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        Comparison of Fecal Microbiota of Mongolian and Thoroughbred Horses by High-throughput Sequencing of the V4 Region of the 16S rRNA Gene

        Zhao, Yiping,Li, Bei,Bai, Dongyi,Huang, Jinlong,Shiraigo, Wunierfu,Yang, Lihua,Zhao, Qinan,Ren, Xiujuan,Wu, Jing,Bao, Wuyundalai,Dugarjaviin, Manglai Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.9

        The hindgut of horses is an anaerobic fermentative chamber for a complex and dynamic microbial population, which plays a critical role in health and energy requirements. Research on the gut microbiota of Mongolian horses has not been reported until now as far as we know. Mongolian horse is a major local breed in China. We performed high-throughput sequencing of the 16S rRNA genes V4 hypervariable regions from gut fecal material to characterize the gut microbiota of Mongolian horses and compare them to the microbiota in Thoroughbred horses. Fourteen Mongolian and 19 Thoroughbred horses were used in the study. A total of 593,678 sequence reads were obtained from 33 samples analyzed, which were found to belong to 16 phyla and 75 genera. The bacterial community compositions were similar for the two breeds. Firmicutes (56% in Mongolian horses and 53% in Thoroughbred horses) and Bacteroidetes (33% and 32% respectively) were the most abundant and predominant phyla followed by Spirochaete, Verrucomicrobia, Proteobacteria, and Fibrobacteres. Of these 16 phyla, five (Synergistetes, Planctomycetes, Proteobacteria, TM7, and Chloroflexi) were significantly different (p<0.05) between the two breeds. At the genus level, Treponema was the most abundant genus (43% in Mongolian horses vs 29% in Thoroughbred horses), followed by Ruminococcus, Roseburia, Pseudobutyrivibrio, and Anaeroplasma, which were detected in higher distribution proportion in Mongolian horses than in Thoroughbred horses. In contrast, Oscillibacter, Fibrobacter, Methanocorpusculum, and Succinivibrio levels were lower in Mongolian horses. Among 75 genera, 30 genera were significantly different (p<0.05) between the two breeds. We found that the environment was one of very important factors that influenced horse gut microbiota. These findings provide novel information about the gut microbiota of Mongolian horses and a foundation for future investigations of gut bacterial factors that may influence the development and progression of gastrointestinal disease in horses.

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        Exergy analysis of two-stage steam-water jet injector

        Qin Cai,Mingwei Tong,Xiujuan Bai 한국화학공학회 2012 Korean Journal of Chemical Engineering Vol.29 No.4

        Exergy analysis is used as a tool to evaluate exergy losses in the steam-water jet injector so as to improve its overall performance. What this article addresses here is mainly about a parametric study on the injector under various operating conditions, such as different inlet water temperature, inlet steam pressure, pressure ratio, entrainment ratio and flowrate ratio. In addition, the irreversible losses in the component parts of the two-stage injector were analyzed in detail. The results show that the operating parameters have great effects on exergy efficiency of the injector. The average exergy efficiency of the two-stage injector is 21% more than that of the single-stage one. Moreover, calculations based on experimental data indicate that the highest exergy losses due to irreversibility occur in the first-stage mixing chamber. In light of this comparison, the exergy losses occurring in the system are proportional to the exergy efficiency obtained by applying the system.

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        Comparative analysis of physiological traits of three Actinidia valvata Dunn genotypes during waterlogging and post-waterlogging recovery

        Zhi Li,Yunpeng Zhong,Danfeng Bai,Miaomiao Lin,Xiujuan Qi,Jinbao Fang 한국원예학회 2020 Horticulture, Environment, and Biotechnology Vol.61 No.5

        Kiwifruit ( Actinidia sp.) vines are poorly adapted to waterlogging stress. Actinidia valvata is more tolerant against waterloggingstress than Actinidia deliciosa , and the latter contains some common rootstocks that are frequently used in kiwifruit cultivation. Little is known about the responses of A. valvata genotypes against waterlogging stress and during post-waterloggingrecovery. Here, we compared physiological traits of three A. valvata genotypes (KR1, KR3, and KR5) during waterloggingstress and recovery. Kiwifruit vines displayed water loss, a decline in the net photosynthetic rate, and inhibited shoot elongationduring waterlogging. These three genotypes could endure long-term waterlogging owing to their unique root systemconfi gurations as well as by sustaining carbohydrate reserves in the roots. Feeder roots of KR1 vines were damaged earlierand lost water more quickly than the other genotypes. Under the same stress, KR3 formed adventitious roots more rapidly,while KR5 had an improved ability to control water loss in above-ground tissues. After reoxygenation, growth of vineswas partially recovered due to water loss control, photosynthetic recovery, and carbohydrate replenishment. KR3 and KR5recovered their growth earlier and replenished more carbohydrates than KR1 after re-aeration. During waterlogging, both therelative water content and carbohydrate levels of vines can limit the recovery effi ciency after re-aeration. Our results revealedmutual and distinct responses of diff erent A. valvata genotypes during waterlogging stress and recovery and provided moreinsight into the physiological basis of their adaptation to waterlogging stress.

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