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Surface plasmon remonance based single-walled carbon nanotube detection
Won-Hwi Na(나원휘),Dae-Ho Jang(장대호),Nam-jun Kim(김남준),Se-Hyun Shin(신세현) 대한기계학회 2015 대한기계학회 춘추학술대회 Vol.2015 No.11
In this study, we developed detection method of single-walled carbon nanotubes (SWNTs) in an aqueous solution using surface plasmon resonance (SPR) spectroscopy. A strong affinity conjugation with biotin-streptavidin was adopted for a sensitive and specific detection on a sensing mechanism. During the pretreatment process, the SWNT surface was functionalized and hydrophilized using a thymine-chain based biotinylated single-strand DNA linker. In addition, bovine serum albumin was added to enhance the degree of dispersion of SWNT. The pretreated SWNTs were captured on a sensing sensor chip surface by streptavidins immobilized on biotilylated gold sensing film. The captured SWNTs were measured in real-time using SPR spectroscopy. By our detection method using SPR sensor, we achieved the limit of detection as 100 fg/ml and the SPR sensor showed a linear characteristic within the range of 100 pg/ml to 200 ng/ml.