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      • Playing hardball in a dress? Gender differences in the reliance on and effectiveness of negative campaigns

        Woodall, Gina Marie S Arizona State University 2005 해외박사(DDOD)

        RANK : 2591

        Gender stereotypes are ubiquitous and lead people to perceive men as possessing agentic traits such as boldness and aggressiveness, while women are perceived as possessing communal traits such as warmth and sympathy. In the context of modern political campaigns, where negative campaigning is often viewed as an effective strategy, examining how stereotypes influence the reliance and effectiveness of negative campaigning is important. First, a content analysis was used to examine gender differences in the reliance on negative advertisements in congressional campaigns, using an original dataset. Second, an experiment was conducted on a sample of 370 community volunteers and college students from Arizona State University (ASU) to examine differences in the effectiveness of negative commercials for men and women candidates. Results of the content analysis indicate some gender differences in men and women candidates' reliance on negative advertising, as well as differences between 1998 and 2000. Mainly, female candidates' both played to and dispelled gender stereotypes in 1998, yet only dispelled gender stereotypes in 2000. Results of the experiment also indicate gender differences in how candidates' negative commercials are evaluated. Specifically, when women candidates play to stereotypes in their negative ads, they are more positively evaluated than their male counterparts. Additionally, women are not punished when they dispel gender stereotypes in their negative ads. Furthermore, the gender of the commercial's target is important. Women are advantaged when they run against men and rely on a contrast strategy. Yet, if they rely on an attack strategy, they are only advantaged when running against other women. Finally, the gender of the viewer of the commercials was important. Unexpectedly, women did not reward female candidates for engaging in a negative strategy, but men did. Additionally, there were differences in how students and non-students evaluated the candidates. Students evaluated women more positively when they relied on attack commercials, as opposed to contrast commercials. Yet, non-students evaluated women more positively when they relied on a contrast, rather than an attack strategy. The implications of the dissertation are discussed, with a discussion of whether the double-bind is a real obstacle for women candidates' electorial outcomes.

      • Development of Ion Mobility Spectrometry-Mass Spectrometry Approaches to Study Protein Interactions and Structural Stability

        Woodall, Daniel William ProQuest Dissertations & Theses Indiana University 2020 해외박사(DDOD)

        RANK : 2591

        Ion mobility spectrometry-mass spectrometry (IMS-MS) is a powerful method for characterizing the structures of proteins, as well as interactions that alter their stability. Here we utilize several IMS-MS techniques to probe the effects of interactions with small ligands, other proteins, and chemical modifications on overall protein stability. Multidimensional IMS-MS techniques can provide unique insights into these processes because both structural information and mass information can be determined simultaneously with a high degree of sensitivity in both dimensions. This work is aimed at the development and application of new IMS-MS methodologies to better understand the factors that influence the structural stability of proteins, and their broader role in regulation and function.In the first studies, a variable temperature electrospray ionization (vT-ESI) device was developed for use in conjunction IMS-MS allowing us to generate thermal stability profiles that track changes in the structure and protein mass with increased temperature. This analytical technique was applied to study three protein systems: myohemerythrin, hemoglobin, and myoglobin. The thermal denaturation of myohemerythrin was observed following dissociation of its metal cofactor, involving as many as 18 unique states. Additionally, a non-native disulfide bond was observed to form resulting in a nonfunctional, but thermostable structure that is incapable of proper ligand binding. Analysis of the hemoglobin tetramer provides a measure the stability of quaternary structure and finds evidence that oxidation of a key cysteine residue results in destabilization of the subunit binding interface. In addition to vT-ESI, an infrared laser electrospray source was designed to rapidly heat proteins in nanometer size droplets to probe the early stages of protein unfolding. This droplet heating approach is compared with the vT-ESI approach to investigate myoglobin. These studies find evidence for partially unfolded structures that are trapped in a pre-equilibrium state based on differences in the degree of unfolding observed. Overall, these analytical methods provide a new means of studying protein structural stability in a level of detail that is difficult to achieve by other analytical techniques.

      • Low-Bandwidth Three Dimensional Mapping and Latency Reducing Model Prediction to Improve Teleoperation of Robotic Vehicles

        Woodall, William J., IV Auburn University ProQuest Dissertations & Theses 2012 해외박사(DDOD)

        RANK : 2591

        This thesis uses novel three dimensional sensors like the Microsoft Kinect [1] and the Asus Xtion Pro Live [2] to generate three dimensional environments and use the recon- structed environment with a predictive model in order to assist the teleoperation of mobile vehicles. Ultimately this work would be applicable to any teleoperated vehicle equipped with sensors providing three dimensional data of the environment, such as an automated ATV with a stereo vision system or a Velodyne LiDAR [3] system. The challenges related to utilizing dense three dimensional data in a way that is practical for teleoperation scenar- ios are identified, and solutions are proposed and implemented. To simplify the approach, the problem is split into three smaller tasks: three dimensional mapping, teleoperation and telemetry visualization, and latency reduction techniques. The three dimensional mapping pertains to using the three dimensional sensor data in concert with the mobile vehicle nav- igation solution to generate a three dimensional map of the environment in real-time. The resulting map must be efficiently sent to the teleoperator and visualized in the teleoperation and telemetry visualization section of the thesis. Additionally, latency greatly reduces the teleoperator’s ability to drive the vehicle, so methods for reducing the perceived latency are investigated, including using a vehicle model to simulate the vehicle motion in the absence of timely telemetry updates. It is shown that existing mapping techniques can be used effi- ciently and effective to aid teleoperation, even in low bandwidth environments. Experimental results show that by giving the teleoperator three dimensional information about the envi- ronment, the teleoperator can more successfully navigate tight obstacles and reduce impacts with the environment. Finally, experiments are conducted that show having a prediction of the vehicle motion based on user input can improve teleoperation in high latency situations.

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