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Yejin Kyung,jae seon Lee,hyun kyung Kim,hyun-na Koo,jeongoh Yang,bong-su Kim,byung-ho Lee,gil-hah Kim 한국응용곤충학회 2017 한국응용곤충학회 학술대회논문집 Vol.2017 No.10
Fumigation activity of Frankliniella occidentalis, western flower thrips, and phytotoxicity were evaluated with phosphine(PH3) and ethyl formate (EF) in the asparagus. Fumigation activity of PH3 and EF showed in the order of egg (64.7 mg·h/Land 132.9 mg·h/L), nymph (5.5 mg·h/L and 14.8 mg·h/L), and adult (3.8 mg·h/L and 14.7 mg·h/L) respectively at 5˚C in12 L desiccator. The adsorption of PH3 was 24% at 5˚C. However, the adsorption of EF increased sharply to 98% at 5˚C.PH3 showed 100% control for all development stage of F. occidentalis with 4g/m3 in 0.65m3 at 2˚C for 48h exposure. Thephytotoxicity was not found. The results revealed that the EF is highly absorbable in asparagus, so it is not suitable as afumigant, but PH3 is considered to be suitable as an alternative MB fumigant for control of the F. occidentalis in asparagus.
Biallelic Deletion of Pxdn in Mice Leads to Anophthalmia and Severe Eye Malformation
Kim, Hyun-Kyung,Ham, Kyung A,Lee, Seung-Woo,Choi, Hong Seok,Kim, Hong-Sug,Kim, Hong Kyung,Shin, Hae-Sol,Seo, Kyoung Yul,Cho, Yejin,Nam, Ki Taek,Kim, In-Beom,Joe, Young Ae MDPI AG 2019 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.20 No.24
<P>Peroxidasin (PXDN) is a unique peroxidase containing extracellular matrix motifs and stabilizes collagen IV networks by forming sulfilimine crosslinks. PXDN gene knockout in Caenorhabditis elegans (C. elegans) and Drosophila results in the demise at the embryonic and larval stages. PXDN mutations lead to severe eye disorders, including microphthalmia, cataract, glaucoma, and anterior segment dysgenesis in humans and mice. To investigate how PXDN loss of function affects organ development, we generated Pxdn knockout mice by deletion of exon 1 and its 5′ upstream sequences of the Pxdn gene using the CRISPR/Cas9 system. Loss of both PXDN expression and collagen IV sulfilimine cross-links was detected only in the homozygous mice, which showed completely or almost closed eyelids with small eyes, having no apparent external morphological defects in other organs. In histological analysis of eye tissues, the homozygous mice had extreme defects in eye development, including no eyeballs or drastically disorganized eye structures, whereas the heterozygous mice showed normal eye structure. Visual function tests also revealed no obvious functional abnormalities in the eyes between heterozygous mice and wild-type mice. Thus, these results suggest that PXDN activity is essential in eye development, and also indicate that a single allele of Pxdn gene is sufficient for eye-structure formation and normal visual function.</P>
Yejin Lee,Hong-Bae Yun,Jwa-Kyung Sung,Sang-Keun Ha,Yo-Sung Song,Yeon-Kyu Sonn,Deog-Bae Lee 한국토양비료학회 2014 한국토양비료학회지 Vol.47 No.6
The steady increase in livestock industry has greatly required the stable production of food and forage crops. As an alternative, rice-forage cropping system has been attempted in several southern areas. The present study was performed to understand whether an application of cattle-manure compost affects soil chemical properties and crop productivity in rice-forage cropping system, rice → summer oat → rye, in Jangheong county, south Jeolla province from 2013 to 2014. Treatments was composed of control (no compost), CM1 (compost application before rice transplanting), and CM2 (two-times compost application, before rice transplanting and after rice harvest), and inorganic fertilizers (N, P, and K) were equally dressed in all plots. Yields of rice were not significantly different between treatments, however, oat production was 1.25-fold higher in CM1 and CM2. Nutrient uptake amounts of rye were higher in CM2 than CM1 and control. Total nitrogen in soil was maintained stable level during crop cultivation. And soil organic matter contents in all treatments were increased by crop residue. Available P₂O5 and exchangeable K were increased by cattle manure application. Therefore, it suggested that the amount of nutrient by forage crop residue should be considered in rice-forage multiple cultivation.
울금 주정 추출물이 자연살해세포와 T 면역세포에 미치는 영향
하예진(Yejin Ha),김옥경(Ok-Kyung Kim),남다은(Da-Eun Nam),김용재(Yongjae Kim),김은(Eun Kim),전우진(Woojin Jun),이정민(Jeongmin Lee) 한국식품영양과학회 2015 한국식품영양과학회지 Vol.44 No.3
본 연구에서는 국내에서 재배되는 울금의 면역조절 효과에 대해 평가하고자 20% 주정 추출물을 이용하여 자연살해세포와 T 세포에 미치는 영향을 관찰하였다. 마우스의 비장세포에서 분리한 자연살해세포를 종양세포 YAC-1 세포와 함께 배양시켜 울금 20% 주정 추출물의 처리에 따른 변화를 관찰하였다. 그 결과 울금 20% 주정 추출물의 처리는 자연살해세포의 CD69 발현과 IFN-γ의 발현을 증가시켰고 결과적으로 활성이 증가되어 YAC-1 세포의 제거를 증가시켰음을 확인하였다. 또한 마우스의 비장세포에서 울금 20% 주정 추출물의 처리에 따른 T 세포의 변화를 관찰한 결과에서는 CD4+ T 세포보다는 CD8+ T 세포를 증가시켰음을 확인하여 감염된 세포나 종양세포 제거를 효과적으로 할 수 있을 것이라고 예상할 수 있었다. 결론적으로 울금 20% 주정 추출물은 내재면역과 적응면역에 영향을 미쳐 면역조절에 긍정적인 변화를 보였음을 확인하였다. The present study investigated the immunomodulatory effects of Curcuma longa L. ethanol extracts on natural killer (NK) cells and T cells. We treated Curcuma longa L. ethanol extracts at concentrations of 20, 50, 100, and 150 μg/mL to murine NK cells co-incubated with YAC-1 cells. Curcuma longa L. ethanol extracts resulted in increased NK cell activity compared to the control group at all concentrations. In the groups treated with Curcuma longa L. ethanol extracts, CD69 and IFN-γ expression levels significantly increased compared to the control group at 100 and 150 μg/mL. In addition, Curcuma longa L. ethanol extracts induced significant elevation of CD8+ T cell numbers in a dose-dependent manner. However, Curcuma longa L. ethanol extracts also led to reduction of CD4+ T cell and MHCⅡ numbers. The findings of this study suggest that Curcuma longa L. ethanol extracts could enhance the immune response through activation of NK and cytotoxic T cells due to a proliferative shift of antigen presentation from MHCⅡ to MHCⅠ, presumably.
Lee, Yejin,Yun, Hong-Bae,Sung, Jwa-Kyung,Ha, Sang-Keun,Song, Yo-Sung,Sonn, Yeon-Kyu,Lee, Deog-Bae Korean Society of Soil Science and Fertilizer 2014 한국토양비료학회지 Vol.47 No.6
The steady increase in livestock industry has greatly required the stable production of food and forage crops. As an alternative, rice-forage cropping system has been attempted in several southern areas. The present study was performed to understand whether an application of cattle-manure compost affects soil chemical properties and crop productivity in rice-forage cropping system, rice ${\rightarrow}$ summer oat ${\rightarrow}$ rye, in Jangheong county, south Jeolla province from 2013 to 2014. Treatments was composed of control (no compost), CM1 (compost application before rice transplanting), and CM2 (two-times compost application, before rice transplanting and after rice harvest), and inorganic fertilizers (N, P, and K) were equally dressed in all plots. Yields of rice were not significantly different between treatments, however, oat production was 1.25-fold higher in CM1 and CM2. Nutrient uptake amounts of rye were higher in CM2 than CM1 and control. Total nitrogen in soil was maintained stable level during crop cultivation. And soil organic matter contents in all treatments were increased by crop residue. Available P_2O_5$ and exchangeable K were increased by cattle manure application. Therefore, it suggested that the amount of nutrient by forage crop residue should be considered in rice-forage multiple cultivation.
대중교통 내 교통약자와 비교통약자 간의 의사소통 서비스 디자인 제안교통 : 약자 중에서도 임산부를 대상으로
신예진(Shin,YeJin),송진경(Song,Jin Kyung),정가람(Jeong, Ga Ram),김다운(Kim, Daun),박수이(Park, Sue) 한국디자인리서치학회 2019 한국디자인리서치 Vol.4 No.1
교통약자 중에서도 임산부는 대중교통 수단의 이용이 어려운 승객 중 하나이다.본 연구의 목적은 대중교통 내 교통약자의 배려를 위한 교통약자와 비교통약자의 간의 원활한 의사소통을 위한 의사소통 서비스 디자인을 제안하고자 한다.이를 위하여 사전 연구에서는 임산부를 대상으로 설문과FGI를 진행하였다.연구1에서는 앞서 진행한 사전연구의 결과를 바탕으로 모바일 기반의 교통약자와 비교통약자 간의 의사소통 서비스를 실제로 구현하였다. 마지막으로 연구 2에서는 구현한 결과물을 실제 지하철의 환경에서 사용자 평가 통해 효과적임을 검증하였다.본 연구는 교통약자와 비교통약자 두 그룹 간의 의사소통을 통해 임산부 승객에게 도움이 될 만한 서비스를 실제로 구현했다는 점에서 의의를 가진다. Among Mobility Handicapped, pregnant women are passengers who have difficulty using public transportation. The purpose of this study is to propose the design of a communicationservice for smooth communication between Mobility Handicapped and the regular passenger in public transport. For this purpose, we conducted a questionnaire and FGI for pregnant women in the pre-study. In Study 1, based on the results of the previous study, weproposed mobile-based communication services between the Mobility Handicapped and the regular passenger. Finally, in Study 3, user evaluation of services was carried out. As a result, we implemented and verified effective communication services between pregnant passengers and ordinary passengers, who are transportation users in public transportation by utilizing mobile apps.This study has significance in that it implemented services that would be useful to pregnant passengers through communication between the twogroups of the mobility Handicapped and the regular passenger.