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Structure and Photoreaction of Photoactive Yellow Protein
Imamoto, Yasushi,Harigai, Miki,Shimizu, Nobutaka,Kamikubo, Hironari,Yamazaki, Yoichi,Kataoka, Mikio Korean Society of Photoscience 2002 Journal of Photosciences Vol.9 No.2
The chromophore/protein interactions in the photocycle intermediates of photoactive yel- low protein (PYP) were probed by site-directed mutagenesis. The absorption spectra of L- intermediates produced from E46Q, T50V, and R52Q mutants were calculated using the absorption spectra of dark states and difference absorption spectra between L-intermediates and dark states, and compared with that of PYP$\_$L/. The absorption spectrum of R52Q$\_$L/ agreed with that of PYP$\_$L/, but those of E46Q$\_$L/ and T50V$\_$L/ were red-shifted. The effect of these mutations on the absorption spectrum for L-intermediate was comparable to that for the dark state, suggesting that the interaction around the phe-nolic oxygen of the chromophore is conserved in PYP$\_$L/ unlike the crystal structure. On the other hand, we have reported that the absorption spectra of Y 42F$\_$M/, T50V $\_$M/, and R52Q$\_$M/ agreed with that of PYP$\_$M/, but that of E46Q$\_$M/ was red-shifted, suggesting that the hydrogen bond of the chromophore with Glu46 is conserved but that with Tyr42 is broken in PYP$\_$M/. These results suggest that the chromophore inter-acts with Glu46 throughout the photocycle, but never directly interacts with Arg52. This model con- flicts with some of the structural model of PYP intermediates proposed based on the high-resolution X -ray crystallography.
Short-Term Student Exchanges for Aspiring International Activities of Engineering Students
Sato, Takashi,Sakamoto, Shuichi,Shimizu, Tadaaki,Suzuki, Takamasa,Ikeda, Hideki,Hopf, Anja,Goto, Yasushi,Oka, Tetsuo,Sasaki, Tomohiro,Narumi, Takatsune Korean Society for Engineering Education 2014 공학교육연구 Vol.17 No.4
The Faculty of Engineering, Niigata University, Japan, obtained the scholarship in "Short-Stay and Short-Visit scholarship programs(SS-SV programs)" for both German and Japanese students in 2011 and 2012. Our short-term student exchange programs proved that a short-term stay in a foreign country worked as an initiator for the students' interest and it strengthen their will to study further more in foreign countries.
Short-Term Student Exchanges for Aspiring International Activities of Engineering Students
Takashi Sato,Shuichi Sakamoto,Tadaaki Shimizu,Takamasa Suzuki,Hideki Ikeda,Anja Hopf,Yasushi Goto,Tetsuo Oka,Tomohiro Sasaki,Takatsune Narumi 한국공학교육학회 2014 공학교육연구 Vol.17 No.4
The Faculty of Engineering, Niigata University, Japan, obtained the scholarship in “Short-Stay and Short-Visit scholarshipprograms(SS-SV programs)” for both German and Japanese students in 2011 and 2012. Our short-term student exchange programsproved that a short-term stay in a foreign country worked as an initiator for the students' interest and it strengthen their will tostudy further more in foreign countries.
A Proposal of Wheel/Rail Contact Model for Friction Control
Kosuke Matsumoto,Yoshihiro Suda,Hisanao Komine,Takuji Nakai,Masao Tomeoka,Kunihito Shimizu,Masuhisa Tanimoto,Yasushi Kishimoto,Takashi Fujii 대한기계학회 2005 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.19 No.1S
Controlling the friction between wheel and rail is direct and very effective measures to Improve the curving performances of railway trucks, because the curving performances depend much on friction characteristics Authors have proposed a method, "fllction control", which utilizes friction modifier (KELTRACKTM HPF) with onboard spraying system With the method, not only friction coefficient, but also fuction characteristics can be controlled as expected In this study, MBD simulation is very valuable tool to foresee the effect of the contiol In advance of experiment With real car And the creep characteristics of wheel/rail contact With the friction modifier takes very Important role In the simulation In this paper, authors propose a theoretical model of wheel/rat I contact condition considering the creep characteristics of friction modifier, Which is derived the application of principle tribological theories<br/>
Fujio Ito,Zenya Ito,Motohide Shibayama,Shu Nakamura,Minoru Yamada,Hideki Yoshimatu,Mikinobu Takeuchi,Kenzo Shimizu,Yasushi Miura 대한척추신경외과학회 2019 Neurospine Vol.16 No.1
Objective: Spinal stenosis is increasingly common due to population aging. In elderly patients with lumbar central canal stenosis (LCCS), minimizing muscle damage and bone resection is particularly important. We performed a step-by-step operation with a newly designed spinal endoscope to obtain adequate decompression in patients with spinal stenosis. Methods: From April 2015 to August 2016, 78 patients (48 males, 30 females) with LCCS (91 segments) underwent endoscopic decompression using a newly designed endoscope system. The inclusion criteria were: (1) neurogenic intermittent claudication with or without radiculopathy, (2) LCCS, and (3) having exhausted conservative treatment (>3 months). The exclusion criteria were: (1) >10° of instability, (2) spondylolisthesis grade II or greater according to the Meyerding criteria, (3) foraminal stenosis, (4) vascular intermittent claudication, (5) infection, and (6) stenosis combined with malignancy. We performed a step-by-step procedure using a newly designed endoscope system for unilateral-approach bilateral decompression. We used the same incision for 2–3 segments, only moving the skin. Results: The mean follow-up was 2.3±1.3 years. Excellent or good results were found according to the MacNab criteria in 85.9% of cases (67 of 78). The visual analogue scale, Japanese Orthopedic Association score, and Oswestry Disability Index showed significant decreases at 1 month, persisting until the 2-year follow-up. Dural tear occurred in 4 cases (5.1%), and patch repair was performed under endoscopy. No patients experienced aggravated instability requiring surgery. Conclusion: We obtained good results with endoscopic decompression surgery using a newly designed instrument that minimized muscle and bone damage in elderly patients with spinal stenosis.