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Xiabing Chen,Zhuofei Xu,Lu Li,Huanchun Chen,Rui Zhou 한국미생물학회 2012 The journal of microbiology Vol.50 No.6
Actinobacillus pleuropneumoniae is an important swine respiratory pathogen causing great economic losses worldwide. Identification of conserved surface antigenic proteins is helpful for developing effective vaccines. In this study, a genome-wide strategy combined with bioinformatic and experimental approaches, was applied to discover and characterize surface-associated immunogenic proteins of A. pleuropneumoniae. Thirty nine genes encoding outer membrane proteins (OMPs) and lipoproteins were identified by comparative genomics and gene expression profiling as beinghighly conserved and stably transcribed in the different serotypes of A. pleuropneumoniae reference strains. Twelve of these conserved proteins were successfully expressed in Escherichia coli and their immunogenicity was estimated by homologous challenge in the mouse model, and then three of these proteins (APJL_0126, HbpA and OmpW) were further tested in the natural host (swine) by homologous and heterologous challenges. The results showed that these proteins could induce high titers of antibodies, but vaccination with each protein individually elicited low protective immunity against A. pleuropneumoniae. This study gives novel insights into immunogenicity of the conserved OMPs and lipoproteins of A. pleuropneumoniae. Although none of the surface proteins characterized in this study could individually induce effective protective immunity against A. pleuropneumoniae, they are potential candidates for subunit vaccines in combination with Apx toxins.
Bhuiyan, Maruf A.,Zhou, Hong,Chang, Sung-Jae,Lou, Xiabing,Gong, Xian,Jiang, Rong,Gong, Huiqi,Zhang, En Xia,Won, Chul-Ho,Lim, Jong-Won,Lee, Jung-Hee,Gordon, Roy G.,Reed, Robert A.,Fleetwood, Daniel M. Professional Technical Group on Nuclear Science 2018 IEEE transactions on nuclear science Vol.65 No.1
<P>The radiation hardness of AlGaN/GaN high-electron-mobility transistors (HEMTs) is found to improve with increasing GaN channel thickness. Epitaxial MgCaO shows promise as a radiation-tolerant gate dielectric, with only small shifts in operating parameters of metal–oxide–semiconductor HEMTs observed at doses up to 1 Mrad(SiO<SUB><I>2</I></SUB>). Bias-induced electron trapping and radiation-induced-hole trapping can occur in the MgCaO, depending on the applied bias during stress and/or irradiation. AC transconductance measurements are used to help understand charge trapping in these devices.</P>
( Wenyu Li ),( Fan Yin ),( Zixuan Bu ),( Yuying Liu ),( Yongqing Zhang ),( Xiabing Chen ),( Shaowen Li ),( Lu Li ),( Rui Zhou ),( Qi Huang ) 한국미생물 · 생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.3
Live bacterial vector vaccines are one of the most promising vaccine types and have the advantages of low cost, flexibility, and good safety. Meanwhile, protein secretion systems have been reported as useful tools to facilitate the release of heterologous antigen proteins from bacterial vectors. The twin-arginine translocation (Tat) system is an important protein export system that transports fully folded proteins in a signal peptide-dependent manner. In this study, we constructed a live vector vaccine using an engineered commensal Escherichia coli strain in which amiA and amiC genes were deleted, resulting in a leaky outer membrane that allows the release of periplasmic proteins to the extracellular environment. The protective antigen proteins SLY, enolase, and Sbp against Streptococcus suis were targeted to the Tat pathway by fusing a Tat signal peptide. Our results showed that by exploiting the Tat pathway and the outer membrane-defective E. coli strain, the antigen proteins were successfully secreted. The strains secreting the antigen proteins were used to vaccinate mice. After S. suis challenge, the vaccinated group showed significantly higher survival and milder clinical symptoms compared with the vector group. Further analysis showed that the mice in the vaccinated group had lower burdens of bacteria load and slighter pathological changes. Our study reports a novel live bacterial vector vaccine that uses the Tat system and provides a new alternative for developing S. suis vaccine.
Guanqing Wu,Yongli Xie,Jin Wei,Zengliang Yin,Xiabing Yue 한국강구조학회 2023 International Journal of Steel Structures Vol.23 No.5
The mechanical properties of corrugated steel pipe culverts (CSPCs) for highways depend on the elastic modulus of the foundation soil, the backfill height, and the culvert parameters. Evaluating the stress and deformation characteristics is crucial for the design of CSPCs. A three-dimensional finite element model and field test were used to investigate the effect of various parameters on the earth pressure and deformation of the CSPC during construction. The finite element method results were compared with the test results, and the stress and deformation characteristics of the CSPC were analyzed in detail. As the wavelength increases, the CSPC experiences more deformation, and the earth pressure at the top of the pipe decreases. When the wavelength and thickness are fixed, the displacement of the CSPC decreases, and the earth pressure at the top of the pipe increases with an increase in the wave height of the CSPC. When the wave height and wavelength are fixed, the deformation of the pipe culvert decreases, and the earth pressure at the top of increases with an increase in the pipe culvert’s thickness. These results provide new insights into the construction, design and maintenance of CSPCs.