Mitochondrial citrate accumulation drives alveolar epithelial cell necroptosis in lipopolysaccharide-induced acute lung injury

Yang Hui-Hui,Jiang Hui-Ling,Tao Jia-Hao,Zhang Chen-Yu,Xiong Jian-Bing,Yang Jin-Tong,Liu Yu-Biao,Zhong Wen-Jing,Guan Xin-Xin,Duan Jia-Xi,Zhang Yan-Feng,Liu Shao-Kun,Jiang Jian-Xin,Zhou Yong,Guan Cha-Xi 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Necroptosis is the major cause of death in alveolar epithelial cells (AECs) during acute lung injury (ALI). Here, we report a previously unrecognized mechanism for necroptosis. We found an accumulation of mitochondrial citrate (citratemt) in lipopolysaccharide (LPS)-treated AECs because of the downregulation of Idh3α and citrate carrier (CIC, also known as Slc25a1). shRNA- or inhibitor–mediated inhibition of Idh3α and Slc25a1 induced citratemt accumulation and necroptosis in vitro. Mice with AEC-specific Idh3α and Slc25a1 deficiency exhibited exacerbated lung injury and AEC necroptosis. Interestingly, the overexpression of Idh3α and Slc25a1 decreased citratemt levels and rescued AECs from necroptosis. Mechanistically, citratemt accumulation induced mitochondrial fission and excessive mitophagy in AECs. Furthermore, citratemt directly interacted with FUN14 domain-containing protein 1 (FUNDC1) and promoted the interaction of FUNDC1 with dynamin-related protein 1 (DRP1), leading to excessive mitophagy-mediated necroptosis and thereby initiating and promoting ALI. Importantly, necroptosis induced by citratemt accumulation was inhibited in FUNDC1-knockout AECs. We show that citratemt accumulation is a novel target for protection against ALI involving necroptosis.

Experimental study on CO2 bubble dynamics under different solution viscosity and absorbent concentration

Yang Jia-xi,Gao Dan,Qi You-wei,Zhang Heng 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.8

Carbon dioxide (CO2) emitted by fossil energy combustion is related to the greenhouse effect. To furtherstudy the motion dynamics of CO2 bubbles in various solutions so as to better absorb them, a CO2 bubble experimentalplatform was built. The growth and motion of a single CO2 bubble were experimented in five concentrations ofNaOH, NaHCO3 solutions and five viscosity deionized waters, photographed with a high-speed camera and importedinto PyCharm for analysis. Based on this, four kinds of CO2 bubbles were experimentally studied. The results show thatthe viscosity leads to the increase of rising time; the maximum rise time is 0.518 s when the viscosity is 100 mPa·s, theaspect ratio  of CO2 bubble in solution, there will be an “L” distribution, and the minimum rise time is close to risingafter the bubble is separated from the injector. NaHCO3 inhibits the reaction between NaOH solution and CO2, resultingin the cross-section ratio  change decreasing. The concentration of NaOH solution most conducive to CO2absorption is 0.039 g/ml and 0.058 g/ml.

Fenofibrate Increases Radiosensitivity in Head and Neck Squamous Cell Carcinoma via Inducing G2/M Arrest and Apoptosis

Liu, Jia,Ge, Yang-Yang,Zhu, Hong-Cheng,Yang, Xi,Cai, Jing,Zhang, Chi,Lu, Jing,Zhan, Liang-Liang,Qin, Qin,Yang, Yan,Yang, Yue-Hua,Zhang, Hao,Chen, Xiao-Chen,Liu, Zhe-Ming,Ma, Jian-Xin,Cheng, Hong-Yan,S Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.16

Radiation therapy is an important treatment for head and neck squamous cell carcinoma (HNSCC). However, how to promote radiation sensitivity in HNSCC remains a challenge. This study aimed to investigate the radiosensitizing effects of fenofibrate on HNSCC and explore the underlying mechanisms. HNSCC cell lines CNE-2 and KB were subjected to ionizing radiation (IR), in the presence or absence of fenofibrate treatment. Cell growth and survival, apoptosis and cell cycle were evaluated. In addition, CNE-2 cells were xenografted into nude mice and subjected to IR and/or fenofibrate treatment. The expression of cyclinB and CDK1 was detected by Western blotting. Our results showed that fenofibrate efficiently radiosensitized HNSCC cells and xenografts in mice, and induced apoptosis and G2/M arrest via reducing the activity of the CDK1/cyclinB1 kinase complex. These data suggest that fenofibrate could be a promising radiosensitizer for HNSCC radiotherapy.

왕취엔안(王全安) 영화의 특징에 대한 연구

양가희 ( Jia Xi Yang ),박상현 ( Sang Hyun Park ) 커뮤니케이션디자인학회 2016 커뮤니케이션 디자인학연구 Vol.54 No.-

왕취엔안은 중국 제6세대 감독 중의 하나로서 그의 개인적 시선에 의한 독특한 독립영화를 만들어 왔다. 다른 제6세대 감독들과 비교해 그의 영화의 작품성에 비하여 상대적으로 그는 중국내에서는 크게 주목을 받지 못했다. 그러나 그의 영화는 작품성을 인정받아 여러 국제영화제에서 수상하였다. 본 연구에서는 왕취엔안의 영화를 시간상으로 관찰하여 선명한 개인적 스타일이 점차적으로 확립되는 과정을 발견할 수 있다. 본고는 시간 순으로 왕취엔안의 6편의 영화를 내용적 형식적 특징을 연구함으로써 그 영화의 예술적특징과 맥락 그리고 그의 영화의 개인적 스타일의 형성 과정을 알아보았다. 그의 영화에서는 다큐멘터리적인 시선으로 롱테이크를 즐겨 사용하는 것, 그리고 주제적으로 소외된 중국 소시민의 생활을 주로 관심 있게 다루고 있다는 특징을 살펴볼 수 있었다. Wang Quanan is one of China 6th director, is also famous for independent film. However, Wang Quanan is not so famous in China while he received lots of reward in overseas. Wang Quanan shows strong personal style through his films. This paper mainly analysis 6 films of Wang Quanan in detail. Such as art characteristics, unique charm of his film, forming process of his personal style. So we can find the characteristics of his film, that he uses formally the long take for documentary style and is thematically interested about in disadvantaged Chinese citizens.

Experimental Investigations of Natural Convection in Wire-Woven Bulk Kagome

Yang, Xiao Hu,Bai, Jia Xi,Kang, Ki-Ju,Lu, Tian Jian,Kim, Tongbeum Springer-Verlag 2014 Transport in porous media Vol.105 No.1

HER2-enriched Tumors Have the Highest Risk of Local Recurrence in Chinese Patients Treated with Breast Conservation Therapy

Jia, Wei-Juan,Jia, Hai-Xia,Feng, Hui-Yi,Yang, Ya-Ping,Chen, Kai,Su, Feng-Xi Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.1

Purpose: The purpose of this study was to investigate the recurrence pattern and characteristics of patients based on the 2013 St. Gallen surrogate molecular subtypes after breast-conserving surgery (BCS) in Chinese women. Methods: This retrospective analysis included 709 consecutive breast cancer patients undergoing BCS from 1999-2010 at our institution. Five different surrogate subtypes were created using combined expression of the estrogen receptor, progesterone receptor, and human epidermal growth factor receptor-2. Locoregional relapse-free survival (LRRFS), distant metastasis-free survival (DMFS), and disease-free survival (DFS) rates were calculated. Results: The 5-year LRRFS, DMFS, and DFS rates were 90.5%, 88.2%, and 81.5%, respectively. Multivariate analysis revealed that young age, node-positive disease, and HER2 enrichment were independent prognostic factors in LRRFS patients. There was also an independent prognostic role of lymph node-positive disease in DMFS and DFS patients. Patients with luminal A tumors had the most favorable prognosis, with LRRFS, DMFS, and DFS rates of 93.2%, 91.5%, and 87.4% at 5 years, respectively. Conversely, HER-2-enriched tumors exhibited the highest rate of locoregional recurrence (20.6%). Conclusion: Surrogate subtypes present with significant differences in RFS, DMFS, and LRRFS. Luminal A tumors have the best prognosis, whereas HER2-enriched tumors have the poorest.

NPC-IGCT Phase Module Clamp Circuit LRC Parameters Design considering FRD snappy Recovery

Yang Ju Zou,Jia Xi Hu,Zhen Yu Ma,Jian Ping Liu,Run Qing Guo,Zhi Xue Zhang 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5

In this paper, a method for neutral point clamping-integrated gate commutated thyristor (NPCIGCT) phase module clamp circuit parameters design considering FRD snappy recovery is present. Based on the snappy recovery theory, and analysis of snappy recovery factors, the paper has shown decreasing current commutating slope can attenuated snappy recovery affect effectively. Then, the paper has shown that it is reasonable for decreasing di/dt by increasing inductance of NPC-IGCT phase module clamp circuit, based on the circuit working principle. Then, the new clamp circuit parameters design method which combine multi-objective optimization solution mathematical module of the circuit, fast recovery diode (FRD) snappy recovery, devices overvoltage and loss is shown in paper. A design example and its test results have demonstrated both IGCT and FRD characteristics have been guaranteed and ensuring safety and reliability of the NPC - IGCT phase module.

A Novel Phase Locked Loop for Grid-Connected Converters under Non-Ideal Grid Conditions

Yang, Long-Yue,Wang, Chong-Lin,Liu, Jian-Hua,Jia, Chen-Xi The Korean Institute of Power Electronics 2015 JOURNAL OF POWER ELECTRONICS Vol.15 No.1

Grid synchronization is one of the key techniques for the grid-connected power converters used in distributed power generation systems. In order to achieve fast and accurate grid synchronization, a new phase locked loop (PLL) is proposed on the basis of the complex filter matrixes (CFM) orthogonal signal generator (OSG) crossing-decoupling method. By combining first-order complex filters with relation matrixes of positive and negative sequence voltage components, the OSG is designed to extract specific frequency orthogonal signals. Then, the OSG mathematical model is built in the frequency-domain and time-domain to analyze the spectral characteristics. Moreover, a crossing-decoupling method is suggested to decouple the fundamental voltage. From the eigenvalue analysis point of view, the stability and dynamic performance of the new PLL method is evaluated. Meanwhile, the digital implementation method is also provided. Finally, the effectiveness of the proposed method is verified by experiments under unbalanced and distorted grid voltage conditions.

Dose-Dependent Associations between Wine Drinking and Breast Cancer Risk - Meta-Analysis Findings

Chen, Jia-Yan,Zhu, Hong-Cheng,Guo, Qing,Shu, Zheng,Bao, Xu-Hui,Sun, Feng,Qin, Qin,Yang, Xi,Zhang, Chi,Cheng, Hong-Yan,Sun, Xin-Chen Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.3

Purpose: To investigate any potential association between wine and breast cancer risk. Materials and Methods: We quantitatively assessed associations by conducting a meta-analysis based on evidence from observational studies. In May 2014, we performed electronic searches in PubMed, EmBase and the Cochrane Library to identify studies examining the effect of wine drinking on breast cancer incidence. The relative risk (RR) or odds ratio (OR) were used to measure any such association. Results: The analysis was further stratified by confounding factors that could influence the results. A total of twenty-six studies (eight case-control and eighteen cohort studies) involving 21,149 cases were included in our meta-analysis. Our study demonstrated that wine drinking was associated with breast cancer risk. A 36% increase in breast cancer risk was observed across overall studies based on the highest versus lowest model, with a combined RR of 1.0059 (95%CI 0.97-1.05) in dose-response analysis. However, 5 g/d ethanol from wine seemed to have protective value from our non-linear model. Conclusions: Our findings indicate that wine drinking is associated with breast cancer risk in a dose-dependent manner. High consumption of wine contributes to breast cancer risk with protection exerted by low doses. Further investigations are needed for clarification.

Liver Cancer Mortality Characteristics and Trends in China from 1991 to 2012

Fang, Jia-Ying,Wu, Ku-Sheng,Zeng, Yang,Tang, Wen-Rui,Du, Pei-Ling,Xu, Zhen-Xi,Xu, Xiao-Ling,Luo, Jia-Yi,Lin, Kun Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.5

Purpose: To investigate the distribution of liver cancer mortality as well as its developing trend from 1991 to 2012, forecast the future five-year trend, and provide a basis for the comprehensive prevention and management. Materials and Methods: Mortality data for liver cancer in China from 1991 to 2012 were used to describe characteristics and distribution of liver cancer mortality. Trend surface analysis was used to study the geographical distribution of liver cancer mortality. Curve estimation, time series modeling, gray modeling (GM) and joinpoint regression were used to predict and forecast future trends. Results: The mortality rate of liver cancer has constantly increased in China since 1991. Rates in rural areas are higher than in urban areas, and in males are higher than in females. In addition, our data predicted that the trend will continue to increase in the next 5 years. The age-specific mortality of liver cancer increases with age and peaks in the group of 80-84 years old. Geographical analysis showed the liver mortality rate was higher in the southeast provinces, such as Jiangsu, Zhejiang and Guangdong, and southwest regions like Guangxi Province. Conclusions: The standardized mortality rate of liver cancer in China has consistently increased from 1991 to 2012, and the upward trend is predicted to continue in the future. Much better prevention and management of liver cancer is needed in high mortality areas (the southwestern and southeastern parts of China) and high mortality age groups (80- to 84-year-olds), especially in rural areas.