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        Finite Frequency Positive Real Control for Singularly Perturbed Systems

        Yanlong Huang,Chenxiao Cai,Yun Zou 제어·로봇·시스템학회 2011 International Journal of Control, Automation, and Vol.9 No.2

        In this paper, strictly positive real control for singularly perturbed systems in (semi)finite frequency ranges is studied. For the general linear systems, necessary and sufficient conditions for the existence of a stabilizing state feedback controller are given based on the generalized KYP lemma, and use the results to study singularly perturbed systems, a composite state feedback controller is constructed, which preserves the stability and positive real property.

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        Influence of δ' Phase with GP‑I Zones Fillings on Slip Behavior and Cold Rolling Texture in AA2099

        Xusheng Yang,Weijiu Huang,Xianghui Zhu,Fei Guo,Yanlong Ma,Linjiang Chai,Ran Zhang 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.9

        The cold rolling texture of AA2099 sheets was strongly influenced by the prior heat treatment. Two typical heat treatments,including solid solution (T4) and underaging (T6) were utilized to control slip behavior by different precipitates during coldrolling. A much weaker texture with an orientation scatter towards higher Φ angles was found in the as-rolled T6 specimen(T6R). Slip behavior was analyzed using geometry necessary boundaries distribution. Results show that the T6R specimenexperienced a strong co-planar slip compared to the as-rolled T4 specimen. Prosperity of co-planar slips and limitation ofco-direction slips were main reasons for the weakening of Copper texture component in the T6R specimen. The distinguishslip behavior was caused by existence of δ' phase with GP-I zones fillings in the T6 specimen. This phase could be easilysheared by dislocations and then promoted co-planar slips in further deformation.

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        Surface-Displayed Porcine IFN-λ3 in Lactobacillus plantarum Inhibits Porcine Enteric Coronavirus Infection of Porcine Intestinal Epithelial Cells

        Yong-Shi Liu,Qiong Liu,Yanlong Jiang,Wentao Yang,Hai-Bin Huang,Chun-Wei Shi,Gui-Lian Yang,Chun-Feng Wang 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.4

        Interferon (IFN)-λ plays an essential role in mucosal cells which exhibit strong antiviral activity. Lactobacillus plantarum (L. plantarum) has substantial application potential in the food and medical industries because of its probiotic properties. Alphacoronaviruses, especially porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV), cause high morbidity and mortality in piglets resulting in economic loss. Co-infection by these two viruses is becoming increasingly frequent. Therefore, it is particularly important to develop a new drug to prevent diarrhea infected with mixed viruses in piglets. In this study, we first constructed an anchored expression vector with CWA (C-terminal cell wall anchor) on L. plantarum. Second, we constructed two recombinant L. plantarum strains that anchored IFN-λ3 via pgsA (N-terminal transmembrane anchor) and CWA. Third, we demonstrated that both recombinant strains possess strong antiviral effects against coronavirus infection in the intestinal porcine epithelial cell line J2 (IPEC-J2). However, recombinant L. plantarum with the CWA anchor exhibited a more powerful antiviral effect than recombinant L. plantarum with pgsA. Consistent with this finding, Lb.plantarum-pSIP-409-IFN- λ3-CWA enhanced the expression levels of IFN-stimulated genes (ISGs) (ISG15, OASL, and Mx1) in IPEC-J2 cells more than did recombinant Lb.plantarum-pSIP-409-pgsA'-IFN-λ3. Our study verifies that recombinant L. plantarum inhibits PEDV and TGEV infection in IPEC-J2 cells, which may offer great potential for use as a novel oral antiviral agent in therapeutic applications for combating porcine epidemic diarrhea and transmissible gastroenteritis. This study is the first to show that recombinant L. plantarum suppresses PEDV and TGEV infection of IPEC-J2 cells.

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