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Application of genetically engineered Salmonella typhimurium as tumor targeting agents
Yeongjin Hong,Seung-Hwan Park,Seong Young Kwon,Jung-Joon Min 제어로봇시스템학회 2013 제어로봇시스템학회 국제학술대회 논문집 Vol.2013 No.10
Some attenuated bacteria have been studied as viable tumor-targeting agents. Among them, Salmonella typhimurium carrying many advantages such as growth in both of aerobic and anaerobic conditions, various attenuated virulent strains, universal genetic engineering tools, and the clarified full genome sequences has been studied the most up to now. In this paper, we review recent progresses of tumor treatment using this bacterium.
Rare earth metal-doped Zintl phase thermoelectric materials:The Yb5xRExAl2Sb6(RE=Pr, Nd, Sm) system
Yeongjin Hong,Tae-Soo You,Seongbeom Yeon,Jiwon Jeong,Dohyun Moon 대한화학회 2022 Bulletin of the Korean Chemical Society Vol.43 No.10
Three Zintl phase solid solutions in the Yb5xRExAl2Sb6(RE=Pr, Nd, Sm)system have successfully been synthesized by arc melting followed by anneal-ing. The isotypic crystal structure of these compounds was characterized byPXRD and SXRD analyses, and their Ca5Al2Bi6-type structure (space groupPbam,Z=2) was described as a combination of (1) the 3-dimensional anionic[Al2(Sb4Sb4/2)] frameworks and (2) the space-filling cations Yb2+and RE3+located in between the anionic frameworks. In particular, the anionic frame-works were originally built from the two neighboring tetrahedral [AlSb4] moie-ties via the Sb1-Sb1 bridges. The site-preference of RE3+for the Yb3/RE-sitewith the mixed occupation ratio between ca. 9% and 16% of RE3+was eluci-dated by the size-factor criterion based on the size match between the cationicsize and the site volume. The band structures, density of states, and crystalorbital Hamilton population curve analyses were conducted by the tight-binding linear muffin-tin orbital method, and the results proved that the RE3+-doping increased the band degeneracies and the number of resonance peaksnear the Fermi level resulting in the improved Seebeck coefficients. The electri-cal transport property measurements proved that despite the successful addi-tion ofn-type RE3+dopants in the title Yb5xRExAl2Sb6system, the electricalconductivity decreased due to the canceling off of the newly addedn-type car-riers by the already existingp-type carriers resulting in still thep-type character.However, the enhanced Seebeck coefficients predicted by the increased effec-tive mass from the DFT calculations eventually improved the overall power fac-tor of the RE3+-doped title compounds.
Ashida, Hisashi,Hong, Yeongjin,Murakami, Yoshiko,Shishioh, Nobue,Sugimoto, Nakaba,Kim, Youn Uck,Maeda, Yusuke,Kinoshita, Taroh American Society for Cell Biology 2005 Molecular biology of the cell Vol.16 No.3
<P>Within the endoplasmic reticulum (ER), mannoses and glucoses, donated from dolichol-phosphate-mannose and -glucose, are transferred to N-glycan and GPI-anchor precursors, and serine/threonine residues in many proteins. Glycosyltransferases that mediate these reactions are ER-resident multitransmembrane proteins with common characteristics, forming a superfamily of >10 enzymes. Here, we report an essential component of glycosylphosphatidylinositol-mannosyltransferase I (GPI-MT-I), which transfers the first of the four mannoses in the GPI-anchor precursors. We isolated a Chinese hamster ovary (CHO) cell mutant defective in GPI-MT-I but not its catalytic component PIG-M. The mutant gene, termed phosphatidylinositolglycan-class X (PIG-X), encoded a 252-amino acid ER-resident type I transmembrane protein with a large lumenal domain. PIG-X and PIG-M formed a complex, and PIG-M expression was <10% in the absence of PIG-X, indicating that PIG-X stabilizes PIG-M. We found that Saccharomyces cerevisiae Pbn1p/YCL052Cp, which was previously reported to be involved in autoprocessing of proproteinase B, is the functional homologue of PIG-X; Pbn1p is critical for Gpi14p/YJR013Wp function, the yeast homologue of PIG-M. This is the first report of an essential subcomponent of glycosyltransferases using dolichol-phosphate-monosaccharide.</P>
나노임프린트를 이용한 마이크로-나노 혼합 스케일 PMMA 채널 네트워크 제작공정 개발
홍지수(Jisoo Hong),임영진(Yeongjin Lim),신흥주(Heungjoo Shin) 대한기계학회 2015 대한기계학회 춘추학술대회 Vol.2015 No.11
In this paper, we present the fabrication of mixed-scale PMMA (polymethyl methacrylate) channels consisting of micro- and nano-channels using simple thermal nanoimprint and thermal bonding processes (microchannels: width = ~ 100 ㎛, height = ~ 8 ㎛; nanochannels: width = ~ 700 ㎚, height = ~ 150 ㎚, length = ~ 100 ㎛). In the nanoimprint process, a monolithic mixed-scale carbon structure was used as a mold. The monolithic carbon mold was fabricated using carbon-MEMS consisting of two-step photolithography processes for patterning a polymer structure and one step pyrolysis process for converting polymer to carbon. In pyrolysis, polymer structures shrank dramatically and thus micro-sized polymer structures could be converted into sub-micro-/nano-sized carbon structures. The shape of the monolithic mixed-scale carbon mold was pressed into a PMMA sheet while the polymer sheet was heated. After demolding the carbon mold from the patterned PMMA sheet, the patterned channel networks on the PMMA sheet was sealed by bonding another thin PMMA sheet to the patterned PMMA sheet with pressure and heat subsequent to an oxygen plasma treatment. The pyrolyzed carbon mold could be easily demolded because of its curved side walls resulting from anisotropic volume reduction in pyrolysis. This special geometry and good robustness of the carbon mold ensured reproducibility in nanoimprint process. PMMA has higher Young’s modulus compared to PDMS (polydimethylsiloxane) that is widely used for the nano-channel fabrication so that the PMMA channels ensure consistent channel fabrication and nanofluidic experiments without channel collapse originated problems.
The Initial Enzyme for Glycosylphosphatidylinositol Biosynthesis Requires PIG-Y, a Seventh Component
Murakami, Yoshiko,Siripanyaphinyo, Uamporn,Hong, Yeongjin,Tashima, Yuko,Maeda, Yusuke,Kinoshita, Taroh American Society for Cell Biology 2005 Molecular biology of the cell Vol.16 No.11
<P>Biosynthesis of glycosylphosphatidylinositol (GPI) is initiated by an unusually complex GPI-N-acetylglucosaminyltransferase (GPI-GnT) consisting of at least six proteins. Here, we report that human GPI-GnT requires another component, termed PIG-Y, a 71 amino acid protein with two transmembrane domains. The Burkitt lymphoma cell line Daudi, severely defective in the surface expression of GPI-anchored proteins, was a null mutant of PIG-Y. A complex of six components was formed without PIG-Y. PIG-Y appeared to be directly associated with PIG-A, implying that PIG-Y is the key molecule that regulates GPI-GnT activity by binding directly to the catalytic subunit PIG-A. PIG-Y is probably homologous to yeast Eri1p, a component of GPI-GnT. We did not obtain evidence for a functional linkage between GPI-GnT and ras GTPases in mammalian cells as has been reported for yeast cells. A single transcript encoded PIG-Y and, to its 5′ side, another protein PreY that has homologues in a wide range of organisms and is characterized by a conserved domain termed DUF343. These two proteins are translated from one mRNA by leaky scanning of the PreY initiation site.</P>
Kim, Youn Uck,Hong, Yeongjin Korean Society for Molecular Biology 2007 Molecules and cells Vol.24 No.2
<P>The mammalian glycosylphosphatidylinositol (GPI) anchor consists of three mannoses attached to acylated GlcN-(acyl)PI to form Man(3)-GlcN-(acyl)PI. The first of the three mannose groups is attached to an intermediate to generate Man-GlcN-(acyl)PI by the first mannosyltransferase (GPI-MT-I). Mammalian and protozoan GPI-MT-I have different substrate specificities. PIG-M encodes the mammalial GPI-MT-I which has 423 amino acids and multiple transmembrane domains. In this work we cloned PIG-M homologues from humans, Plasmodium falciparum (PfPIG-M), and Saccharomyces cerevisiae (GPI14), to test whether they could complement GPI-MT-I-deficient mammalian cells, since this biosynthetic step is likely to be a good target for selective screening of inhibitors against many pathogenic organisms. PfPIG-M partially restored cell surface expression of the GPI-anchored protein CD59 in PIG-M deficient mammalian cells, and first mannose transfer activity in vitro; however, this was not the case for GPI14.</P>
Kim, Jeong Tae,Barua, Sonia,Kim, Hyeongmin,Hong, Seong-Chul,Yoo, Seung-Yup,Jeon, Hyojin,Cho, Yeongjin,Gil, Sangwon,Oh, Kyungsoo,Lee, Jaehwi The Korean Society of Applied Pharmacology 2017 Biomolecules & Therapeutics(구 응용약물학회지) Vol.25 No.4
In this study, the effect of particle size of genistein-loaded solid lipid particulate systems on drug dissolution behavior and oral bioavailability was investigated. Genistein-loaded solid lipid microparticles and nanoparticles were prepared with glyceryl palmitostearate. Except for the particle size, other properties of genistein-loaded solid lipid microparticles and nanoparticles such as particle composition and drug loading efficiency and amount were similarly controlled to mainly evaluate the effect of different particle sizes of the solid lipid particulate systems on drug dissolution behavior and oral bioavailability. The results showed that genistein-loaded solid lipid microparticles and nanoparticles exhibited a considerably increased drug dissolution rate compared to that of genistein bulk powder and suspension. The microparticles gradually released genistein as a function of time while the nanoparticles exhibited a biphasic drug release pattern, showing an initial burst drug release, followed by a sustained release. The oral bioavailability of genistein loaded in solid lipid microparticles and nanoparticles in rats was also significantly enhanced compared to that in bulk powders and the suspension. However, the bioavailability from the microparticles increased more than that from the nanoparticles mainly because the rapid drug dissolution rate and rapid absorption of genistein because of the large surface area of the genistein-solid lipid nanoparticles cleared the drug to a greater extent than the genistein-solid lipid microparticles did. Therefore, the findings of this study suggest that controlling the particle size of solid-lipid particulate systems at a micro-scale would be a promising strategy to increase the oral bioavailability of genistein.