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Stabilization of iron-arsenic deposits by encapsulation with montmorillonite inorganic gels
Jingmin Yan,Yongliang Wang,Yanhua Wang,Xiang Liu,Shufeng Ye 대한환경공학회 2022 Environmental Engineering Research Vol.27 No.2
Encapsulation is recognized as an effective technique for enhancing the stability of hazardous waste by coating it with an inert material. In this work, an eco-friendly montmorillonite (Mt) inorganic gel with the characteristics of high viscosity, adsorption and easy preparation was developed and coated on the surface of iron-arsenic deposits (IAD) to restrain the release of arsenic (As). The encapsulation system investigated involves Mt/IAD mass ratio and aging temperature of the coated iron-arsenic deposits (C-IAD). The C-IAD was characterized by XRF, XRD, SEM-EDS, TEM, FTIR and BET. The results revealed that the IAD was completely encapsulated by the montmorillonite inorganic gel. From the experiment of stability, when IAD was coated with montmorillonite inorganic gel (Mt/IAD mass ratio 0.75) and aged at 25℃, As release decreased from 3.15 mg/L to 0.64 mg/L at pH 5 after 24 h, and then dropped to 0.11 mg/L after 7 d. Furthermore, the results indicated that the IAD encapsulated with montmorillonite inorganic gel was effective in suppressing the release of As under both weakly acidic and alkaline conditions.
Tian, Shuo,Huang, Yan,Lai, Dong,Wang, Hanfeng,Du, Songliang,Shen, Donglai,Chen, Weihao,Xuan, Yundong,Lu, Yongliang,Feng, Huayi,Zhang, Xiangyi,Zhao, Wenlei,Wang, Chenfeng,Wang, Tao,Wu, Shengpan,Huang, Techno-Press 2022 Advances in nano research Vol.12 No.6
The prolyl 3-hydroxylase family member 4 (P3H4), is associated with post-translational modification of fibrillar collagens and aberrantly activated in cancer leading to tumor progression. However, its role in clear cell renal cell carcinoma (ccRCC) is still unknown. Here we reported that P3H4 was highly expressed in renal cancer tissues and significantly positive correlated with poor prognosis. Knockdown of P3H4 inhibited the proliferation, migration and metastasis of renal cancer cells in vitro and in vivo, and also, overexpression of it enhanced the oncogenic process. Mechanistically, P3H4 depletion decreased the levels of GDF15-MMP9 axis and repressed its downstream signaling. Further functional studies revealed that inhibition of GDF15 suppressed renal cancer cell growth and GDF15 recombinant human protein (rhGDF15) supplementation effectively rescued the inhibitory effect induced by P3H4 knockdown. Moreover, decreased levels of MMP9 caused by inhibition of P3H4-GDF15 signaling constrained the expression of PD-L1 and suppression of P3H4 accordingly promoted anti-tumor immunity via stimulating the infiltration of CD4+ and CD8+ T cells in syngeneic mice model. Taken together, our findings firstly demonstrated that P3H4 promotes ccRCC progression by activating GDF15-MMP9-PD-L1 axis and targeting P3H4-GDF15-MMP9 signaling pathway can be a novel strategy of controlling ccRCC malignancy.
Revitalization and Re-cultivation of Vegetation in Karst Region Through Animal
Zhiqiang Lai,Xian-feng Yi,Na Yao,Xiao-Yan Cai,Yongliang Liang,Shize Qin,Yuanrong Chen,Shangmin Qin,M. Pogaonik 한국초지조사료학회 2009 한국초지조사료학회 학술대회논문집 Vol.2009 No.08
From 2002 to 2007, two different systems (shrubs and grasses) were established to raise appropriate ruminants for the purpose of improving biological diversity and fragile ecological environment in the karst-areas through grazing. The objective was to find out a novel way to promote the application of ecological restoration and reconstruction, and the agricultural economy could develop in sustainable way in the karst areas in Guangxi and similar areas in Southwest China.
( Yunlei Han ),( Rui Wang ),( Zhirong Yang ),( Yuhua Zhan ),( Yao Ma ),( Shuzhen Ping ),( Liwen Zhang ),( Min Lin ),( Yongliang Yan ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.7
1-Aminocyclopropane-1-carboxylate (ACC) deaminase, which is encoded by some bacteria, can reduce the amount of ethylene, a root elongation inhibitor, and stimulate the growth of plants under various environmental stresses. The presence of ACC deaminase activity and the regulation of ACC in several rhizospheric bacteria have been reported. The nitrogen-fixing Pseudomonas stutzeri A1501 is capable of endophytic association with rice plants and promotes the growth of rice. However, the functional identification of ACC deaminase has not been performed. In this study, the proposed effect of ACC deaminase in P. stutzeri A1501 was investigated. Genome mining showed that P. stutzeri A1501 carries a single gene encodingACC deaminase, designated acdS. The acdS mutant was devoid of ACC deaminase activity and was less resistant to NaCl and NiCl2 compared with the wild-type. Furthermore, inactivation of acdS greatly impaired its nitrogenase activity under salt stress conditions. It was also observed that mutation of the acdS gene led to loss of the ability to promote the growth of rice under salt or heavy metal stress. Taken together, this study illustrates the essential role of ACC deaminase, not only in enhancing the salt or heavy metal tolerance of bacteria but also in improving the growth of plants, and provides a theoretical basis for studying the interaction between plant growth-promoting rhizobacteria and plants.
( Ahmed E. Gomaa ),( Zhiping Deng ),( Zhimin Yang ),( Liguo Shang ),( Yuhua Zhan ),( Wei Lu ),( Min Lin ),( Yongliang Yan ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.2
The complexity of the bacterial recombination system is a barrier for the construction of bacterial mutants for the further functional investigation of specific genes. Several protocols have been developed to inactivate genes from the genus Pseudomonas. Those protocols are complicated and time-consuming and mostly do not enable easy construction of multiple knock-ins/outs. The current study describes a single and double crossover-recombination system using an optimized vector-free allele-exchange protocol for gene disruption and gene replacement in a single species of the family Pseudomonadaceae. The protocol is based on selfligation (circularization) for the DNA cassette which has been obtained by overlapping polymerase chain reaction (Fusion-PCR), and carries an antibiotic resistance cassette flanked by homologous internal regions of the target locus. To establish the reproducibility of the approach, three different chromosomal genes (ncRNA31, rpoN, rpoS) were knocked-out from the root-associative bacterium Pseudomonas stutzeri A1501. The results showed that the P. stutzeri A1501 mutants, which are free of any plasmid backbone, could be obtained via a single or double crossover recombination. In order to optimize this protocol, three key factors that were found to have great effect on the efficiency of the homologous recombination were further investigated. Moreover, the modified protocol does not require further cloning steps, and it enables the construction of multiple gene knock-in/out mutants sequentially. This work provides a simple and rapid mutagenesis strategy for genome editing in P. stutzeri, which may also be applicable for other gram-negative bacteria.