고, 재정적 자원은 주로 민간 기금회의 자금 지원을 통해 얻는데 이러한 자금조달은 프로젝트를 비롯한 참여방식이 된다. 인적 자원의 동원을 볼때 구성원은 주로 자원봉사자들인데 이러한 특성은 인력동원이 용이하고 자발적으로 활동할 수 있으며 단체 발전의 지속성을 보장하며, 사회자본의 형성을촉진할 수 있다. 사회적 자원으로는 주로 뉴미디어를 활용했는데 전파속도가빠르고 전파범위가 넓어서 공론장의 형성을 촉진하였다. 이러한 현상을 통해중국에서 공중참여에 대한 제도적 보장은 이미 갖춰져 있고, 정치적 기회 구조는 질적으로 변화하고 있으며, 정부의 집권이념의 변화가 시민사회의 성장을추진하고 있다는 결론을 얻을 수 있었다.

Rheinheimera aquatica sp. nov., Antimicrobial Activity-Producing Bacterium Isolated from Freshwater Culture Pond

( Wen Ming Chen ),( Yi Lin Chang ),( Chung Young Chiu ),( Yi Sheu Shih ) 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.10

A bacterial strain designated GR5T, previously isolated from a freshwater culture pond in Taiwan while screening for bacteria for antimicrobial compounds, was characterized using a polyphasic taxonomic approach. Strain GR5T was found to be Gram-negative, aerobic, greenish-yellow colored, rod-shaped, and motile by means of a single polar flagellum. Growth occurred at 10-40oC (optimum, 35oC), pH 7.0-8.0 (optimum pH 8.0), and with 0-2.0% NaCl (optimum, 0.5-1.0%). The major fatty acids were C16:1ω7c (36.3%), C16:0 (16.6%), C12:0 3-OH (12.5%), and C18:1ω7c (9.1%). The major respiratory quinone was Q-8, and the DNA G+C content of the genomic DNA was 51.9 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain GR5T belongs to the genus Rheinheimera, where its most closely related neighbors are Rheinheimera texasensis A62-14BT and Rheinheimera tangshanensis JA3-B52T with sequence similarities of 98.1% and 97.5%, respectively, and the sequence similarities to any other recognized species within Gammaproteobacteria are less than 96.5%. The mean level of DNA-DNA relatedness between strain GR5 T and R. texasensis A62-14BT, the strain most closely related to the isolate, was 26.5±7.6%. Therefore, based on the phylogenetic and phenotypic data, strain GR5T should be classified as a novel species, for which the name Rheinheimera aquatica sp. nov. is proposed. The type strain is GR5T (=BCRC 80081T= LMG 25379T).

Wave propagation in spherical and cylindrical panels reinforced with carbon nanotubes

Yi-Wen Zhang,Hao-Xuan Ding,Gui-Lin She 국제구조공학회 2023 Steel and Composite Structures, An International J Vol.46 No.1

Based on the third-order shear deformation theory, the wave propagations in doubly curved spherical- and cylindrical- panels reinforced by carbon nanotubes (CNTs) are firstly investigated in present work. The coupled equations of wave propagation for the carbon nanotubes reinforced composite (CNTRC) doubly curved panels are established. Then, combined with the harmonic balance method, the eigenvalue technique is adopted to simulate the velocity-wave number curves of the CNTRC doubly curved panels. In the end, numerical results are showed to discuss the effects of the impact of key parameters including the volume fraction, different shell types (including spherical (R1=R2=R) and cylindrical (R1=R, R2=→∞)), wave number as well as modal number on the sensitivity of elastic waves propagating in CNTRC doubly curved shells.

Fabrication of Photopolymer Hierarchical Micronanostructures by Coupling Electrospinning and Photolithography for SERS Substrates

Wen-Yi Zhang,Xin-Ze Xiao,Chao Lv,Jia Zhao,Gong Wang,Xuan Gu,Ran Zhang,Bin-Bin Xu,Dan-Dan Zhang,Ai-Wu Li,Yong-Lai Zhang,Hong-Bo Sun 한국고분자학회 2013 Macromolecular Research Vol.21 No.3

Reported here is the fabrication of photopolymer hierarchical micronanostructures through a combinative process of electrospinning and subsequent photolithography. Electrospun SU-8 (epoxy-based negative photoresist)nanofiber films have been patterned into gratings with periods of 100, 200, 300, and 400 μm, respectively. Deposition of a silver nanolayer on these interlaced nanofiber films would lead to the formation of various plasmonic nanostructures,and therefore, giving rise to abundant surface-enhanced Raman scattering (SERS) “hot spots”. In the detection of Rhodamine 6G (R6G), probing molecule, the resultant SERS substrates show both high sensitivity and good reproducibility. The SERS enhancement factor could reach as high as ~108, indicating high efficiency. The fabrication of patterned, highly efficient SERS substrates may hold a great promise for the integration of SERS substrates in various microdevices such as microfluidic chips.

PF2405, standardized fraction of Scutellaria baicalensis, ameliorates colitis in vitro and in vivo

Wen-Yi Jiang,서검석,김윤철,손동환,이성희 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.6

Standardized extraction procedures for herb areas important as their authentication to maintain theirquality and ensure their safe use. We had prepared astandardized and purified Scutellaria baicalensis Georgiextract, PF2405, which was enriched with three majorcomponents, baicalein, oroxylin A and wogonin. In thepresent study, we investigated the potential anti-inflammatoryeffects of PF2405 in vitro and in two differentexperimental animal models of inflammatory bowel disease. Effect of PF2405 studied in tumor necrosis factor(TNF)-a-induced HT-29 cells in vitro. In vivo experimentalcolitis models were induced by administration oftrinitrobenzene sulfonic acid (TNBS) or dextran sulfatesodium (DSS). PF2405 (50 lg/ml) decreased TNF-ainducedcyclooxygenase (COX)-2 expressions throughinhibition of phosphorylation of c-Jun N-terminal kinasesand p38 mitogen-activated protein kinase in HT-29 cells. Combination of baicalein (20 lg/ml), oroxylin A (8 lg/ml),and wogonin (2 lg/ml) markedly inhibits TNF-a-inducedCOX-2 expression when compared with individual components. PF2405 (25 mg/kg b.w.) treatment significantlyreduced histopathological severity; suppressed expression ofCOX-2, TNF-a, and interleukin-1b in TNBS-induced mice. Moreover, PF2405 (25 mg/kg b.w.) has both potent preventiveand therapeutic activities in DSS-induced colitis. Collectively, PF2405 shows prominent anti-inflammatoryeffect that can be used as a new therapeutic approach forintestinal inflammatory disorders.

Antioxidant Activity of Cleomiscosins A and C Isolated from Acer okamotoanum

Wen Yi Jin,Phuong Thien Thuong,Nguyen Duy Su,민병선,손건호,장현욱,김현표,강삼식,석대은,배기환 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.3

Phytochemical investigation of Acer okamotoanum leaf and twig led to the isolation of two coumarinolignans, cleomiscosin A (1) and cleomiscosin C (2). Here, we found that 2 dose-dependently inhibits LDL oxidation mediated by either catalytic copper ions (Cu2+) or free radicals generated with the azo compound 2,2'-azobis-(2-amidinopropane)dihydro-chloride (AAPH) with IC50s of 29.5 and 11.9 µM, respectively. By electrophoretic analysis, we also observed that 2 protects apolipoprotein B-100 (apoB-100) against Cu2+-induced fragmentation (65.3% inhibition at 5 µM). Furthermore, fluorescence analyses clearly indicated that both 1 and 2 protect against the oxidative modification of apoB-100 induced by either Cu2+ or HOCl (1, IC50s of 13.4 and 8.1 µM, respectively; 2, IC50s of 23.6 and 3.9 µM, respectively). These findings suggest that 1 and 2 could be beneficial in preventing LDL oxidation in atherosclerotic lesions.