http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Tae Hwa Kang,Sang Hun Han,Hang Yeon Weon,Young Bo Lee,Namjung Kim,Seong Hyeon Kim,Weon Tae Kim,Gwan Ho Park,Seong Hee Nam,Hae Chul Park 한국응용곤충학회 2012 한국응용곤충학회 학술대회논문집 Vol.2012 No.10
Allomyrina dichotoma is a typical pet beetles in Korea. From 2010, similar symptoms of milky spore disease were found collectively in grubs of the species reared in insect farms. They shared a specific symptom that the skin of last instar larvae was changed softer with opaque white and infested grubs eventually died. To clarify the cause of the symptom, we collected the larvae of A. dichotoma from five farms and examined intestinal bacterial florae of them using pyrosequencing technique. From those results, a member of Paenibacillus was found only in the larvae showing the symptom of disease. Through PCR analysis using a Paenibacillus specific primer set, we obtained the partial 16S rRNA gene sequence and confirmed the microbe as Paenibacillus sp. For detailed characterizing, a whole guts was extracted from each larva showing the sign of the disease and incubated at 70℃ for 15 min to isolate spore forming bacteria. After then, each content of guts was cultured on MYPGPNAL agar medium (12.5 μg/ml of nalidixic acid) at 30℃. The 16S rRNA gene sequence analysis for six isolate showed that they were closely related to P. rigui (97.5~98.0% similarity) and to P. chinjuensis (95.9~96.6% similarity). Additional tests including API test and cellular fatty acid composition analysis were performed, but the strain couldn’t be identified at species level, suggesting it may represent novel species of the genus Paenibacillus.
Kang, Tae Hwa,Han, Sang Hoon,Weon, Hang Yeon,Lee, Young Bo,Kim, Namjung,Nam, Sung Hee,Park, Hae Chul Korean Society of Sericultural Science 2012 International Journal of Industrial Entomology Vol.25 No.2
In reared populations of Allomyrina dichotoma, commercial insects, the skin of last instar larvae was changed softer with opaque white, and infested grubs eventually died. To clarify the cause of the symptom, we collected the larvae of A. dichotoma from five farms and examined their intestinal bacterial florae using pyrosequencing technique. From those results, a member of Paenibacillus was found only in the larvae showing the symptom of disease. Through PCR analysis using a Paenibacillus specific primer set, we obtained the partial 16S rRNA gene sequence and confirmed the microbe as Paenibacillus sp. For clear identification, a whole guts was extracted from each larva showing the sign of the disease and incubated at $70^{\circ}C$ for 15 min to isolate spore forming bacteria. After then, each content of guts was cultured on $MYPGP_{NAL}$ agar medium($12.5{\mu}g/ml$ of nalidixic acid) at $30^{\circ}C$. The 16S rRNA gene sequence analysis for the isolated bacteria showed that they were closely related to P. rigui(97.9% similarity), to P. chinjuensis(96.1% similarity), and to P. soli(95.3% similarity). Additional tests including API test and cellular fatty acid composition analysis were performed, but the strain couldn't be identified at species level, suggesting it may represent novel species of the genus Paenibacillus.
정화영(Hwa-Young Jung),김진천(Jin-Cheon Kim),강기원(Ki-Weon Kang) 대한기계학회 2008 대한기계학회 춘추학술대회 Vol.2008 No.5
Because of their superior performance and significant weight savings, the fiber reinforced composite materials have been widely used in the wide range of industry. In particular, for the naval structures, the weight reduction is critical design requirement and hence the development and design of composite structures is utmost important issue. In this study, a statistical analysis and a series of static tests are performed to identify the distribution of strength of carbon/epoxy composite material and to establish the design criteria for composite structures.
정화영(Hwa-Young Jung),김태성(Tae-Sung Kim),권용규(Yong-Kyu Kwon),한우섭(Woo-Sup Han),강기원(Ki-Weon Kang) 대한기계학회 2010 대한기계학회 춘추학술대회 Vol.2010 No.11
This paper presents the probabilistic analysis for fatigue life of Glass/Epoxy laminates with impact-induced damage. For this, a series of impact tests were performed using instrumented impact testing machine. Then, tensile and fatigue tests carried out so as to generate post-impact residual strength and fatigue life. Two Parameter Weibull distribution was used to fit the residual strength and fatigue life data of composite laminates. The variance of residual strength decreased with increasing of impact energy. The fatigue life of impacted laminates was greatly reduced by impact energy and this trend depended on applied stress amplitude. Also, the variation of fatigue life was gradually decreased with the applied stress amplitude.
( Tae Hwa Kang ),( Sang Hoon Han ),( Hang Yeon Weon ),( Young Bo Lee ),( Namjung Kim ),( Sung Hee Nam ),( Hae Chul Park ) 한국잠사학회 2012 International Journal of Industrial Entomology Vol.25 No.2
In reared populations of Allomyrina dichotoma, commercial insects, the skin of last instar larvae was changed softer with opaque white, and infested grubs eventually died. To clarify the cause of the symptom, we collected the larvae of A. dichotoma from five farms and examined their intestinal bacterial florae using pyrosequencing technique. From those results, a member of Paenibacillus was found only in the larvae showing the symptom of disease. Through PCR analysis using a Paenibacillus specific primer set, we obtained the partial 16S rRNA gene sequence and confirmed the microbe as Paenibacillus sp. For clear identification, a whole guts was extracted from each larva showing the sign of the disease and incubated at 70oC for 15 min to isolate spore forming bacteria. After then, each content of guts was cultured on MYPGPNAL agar medium(12.5 μg/ml of nalidixic acid) at 30oC. The 16S rRNA gene sequence analysis for the isolated bacteria showed that they were closely related to P. rigui(97.9% similarity), to P. chinjuensis(96.1% similarity), and to P. soli(95.3% similarity). Additional tests including API test and cellular fatty acid composition analysis were performed, but the strain couldn`t be identified at species level, suggesting it may represent novel species of the genus Paenibacillus.