Fibulin2: a negative regulator of BMSC osteogenic differentiation in infected bone fracture healing

Li Shi-Dan,Xing Wei,Wang Shao-Chuan,Li You-Bin,Jiang Hao,Zheng Han-Xuan,Li Xiao-Ming,Yang Jing,Guo De-Bin,Xie Xiao-Yu,Jiang Ren-Qing,Fan Chao,Li Lei,Xu Xiang,Fei Jun 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Bone fracture remains a common occurrence, with a population-weighted incidence of approximately 3.21 per 1000. In addition, approximately 2% to 50% of patients with skeletal fractures will develop an infection, one of the causes of disordered bone healing. Dysfunction of bone marrow mesenchymal stem cells (BMSCs) plays a key role in disordered bone repair. However, the specific mechanisms underlying BMSC dysfunction caused by bone infection are largely unknown. In this study, we discovered that Fibulin2 expression was upregulated in infected bone tissues and that BMSCs were the source of infection-induced Fibulin2. Importantly, Fibulin2 knockout accelerated mineralized bone formation during skeletal development and inhibited inflammatory bone resorption. We demonstrated that Fibulin2 suppressed BMSC osteogenic differentiation by binding to Notch2 and inactivating the Notch2 signaling pathway. Moreover, Fibulin2 knockdown restored Notch2 pathway activation and promoted BMSC osteogenesis; these outcomes were abolished by DAPT, a Notch inhibitor. Furthermore, transplanted Fibulin2 knockdown BMSCs displayed better bone repair potential in vivo. Altogether, Fibulin2 is a negative regulator of BMSC osteogenic differentiation that inhibits osteogenesis by inactivating the Notch2 signaling pathway in infected bone.

Crashworthy and Performance-cost Characteristics of Aluminum-CFRP Hybrid Tubes under Quasi-static Axial Loading

Peilong Shi,Qiang Yu,Rong Huang,Xuan Zhao,Guohua Zhu 한국섬유공학회 2019 Fibers and polymers Vol.20 No.2

Metal/composite hybrid thin-walled structures combine the low cost of metallic materials and the high strength-toweight ratio of composites and thus have the potential to be utilized as cost-effective energy absorbers for vehicle applications. This study aimed to examine the crushing behaviors and performance-to-cost ratio of aluminum/carbon fiberreinforced plastic (CFRP) hybrid tubes under quasi-static axial loading. First, a single aluminum tube, a single CFRP tube and an aluminum/CFRP hybrid tube were tested to validate numerical models. The experimental results showed that the total energy absorption (EA) of the aluminum/CFRP hybrid tube was 32.46 % higher than the sum of that of the individual parts, and the special energy absorption (SEA) of the hybrid tube was improved by 105.26 % compared with that of the single aluminum tube. Then, the effects of the orientation angles ([±15 o]n, [±45 o]n, [±75 o]n, [±90 o]n, [90 o/0 o]n, n=2, 4, 6) and thicknesses of the CFRP tube wall (4-ply, 8-ply, 12-ply) on the crashworthiness of the hybrid tube were studied through validated numerical models. The numerical results showed that as the orientation angle increased, SEA, EA and the mean crushing force (Fmean) decreased first and then increased; in addition, the hybrid tubes with orientation angles of [±45 o]n and [90 o/0 o]n (n=2, 4, 6) consistently exhibited the worst and best crashworthiness, respectively. Furthermore, the SEA, EA, and Fmean of the hybrid tube increased with increasing thickness of the CFRP tube wall. Finally, the performance-to-cost ratio (SEA/cost) of the hybrid tube was analyzed, and the results show that aluminum/CFRP hybrid tubes with a smaller wall thickness of the CFRP tube exhibits superior potential in terms of both cost and performance for automotive applications.

Research on Factors Influencing Chinese Consumers' Intention to Buy Agricultural Fresh Products Online - Evidence from Tangshan City

Shuang-yu Hu,Shi-yong Piao,Zhi-run Li,Yu-cong Sun,Xuan-you Jin,Jong-In Lee 한국국제농업개발학회 2020 韓國國際農業開發學會誌 Vol.32 No.4

This paper is based on field survey data and applies it to the logistic model to analyze consumers' online shopping intention to buy fresh produce. According to the total effect of online shopping intention, it was revealed that consumers' age, educational level, and monthly household income significantly influence willingness to buy fresh produce online. The product quality, price and brand name were the main factors affecting consumers' willingness to buy fresh produce online. The customer service quality, payment security, and logistics quality significantly also affected consumers' willingness to buy fresh produce online. In conclusion, it is important for vitalizing online sales of fresh produce to ensure the quality, use well-known brand name, and improve logistics service and e-commerce platforms.

A Study of the Regional Brand Recognition of Yanbian Cattle in China and the Resulting Effect on Consumers' Purchasing Intention Likelihood

Shuang-yu Hu,Shi-yong Piao,Zhi-run Li,Yu-cong Sun,Xuan-you Jin,Jong-In Lee 한국국제농업개발학회 2021 韓國國際農業開發學會誌 Vol.33 No.1

본 연구에서는 중국 길림성 연변 주 지역의 ‘연변황우’ 소비자 대상으로 설문조사를 실시하였음. 먼저 국내외 브랜드 인지 및 구매 의사에 관한 이론을 검토하고 소비자의 브랜드 인식 등의 이론을 바탕으로 연구 가설을 제시하였음. ‘연변황우’에 대한 소비자 구매 의사에 영향을 미치는 요인을 구축하여 분석을 실시하였음. 연구 결과 1. ‘연변황우’ 브랜드 이미지는 소비자의 구매 의사에 긍정적인 영향을 미치며 소비자의 ‘품질 감지’ 요인에 정(+)의 영향을 미침. 2. ‘위험 감지’ 요인에는 부(-)의 영향을 미침. ‘품질 감지’ 요인은 ‘연변황우’ 구매 의사에 긍정적인 영향을 미침. 3. ‘연변황우’의 브랜드 이미지 관리를 강화해야하며 이는 브랜드의 연구관련에 새로운 분야 탐색에 중요한 의의가 있음. This paper summarizes both domestic and foreign research theories centered around consumers' relationships to brand recognition and consumption patterns, and focuses on the results of a field survey conducted among a group of consumers in Yanbian. With the use of a variety of theories, including consumer brand equity theory, we present different research hypotheses which give insight into the different factors which affect the Yanbian Cattle's brand recognition in different regional contexts. Through empirical analysis, we measured the strength of the model which linked Yanbian Yellow Cattle's regional brand recognition with consumers' purchasing intentions. Based on the results of the study, we were able to fully analyze the future directions of the product's brand recognition, basing this analysis on a deep understanding of the regional and industrial environment of the Yanbian. With these results, we bring forward suggestions for the management of the Yanbian Yellow Cattle's regional branding strategy, and offer insights into new future research directions that would more fully support unique and effective corporate branding strategies.

Study of Factors Affecting Consumers’ Willingness to Pay for Certified Traceable Pork in Yanbian

Shuang-Yu HU,Shi-Yong Piao,Zhi-Run Li,손우총,Xuan-You Jin,이종인 경상국립대학교 농업생명과학연구원 2021 농업생명과학연구 Vol.55 No.3

With the development of the economy and the continuous improvement of people’s living standards, consumers’ increasing demand for safe food has become an inevitable trend of social development. On the one hand, food safety is an urgent social problem needed to be solved in China. On the other hand, the food safety guarantee method based on a traceability system is popular. This paper systematically studies the influencing factors of consumers’ willingness to pay for the extra price of traceable pork in the Yanbian area through empirical analysis. The result showed that consumers’ attitude and subjective norms, and perceived behavior control, have significantly positively affect consumers’ willingness to pay the extra price. Meanwhile, consumers’ perceived risk of traceable pork significantly moderates the effect on consumers’ attitude and willingness to pay an extra price. The results can provide the corresponding reference for government agencies to formulate policies and provide valuable suggestions for enterprises to develop a pork traceability market to realize the development of a pork traceability system in the Yanbian area to ensure consumers’ food safety.

Autophagy Inhibition Promotes Gambogic Acid-induced Suppression of Growth and Apoptosis in Glioblastoma Cells

Luo, Guo-Xuan,Cai, Jun,Lin, Jing-Zhi,Luo, Wei-Shi,Luo, Heng-Shan,Jiang, Yu-Yang,Zhang, Yong Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12

Objective: To investigate the effects of gambogic acid (GA) on the growth of human malignant glioma cells. Methods: U251MG and U87MG human glioma cell lines were treated with GA and growth and proliferation were investigated by MTT and colony formation assays. Cell apoptosis was analyzed by annexin V FITC/PI flow cytometry, mitochondrial membrane potential assays and DAPI nuclear staining. Monodansylcadaverine (MDC) staining and GFP-LC3 localisation were used to detect autophagy. Western blotting was used to investigate the molecular changes that occurred in the course of GA treatment. Results: GA treatment significantly suppressed cell proliferation and colony formation, induced apoptosis in U251 and U87MG glioblastoma cells in a time- and dose-dependent manner. GA treatment also lead to the accumulation of monodansylcadaverine (MDC) in autophagic vacuoles, upregulated expressions of Atg5, Beclin 1 and LC3-II, and the increase of punctate fluorescent signals in glioblastoma cells pre-transfected with GFP-tagged LC3 plasmid. After the combination treatment of autophagy inhitors and GA, GA mediated growth inhibition and apoptotic cell death was further potentiated. Conclusion: Our results suggested that autophagic responses play roles as a self-protective mechanism in GA-treated glioblastoma cells, and autophagy inhibition could be a novel adjunctive strategy for enhancing chemotherapeutic effect of GA as an anti-malignant glioma agent.

Research on Hydrostatic Thrust Bearing Worktable Job Status

Jian-yi Zhang,Xuan-Yu Shi,Gui-Hua Han 보안공학연구지원센터 2014 International Journal of Smart Home Vol.8 No.1

Arabidopsis AMY1 expressions and early flowering mutant phenotype

( Wang Jie ),( Da Shi Yu ),( Xin Hong Guo ),( Xuan Ming Liu ) 생화학분자생물학회 2009 BMB Reports Vol.42 No.2

Analysis of Operating Efficiency of China’s Agricultural Listed Companies

Zhi-Run Li,Shi-Yong Piao,Yu-Cong Sun,Shuang-Yu HU,Xuan-You Jin,Jong-In Lee 경상대학교 농업생명과학연구원 2020 농업생명과학연구 Vol.54 No.6

In recent years, China's agricultural listed companies have developed rapidly. This paper studies the operating efficiency of listed agricultural companies in China. exploring the factors that affect the operating efficiency of listed agricultural companies, and proposes targeted countermeasures for the development of listed agricultural companies. And give some suggestions to Korean agricultural companies and A share investors. This paper uses the DEA model, selects the 40 best-developed Chinese agricultural listed companies in 2018 as a sample, analyzes the operating efficiency of these companies, and added two new input variables: asset impairment losses (AIL) and business tax and surcharge (BTS), which will also have an impact on operating efficiency. This paper can provide reliable suggestions for the development of agricultural listed companies, and thus guide the healthy operation of agricultural companies.

Investigation on the interactions of scutellarin and scutellarein with bovine serum albumin using spectroscopic and molecular docking techniques

Hao Tang,Nian-Guang Li,Zhi-Hao Shi,Yu-Ping Tang,Qian-Ping Shi,Ze-Xi Dong,Peng-Xuan Zhang,Jin-ao Duan 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.10

The binding abilities of scutellarin (Scu) andscutellarein (Scue) with bovine serum albumin (BSA) wereinvestigated using equilibrium dialysis, high performanceliquid chromatography, fluorescence spectroscopy, competitivesite marker and molecular docking. The resultsshowed that the average protein binding ratios of Scu andScue with BSA were (79.85 ± 1.83) and (85.49 ± 1.21) %respectively. Under simulated physiological conditions, thefluorescence data indicated that Scu and Scue bound withBSA through a static mechanism. The thermodynamicparameters indicated that the interactions of Scu-BSA andScue-BSA mainly occurred by van der Waals forces andhydrogen bonds and it was easier for Scue to bind withBSA than Scu, indicating that the glucuronic acid moleculein Scu decreased the binding affinity. Site competitivemarker experiments showed that the binding sites of Scuand Scue mainly located within the sub-domain IIA ofBSA. Furthermore, molecular docking studies indicatedthat one BSA could bind three Scue, while one BSA couldcarry only two Scu. All these results clearly indicated theinteractions of Scu and Scue with BSA, which will lay thefoundation for further research to determine the pharmacologyand pharmacodynamics of Scu and Scue for treatingischemic cerebrovascular disease.