http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Xiuming Zhang (검색결과 4 건)
- 무료
- 기관 내 무료
- 유료
-
Wene Zhang,Zhiguo Tian,Xuejun Pan,Xiuming Zhao,Fei Wang 한국원예학회 2013 Horticulture, Environment, and Biotechnology Vol.54 No.1
The physiological responses of three edible canna cultivars (Canna edulis ker. cv. ‘PLRF’, ‘Xingyu-1’, and ‘Xingyu-2’) to continuous drought stress for 35 days were investigated by characterizing the water saturation deficit (WSD), relative electrical conductivity (REC), superoxidative radical content (SRC), ascorbic acid (AsA) content,glutathione (GSH) content and protein content. It was observed that WSD, REC, SRC progressively increased in the upper leaves of three cultivars under both control and drought treatments. The content changes of AsA, GSH and water soluble protein were lower in ‘Xingyu-2’ than in ‘Xingyu-1’ and ‘PLRF’, in upper leaves than in lower leaves. Compared with control, drought stress aggravated these physiological changes in all three cultivars. The correlation analysis showed that there were significant correlations between indexes except for protein content, which significantly correlated only with SRC. These indicated that drought stress directly led to water loss, and then the REC increased, while the GSH and AsA played major roles in removing the SRC. These results revealed that ‘Xingyu-2’ was more tolerant to drought stress than ‘PLRF’ and ‘Xingyu-1’,and the lower leaves were more sensitive than the upper leaves. The present study not only provides new insights into mechanisms of acclimation and tolerance to drought stress in edible canna but also provides clues for improving drought tolerance of edible canna through breeding or genetic engineering.
-
Chen Qin,Yu Yilin,Zhang Xiang,Zhao Ren,Zhang Jinyu,Liu Diqiu,Cui Xiuming,Ge Feng 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.5
The ERF-type transcription factors (TFs) play vital roles in plant secondary metabolism. ERF TFs simultaneously regulate the expression levels of key enzyme genes involved in the biosynthesis of secondary metabolites due to its “multi-point control” function. In this study, one gene of ERF TFs from Panax japonicus (PjERF1) was cloned. The open reading frame of PjERF1 was 801 bp and encoded 266 amino acids. Phylogenetic analysis showed that PjERF1 belonged to ERF subfamily with a typical conserved domain. Subcellular localization found that PjERF1 protein might be located in eukaryotic cell nucleus. Yeast one-hybrid assay demonstrated that PjERF1 could bind to the promoters of PjβAS, PjCAS, and PjSE specifically and regulate the expression levels of such key enzyme genes involved in the triterpene saponins biosynthesis. Therefore, in the PjERF1 overexpression cell lines, the expression levels of some key enzyme genes involved in the triterpenoid saponins biosynthesis were significantly increased compared with those in non-transgenic cell line. As a result of it, the biosynthesis of chikusetsusaponin IV and IVa, and other ginsenosides (Rd, Rb1, Re, and R0) were also promoted in the PjERF1 over- expression cell lines. This study indicated that PjERF1 could regulate the biosynthesis of saponins in P. japonicus through controlling the expression levels of key enzyme genes related to the biosynthesis of triterpenoid saponins.
-
Zhiguo Tian,Fei Wang,Wene Zhang,Changming Liu,Xiuming Zhao 한국원예학회 2012 Horticulture, Environment, and Biotechnology Vol.53 No.3
In this study, malondialdehyde (MDA), relative conductivity (RC), superoxide anion (O2・-), hydrogen peroxide (H2O2), superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX) as well as ascorbic acid (AsA), glutation (GSH) and carotenoid (Car) were analyzed in plants under drought condition to investigate the enzymatic and non-enzymatic antioxidant defense mechanisms of leaves and petals, respectively. Two different drought resistance marigold cultivars (Tagetes erecta L. cv. Chokdee and Tagetes erecta L. cv. Discovery)treated with 6-day drought stress were used at early flowering stage. Results indicated that drought treatment increased MDA, RC, O2・- and H2O2 contents in the two cultivars, especially in drought-sensitive cultivar ‘Discovery’. In contrast,‘Chokdee’ had higher level antioxidative enzyme activities and more non-enzymatic antioxidants than those in ‘Discovery’. SOD, POD, CAT, APX activities and non-enzymatic antioxidants (GSH and AsA) in the leaves and petals were increased at the beginning treatment, and decreased later. The activity of CAT in leaves and petals, APX in petals and AsA in petals on day 6 after treatment were lower than those in control, while Car in the two cultivars decreased consistently during drought stress treatment. In addition, all the antioxidant enzyme activities in the leaves were higher than those in petals, but AsA and GSH were accumulated at lower levels in leaves than those in petals of the both cultivars. Furthermore, significant linear relationships were found between antioxidative enzymes and reactive oxygen species (ROS), as well as in non-enzymatic antioxidants and ROS. In conclusion, drought tolerance of ‘Chokdee’ was correlated with eliminating the O2・- and H2O2 and maintaining lower lipid peroxidation as well as higher membrane stability by increasing activities of antioxidative enzymes and the amount of non-enzymatic antioxidants. Furthermore, different drought response mechanisms were involved in leaves and petals of marigold under drought stress.
-
Xuehan Wang,Mingxuan Li,Liwei Liu,Rui Mou,Xiuming Zhang,Yanling Bai,Haijin Xu,Mingqiang Qiao 한국미생물학회 2012 The journal of microbiology Vol.50 No.6
A Pseudomonas aeruginosa mutant strain M122 was isolated from a Mu transposon insertion mutant library. In our prophase research, we have found that PA0058, a novel gene encodes a 234-residue conserved protein, was disrupted in the M122 mutant. In this study, the bacteriostatic experiment in vitro indicates that M122 has abnormally high aminoglycoside resistance. We expressed PA0058 in E. coli and found that PA0058 oxidizes and reduces disulfide. This biochemical characterization suggests that PA0058 is a novel disulfide oxidoreductase. Hence, the protein was designated as DsbM. Microarray analysis of the M122 mutant showed its unusual phenotype might be related to the bacterial antioxidant defense system mediated by the oxyR regulon. Meanwhile, we detected –SH content in the periplasm of M122 and wild strain and found a lower –SH/S–S ratio in M122. Therefore, we consider that the loss of dsbM function decreased the –SH/S–S ratio, which then prolongs the OxyR-regulated response, thereby conferring high aminoglycoside resistance to the M122 mutant strain. Our findings have important implications for understanding the mechanisms underlying aminoglycoside resistance in P. aeruginosa.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
