Isolation of feline panleukopenia virus from Yanji of China and molecular epidemiology from 2021 to 2022

Haowen Xue,Chunyi Hu,Haoyuan Ma,Yanhao Song,Kunru Zhu,Jingfeng Fu,Biying Mu,Xu Gao 대한수의학회 2023 Journal of Veterinary Science Vol.24 No.2

Background: Feline panleukopenia virus (FPV) is a widespread and highly infectious pathogen in cats with a high mortality rate. Although Yanji has a developed cat breeding industry, the variation of FPV locally is still unclear. Objectives: This study aimed to isolate and investigate the epidemiology of FPV in Yanji between 2021 and 2022. Methods: A strain of FPV was isolated from F81 cells. Cats suspected of FPV infection (n = 80) between 2021 and 2022 from Yanji were enrolled in this study. The capsid protein 2 (VP2) of FPV was amplified. It was cloned into the pMD-19T vector and transformed into a competent Escherichia coli strain. The positive colonies were analyzed via VP2 Sanger sequencing. A phylogenetic analysis based on a VP2 coding sequence was performed to identify the genetic relationships between the strains. Results: An FPV strain named YBYJ-1 was successfully isolated. The virus diameter was approximately 20–24 nm, 50% tissue culture infectious dose = 1 × 10−4.94/mL, which caused cytopathic effect in F81 cells. The epidemiological survey from 2021 to 2022 showed that 27 of the 80 samples were FPV-positive. Additionally, three strains positive for CPV-2c were unexpectedly found. Phylogenetic analysis showed that most of the 27 FPV strains belonged to the same group, and no mutations were found in the critical amino acids. Conclusions: A local FPV strain named YBYJ-1 was successfully isolated. There was no critical mutation in FPV in Yanji, but some cases with CPV-2c infected cats were identified.

The Effects of Fucosylated Chondroitin Sulfate Isolated from Isostichopus badionotus on Antimetastatic Activity via Downregulation of Hif-1α and Hpa

Min He,Jingfeng Wang,Shiwei Hu,Yuming Wang,Changhu Xue,Hui Li 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.5

Fucosylated chondroitin sulfate (CHS) wasprepared from the sea cucumber Isostichopus badionotus(Ib-CHS), and the antimetastatic effect was studied usingcell adhesion, migration, invasion, chorioallntoic membrane(CAM), and reverse transcriptase-polymerase chain reaction(RT-PCR) assays. Ib-CHS remarkably suppressed proliferationof 95D cells, and inhibited adhesion, migration, and invasionof 95D cells in a dose-dependent manner. CAM assayresults suggested that Ib-CHS attenuates neovascularizationin the chick embryo. RT-PCR analyses revealed that Ib-CHS dramatically decreased expression of matrix metalloproteinase-2/9 (MMP-2/9), hypoxia-inducible factor (Hif-1α), heparanase (Hpa), and vascular endothelial growthfactor (VEGF), and increased expression levels of the tissueinhibitors of metalloproteinase-1/2 (TIMP-1/2), importantinhibitors of MMP-2/9. Ib-CHS markedly inhibited tumorgrowth, improved the metastatic foci occurrence rate, anddown-regulated expressions of Hif-1α, Hpa, and VEGF invivo. Ib-CHS exerted antimetastatic activities in vitro andin vivo.

Research on the Defense Method of Vtable Hijacking

Wang Zixiang,Shan Chun,Xue Jingfeng,Sun Shiyouhu Changzhen 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.11

Memory corruption vulnerability is an oldest type of vulnerabilities in software vulnerabilities. Attackers typically use a technique called virtual function table hijacking to exploit memory corruption vulnerability. In this paper, we propose a defense method which extracting virtual function tables and virtual function call related location information from the binary program. Then instrumenting identifier on vtables or backuping the vtables’ pointers to detect vtables’ integrity. Finally, the defense method is verified by Firefox, Chrome, IE browsers. Experiments show that the method can fully and effectively defend the real-world virtual function table hijacking attack with the small performance overhead and good compatibility.

Long-chain bases from Cucumaria frondosa inhibit adipogenesis and regulate lipid metabolism in 3T3-L1 adipocytes

Yingying Tian,Shiwei Hu,Hui Xu,Jingfeng Wang,Changhu Xue,Yuming Wang 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.6

This study aims to investigate anti-adipogenic effects of long-chain bases from Cucumaria frondosa (Cf-LCBs) in vitro. Results showed that Cf-LCBs inhibited adipocyte differentiation and the expressions of CCAAT/enhancer binding proteins (C/EBPs) and peroxisome proliferators-activated receptor γ (PPARγ). Cf-LCBs increased β-catenin mRNA and nuclear translocation and increased its target genes, cyclin D1 and c-myc. Cf-LCBs enhanced fizzled and lipoprotein-receptor-related protein5/6 (LRP5/6) expressions, whereas wingless-type MMTV integration site10b (WNT10b) and glycogen syntheses kinase 3β (GSK3β) remained unchanged. Cf-LCBs also reduced adipogenesis and recovered WNT/β-catenin signaling in the cells suffering from 21H7, a β-catenin inhibitor. In addition, Cf-LCBs decreased triglyceride content and the expressions of lipogenesis genes. Cf-LCBs increased FFA levels and the expressions of lipidolytic factors. Cf-LCBs promoted the phosphorylation of adenosinemonophosphate- activated protein kinase (AMPK) and acetyl-CoA carboxylase. These findings indicate that Cf-LCBs inhibit adipogenesis through activation of WNT/β-catenin signaling and regulate lipid metabolism via activation of AMPK pathway.

A XSS Attack Detection Method based on Skip List

Shan Chun,Cui Jing,Hu ChangZhen,Xue JingFeng,Wang Hao,Mhagama Raphael 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.5

Dietary Saponins of Sea Cucumber Ameliorate Obesity, Hepatic Steatosis, and Glucose Intolerance in High-Fat Diet–Fed Mice

Xiaoqian Hu,Zhaojie Li,Yong Xue,Jingfeng Wang,Yuming Wang 한국식품영양과학회 2012 Journal of medicinal food Vol.15 No.10

Much attention has been focused on food components that may be beneficial in preventing lifestyle-related diseases. In this study, we investigated the effects of saponins of sea cucumber (SSC) on high-fat diet–induced obesity, insulin resistance, and fatty liver in mice. C57/BL6 mice were fed a high-fat diet, containing 0.03% SSC, or 0.1% SSC for 8 weeks. Both doses of SSC exhibited a weight-loss effect and significantly decreased adipose tissue weight, in both visceral and subcutaneous depots. Furthermore, 0.1% SSC treatment dramatically decreased the hepatic triglyceride and total cholesterol accumulation. Mice administrated with 0.1% SSC had significantly decreased serum glucose and insulin levels, lower homeostatic model assessment for insulin resistance index, and area under the blood glucose curve, suggesting that insulin sensitivity is enhanced by dietary SSC. Dietary SSC also prevented adipokine imbalance, by increasing adiponectin production and decreasing tumor necrosis factor alpha level caused by high-fat diet. Overall, these data demonstrate that SSC could improve certain metabolic parameters associated with obesity.

Long-chain bases from Cucumaria frondosa inhibit adipogenesis and regulate lipid metabolism in 3T3-L1 adipocytes

Tian, Yingying,Hu, Shiwei,Xu, Hui,Wang, Jingfeng,Xue, Changhu,Wang, Yuming 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.6

This study aims to investigate anti-adipogenic effects of long-chain bases from Cucumaria frondosa (Cf-LCBs) in vitro. Results showed that Cf-LCBs inhibited adipocyte differentiation and the expressions of CCAAT/enhancer binding proteins (C/EBPs) and peroxisome proliferators-activated receptor ${\gamma}(PPAR{\gamma})$. Cf-LCBs increased ${\beta}-catenin$ mRNA and nuclear translocation and increased its target genes, cyclin D1 and c-myc. Cf-LCBs enhanced fizzled and lipoprotein-receptor-related protein5/6 (LRP5/6) expressions, whereas wingless-type MMTV integration site10b (WNT10b) and glycogen syntheses kinase $3{\beta}(GSK3{\beta})$ remained unchanged. Cf-LCBs also reduced adipogenesis and recovered WNT/${\beta}-catenin$ signaling in the cells suffering from 21H7, a ${\beta}-catenin$ inhibitor. In addition, Cf-LCBs decreased triglyceride content and the expressions of lipogenesis genes. Cf-LCBs increased FFA levels and the expressions of lipidolytic factors. Cf-LCBs promoted the phosphorylation of adenosinemonophosphate-activated protein kinase (AMPK) and acetyl-CoA carboxylase. These findings indicate that Cf-LCBs inhibit adipogenesis through activation of WNT/${\beta}-catenin$ signaling and regulate lipid metabolism via activation of AMPK pathway.

Oil from Antarctic krill (Euphausia superba) facilitates bone formation in dexamethasone-treated mice

Lei Mao,Fei Wang,Yuanyuan Li,Yufeng Dai,Yanjun Liu,Jingfeng Wang,Changhu Xue 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.2

Glucocorticoids are the leading cause of secondaryosteoporosis. In the current study, the in vivoeffects of Antarctic krill (Euphausia superba) oil (AKO) ondexamethasone-treated mice were investigated. Resultsshowed that AKO significantly prevents bone loss, asevidenced by improved bone mineral density, biomechanicalstrength, and cancellous bone microstructure. Fluorescencedouble-labeling of femur showed that AKOinduces new bone formation. Toluidine blue staining ofmarrow cavity indicated that AKO increases the number oftrabecula, and decreases the generation of adipose cells. Runt-related transcription factor 2 (Runx2) and Peroxisomeproliferator-activated receptor c (PPARc) are the switchesfor osteogenic and adipogenic differentiation of bonemarrow mesenchymal stem cells, respectively. AKO significantlypromoted the expression of Runx2 protein, andreduced PPARc expression in bone tissue. Furthermore,AKO increased the mRNA expression of osteogenesis-relatedgenes and decreased the expression of adipogenesisrelatedgenes. In conclusion, AKO improved glucocorticoid-induced osteoporosis via promoting bone formation.

DHA-enriched phosphatidylcholine suppressed angiogenesis by activating PPARγ and modulating the VEGFR2/Ras/ERK pathway in human umbilical vein endothelial cells

Yuanyuan Liu,Yingying Tian,Yao Guo,Ziyi Yan,Changhu Xue,Jingfeng Wang 한국식품과학회 2021 Food Science and Biotechnology Vol.30 No.12

Docosahexaenoic acid-enriched phosphatidylcholine(DHA-PC) is a new generation of omega-3 lipids,which contains an ester bond linking DHA at the sn-2position of phospholipid. DHA-PC has become the interestrecently as its better bioavailability and anti-oxidationcapacity. In this study, the anti-angiogenic effect of DHAPCwas evaluated. The capacities of proliferation, migration,tube formation of human umbilical vein endothelialcells were significantly declined after DHA-PC treatment. Furthermore, DHA-PC inhibited the neovascularization ofthe chick chorioallantoic membrane in vivo. Mechanismresults indicated that DHA-PC enhances the expression ofperoxisome proliferator-activated receptor c (PPARc) attranscriptional and translational level, subsequently downregulatesthe VEGFR2 expression and VEGFR2-mediateddownstream Ras/ERK pathway, resulting in significantreduction in proliferation and differentiation. Additionally,PPARc-specific antagonist GW9662 partly reversed theinhibition effects of DHA-PC on tube formation and neovascularization,suggesting that DHA-PC exerts anti-angiogenesiseffect through activating PPARc. Thesefindings indicated that DHA-PC has a great prospect ofanti-tumor angiogenesis therapy.