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      • KCI등재
      • KCI등재

        Comparative Metabolite Profiling and Hormone Analysis of Perennial and Annual Rice

        Xiuqin Zhao,Ting Zhang,Liyu Huang,Huimin Wu,Fengyi Hu,Fan Zhang,Linghua Zhu,Binying Fu 한국식물학회 2012 Journal of Plant Biology Vol.55 No.1

        Perenniality is one of the important topics in rice breeding which is generally accompanied by complex physiobiochemical processes. To understand the metabolic characteristics of perennial rice, in the present study, gas chromatography–mass spectrometry and enzyme-linked immunosorbent assays were used to profile the distribution patterns of 33 primary metabolites and hormones [indole-3-acetic acid (IAA) and zeatin riboside (ZR)] of annual (RD23) and perennial (Oryza longistaminata and the line AA with RD23 genetic background) rice genotypes. Results showed that both metabolites and hormones have distinct genotype and organ distribution patterns, and considerable variations were observed between the metabolites in stem bases of perennial and annual rice. Most of the metabolites,including sugars, organic acids, and amino acids, significantly accumulated in the stem bases of perennial rice by decreasing the level in roots and leaves. Fifteen metabolites consistently accumulated significantly in the stem bases of both perennial genotypes. Additionally, the organ-level IAA content and IAA/ZR ratio in the two perennials were considerably higher than those in RD23. The present study indicated that the significant accumulation of the metabolites at stem base and the higher IAA/ZR ratio are involved

      • KCI등재

        Fast FCS-MPC-Based SVPWM Method to Reduce Switching States of Multilevel Cascaded H-Bridge STATCOMs

        Xiuqin Wang,Jiwen Zhao,Qunjing Wang,Guoli Li,Maosong Zhang 전력전자학회 2019 JOURNAL OF POWER ELECTRONICS Vol.19 No.1

        Finite control set model-predictive control (FCS-MPC) has received increasing attentions due to its outstanding dynamicperformance. It is being widely used in power converters and multilevel inverters. However, FCS-MPC requires a lot ofcalculations, especially for multilevel-cascaded H-bridge (CHB) static synchronous compensators (STATCOMs), since it has totake account of all the feasible voltage vectors of inverters. Hence, an improved five-segment space vector pulse widthmodulation (SVPWM) method based on the non-orthogonal static reference frames is proposed. The proposed SVPWM methodhas a lower number of switching states and requires fewer computations than the conventional method. As a result, it makesFCS-MPC more efficient for multilevel cascaded H-bridge STATCOMs. The partial cost function is adopted to sequentiallysolve for the reference current and capacitor voltage. The proposed FCS-MPC method can reduce the calculation burden of theFCS-MPC strategy, and reduce both the switching frequency and power losses. Simulation and experimental results validate theexcellent performance of the proposed method when compared with the conventional approach.

      • KCI등재

        Analysis of ESTs from a Normalized cDNA Library of the Rhizome Tip of Oryza longistaminata

        Ting Zhang,Lijuan Li,Fengyi Hu,Xiuqin Zhao,Binying Fu,Daichang Yang 한국식물학회 2012 Journal of Plant Biology Vol.55 No.1

        Oryza longistaminata, a perennial wild rice species with an AA genome, is characterized by the presence of rhizomatous stems. The rhizomatous trait in rice was previously shown to be quantitatively controlled by many genes, but the molecular mechanism related to rhizome development is still unknown. In the present study, expressed sequence tags (ESTs) generated from rhizome tips of O. longistaminata were collected and analyzed. A total of 10,283 complimentary deoxyribunucleic acid clones were randomly sequenced, which generated 10,136 raw sequences,and finally, 4,419 unisequences with diverse functional categories were generated. These unisequences were mapped onto the Oryza sativa genome, which revealed that 4,285(96.97%) and 4,151 (93.94%) of the unisequences were alignable to the japonica and indica genomic sequences,respectively, with >80% sequence identity. Additionally, 41unisequences showed four typical types of alternative splicing patterns. More than 600 simple sequence repeats were identified in these unisequences. A subset of unisequences were physically colocalized onto rhizome-related quantitative trait locus intervals in rice and sorghum, and one gene,OLRR1, was further confirmed to be enriched in the rhizome tip and young leaf by real-time polymerase chain reaction and in situ hybridization. Unisequences reported in this study provide valuable data for molecular dissection of the rhizomatous growth habit in O. longistaminata.

      • SCIESCOPUSKCI등재

        Fast FCS-MPC-Based SVPWM Method to Reduce Switching States of Multilevel Cascaded H-Bridge STATCOMs

        Wang, Xiuqin,Zhao, Jiwen,Wang, Qunjing,Li, Guoli,Zhang, Maosong The Korean Institute of Power Electronics 2019 JOURNAL OF POWER ELECTRONICS Vol.19 No.1

        Finite control set model-predictive control (FCS-MPC) has received increasing attentions due to its outstanding dynamic performance. It is being widely used in power converters and multilevel inverters. However, FCS-MPC requires a lot of calculations, especially for multilevel-cascaded H-bridge (CHB) static synchronous compensators (STATCOMs), since it has to take account of all the feasible voltage vectors of inverters. Hence, an improved five-segment space vector pulse width modulation (SVPWM) method based on the non-orthogonal static reference frames is proposed. The proposed SVPWM method has a lower number of switching states and requires fewer computations than the conventional method. As a result, it makes FCS-MPC more efficient for multilevel cascaded H-bridge STATCOMs. The partial cost function is adopted to sequentially solve for the reference current and capacitor voltage. The proposed FCS-MPC method can reduce the calculation burden of the FCS-MPC strategy, and reduce both the switching frequency and power losses. Simulation and experimental results validate the excellent performance of the proposed method when compared with the conventional approach.

      • KCI등재

        Preparation and properties of Al-Al2O3 metal ceramics via powder metallurgy methods

        Jieguang Song,Xiuqin Wang,Lin Chen,Cailiang Pang,Jia Zhang,Weiguo Shi,Songlin Guo,Xianzhong Wang,Ruihua Wang 한양대학교 세라믹연구소 2018 Journal of Ceramic Processing Research Vol.19 No.1

        Metal ceramics are outstanding new engineering materials that offer the advantages of both ceramics and metals. In this study,Al-Al2O3 metal ceramics were prepared through powder metallurgy methods, and the densification and properties of theprepared metal ceramics were investigated to gain insights into the preparation of high-performance cermet materials. Resultsrevealed that as the forming pressure was increased, the relative density of the ceramics increased, whereas their electricalresistivity decreased. The relative density increased with increasing sintering temperature up to 700 oC and decreased beyond700 oC, whereas the electrical resistivity exhibited an opposite trend. The relative density increased with increasing Al content,whereas the electrical resistivity displayed an opposite trend. Under optimal technological parameters of 700 oC sinteringtemperature, < 20MPa forming pressure, and 75 wt% Al content, Al-Al2O3 metal ceramics with a high relative density(97.52%) and a low electrical resistivity (101.34 Ω·m) were successfully prepared.

      • KCI등재

        Synthesisof Fructose Laurate Esters Catalyzed by a CALB-displaying Pichia pastoris Whole-cell Biocatalyst in a Non-aqueous System

        Zi Jin,Shuli Liang,Xiuqin Zhang,Shuangyan Han,Changqiong Ren,Ying Lin,Suiping Zheng 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.2

        Earlier studies on fructose laurate ester products have shown that recombinant Pichia pastoris displaying Candida antarctica lipase B (CALB) on the cell surface acts as an efficient whole-cell biocatalyst for sugar ester production from fructose and lauric acid in an organic solvent. The effects of various reaction factors, including solvent composition, substrate molar ratio, enzyme dose,temperature and water activity, on esterification catalyzed by the CALB-displaying P. pastoris whole-cell biocatalyst were examined in the present study. Under the preferred reaction conditions, specifically, 5 mL organic solvent mixture of 2-methyl-2-butanol/DMSO (20% v/v), 2 mmol fructose with a lauric acid to fructose molar ratio of 2:1, 0.3 g whole-cell biocatalyst (1,264 U/g dry cell) with an initial water activity of 0.11, 1.2 g 4Å molecular sieve, reaction temperature of 55oC and 200 rpm stirring speed, the fructose mono laurate ester yield was 78% (w/w). The CALBdisplaying P. pastoris whole-cell biocatalyst exhibited good operational stability, with an evident increase, rather than decrease, in relative activity after the continuous recover and reuse cycle. The relative activity of the biocatalyst remained 50% higher than that of the first batch, even following reuse for 15 batches. Our results collectively indicate that the CALB-displaying P. pastoris whole-cell biocatalyst may be potentially utilized in lieu of free or immobilized enzyme to effectively produce non-ionic surfactants such as fatty acid sugar esters, offering the significant advantages of cost-effectiveness, good operational stability and mild reaction conditions.

      • KCI등재

        Tissue-Specific CRISPR/Cas9 System of Cotton Pollen with GhPLIMP2b and GhMYB24 Promoters

        Lei Jianfeng,Dai Peihong,Li Jiyang,Yang Mi,Li Xiuqin,Zhang Wanqi,Zhou Guantong,WangzhenGuo,Liu Xiaodong 한국식물학회 2021 Journal of Plant Biology Vol.64 No.1

        CRISPR/Cas9 technology is a powerful tool for improving crop genetic traits. However, CRISPR/Cas9 system for efficient expression of cotton germ cells has still not been established. In this study, we developed a tissue-specific vectors to drive Cas9 expression with GhPLIMP2b and GhMYB24 promoters and established the effective method to transform cotton pollen by Agrobacterium vacuum infiltration. GhPLIMP2b and GhMYB24 promoters of cotton pollen were cloned into Cas9 expression vectors. The sgRNAs targetting to CLA1, ERA1 and GGB (drought-resistant negative regulation genes) were designed and constructed into GhPLIMP2b and GhMYB24 promoter vectors. Cotton pollens were tranformed by Agrobacterium vacuum infiltration with GhPLIMP2b and GhMYB24 promoter vectors. The results of clone sequencing shown that mutation types of the sequence were mainly base substitutions wth the frequency from 3.29 to 6.45%. Eleven potential off-target sites were chosen and two sites were observed. Our results indicated that GhPLIM2bP::Cas9 and GhMYB24P::Cas9 editing vectors achieved targeted edition of endogenous genes in cotton pollen, but there were a few off-target effects. This study provides an effective gene-editing system for the rapid acquisition of cotton mutants using pollen as a transgenic receptor.

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