Two New Species of Placolecis (Lichenized Ascomycota) from China

( An Cheng Yin ),( Xin Yu Wang ),( Dong Liu ),( Yan Yun Zhang ),( Mei Xia Yang ),( Li Juan Li ),( Li Song Wang ) 한국균학회 2019 Mycobiology Vol.47 No.4

Two new species of the lichen genus Placolecis are discovered in China, namely P. kunmingensis An. C. Yin & Li S. Wang and P. sublaevis An. C. Yin & Li S. Wang. The new combination P. loekoesiana (S.Y. Kondr., Farkas, J.J. Woo & Hur) An. C. Yin is proposed. Placolecis kunmingensis is characterized by having simple, spherical or ellipsoid, hyaline spores, and pear-shaped pycnidia; while P. sublaevis can be distinguished by its thallus forming larger aggregations with slightly flattened lobes at the thallus margin, and urn-shaped pycnidia. Descriptions, a phylogenetic tree and a key are provided for all the known Placolecis species in China.

Extremal problems on the Cartan-Hartogs domains

An Wang,Xin Zhao,Zhiyin Liu 대한수학회 2007 대한수학회지 Vol.44 No.6

Preparation of activated carbon spheres and their electrochemical properties as supercapacitor electrode

An Xin,Xing Guannan,Wang Jing,Tian Yanhong,Liu Yunfang,Wan Qiong 한국탄소학회 2021 Carbon Letters Vol.31 No.4

The carbon spheres (CSs) synthesized by an ultrasonic-spray pyrolysis method were activated for supercapacitor electrode. There are plenty of cracks on the surface of the activated carbon spheres (ACSs), which expend with increasing the activation temperature and activator dosage. The specifc capacitance of ACSs increases with the activation temperature and activator dosage and reach to maximal value at certain conditions. Importantly, the ACS sample activated at relatively low activation temperature (600 °C) and 7 of mass ratio of KOH to CSs has the highest specifc capacitance (about 209 F g−1 at 50 mA g−1 of current density) and indicates the excellent cycling stability after 1000 consecutive charge–discharge cycles. Furthermore, the graphene sheets could be found in the samples that were activated at 1000 °C. And the electrode prepared by the sample has the very low series resistance because of the excellent conductivity of the formed graphene sheets.

Differential Protein Expression in EC304 Gastric Cancer Cells Induced by Alphastatin

Wang, Xin-Xin,Sun, Rong-Ju,Wu, Meng,Li, Tao,Zhang, Yong,Chen, Lin Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

Objective: To explore the differential protein expression profile in EC304 gastric cancer cells induced by alphastatin. Methods: Cultured EC304 cells in the exponential phase of growth were randomly divided into alphastatin and control groups. Total proteins were extracted and the two dimensional electrophoresis (2-DE) technique was applied to analyze differences in expression with ImageMaster 2D Platinum 5.0 software. Proteins were identified using the MASCOT database and selected differently expressed proteins were characterised by western blotting and immunofluorescence. Results: $1350{\pm}90$ protein spots were detected by the ImageMaster software in the 2-DE gel images from the control and alphastatin groups. The match rate was about 72-80% for the spectrum profiles, with 29 significantly different protein spots being identified, 10 upregulated, 16 downregulated, two new and one lost. The MASCOT search scores were 64-666 and the peptide matching numbers were 3-27 with sequence coverage of 8-62%. Twenty-three proteins were checked by mass spectrometry, including decrease in Nm23 and profilin-2 isoform b associated with the regulation of actin multimerisation induced by extracellular signals. Conclusion: The proteome in EC304 cells is dramatically altered by alphastatin, which appears to play an important role in modulating cellular activity and anti-angiogenesis by regulating protein expression and signal transduction pathways through Nm23 and profilin-2 isoform b, providing new research directions for anti-angiogenic therapy of gastric cancer.

CEA, AFP, CA125, CA153 and CA199 in Malignant Pleural Effusions Predict the Cause

Wang, Xin-Feng,Wu, Yan-Hua,Wang, Mao-Shui,Wang, Yun-Shan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.1

Determination of the cause of malignant pleural effusions is important for treatment and management, especially in cases of unknown primaries. There are limited biomarkers available for prediction of the cause of malignant pleural effusion in clinical practice. Hence, we evaluated pleural levels of five tumor biomarkers (CEA, AFP, CA125, CA153 and CA199) in predicting the cause of malignant pleural effusion in a retrospective study. Kruskal-Wallis or Mann-Whitney U tests were carried out to compare levels of tumor markers in pleural effusion among different forms of neoplasia - lung squamous cell carcinoma, adenocarcinoma, or small cell carcinoma, mesothelioma, breast cancer, lymphoma/leukemia and miscellaneous. Receiver operator characteristic analysis was performed to evaluate sensitivity and specificity of biomarkers. The Kruskal-Wallis test showed significant differences in levels of pleural effusion CEA (P<0.01), AFP (P<0.01), CA153 (P<0.01) and CA199 (P<0.01), but not CA125 (P>0.05), among the seven groups. Receiver operator characteristic analysis showed that, compared with other four tumor markers, CA153 was the best biomarker in diagnosing malignant pleural effusions of lung adenocarcinoma (area under curve (AUC): 0.838 (95%confidence interval: 0.787, 0.888); cut-off value: 10.2U/ml; sensitivity: 73.2% (64.4-80.8)%, specificity: 85.2% (77.8-90.8)%), lung squamous cell carcinoma (AUC: 0.716 (0.652, 0.780); cut-off value: 14.2U/ml; sensitivity: 57.6% (50.7-64.3)%, specificity: 91.2% (76.3-98.0)%), and small-cell lung cancer (AUC: 0.812 (0.740, 0.884); cut-off value: 9.7U/ml; sensitivity: 61.5% (55.0-67.8)%, specificity: 94.1% (71.2-99.0)%); CEA was the best biomarker in diagnosing MPEs of mesothelioma (AUC: 0.726 (0.593, 0.858); cut-off value: 1.43ng/ml; sensitivity: 83.7% (78.3-88.2)%, specificity: 61.1% (35.8-82.6)%) and lymphoma/leukemia (AUC: 0.923 (0.872, 0.974); cut-off value: 1.71ng/ml; sensitivity: 82.8% (77.4-87.3)%, specificity: 92.3% (63.9-98.7)%). Thus CA153 and CEA appear to be good biomarkers in diagnosing different causes of malignant pleural effusion. Our findings implied that the two tumor markers may improve the diagnosis and treatment for effusions of unknown primaries.

Susceptibility Loci Associations with Prostate Cancer Risk in Northern Chinese Men

Wang, Na-Na,Xu, Yong,Yang, Kuo,Wei, Dong,Zhang, Yao-Guang,Liu, Ming,Shi, Xiao-Hong,Liang, Si-Ying,Sun, Liang,Zhu, Xiao-Quan,Yang, Yi-Ge,Tang, Lei,Zhao, Cheng-Xiao,Wang, Xin,Chen, Xin,Hui, Juan,Zhang, Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5

Background: KLK3 gene products, like human prostate-specific antigen (PSA), are important biomarkers in the clinical diagnosis of prostate cancer (PCa). G protein-coupled receptor RFX6, C2orf43 and FOXP4 signaling plays important roles in the development of PCa. However, associations of these genes with PCa in northern Chinese men remain to be detailed. This study aimed to investigate their impact on occurrence and level of malignancy. Methods: All subjects were from Beijing and Tianjin, including 266 cases with prostate cancer and 288 normal individuals as controls. We evaluated associations between clinical covariates (age at diagnosis, prostate specific antigen, Gleason score, tumor stage and aggressive) and 6 candidate PCa risk loci, genotyped by PCR- high resolution melting curve and sequencing methods. Results: Case-control analysis of allelic frequency of PCa associated with PCa showed that one of the 6 candidate risk loci, rs339331 in the RFX6 gene, was associated with reduced risk of prostate cancer (odds ratio (OR) = 0.73, 95% confidence interval (CI) =0.57-0.94, P = 0.013) in northern Chinese men. In addition, subjects with CX (CC+TC) genotypes had a decreased risk for prostrate cancer compared to those carrying the TT homozygote (OR =0.64, 95% CI = 0.45- 0.90, P = 0.008). The TT genotype of 13q22 (rs9600079, T) was associated with tumor stage (P=0.044, OR=2.34, 95% CI=0.94-5.87). Other SNPs were not significantly associated with clinical covariates in prostate cancer (P > 0.05). Conclusions. rs339331 in the RFX6 gene may be associated with prostate cancer as a susceptibility locus in northern Chinese men.

The Biocompatibility of Multi-Source Stem Cells and Gelatin-Carboxymethyl Chitosan-Sodium Alginate Hybrid Biomaterials

Wang Xinzhe,Li Siqi,Yu Honglian,Lv Jianzhi,Fan Minglun,Wang Ximing,Wang Xin,Liang Yanting,Mao Lingna,Zhao Zhankui 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.3

BACKGROUND: Nowadays, biological tissue engineering is a growing field of research. Biocompatibility is a key indicator for measuring tissue engineering biomaterials, which is of great significance for the replacement and repair of damaged tissues. METHODS: In this study, using gelatin, carboxymethyl chitosan, and sodium alginate, a tissue engineering material scaffold that can carry cells was successfully prepared. The material was characterized by Fourier transforms infrared spectroscopy. In addition, the prepared scaffolds have physicochemical properties, such as swelling ratio, biodegradability. we observed the biocompatibility of the hydrogel to different adult stem cells (BMSCs and ADSCs) in vivo and in vitro. Adult stem cells were planted on gelatin-carboxymethyl chitosan-sodium alginate (Gel/SA/CMCS) hydrogels for 7 days in vitro, and the survival of stem cells in vitro was observed by live/died staining. Gel/SA/CMCS hydrogels loaded with stem cells were subcutaneously transplanted into nude mice for 14 days of in vivo culture observation. The survival of adult stem cells was observed by staining for stem cell surface markers (CD29, CD90) and Ki67. RESULTS: The scaffolds had a microporous structure with an appropriate pore size (about 80 lm). Live/died staining showed that adult stem cells could stably survive in Gel/SA/CMCS hydrogels for at least 7 days. After 14 days of culture in nude mice, Ki67 staining showed that the stem cells supported by Gel/SA/CMCS hydrogel still had high proliferation activity. CONCLUSION: Gel/SA/CMCSs hydrogel has a stable interpenetrating porous structure, suitable swelling performance and degradation rate, can promote and support the survival of adult stem cells in vivo and in vitro, and has good biocompatibility. Therefore, Gel/SA/CMCS hydrogel is a strong candidate for biological tissue engineering materials.

Effect of passivator DHJ-C on the growth and cadmium accumulation of Brassica napus in Cd-contaminated soil

Wang Xin,Liu Qian,Wan Yunbao,Cao Chenxi,Bai Jiuyuan,Wang Rui,Zhao Yun 한국응용생명화학회 2021 Applied Biological Chemistry (Appl Biol Chem) Vol.64 No.2

The farmland polluted by cadmium is increasing drastically, which seriously threatened agricultural production and food safety. Nowadays, efficient and convenient way to solve the problem is urgently needed. In this experiment, a particular compound passivator DHJ-C was applied for soil remediation by pot experiment and the effect on both soil and plant was evaluated. The DHJ-C reduced the toxicity of Cd on soil enzyme activity and growth inhibition on Brassica napus. The soil urease and sucrase activity were significantly increased. The dry weight of mature oilseed rape increased by 14.6–36.0% and the yield of seeds increased by 14.1–52% per plant, which suggested that the passivator effectively reduced the detrimental effects on rape. Similarly, the results of physiology and biochemistry also indicated that DHJ-C can distinctly alleviate the inhibitory effect of Cd on plant growth. Such as the MDA content in plant was reduced by 52.1% in 10 mg/kg Cd treatment. Compared with control, Cd accumulation in seedling stage and mature period was significantly reduced as the concentration of Cd in aboveground part even decreased by 18.4 and 32.0% respectively. Overall, DHJ-C hold sufficient ability to be applied as an excellent passivator to reduce Cd toxicity in contaminated soil and significantly increase the yield of rapeseed.

A Novel Configuration Method of Virtual Machine Fitness

Wang Xin,Wang Ning 보안공학연구지원센터 2016 International Journal of Grid and Distributed Comp Vol.9 No.11

Virtual machine resource allocation problem is closely related to the resource requirement of physical host, and a novel configuration method of virtual machine is proposed. First, the system analyzes the resource requirements of the related virtual machine to the physical host. Secondly, with the help of the similarity between the virtual machine, the fitness is configurated. Secondly, based on the degree of adaptation, the scheduling of the related virtual machine is completed. Two typical methods are selected and compared with the method in this paper. The experimental results confirm the effectiveness of the new method.

A Video Traffic Flow Detection System Based on Machine Vision

Wang, Xin-Xin,Zhao, Xiao-Ming,Shen, Yu Korea Information Processing Society 2019 Journal of information processing systems Vol.15 No.5

This study proposes a novel video traffic flow detection method based on machine vision technology. The three-frame difference method, which is one kind of a motion evaluation method, is used to establish initial background image, and then a statistical scoring strategy is chosen to update background image in real time. Finally, the background difference method is used for detecting the moving objects. Meanwhile, a simple but effective shadow elimination method is introduced to improve the accuracy of the detection for moving objects. Furthermore, the study also proposes a vehicle matching and tracking strategy by combining characteristics, such as vehicle's location information, color information and fractal dimension information. Experimental results show that this detection method could quickly and effectively detect various traffic flow parameters, laying a solid foundation for enhancing the degree of automation for traffic management.