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RISS 인기검색어
- 검색키워드
- 저자 : Daichang Yang (검색결과 2 건)
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A Calmodulin-Binding Protein from Rice is Essential to Pollen Development
Qiusheng Zhang,Zhong Li,Jing Yang,Shaoqin Li,Daichang Yang,Yingguo Zhu 한국식물학회 2012 Journal of Plant Biology Vol.55 No.1
A pollen-expressed gene from rice, OsPCBP, that was previously cloned and identified encodes a novel calmodulin-binding protein. Here, we investigated its functioning in pollen development. Overexpression of OsPCBP in transgenic rice plants did not lead to phenotypic changes but did delay anther formation by about 1 week. Cytological observations at different stages revealed that microspores from the OsPCBP double-stranded RNA interference plants developed normally until the binucleate stage, but about half were later aborted. Meanwhile, the anther microstructure of those plants was unaffected. Further examination of OsPCBP expression in transgenic lines via RT-PCR showed that a significant reduction in transcripts was correlated with this abortion phenotype. Our experimental results provide convincing evidence that OsPCBP protein plays an important role during the late stage of pollen development.
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Analysis of ESTs from a Normalized cDNA Library of the Rhizome Tip of Oryza longistaminata
Ting Zhang,Lijuan Li,Fengyi Hu,Xiuqin Zhao,Binying Fu,Daichang Yang 한국식물학회 2012 Journal of Plant Biology Vol.55 No.1
Oryza longistaminata, a perennial wild rice species with an AA genome, is characterized by the presence of rhizomatous stems. The rhizomatous trait in rice was previously shown to be quantitatively controlled by many genes, but the molecular mechanism related to rhizome development is still unknown. In the present study, expressed sequence tags (ESTs) generated from rhizome tips of O. longistaminata were collected and analyzed. A total of 10,283 complimentary deoxyribunucleic acid clones were randomly sequenced, which generated 10,136 raw sequences,and finally, 4,419 unisequences with diverse functional categories were generated. These unisequences were mapped onto the Oryza sativa genome, which revealed that 4,285(96.97%) and 4,151 (93.94%) of the unisequences were alignable to the japonica and indica genomic sequences,respectively, with >80% sequence identity. Additionally, 41unisequences showed four typical types of alternative splicing patterns. More than 600 simple sequence repeats were identified in these unisequences. A subset of unisequences were physically colocalized onto rhizome-related quantitative trait locus intervals in rice and sorghum, and one gene,OLRR1, was further confirmed to be enriched in the rhizome tip and young leaf by real-time polymerase chain reaction and in situ hybridization. Unisequences reported in this study provide valuable data for molecular dissection of the rhizomatous growth habit in O. longistaminata.
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