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Transcriptome profiling of olive flounder responses under acute and chronic heat stress
Woo‑Jin Kim,이규빈,Dain Lee,Hyun‑Chul Kim,Bo‑Hye Nam,Hyungtaek Jung,이선주,Kyunghwan Kim 한국유전학회 2021 Genes & Genomics Vol.43 No.2
Background The olive founder (Paralichthys olivaceus) is a saltwater fsh, which is valuable to the economy. The olive founder strives to adapt to environmental stressors through physiological, biochemical, and transcriptional responses. The rise in water temperature threatens the growth, development, reproduction, and survival of olive founder. Each organ in the olive founder can diferentially respond to heat stress. Objectives The purpose of this study is to investigate organ-specifc transcriptional changes in olive founder tissues during heat stress. Methods In this study, transcriptome dynamics of the gill, liver, and muscle of olive founder to acute or chronic heat stress were investigated. Results Principal component analysis plotting revealed that the transcriptome of each organ is quite separated. K-means clustering, gene ontology, and Kyoto Encyclopedia of Genes and Genomes pathway analysis showed the diferential transcriptome responses of each organ to heat stress. Heat stress commonly afects the pathways involved in the correct protein folding, DNA repair, and cell cycle. Conclusion Our results may provide a valuable molecular basis of heat acclimation in fshes.
Woo‑Jin Kim,Hee‑Tae Jeong 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.3
In the present work, kinetic equations of dynamic recrystallization and high-temperature deformation mechanism equationswere coupled to calculate and construct processing maps for metallic alloys in 2D and 3D. The validity of this method wasconfirmed for selected materials (Mg and Fe alloys) by comparing the calculated processing maps with those constructedbased on experimental data. The effect of the initial grain size on the processing maps was predicted. The proposed analysismethod is innovative because the processing map for a material of interest can be calculated as a function of strain rate,temperature and strain under any deformation condition once the dynamic recrystallization kinetics and the creep deformationequations for the material are known. Furthermore, the proposed method can provide information regarding the ratecontrollingcreep deformation mechanism and microstructural evolution at any location on the processing maps.