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Efficient Plant Regeneration from Shoot Tip and Young Leaf in Rhodiola sachalinensis A. Bor.
Chi,Hyung-Joon,Yoon,Jae-Ho,Yang,Deok-Chun,Song,Won-Seob 한국자원식물학회 2003 Plant Resources Vol.6 No.3
The shoot tip and young leaf of Rhodiola sachalinensis were cultured to invest the plant growth regulator condition for callus induction, shoot and root regeneration. When the shoot tip was sterilized in 2.0% of NaOCl for 20min., the contamination rate was the lowest. And the survival rate of the culture material was good in carbenicillin 500mg/L treatment group. Callus was obtained from shoot tip and young leaf segments. NAA 0.1-1.0mg/L and 2,4-D 0.1-0.5mg/L alone treatment were shown to have a good response on callus induction from shoot tip culture. In the case of young leaf culture, NAA and 2,4-D 0.1-0.5mg/L alone treatment were good in callus induction. In culturing shoot tip NAA 0.5mg/L and BA 0.5mg/L, NAA1.0mg/L and BA 0.lmg/L combination treatment was good in shoot regeneration. The regenerated shoots were rooted on MS medium supplemented with NAA and BA combination treatment. Especially, NAA 1.0mg/L and BA 0.1mg/L combination treatment was effective for root regeneration.
Hwang, Chi-Young,Kim, Gi Heon,Yang, Jong-Heon,Hwang, Chi-Sun,Cho, Seong M.,Lee, Won-Jae,Pi, Jae-Eun,Choi, Ji Hun,Choi, Kyunghee,Kim, Hee-Ok,Lee, Seung-Yeol,Kim, Yong-Hae The Royal Society of Chemistry 2018 Nanoscale Vol.10 No.47
<P>Correction for ‘Rewritable full-color computer-generated holograms based on color-selective diffractive optical components including phase-change materials’ by Chi-Young Hwang <I>et al.</I>, <I>Nanoscale</I>, 2018, DOI: 10.1039/c8nr04471f.</P>
원을지(Ul Chi Won),이선재(Sun Jae Lee) 한국복식학회 1999 服飾 Vol.46 No.-
The fashion advertisement is communication method between clothing and consumer. The development of mass communication accelerated that In modern society Post-modernism advertisement is a specialized and separated form so it confuses the consumers who are used in traditional AD. The peculiarities (non-form, destruction of description, compound of genre, mixing of reality and fiction) are more reflected in fashion advertisement. The sample consisted of 425 mail and female and the results were analyzed using frequency and percentage calculation, Close tabs, T-Test, Oneway Anova, Factor analysis, Chi-square Test, Regression, Correlation Analysis. The result of this study were as follows: The post-modernism fashion ad is the separated and reflected form for young age`s favorite. By the factor analysis of consumer response in each 5 emotional factors proved to be valid ones(fine, negative, sexual, stimulative, constructive aspects). The fine and negative aspects of the factors are very concerned with the consumer`s favorite attitude of fashion ad. There is no significant difference in age, job but sex has a significant difference. In according to the method of the ad expression consumer`s acceptance is difference in sex. There were no significant concern with excess effects and purchase intend in fashion advertisement.
( Won-young Kim ),( Seungyong Park ),( Hwa Jung Kim ),( Moon Seong Baek ),( Chi Ryang Chung ),( So Hee Park ),( Byung Ju Kang ),( Jin Young Oh ),( Woo Hyun Cho ),( Yun Su Sim ),( Young-jae Cho ),( Sun 대한결핵 및 호흡기학회 2019 Tuberculosis and Respiratory Diseases Vol.82 No.3
Background: Beyond its current function as a rescue therapy in acute respiratory distress syndrome (ARDS), extracorporeal membrane oxygenation (ECMO) may be applied in ARDS patients with less severe hypoxemia to facilitate lung protective ventilation. The purpose of this study was to evaluate the efficacy of extended ECMO use in ARDS patients. Methods: This study reviewed 223 adult patients who had been admitted to the intensive care units of 11 hospitals in Korea and subsequently treated using ECMO. Among them, the 62 who required ECMO for ARDS were analyzed. The patients were divided into two groups according to pre-ECMO arterial blood gas: an extended group (n=14) and a conventional group (n=48). Results: Baseline characteristics were not different between the groups. The median arterial carbon dioxide tension/ fraction of inspired oxygen (FiO<sub>2</sub>) ratio was higher (97 vs. 61, p<0.001) while the median FiO<sub>2</sub> was lower (0.8 vs. 1.0, p<0.001) in the extended compared to the conventional group. The 60-day mortality was 21% in the extended group and 54% in the conventional group (p=0.03). Multivariate analysis indicated that the extended use of ECMO was independently associated with reduced 60-day mortality (odds ratio, 0.10; 95% confidence interval, 0.02-0.64; p=0.02). Lower median peak inspiratory pressure and median dynamic driving pressure were observed in the extended group 24 hours after ECMO support. Conclusion: Extended indications of ECMO implementation coupled with protective ventilator settings may improve the clinical outcome of patients with ARDS.
Blinking Heart : Pneumopericardium
( Jae Won Oh ),( Chi Young Shim ),( Ho Youn Won ),( Byeong Keuk Kim ),( Seok Min Kang ) 대한내과학회 2011 대한내과학회 추계학술대회 Vol.2011 No.1
A 56-year-old man presented with chest discomfort on emergency room. He had stroke 8 years ago and right upper lobectomy (lung cancer, adenocarcinoma) with lymph node dissection 2 weeks ago. Electrocardiogram revealed normal sinus rhythm without any ischemic signs but chest radiograph showed pleural effusion at right lobe and pneumopericardium at left heart border (Figure 1). Computed tomography (CT) showed pneumopericardium at anterior side but no sign of bronchopericardial fistula (Figure 2). Two-dimensional transthoracic echocardiogram was visible only in diastolic phase (Figure 3) but hindered by trapped pericardial air in systolic phase (Figure 4). It could be ``blinking heart sign` in echocardiogram of pneumopericardium, especially in early phase at parasternal long view and parasternal short view. We could not find any compromised-hemodynamic sign and remarkable abnormality. Chest tube was inserted for drainage of right pleural effusion and pleural fluid analysis revealed chylothorax. So we presumed that pneumopericaridum resulted from the sequela of post lung cancer operation. During conservative care with chest tube drainage and intermittent nasal oxygen therapy, his symptoms did not aggravate and his vital signs were stable. After improvement in pleural effusion and pneumopericardium, chest tube was removed. The patient was discharged in good condition and has subsequently done well.
Chi, Won-Jae,Kim, Yoon-Hee,Kim, Jong-Hee,Kang, Dae-Kyung,Kang, Sang-Soon,Suh, Joo-Won,Hong, Soon-Kwang The Microbiological Society of Korea 2003 The journal of microbiology Vol.41 No.4
Gelatinase is a proteolytic enzyme that hydrolyzes gelatin. Gelatinolytic activity was detected from culture broths of Streptomyces griseus IFO13350 and HH1 by paper disc assays on 0.5% agar plates containing 1% gelatin. The concentrated extracellular protein from the S. griseus was analyzed by SDS polyacrylamide gel, and two proteins, with molecular weights of 30 and 28 kDa, respectively, were identified to have gelatinase activity by gelatin zymography. The protein with a molecular weight of 28 kDa was confirmed to be S. griseus trypsin (SGT). The effects of metal ions and metal chelators on the protease activity of the SGT were studied. Of the metal ions tested, only manganese was found to enhance the protease activity, 2.6 times, however, $Co^{2+},\;Cu^{2+},\;and\;Zn^{2+}$, and metal chelators, such as EDTA and EGTA, inhibited the SGT activity. When the protease activity of the SGT was measured at various pHs, in the presence of 5 mM $MnCl_2$, its highest activity was at pH 11.0, whereas only 60% of the maximum activity was observed between pHs 4.0 and pH 6.0, and almost 80% activity between pHs 7.0 to pH 10.0. The protease activity was measured at various temperatures in the presence of 5 mM $MnCl_2$. The SGT was found to be stable up to $60^{\circ}C$ for 30 min, while only 16% of the enzyme activity remained at $60^{\circ}C$, and at $80^{\circ}C$ almost all the activity was lost. The optimal temperature for the protease activity was $50^{\circ}C$.
Won, Ki-Bum,Chang, Hyuk-Jae,Niinuma, Hiroyuki,Sung, Jimin,Shin, Sanghoon,Cho, In-Jeong,Shim, Chi-Young,Hong, Geu-Ru,Kim, Young Jin,Choi, Byung-Wook,Chung, Namsik Lippincott WilliamsWilkins 2015 Coronary artery disease Vol.26 No.2
<P><B>Background</B></P><P>Compared with coronary computed tomographic angiography (CCTA), the coronary artery calcium score (CACS) may be limited in predicting adverse cardiovascular events in asymptomatic diabetic patients.</P><P><B>Methods</B></P><P>We evaluated the predictive value of CACS for obstructive coronary plaques (OCPs) assessed by CCTA in 328 consecutive asymptomatic patients with type 2 diabetes mellitus who had an estimated glomerular filtration rate greater than 60 ml/min/1.73 m<SUP>2</SUP>.</P><P><B>Results</B></P><P>In total, 29 (9%) patients had OCPs: calcified or mixed OCPs and noncalcified OCPs were found in 26 (8%) and three (1%) patients, respectively. On the basis of a CACS of 0, 1–10, 11–100, and greater than 100, OCPs were found in 2, 5, 15, and 36% of patients, respectively. On receiver operating characteristic curve analysis, the optimal cutoff CACS for predicting OCPs was found to be 33, with 83% sensitivity and 81% specificity (area under the curve, 0.853; 95% confidence interval, 0.777–0.930; <I>P</I><0.001). Positive and negative predictive values of a CACS of 33 for OCPs were 30 and 98%, respectively. On multivariate logistic regression analysis, age [odds ratio (OR), 1.09], microalbuminuria (OR, 3.43), current smoking (OR, 3.93), and CACS greater than 33 (OR, 15.85) were found to be independently associated with an increased risk for OCPs (<I>P</I><0.05, respectively). The predictive significance of CACS greater than 33 for OCPs improved after considering the status of microalbuminuria and current smoking.</P><P><B>Conclusion</B></P><P>These findings suggest that CACS is an effective gatekeeper for evaluating obstructive coronary artery disease using CCTA in asymptomatic Korean patients with type 2 diabetes mellitus who have near-normal kidney function.</P>