대학태권도선수의 실수인식과 의도적 연습 및 인지된 경기력의 관계

최봉준(Choi, Bong Jun),원세종(Won, Se-Jong) 세계태권도문화학회 2022 세계태권도문화학회지 Vol.13 No.2

The purpose of this study was to investigate the relationship of perception of error, deliberate practice, and perceived performance of University Taekwondo Athletes. To this end, 321 Taekwondo athletes (male 207, female 114) participated in this study. Collected data were analyzed by descriptive statistics, correlation, and multiple regression using SPSS 22.0 version. The results revealed as follow. First, learning from error, error risk taking, and thinking about error had a positive effect on deliberate practice. Second, learning from error and thinking about error had a positive effect on performance satisfaction while error risk taking and thinking about error had a positive effect on will to win. Third, deliberate practice had a positive effect on performance satisfaction and will to win. In conclusion, athletes’ perception of error is key component to enhance deliberate practice and perceived performance. Furthermore, deliberate practice was also identified as a variable that improves perceived performance.

GG-38 : Minimal invasive surgery (LESS) for huge cystic adnexal tumors in whole abdomen

( In Young Choi ),( Ji Hye Lee ),( Mi Hyun Park ),( Ji Kwoun Park ),( Jeong Kyu Shin1 ),( Won Jun Choi1 ),( Soon Ae Lee ),( Jong Hak Lee ),( Won Young Paik ) 대한산부인과학회 2014 대한산부인과학회 학술대회 Vol.100 No.-

Laparoendoscopic single-site surgery (LESS) is helpful if not an evidence of suspicious malignancy in huge adnexa tumors. This approach might be reasonable alternative to conventional laparoscopic surgery. We report two cases with LESS for huge cystic adnexal tumors in whole abdomen. A 58 years old multigravida woman in menopause underwent removal of a huge cystic adnexal mass that occupied her entire abdomen by LESS.(ca.5200ml aspiration) A 15 years old single nulligravida woman had a huge cystic adnexal tumor in whole abdomen reaching to the level of the xyphoid process. A needle through the transumbilical single port was inserted directly under the mass. Subsequently, 8000 ml of cystic fluid was aspirated without spillage.

형질전환 제초제저항성 벼 밀양 204호의 농업적 특성

정응기,이기환,원용재,박향미,전남수,최준호,구연충,한창덕,은무영,김태산,남민희,Jeong Eung-Gi,Yi Gi-Hwan,Won Yong-Jea,Park Hyang-Mi,Cheon Nam-Soo,Choi Jun-Ho,Ku Yeon-Chung,Han Chang-Deok,Eun Mu-Yeong,Kim Tae-Sas,Nam Min-Hee 한국식물생명공학회 2005 식물생명공학회지 Vol.32 No.2

밀양 204호는 작물과학원 영남농업연구소 생명공학연구팀이 직파적응 제초제저항성 벼 품종을 육성할 목적으로 '98년 bar 유전자를 동진벼에 아그로박테리움법으로 형질 전환하여 제초제저항성 벼를 양성하였다. '98/'99년 동계에 단간 내도복 양질인 주남벼를 인공교배하여 약배양 등 육종프로그램에 적용하여 우량계통을 선발하고 밀양 204호로 계통명을 부여하였다. GM 벼와 Non-GM 벼의 농업적 특성을 UPOV 및 국립종자관리소의 품종등록 기준에 따른 농업적특성 중 차이가 있었던 것은 엽색도, 지엽의 형태, 간장, 수당립수 등 이었다. This study was conducted to investigated the major characteristics of genetically modified rice of 'Milyang 204' originated from Dongjinbyeo compared to a non-transgenic rice varieties Dongjinbyeo and Jun-ambyeo. Basta resistant transgenic rice lines carrying bar gene produced by the Yeongnam Agricultural Research Institute were evaluated for their agronomic characters. The transgenic Japonica rice of 'Milyang 204' showed inferior phenotypic traits compared to a non-transgenic rice variety Dongjinbyeo and Junambyeo. On the basis of UPOV (Union Internationale Pour la Protaection des Obtentions Vegetables) and NSMO(National Seed Management Office) the transgenic 'Milyang 204' showed difference in some traits out of some agronomic traits, such as leaf color, angle of flag leaf, number of spikelets, culm length, white core and white belly compared to the non-transgenic varieties rice.

In-depth proteomic analysis of human bronchoalveolar lavage fluid towards the biomarker discovery for lung cancers

( Jun-hyung Lee ),( Jin-young Kim ),( Min-sik Kim ),( Seung Hyeun Lee ),( Jung Mi Lee ),( Myung Jae Park ),( Won Gun Kwack ),( Kyoung-hee Sohn ),( Hye Sook Choi ) 대한결핵 및 호흡기학회 2019 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.127 No.-

Background: Since BALF were directly collected from the tumor environment than plasma, we expected that biomarkers for the lung cancer may exist in higher concentration in BALF than in blood. Here, we developed a novel method for in-depth proteomic analysis of BALF by combining antibody-based depletion of high abundant proteins from BALF with high pH pep tide fractionation. Material and Methods; BALF samples were collected from segmental bronchus from 14 patients with lung cancers, which were centrifuged at 500 g for 5 minutes at 4°C to remove debris. Two milliliters from each of 14 patients were pooled and concentrated using Amicon filters at 5,000 g for 60 min at 4°C. Depletion was performed to remove 14 high abundant proteins Then, high pH reversed phase liquid chromatography fractionation was carried out using UltiMate 3000 HPLC. Peptides were analyzed on a high resolution Orbitrap Fusion mass spectrometer. Results: MaxQuant search resulted in the identification of 4,615 protein groups, from which 3,786 proteins were in common between lung tumors and BALF from lung cancer patients. Interestingly, 748 were specifically enriched in BALF as compared to lung tumors. We found that 28 potential biomarker proteins make up ~26% of protein concentration in BALF while ~14% in human plasma. Conclusion: We developed a novel proteomic approach that combined antibody-based depletion with high pH RPLC fractionation to maximize the BALF protein identification from lung cancer patients. Our result implied that BALF contains concentrated lung cancer biomarkers and suggests a possibility that BALF is a better source than plasma for the diagnosis of the lung cancer. We believe that this method help find novel biomarkers for the early diagnosis of the lung cancer and would facilitate the lung cancer research.

Physicochemical Characteristics of Various Peach Cultivars

Jun-Young Lee,Heui-Dong Park,Sang-Won Choi 한국식품영양과학회 2001 Preventive Nutrition and Food Science Vol.6 No.2

Physical and chemical characteristics of four white- and one yellow-fleshed peach cultivars were compared at optimum maturity stage. In addition, differences of phenolic composition, polyphenol oxidase (PPO) activities and PPO isozyme patterns in two peach types were also investigated. There are no significant differences in firmness and color values between two peach types, except for "Yumyung", a white-fleshed peach with the highest firmness, and "Hwangdo", a yellow-fleshed peach with the highest yellowness (b^*) value. In general, the soluble solid/titratable acidity ratios, ascorbic acid and total phenolic contents were significantly higher for white-fleshed peaches than those for yellow-fleshed peach (p<0.05). Three major phenolic compounds, catechin, neochlorogenic acid and chlorogenic acid, were found in both the white- and yellow-fleshed peaches. Among them, catechin was the predominant phenolic compound in the white-fleshed peaches, followed by neochlorogenic acid, and chlorogenic acid. Meanwhile, neochlorogenic acid was present in the highest level of the yellow-fleshed peach, but levels of two other phenolic compounds were lower. PPO activities of the white-fleshed peaches were generally higher than that of the yellow-fleshed peach, with the exception of "Yumyung". The partially purified PPO of two peach cultivars have together three active bands including one major band and two minor bands, and there were no big differences in PPO isozyme patterns between two different peach cultivars.

Elimination of Saturated Fatty Acids, Toxic Cyclic nonapeptide and Cyanogen Glycoside Components from Flax Seed Oil

Choi, Eun-Mi,Kim, Jeung-Won,Pyo, Mi-Kyung,Jo, Sung-Jun,Han, Byung-Hoon The Korean Society of Applied Pharmacology 2007 Biomolecules & Therapeutics(구 응용약물학회지) Vol. No.

Flax seed(Linseed, Linum usitatissimum L.) and its oil, a richest source of alpha-linolenic acid(ALA)(${\omega}-3$), contain saturated fatty acids, neurotoxic cyanogen glycosides and immuno-suppressive cyclic-nonapeptides. Present paper describes the development of two chemical processes, Process-A and -B, to remove saturated fatty acids and to destroy cyclic nonapeptides and cyanogen glycosides from flax seed oil. Process-A consists of three major steps, i.e., extraction of fatty acid mixture by alkaline saponification, removal of saturated fatty acid by urea-complexation, and triglyceride reconstruction of unsaturated fatty acid via fatty acyl-chloride activation using oxalyl chloride. Process-B consists of preparation of fatty acid ethyl ester by transesterification, elimination of saturated fatty acid ester by urea-complexation, and reconstruction of triglyceride by interesterification with glycerol-triacetate (triacetin). The destruction of lipophilic cyclic nonapeptide during saponification or transesterification processes could be demonstrated indirectly by the disappearance of antibacterial activity of bacitracin, an analogous cyclic-decapeptide. The cyanogen glycosides were found only in the dregs after hexane extraction, but not in the flax seed oil. The reconstructed triglyceride of flax seed oil, obtained by these two different pathways after elimination of saturated fatty acid and toxic components, showed agreeable properties as edible oil in terms of taste, acid value, iodine and peroxide value, glycerine content, and antioxidant activity.

Changes in Flavonoid Contents of Safflower Leaf during Growth and Processing

Jun-Young Lee,Kyeng-Seok Park,Sang-Won Choi 한국식품영양과학회 2005 Preventive Nutrition and Food Science Vol.10 No.1

Eight flavonoids, apigenin-6-C-β-D-glucopyranosyl-8-C-β-D-glucopyranoside (AGG), quercetin 7-O-β-Dglucopyranoside (QG), luteolin 7-O-β-D-glucopyranoside (LG), quercetin 7-O-(6"-O-acetyl)-β-D-glucopyranoside (QAG), luteolin 7-O-(6"-O-acetyl)-β-D-glucopyranoside (LAG), quercetin (Q), luteolin (L) and acacetin 7-O-β-D-glucuronide (AG) were determined by HPLC in the safflower (Carthamus tinctorius L.) leaf during growth and processing. During growth, levels of five flavonoid glycosides (AGG, QG, LG, QAG, & LAG) in the leaf increased progressively at over time according to growth stages, reached a maximum before June 11, and then decreased sharply, while those of three flavonoid aglycones (Q, L, & AG) increased greatly at the early stage of growth, reached a peak before May 28, and then decreased rapidly. During the steaming process, contents of five flavonoid glycosides increased rapidly with increased steaming time, reached a maximum after 5 min of steaming, and then decreased, whereas those of flavonoid aglycones except for AG decreased sharply with increased steaming time. During the roasting process, contents of three flavonoid glycosides decreased rapidly with increased roasting time, whereas those of two acetylflavonoid glycosides (QAG & LAG) and three flavonoid aglycones increased progressively with increased roasting time, reached a maximum after 3 min of roasting, and then decreased. These results suggest that appropriate steamed and roasted safflower leaves are a rich source of flavonoids, and may be a good source of bioactive components as a functional leaf tea.

Construction of Sucrose-utilizing Escherichia coli K-12 Strain by Cloning β-fructofuranosidases

Won Jun KIM,Sol CHOI,Jeong Wook LEE,Sang Yup LEE 한국생물공학회 2012 한국생물공학회 학술대회 Vol.2012 No.4

Evaluation of Two Types of Biosensors for Immunoassay of Botulinum Toxin

Choi, Ki-Bong,Seo, Won-Jun,Cha, Seung-Hee,Choi, Jung-Do Korean Society for Biochemistry and Molecular Biol 1998 Journal of biochemistry and molecular biology Vol.31 No.1

Immunoassay of botulinum toxin (BTX) B type was investigated using two typed of biosensors: light addressable potentiometric sensor (LAPS) and surface plasmon resonance (SPR) sensor. Urease-tagged and immuno-filtration capture method have been used for LAPS. Tag-free and direct binding real-time detection method have been used for SPR sensor. The detection limit of sandwich assay format with LAPS was 10 ng/ml, which was the lowest among methods tested. SPR has the advantage of being more convenient because tag-free direct binding assay can be used and reaction time was reduced, regardless of low sensitivity. This result shows that sandwich assay format with LAPS can be used as an alternative method of BTX mouse bioassay which is known as the most sensitive method for the detection of BTX.

수행평가에 대한 교사 이야기

(Won Jun Choi),(Jin Hee Kim) 한국체육학회 2001 The 2001 Seoul International Sport Science Congres Vol.- No.-