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Xu, Jian-Yu,Lu, Shan,Xu, Xiang-Ying,Hu, Song-Liu,Li, Bin,Qi, Rui-Xue,Chen, Lin,Chang, Joe Y. Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.8
Nucleolin (C23) is an important anti-apoptotic protein that is ubiquitously expressed in exponentially growing eukaryotic cells. In order to understand the impact of C23 in radiation therapy, we attempted to investigate the relationship of C23 expression with the radiosensitivity of human non-small cell lung cancer (NSCLC) cells. We investigated the role of C23 in activating the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs), which is a critical protein for DNA double-strand breaks (DSBs) repair. As a result, we found that the expression of C23 was negatively correlated with the radiosensitivity of NSCLC cell lines. In vitro clonogenic survival assays revealed that C23 knockdown increased the radiosensitivity of a human lung adenocarcinoma cell line, potentially through the promotion of radiation-induced apoptosis and adjusting the cell cycle to a more radiosensitive stage. Immunofluorescence data revealed an increasing quantity of ${gamma}$-H2AX foci and decreasing radiation-induced DNA damage repair following knockdown of C23. To further clarify the mechanism of C23 in DNA DSBs repair, we detected the expression of DNA-PKcs and C23 proteins in NSCLC cell lines. C23 might participate in DNA DSBs repair for the reason that the expression of DNA-PKcs decreased at 30, 60, 120 and 360 minutes after irradiation in C23 knockdown cells. Especially, the activity of DNA-PKcs phosphorylation sites at the S2056 and T2609 was significantly suppressed. Therefore we concluded that C23 knockdown can inhibit DNA-PKcs phosphorylation activity at the S2056 and T2609 sites, thus reducing the radiation damage repair and increasing the radiosensitivity of NSCLC cells. Taken together, the inhibition of C23 expression was shown to increase the radiosensitivity of NSCLC cells, as implied by the relevance to the notably decreased DNA-PKcs phosphorylation activity at the S2056 and T2609 clusters. Further research on targeted C23 treatment may promote effectiveness of radiotherapy and provide new targets for NSCLC patients.
Yu-fang Zheng,Liang-liang Xu,Chang-ping Chen 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.3
Nonlinear bending analysis was performed of magnetoelectroelastic (MEE) composite plates under a mechanical and magnetoelectric (ME) load by using von Karman’s nonlinear geometric equation and the higher order shear deformation theory (HSDT). Nonlinear higher order partial differential equations for MEE plates were derived by using Hamiltonian equilibrium equation. The MEE plate is considered to have clamped boundary condition. The nonlinear high-order equations can turn into algebraic equations through Galerkin method. Then the effects of scale effect of MEE plate (for instance, the aspect ratio) and external load (for instance, mechanical) on the displacement of the considered MEE plate were investigated.
Xu, Shu-Sheng,Weng, Li-Jun,Liu, Yu-Zhen,Kang, Kyeong-Hee,Kim, Chang-Lae,Kim, Dae-Eun Elsevier Sequoia 2017 Surface & coatings technology Vol.325 No.-
<P><B>Abstract</B></P> <P>Ni-doped WS<SUB>2</SUB> composite coatings with various Ni contents were co-deposited using a radio frequency sputtering system on silicon wafer and AISI 440C stainless steel substrates. The microstructural characteristics of the WS<SUB>2</SUB>-Ni composite coatings and their tribological properties in vacuum were assessed. During introduction of Ni dopant in the WS<SUB>2</SUB>-Ni composite coating the sulfur/tungsten (S/W) ratio in the coating increased due to reduced preferential resputtering of sulfur atoms in the growing coating. The microstructure of the WS<SUB>2</SUB>-Ni composite coating varied from a fine columnar structure for Ni content equal to or less than 7.7at.% to a featureless structure as the Ni content increased further. The Ni dopant inhibited the growth of the coarse columnar WS<SUB>2</SUB> platelets which was accompanied by nanocrystallization and amorphization of the composite coating structure. WS<SUB>2</SUB>-Ni composite coatings with fine columnar structure exhibited relatively low hardness but showed a high tendency to form a lubricating transfer layer. It also demonstrated low brittleness and prolonged wear life in vacuum condition compared to coatings with dense featureless structure. The variation in tribological performance between the composite coatings resulted from the different wear mechanisms associated with their distinct microstructures.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Microstructure of WS<SUB>2</SUB> coating was modified by incorporating Ni as a dopant. </LI> <LI> The introduction of Ni increased the S/W ratio of the WS<SUB>2</SUB> composite coating. </LI> <LI> High Ni content in the WS<SUB>2</SUB> coating led to high brittleness and low wear resistance. </LI> <LI> WS<SUB>2</SUB>–5at.% Ni coating showed 5 times longer wear life than pure WS<SUB>2</SUB> film in vacuum. </LI> <LI> Superior tribological properties were attributed to transfer layer and high hardness. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Xu, Ying,Tian, Xin-Peng,Liu, Yu-Juan,Li, Jie,Kim, Chang-Jin,Yin, Hao,Li, Wen-Jun,Zhang, Si International Union of Microbiological Societies 2013 International journal of systematic and evolutiona Vol.63 No.3
<P>A marine bacterium, designated SCSIO 03483<SUP>T</SUP>, was isolated from a marine sediment sample collected from the Nansha Islands in the South China Sea. The strain produced roundish colonies with diffusible yellow-coloured pigment on nutrient agar medium or marine agar 2216. Optimal growth occurred in the presence of 0–4 % (w/v) NaCl, at pH 7.0 and a temperature range of 28–37 °C. 16S rRNA gene sequence analysis indicated that the isolate belonged to the family <I>Flavobacteriaceae</I> and showed relatively high sequence similarity with <I>Imtechella halotolerans</I> K1<SUP>T</SUP> (92.7 %). Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the isolate shared a lineage with members of the genera <I>Imtechella</I>, <I>Joostella</I> and <I>Zhouia</I>. Phospholipids were phosphatidylethanolamine, two unidentified aminolipids and three unknown polar lipids. The major respiratory quinone was MK-6 and the major fatty acids were iso-C<SUB>15 : 0</SUB>, iso-C<SUB>17 : 0</SUB> 3-OH and summed feature 3 (C<SUB>16 : 1</SUB>ω6<I>c</I>/C<SUB>16 : 1</SUB>ω7<I>c</I>). The DNA G+C content of strain SCSIO 03483<SUP>T</SUP> was 38.4 mol%. On the basis of phenotypic, chemotaxonomic and molecular data, strain SCSIO 03483<SUP>T</SUP> represents a novel species in a new genus in the family <I>Flavobacteriaceae</I>, for which the name <I>Sinomicrobium oceani</I> gen. nov., sp. nov. is proposed. The type strain of <I>Sinobacterium oceani</I> is SCSIO 03483<SUP>T</SUP> ( = KCTC 23994<SUP>T</SUP> = CGMCC 1.12145<SUP>T</SUP>).</P>
Zhao, Chang,Wang, Xu-Jie,Wang, Song,Feng, Wei-Hua,Shi, Lei,Yu, Chun-Peng Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.5
Purpose: To assess the effect and safety of lobaplatin combinated floxuridine /pirarubicin in transcatheter hepatic arterial chemoembolization(TACE) of unresectable primary liver cancer. Patients and Methods: TACE combined with the chemotherapy regimen was used to treat 34 unresectable primary liver cancer patients. DSA/MRI/CT/blood routine examinations were used to evaluate short term activity and toxicity after 4-5 weeks, the process being repeated if necessary. Results: Among the 34 cases, 1 (2.9%) showed a complete response, 21 (61.7%) a partial response, 8 (23.5%) stable disease, and 4 progressive disease, with a total effective rate of 67.6%. The content of alpha fetoprotein dropped by over 50% in 20 cases (58.8%). The rate of recovery was hepatalgia (88.2%), ascites (47.1%), appetite (55.9%), Performance Status(30.4%). The median follow-up time (MFT) was 281 days (63-558 days), and median progression-free survival was 118.5 days (95%, CI:88.8-148.2days). Adverse reactions (III-IV grade) were not common, with only 4 cases of vomiting and 2 cases of thrombocytopenia (III grade). Conclusions: Lobaplatin-based TACE is an effective and safe treatment for primary liver cancer.
Guo, Yu,Xu, Li-Sha,Zhang, Ding,Liao, Ya-Ping,Wang, Hai-ping,Lan, Zhi-Hui,Guan, Wei-Jun,Liu, Chang-Qing Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.8
Objectives: To investigate the effects of betaine on HeLa cell growth and apoptosis and molecular mechanisms. Materials and Methods: Concentrations of 0.1, 1.0, 5.0, 20.0, 100.0 mg/ml of betaine were used to evaluate the anticancer efficacy for HeLa cells respectively, and MCF-10A was also detected as a normal diploid cell control. Results: We found that proliferation of HeLa cells was inhibited significantly upon exposure to increasing betaine levels with the MTT test (p<0.05). The percentage of S phase cells in the low dose groups (<5mg/ml) were distinctly higher than in high dose groups, and the rates of Sub-G1 phase were the opposite (p<0.01); A high concentration of betaine (>5.0mg/ml) significantly promoted the apoptosis of HeLa cells (p<0.01). SOD activities of the low dose groups were slightly higher than the control group (p<0.05) and there were obvious synchronicity and correlation among the expression of promoting apoptosis genes Bax, P53, Caspase 3 and apoptosis suppression gene Bcl-2. In response to an apoptosis-inducing stimulus, p53 and cyclin D1 could be activated with blockage of the cell cycle at G1/S or S/G2 checkpoints. Conclusions: Our data showed that betaine could promote HeLa cells proliferation in vitro at low concentrations. In contrast, high concentrations could significantly inhibit cell growth and migration, and induce apoptosis of HeLa cells through caspase 3 signaling and further promoted necrosis. This might imply that betaine exhibits tumoricidal effects and acts as a biological response modifier in cancer treatment by inducing apoptosis and cell cycle arrest in a dose and time-dependent manner.
Guo, Yu,Liao, Ya-Ping,Zhang, Ding,Xu, Li-Sha,Li, Na,Guan, Wei-Jun,Liu, Chang-Qing Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.5
Although nucleolar protein nucleostemin (NS) is essential for cell proliferation and early embryogenesis and expression has been observed in some types of human cancer and stem cells, the molecular mechanisms involved in mediation of cell proliferation and cell cycling remains largely elusive. The aim of the present study was to evaluate NS as a potential target for gene therapy of human breast carcinoma by investigating NS gene expression and its effects on SKBR-3 cell proliferation and apoptosis. NS mRNA and protein were both found to be highly expressed in all detected cancer cell lines. The apoptotic rate of the pcDNA3.1-NS-Silencer group ($12.1-15.4{\pm}3.8%$) was significantly higher than those of pcDNA3.1-NS ($7.2-12.0{\pm}1.7%$) and non-transfection groups ($4.1-6.5{\pm}1.8%$, P<0.01). MTT assays showed the knockdown of NS expression reduced the proliferation rate of SKBR-3 cells significantly. Matrigel invasion and wound healing assays indicated that the number of invading cells was significantly decreased in the pcDNA3.1-NS-siRNA group (P<0.01), but there were no significant difference between non-transfected and over-expression groups (P>0.05). Moreover, RNAi-mediated NS down-regulation induced SKBR-3 cell G1 phase arrest, inhibited cell proliferation, and promoted p53 pathway-mediated cell apoptosis in SKBR-3 cells. NS might thus be an important regulator in the G2/M check point of cell cycle, blocking SKBR-3 cell progression through the G1/S phase. On the whole, these results suggest NS might be a tumor suppressor and important therapeutic target in human cancers.
Qian Yu,Bin Tang,Chang Huang,Yadong Wei,Shaojia Chen,Lin Qiu,Xiuku Wang,Hong Xu,Zhijia Sun,Guangyou Wei,Mengjiao Tang 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.3
A high detection efficiency thermal neutron detector based on the 6LiF/ZnS(Ag) scintillation screens,wavelength-shifting fibers (WLSF) and Silicon photomultiplier (SiPM) readout is under development atChina Spallation Neutron Source (CSNS) for the Engineering Material Diffractometer (EMD).A prototypewith a sensitive volume of 180mm 192mm has been built. Signals from SiPMs are processed by the selfdesignApplication Specific Integrated Circuit (ASIC). The performances of this detector prototype are as follows: neutron detection efficiency could reach50.5% at 1 Å, position resolution of 3, the dark count rate <0.1Hz, the maximum count rate >200KHz. Such detector prototype could be an elementary unit for applications in the EMD detector arrays.