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Mansee, Ayman H.,Chen, Wilfred,Mulchandani, Ashok The Korean Society for Biotechnology and Bioengine 2000 Biotechnology and Bioprocess Engineering Vol.5 No.6
Recently, we reported an improved technology for the degradation of organophosphate nerve agents using whole cells of genetically engineered Escherichia coli that anchored and displayed the enzyme organophosphorus hydrolase on the cell surface. In this paper we report the immobilization of these cells on highly porous sintered glass beads and the subsequent application of the immobilized cell in a continuous-flow packed bed bioreactor for the biodetoxification of a widely used insecticide, coumaphos.
Magnetically Assembled Multisegmented Nanowires and Their Applications
Bangar, Mangesh A.,Hangarter, Carlos M.,Yoo, Bongyoung,Rheem, Youngwoo,Chen, Wilfred,Mulchandani, Ashok,Myung, Nosang V. WILEY-VCH Verlag 2009 Electroanalysis Vol.21 No.1
<P>Here we report a cost effective and versatile way of synthesizing and assembling multi-functional (e.g., gold-polypyrrole-nickel-gold) nanowires. Multisegmented nanowires were synthesized using electrodeposition method for precise control over segment dimensions for proper expression of material functionality. The nanowires were integrated on microfabricated electrodes using magnetic dipole interactions between the ferromagnetic segment in the nanowire and the ferromagnetic electrodes. The electrical properties of multisegmented nanowires showed semiconducting behavior with the activation energy of ca. 0.27 eV for the conducting polymer segment of the nanowire. These nanowire devices showed response towards light and exposure to ammonia, demonstrating their potential use as photonic device and gas sensor, respectively.</P>
Growth Mechanism of Amorphous Selenium Nanoparticles Synthesized by <i>Shewanella</i> sp. HN-41
TAM, Kawai,HO, Cuong Tu,LEE, Ji-Hoon,LAI, Min,CHANG, Chong Hyun,RHEEM, Youngwoo,CHEN, Wilfred,HUR, Hor-Gil,MYUNG, Nosang V. Japan Society for Bioscience, Biotechnology, and A 2010 Bioscience, Biotechnology, and Biochemistry Vol.74 No.4
<P><I>Shewanella</I> sp. HN-41 was exploited for selenium nanoparticles synthesis from aqueous selenite compounds under anaerobic conditions. Various reaction conditions, including reaction time, initial biomass, and initial selenite concentration, were systematically investigated to determine their effects on particle size distribution and formation rate. The biomass concentration of <I>Shewanella</I> sp. HN-41 had no significant effect on average particle size but strongly influenced reduction rate and size distribution. Initial selenite concentration (0.01–1.0 m<SMALL>M</SMALL>) also had no significant effect on the average particle size, but affected the early growth stage mechanism of selenium particle production, which was modeled using a Michaelis Menten model. The HR-TEM and SAED patterns indicated that the synthesized selenium nanoparticles were amorphous.</P>