Influence of Peanut Cultivars and Environmental Conditions on the Diversity and Community Composition of Pod Rot Soil Fungi in China

Wang, Mian,Chen, Mingna,Yang, Zhen,Chen, Na,Chi, Xiaoyuan,Pan, Lijuan,Wang, Tong,Yu, Shanlin,Guo, Xingqi The Korean Society of Mycology 2017 Mycobiology Vol.45 No.4

Peanut yield and quality are seriously affected by pod rot pathogens worldwide, especially in China in recent years. The goals of this study are to analyze the structure of fungal communities of peanut pod rot in soil in three peanut cultivars and the correlation of pod rot with environmental variables using 454 pyrosequencing. A total of 46,723 internal transcribed spacer high-quality sequences were obtained and grouped into 1,706 operational taxonomic units at the 97% similarity cut-off level. The coverage, rank abundance, and the Chao 1 and Shannon diversity indices of the operational taxonomic units were analyzed. Members of the phylum Ascomycota were dominant, such as Fusarium, Chaetomium, Alternaria, and Sordariomycetes, followed by Basidiomycota. The results of the heatmap and redundancy analysis revealed significant variation in the composition of the fungal community among the three cultivar samples. The environmental conditions in different peanut cultivars may also influence on the structure of the fungal community. The results of this study suggest that the causal agent of peanut pod rot may be more complex, and cultivars and environmental conditions are both important contributors to the community structure of peanut pod rot fungi.

A Novel MAP Kinase Gene in Cotton (Gossypium hirsutum L.), GhMAPK, is Involved in Response to Diverse Environmental Stresses

Wang, Meimei,Zhang, Ying,Wang, Jian,Wu, Xiaoliang,Guo, Xingqi Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.3

The mitogen-activated protein kinase (MAPK) cascade is one of the major and evolutionally conserved signaling pathways and plays pivotal role in the regulation of stress and developmental signals in plants. Here, a novel gene, termed Gossypium hirsutum MAPK (GhMAPK), was isolated from cotton. The full-length cDNA of GhMAPK encodes for a 372 amino acid protein that contains all 11 of the MAPK conserved subdomains and the phosphorylationactivation motif, TEY. Amino acid sequence alignment revealed that GhMAPK shared high identity with group-C MAPK in plants and showed 83~89% similarities with MAPKs from Arabidopsis, apricot, pea, petunia, and tobacco. Southern blot analysis indicated that the GhMAPK belonged to a multygene family in cotton. Two introns were found within the region of genomic sequence. Northern blot analysis revealed that the transcripts of GhMAPK accumulated markedly when the cotton seedlings were subjected to various abiotic stimuli such as wounding, cold (4$^{\circ}C$), or salinity stress; Furthermore, GhMAPK was upregulated by the exogenous signaling molecules, such as salicylic acid (SA) and hydrogen peroxide ($H_2O_2C$), as well as pathogen attacks. These results indicate that the GhMAPK, which has a high degree of identity with group-C plant MAPKs, may also play an important role in response to environmental stresses.

Influence of Peanut Cultivars and Environmental Conditions on the Diversity and Community Composition of Pod Rot Soil Fungi in China

( Mian Wang ),( Mingna Chen ),( Zhen Yang ),( Na Chen ),( Xiaoyuan Chi ),( Lijuan Pan ),( Tong Wang ),( Shanlin Yu ),( Xingqi Guo ) 한국균학회 2017 Mycobiology Vol.45 No.4

Peanut yield and quality are seriously affected by pod rot pathogens worldwide, especially in China in recent years. The goals of this study are to analyze the structure of fungal communities of peanut pod rot in soil in three peanut cultivars and the correlation of pod rot with environmental variables using 454 pyrosequencing. A total of 46,723 internal transcribed spacer high-quality sequences were obtained and grouped into 1,706 operational taxonomic units at the 97% similarity cut-off level. The coverage, rank abundance, and the Chao 1 and Shannon diversity indices of the operational taxonomic units were analyzed. Members of the phylum Ascomycota were dominant, such as Fusarium, Chaetomium, Alternaria, and Sordariomycetes, followed by Basidiomycota. The results of the heatmap and redundancy analysis revealed significant variation in the composition of the fungal community among the three cultivar samples. The environmental conditions in different peanut cultivars may also influence on the structure of the fungal community. The results of this study suggest that the causal agent of peanut pod rot may be more complex, and cultivars and environmental conditions are both important contributors to the community structure of peanut pod rot fungi.

Effect of asymmetrical tip clearances on energy performance and cavitation characteristics of NACA0009 hydrofoil in tidal energy

Like Wang,Xingqi Luo,Jinling Lu,Jianjun Feng,Guojun Zhu,Wei Wang,Kai Wang 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.9

Tidal energy is an important renewable energy that uses the potential energy created by the rising and falling of ocean tides to generate electricity. The tip leakage vortex (TLV) and cavitation caused by the tip leakage flow have adverse effects on the energy conversion efficiency and stable operation of the tubular turbine. The selection of the tip clearance shape is critical. Therefore, the effects of four different combinations of asymmetrical tip clearance shapes on the energy and cavitation characteristics of hydrofoils are studied in this paper. The results show that the TLV remains unchanged when tipwall and endwall are sinusoidal and cosine curve shaped, respectively. When tip clearance is the combination of a flat tipwall and a sinusoidal endwall, the lift-drag ratio decreases by 10.72 %. The high shear stress region of tipwall near leading edge decreases. The flow resistance of the leakage flow decreases, and the leakage flow becomes more serious. The cavitation volume is 2.5 times that under the original flat tip clearance. When tip clearance is the combination of a flat tipwall and a cosine endwall, and the lift-drag ratio increases by 9.67 %. The shear stress increases, the leakage flow decreases. The swirling strength around the center of the vortex core is weakened. The cavitation volume is 3.81, with a decrease of nearly 30 %. A proper asymmetrical tip clearance can effectively improve the energy performance and cavitation characteristics of hydrofoils. This study provides theoretical support for the design and optimization of hydraulic machine with tip clearance.

Molecular Characterization of a Transient Expression Gene Encoding for 1-Aminocyclopropane-1-carboxylate Synthase in Cotton (Gossypium hirsutum L.)

Wang, Xia,Zhang, Ying,Zhang, Jiedao,Cheng, Cheng,Guo, Xingqi Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.5

Ethylene performs an important function in plant growth and development. 1-aminocyclopropane-1-carboxylate (ACC) synthase (ACS), the key enzyme involved in ethylene biosynthesis, has been the focus of most ethylene studies. Here, a cotton ACS gene referred to as Gossypium hirsutum ACS1 (GhACS1), was isolated. The full-length cDNA of GhACS1 encodes for a 476-amino acid protein which harbors seven conserved regions, 11 invariant amino acid residues, and the PLP binding active site, all of which characterize ACC synthases. Alignment analysis showed that GhACS1 shared a high degree of identity with other known ACC synthases from different species. Two introns were detected in the genomic DNA sequence, and the results of Southern blot analysis suggested that there might be a multi-gene family encoding for ACC synthase in cotton. From the phylogenetic tree constructed with 24 different kinds of ACC synthases, we determined that GhACS1 falls into group II, and was closely associated with the wound-inducible ACS of citrus. The analysis of the 5' flanking region of GhACS1 revealed a group of putative cis-acting elements. The results of expression analysis showed that GhACS1 displayed its transient expression nature after wounding, abscisic acid (ABA), and $CuCl_2$ treatments. These results indicate that GhACS1, which was transiently expressed in response to certain stimuli, may be involved in the production of ethylene for the transmission of stress signals.

A Robust Approach for Overlay Text Localization and Extraction in Complex Video Scene

Jingfan Tang,Zhitao Li,Xingqi Wang,Ming Jiang,Ziyang Li 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.10

Overlay text in video carries important semantic clues for video information retrieval and summarization. In this paper, we propose a robust method that is able to accurately locate text lines and extract text even in complex video scene. In the text localization stage, this paper adopts the method based on corner point. First, corner detection is used to extract corners as text features from video frames. Then multi-layer filtering mechanism (MLFM) is used to locate the text lines, which consists of corners clustering, corners horizontal projection, background filtering and heuristic rules. This MLFM can effectively remove the isolated corners, locate the text lines accurately and remove the background or pseudo text lines automatically. In the text extraction stage, this paper proposed a twice binarization method that combines with polarity judgment on image. The polarity judgment was used as a guide to adjust the first binarization threshold when we perform the first binarization. After the first binarization, a main proportion of the image has been processed, and the rest will be processed by the second binarization. Experimental results show that this approach can fast and robustly locate text lines and extract text in video even under complex background.