Research on customer complaints in the background of industry 4.0

Xiaomin SUN 한국인공지능학회 2020 인공지능연구 (KJAI) Vol.8 No.2

Purpose: Today, we often hear complaints from customers: poor quality, poor service, expensive prices, etc. Customer complaints are an indication that the company's products and services do not meet customer requirements, which in turn causes customer complaints. An important content of corporate marketing practice is how to use the opportunity of handling customer complaints to win the trust of customers and gain a competitive advantage. According to the concept of marketing, the way for an enterprise to obtain profits is to continuously meet the needs of customers. However, with increasingly fierce market competition and the overall formation of a buyer's market, providing highquality products and high-efficiency and high-level services have become the eternal theme of enterprises. Therefore, meeting the actual needs of customers and effectively handling customer complaints are issues that we must take seriously. Research design, data, and methodology: This article mainly analyzes the causes of customer complaints, proposes relevant solutions for different types of complaints, builds a customer complaint management system, improves the efficiency and ability of handling complaints, and provides more references and basis for enterprises to solve customer complaints. Conclusions: To further improve the quality of enterprise products and service standards, to help enterprises increase customer loyalty and satisfaction, and to enable enterprises to gain advantages in the increasingly competitive global market.

The role of CTNNB1 and LEF1 in feather follicles development of Anser cygnoides and Anser anser

Yue Sun,Yuxuan Zhou,Petunia Msuthwana,Jing Liu,Chang Liu,Cornelius Tlotliso Sello,Yupu Song,Ziqiang Feng,Shengyi Li,Wei Yang,Yunpeng Xu,Xiaomin Yan,Chuanghang Li,Yujian Sui,Jingtao Hu,Yongfeng Sun 한국유전학회 2020 Genes & Genomics Vol.42 No.7

Background Wingless-types/beta-catenin (Wnt/β-catenin) signaling pathway is one of the most extensively studied transcriptional cascades involved in various types of organogenesis including embryonic and postnatal development. Downy feather quantity is primarily affected by follicular development and gene regulations. Objective This research was aimed to investigate the role of catenin beta-1(CTNNB1) and lymphoid enhancerbinding factor-1 (LEF1) on feather follicles development at different developmental stages. Methods Fluorescence quantitative PCR, Western-blot and immunohistochemical methods were used in Anser cygnoides and Anser anser embryos (E12, E13 E18, and E28) and after birth gosling stages (G18, G48, G88) for gene expression analysis. Results CTNNB1 and LEF1 genes were expressed in Anser cygnoides and Anser anser at different embryonic and after-birth gosling developmental stages and the expression levels were significantly different in different stages (p < 0.05). The mRNA expression of CTNNB1 and LEF1 genes reached the highest level at D88 in Anser cygnoides, while the highest expression levels were at D18 and D88 in Anser anser, and the expression levels of CTNNB1 genes at D88 in all embryonic stages were significantly lower than after-birth stages. CTNNB1 and LEF1 protein expression were the highest at E12 and E28 for Anser cygnoides feather follicles development. While at a similar stage for Anser anser, the expression of CTNNB1 and LEF1 protein was the highest at D48 and D18. Protein expression at embryonic stages was in the epidermis (E) and the hair basal plate (P), the expression site for after-birth stages was in the dermal papilla (DP). Conclusion Our study illustrated that CTNNB1 and LEF1 has an impact on Anser cygnoides and Anser anser feather follicles growth and development.

Ternary Phase Diagram-Facilitated Rapid Screening of Double Perovskites As Electrocatalysts for the Oxygen Evolution Reaction

Sun, Hainan,Hu, Zhiwei,Xu, Xiaomin,He, Juan,Dai, Jie,Lin, Hong-Ji,Chan, Ting-Shan,Chen, Chien-Te,Tjeng, Liu Hao,Zhou, Wei,Shao, Zongping American Chemical Society 2019 Chemistry of materials Vol.31 No.15

<P>The development of cost-effective, non-noble metal electrocatalysts for the oxygen evolution reaction (OER) is of paramount importance for sustainable technologies. Efficient screening strategies for electrocatalysts can greatly increase the commercialization speed of these advanced technologies. Here, ternary phase diagrams with large-scale tuning and designated-scale tuning strategies are applied for the first time to provide a new method for screening perovskite oxide-based electrocatalysts for OERs. Specifically, the family of double perovskites (Sr<SUB>2</SUB>Fe<SUB>1.5-<I>x</I>-<I>y</I></SUB>Co<SUB><I>x</I></SUB>Ni<SUB><I>y</I></SUB>Mo<SUB>0.5</SUB>O<SUB>6−δ</SUB>, 0 ≤ <I>x</I>, <I>y</I> ≤ 1.5) was utilized to understand the role of transition metals in perovskite oxides. Ternary phase diagrams can facilitate a rapid screening process, provide a straightforward relationship between phase structures and catalytic activities, and help to confirm the effects of various combinations of transition metals on the OER activity. The Fe-Co system (Sr<SUB>2</SUB>Fe<SUB>1.5-<I>x</I></SUB>Co<SUB><I>x</I></SUB>Mo<SUB>0.5</SUB>O<SUB>6−δ</SUB>) improves the catalytic activities, as demonstrated by the reduced Tafel slope and enhanced stability, while the Fe-Ni system (Sr<SUB>2</SUB>Fe<SUB>1.5-<I>y</I></SUB>Ni<SUB><I>y</I></SUB>Mo<SUB>0.5</SUB>O<SUB>6−δ</SUB>) improves the surface kinetic properties of the OER, as demonstrated by its reduced overpotential. Significantly, the Co, Ni, and Fe ternary phase systems can serve as the synergistic coactive sites (Sr<SUB>2</SUB>Fe<SUB>1.5-<I>x</I>-<I>y</I></SUB>Co<SUB><I>x</I></SUB>Ni<SUB><I>y</I></SUB>Mo<SUB>0.5</SUB>O<SUB>6−δ</SUB>) to catalyze the OER, resulting in an improved overall OER performance. This systematic study not only demonstrates a new strategy to allow the rapid screening of double perovskite OER catalysts based on large-scale tuning and designated-scale tuning strategies but, more importantly, also provides an insightful understanding of the use of multitransition metal-based double perovskites for catalysis of the OER.</P> [FIG OMISSION]</BR>

Boosting the oxygen evolution reaction activity of a perovskite through introducing multi-element synergy and building an ordered structure

Sun, Hainan,Xu, Xiaomin,Hu, Zhiwei,Tjeng, Liu Hao,Zhao, Jie,Zhang, Qin,Lin, Hong-Ji,Chen, Chien-Te,Chan, Ting-Shan,Zhou, Wei,Shao, Zongping The Royal Society of Chemistry 2019 Journal of Materials Chemistry A Vol.7 No.16

<P>If different active sites in a catalyst have optimal binding to different reaction intermediates and short reaction paths among them, they may work cooperatively to enhance the oxygen evolution reaction (OER) activity. Based on this design principle, in this study, we start with a B-site ordered double perovskite Sr2FeMoO6−δ with poor OER activity as the host material to fulfill the requirement of a short pathway, and then, replace Mo with Ni and Fe with Co to optimize the synergistic interplay of the multi-active sites. Replacing Mo with Ni indeed dramatically enhances the OER activity and structural/operating stability. Further improvement in OER performance is realized by partial substitution of Fe with Co, leading to the development of a material with the nominal composition of Sr2Fe0.8Co0.2Mo0.65Ni0.35O6−δ, which outperforms the noble metal oxide IrO2 and is better than most of the electrocatalysts developed based on a single descriptor, such as Ba0.5Sr0.5Co0.8Fe0.2O3−δ (eg occupancy close to unity), PrBaCo2O5+δ (O 2p-band center relative to the Fermi level), and La0.5Sr0.5CoO3−δ (charge-transfer energy) in many aspects. As a universal method, combined structural and compositional tuning to create a cooperative effect among different active sites for intermediate adsorption and reaction in an ordered structure may provide a new way for the design of superior electrocatalysts for various applications.</P>

Research on Antenna Arraying Combining Technology for Deep Space Measurement & Control

Ke Sun,Xiaomin Hou 보안공학연구지원센터 2016 International Journal of Smart Home Vol.10 No.7

In this dissertation, the key technology of uplink array for deep space TTC is studied. Based on the concept of power combining, the theoretical analysis is made on the uplink array. The spatial power combining can be achieved in a certain power. The uplink antenna array technology is a power combiner. At first this chapter introduces the principle of power combining and leads to the concept of the uplink antenna arraying. Then analyze the theory of the uplink antenna arraying power combining, including the signal strength and antenna pattern. The influence of the number of element, spacing, phase and time delay on power combining is analyzed by simulation. At last the uplink antenna arraying power combining performance is analyzed and verified by simulation.

Construction of g/C3N4-ZnO composites with enhanced visible-light photocatalytic activity for degradation of amoxicillin

Shuhan Sun,Shiling Li,Yibing Hao,Xiao Yang,Xiaomin Dou 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.12

g/C3N4-ZnO composite catalysts were synthesized through surface hybridization of the delocalized conjugated- structure of g/C3N4 with the closely contacted surface of ZnO via a successive and simultaneous calcinationprocedure, and two kinds of photocatalysts, g/C3N4-ZnO1 and g/C3N4-ZnO2, were obtained. Heterojunctions wereformed between the two components, which promote the separation of photogenerated carriers efficiently, and thenenhanced the degradation of 100mg/L of AMX. The degradation rate of g/C3N4-ZnO1 was 1.54, 11.33, and 2.52-foldthat of g/C3N4-ZnO2, g/C3N4, and ZnO, respectively, at a 3.5-h reaction period, with the dosage of 0.3 g/L, and solutionpH at 7.0±0.2. The recycle and reuse ability was excellent and 90.5% of AMX mitigation was achieved in the fifthcycle. For g/C3N4-ZnO1, electrons migrated from the conduction band of g/C3N4 to that of ZnO via the heterojunction. ·OH and h+ were the main active species for AMX degradation, compared to ·O2 dominated for g/C3N4. Twelveintermediate products were identified, and two degradation pathways were inferred for g/C3N4-ZnO1 and g/C3N4-ZnO2, respectively. Finally, transformation products without lactam rings were achieved, which lost most of the antibacterialpotencies, and the ecotoxicity was also dramatically decreased as indicated by the ECOSAR program.

Identification and Genotypic Characterization of Potentially Pathogenic Acanthamoeba Isolated from Tap Water in Wuxi, China

Meixu Wang,Guangxu Sun,Yangkai Sun,Xiaomin You,Xiaoxue Li,Yang Cheng,Yinghua Xuan 대한기생충학열대의학회 2018 The Korean Journal of Parasitology Vol.56 No.6

Establishment and Application of Target Gene Disruption System in Saccharomyces boulardii

Longjiang Wang,Hui Sun,Jie Zhang,Qing Liu,Tiantian Wang,Peipei Chen,Hongmei Li,Yihong Xiao,Fangkun Wang,Xiaomin Zhao 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.1

Saccharomyces boulardii is the best knownprobiotic yeast, widely used as a therapeutic agent for thetreatment or prevention of diarrhea and intestine disorders. In the present work, we established a target gene disruptionsystem for S. boulardii based on the Cre-loxP system usedfor S. cerevisiae and other fungi by screening out selectionmarkers, working out the transformation method, andconstructing essential plasmids for S. boulardii. Theestablished system was successfully applied to the URA3gene disruption and created an ura3 null mutant strain ofS. boulardii. The system can be used for PCR mediatedgene disruption, cloning mediated gene disruption, andreintroduction of the deleted gene back to the mutant. Allthe introduced exogenous DNAs in the gene disruptionprocedures were removed from the final mutant strainexcept the two 34 bp loxP pieces left in deleted gene loci.

Isoforms of wild type proteins often appear as low molecular weight bands on SDS-PAGE.

Zhang, Ju,Lou, Xiaomin,Shen, Haihong,Zellmer, Lucas,Sun, Yuan,Liu, Siqi,Xu, Ningzhi,Liao, D Joshua Wiley 2014 Biotechnology Journal Vol.9 No.8

<P>Immunoblotting, after polyacrylamide gel electrophoresis with sodium dodecyl sulfate (SDS-PAGE), is a technique commonly used to detect specific proteins. SDS-PAGE often results in the visualization of protein band(s) in addition to the one expected based on the theoretical molecular mass (TMM) of the protein of interest. To determine the likelihood of additional band(s) being nonspecific, we used liquid chromatography - mass spectrometry to identify proteins that were extracted from bands with the apparent molecular mass (MM) of 40 and 26 kD, originating from protein extracts derived from non-malignant HEK293 and cancerous MDA-MB231 (MB231) cells separated using SDS-PAGE. In total, approximately 57% and 21% of the MS/MS spectra were annotated as peptides in the two cell samples, respectively. Moreover, approximately 24% and 36.2% of the identified proteins from HEK293 and MB231 cells matched their TMMs. Of the identified proteins, 8% from HEK293 and 26% from MB231 had apparent MMs that were larger than predicted, and 67% from HEK293 and 37% from MB231 exhibited smaller MM values than predicted. These revelations suggest that interpretation of the positive bands of immunoblots should be conducted with caution. This study also shows that protein identification performed by mass spectrometry on bands excised from SDS-PAGE gels could make valuable contributions to the identification of cancer biomarkers, and to cancer-therapy studies.</P>

Use of Ecosystem Flux Data and a Simulation Model to Examine Seasonal Drought Effects on a Subtropical Coniferous Forest

Na Mi,Guirui Yu,Wuefa Wen,Xiaomin Sun,Shusen Wang,Leiming Zhang,Xia Song 한국기상학회 2009 Asia-Pacific Journal of Atmospheric Sciences Vol.45 No.2

The Qianyanzhou (QYZ) forest site is located in the mid-subtropical region under typical subtropical continentalmonsoon climate. Despite its plentiful water and heat resources, changes in their seasonal distributions result in frequent occurrences of seasonal drought, influencing both the structure and function of forest ecosystems. In order to examine the effects of drought stress on energy and water fluxes and the related physiological processes, we used tower flux measurements from 2003 and 2004 from the QYZ site and the ecosystemmodel EALCO (Ecological Assimilation of Land and Climate Observations). The model explained almost 90%of the variance in the daily LE for both years; whereas 71%and 55% of the variance in H for 2003 and 2004, respectively. Simulations suggested that, during the 2003 summer drought, about 80% of water extraction by roots occurred below 0.45 m, and canopy stomatal conductance decreased in response to soil dryness at a threshold r (relative water content) of 0.5 m. The response of canopy stomatal conductance appeared to be linked to the soil water exposed to themajority of the roots, even if substantialwater was being extracted fromgreater depth. The decrease of canopy stomatal conductance made the ratio of transpiration to ET relatively constant along the years with only a small difference. The different extent of the seasonal drought resulted in different water fluxes for the two years with more distinct influence on evaporation than on transpiration.