A Proteomic Analysis of Leaf Responses to Enhanced Ultraviolet-B Radiation in Two Rice (Oryza sativa L.) Cultivars Differing in UV Sensitivity

Xing-Chun Wu,Chang-Xun Fang,Jin-Yang Chen,Qing-Shui Wang,Ting Chen,Wen-Xiong Lin,Zhong-Liang Huang 한국식물학회 2011 Journal of Plant Biology Vol.54 No.4

To determine the proteomic response to UV irradiation, two cultivars, i.e., Lemont (UV tolerant) and Dular (UV sensitive), were exposed to natural and enhanced ultraviolet-B (UV-B) irradiation for 1, 7, and 14 days, and two-dimensional gel electrophoresis in combination with mass spectrometry (MS) and bioinformatics were used to compare the different proteomic responses in the leaves of the two cultivars. Thirty-nine proteins were up- or downregulated following the UV-B treatments. Among them, 30 increased or decreased more than 1.5-fold in abundance. They were further tested by using matrix-assisted laser desorption/ionization time of flight MS and performed a database search. Twentyfour proteins were thus identified. These identified proteins were mostly upregulated in Lemont, whereas only 14 of them upregulated in Dular. Nine proteins involved in glycometabolism and fatty acid metabolisms, signal transduction, and protein synthesis and folding in Dular were not changed. These results suggest that there was a complex regulative mechanism on the proteomes in rice leaves upon UV-B exposure.

Mechanical Error Analysis of Disk Cam Mechanisms with a Flat-Faced Follower

Wen-Tung Chang,Long-Iong Wu 대한기계학회 2006 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.20 No.3

By employing the concept of equivalent linkage, this paper presents an analytical method for analyzing the mechanical errors of disk cam mechanisms with a flat-faced follower. The resulting error equations do not really involve the location of the curvature center of the cam profile, and locating the curvature center of the cam profile is not essential. The resulting errors are significantly affected by the pressure angle, and the smaller pressure angle will result in the smaller mechanical error. In the worst case, owing to the joined effects of various design parameters, the accuracy of the follower motion may degrade considerably. For the oscillating follower case, all acceleration error functions have a sudden change at every beginning and at every end of the motion even though the theoretical follower displacement is cycloidal motion.

Enhancement of Aggregation-Induced Emission in Dye-Encapsulating Polymeric Micelles for Bioimaging

Wu, Wen-Chung,Chen, Ching-Yi,Tian, Yanqing,Jang, Sei-Hum,Hong, Yuning,Liu, Yang,Hu, Rongrong,Tang, Ben Zhong,Lee, Yi-Ting,Chen, Chin-Ti,Chen, Wen-Chang,Jen, Alex K.-Y. WILEY-VCH Verlag 2010 Advanced Functional Materials Vol.20 No.9

<P>Three amphiphilic block copolymers are employed to form polymeric micelles and function as nanocarriers to disperse hydrophobic aggregation-induced emission (AIE) dyes, 1,1,2,3,4,5-hexaphenylsilole (HPS) and/or bis(4-(N-(1-naphthyl) phenylamino)-phenyl)fumaronitrile (NPAFN), into aqueous solution for biological studies. Compared to their virtually non-emissive properties in organic solutions, the fluorescence intensity of these AIE dyes has increased significantly due to the spatial confinement that restricts intramolecular rotation of these dyes and their better compatibility in the hydrophobic core of polymeric micelles. The effect of the chemical structure of micelle cores on the photophysical properties of AIE dyes are investigated, and the fluorescence resonance energy transfer (FRET) from the green-emitting donor (HPS) to the red-emitting acceptor (NPAFN) is explored by co-encapsulating this FRET pair in the same micelle core. The highest fluorescence quantum yield (∼62%) could be achieved by encapsulating HPS aggregates in the micelles. Efficient energy transfer (>99%) and high amplification of emission (as high as 8 times) from the NPAFN acceptor could also be achieved by spatially confining the HPS/NPAFN FRET pair in the hydrophobic core of polymeric micelles. These micelles could be successfully internalized into the RAW 264.7 cells to demonstrate high-quality fluorescent images and cell viability due to improved quantum yield and reduced cytotoxicity.</P> <B>Graphic Abstract</B> <P>Highly efficient fluorescence probes are achieved through the encapsulation of aggregation-induced emission molecules, 1,1,2,3,4,5-hexaphenylsilole (HPS) and/or bis(4-(N-(1-naphthyl) phenylamino)-phenyl)fumaronitrile (NPAFN) in the core of polymeric micelles. Bright fluorescence cell images are shown with tunable colors of green directly from HPS aggregates and red through efficient fluorescence resonance energy transfer (FRET) from HPS aggregates to NPAFN aggregates. <img src='wiley_img_2010/1616301X-2010-20-9-ADFM200902043-content.gif' alt='wiley_img_2010/1616301X-2010-20-9-ADFM200902043-content'> </P>

Chatter Analysis and Stability Prediction of Milling Tool Based on Zero-order and Envelope Methods for Real-time Monitoring and Compensation

Wen-Yang Chang,Chung-Cheng Chen,Sheng-Jhih Wu 한국정밀공학회 2019 International Journal of Precision Engineering and Vol.20 No.5

The artificial intelligence means that it can autonomously determine the cutting situations regardless any cutting states and change them automatically as required. Regenerative chatter is an instability occurrence during CNC machining operation that must be avoided for high accuracy and greater surface manufactures. In this paper, an artificial intelligence based on zero-order and enveloped method is use for the chatter analysis and stability prediction of milling tool in real-time and on-line compensations. In order to measure the phase shift of harmonic frequency for real-time in cutting process, two three-axis accelerometers are installed at the bottom of the workpiece and at the above of the spindle to collect the vibration signal. Experimental results showed that the phase shift of regenerative chatter is higher than unchartered. The stable chatter signals of time domain vibration according to stability lobe diagram have low amplitude of vibration. This was confirmed that characteristic marks of chatter vibrations have higher amplitude level signal in the experimental test. In addition, this study developed a chatter prediction system for on-line calculation and real-time monitoring and compensation. The modal parameters of the chatter analysis and stability prediction system like natural frequencies, damping, and residues must also be identified automatically.

Mechanical Error Analysis of Disk Cam Mechanisms with a Flat-Faced Follower

Chang Wen-Tung,Wu Long-Iong The Korean Society of Mechanical Engineers 2006 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.20 No.3

By employing the concept of equivalent linkage, this paper presents an analytical method for analyzing the mechanical errors of disk cam mechanisms with a flat-faced follower. The resulting error equations do not really involve the location of the curvature center of the cam profile, and locating the curvature center of the cam profile is not essential. The resulting errors are significantly affected by the pressure angle, and the smaller pressure angle will result in the smaller mechanical error. In the worst case, owing to the joined effects of various design parameters, the accuracy of the follower motion may degrade considerably. For the oscillating follower case, all acceleration error functions have a sudden change at every beginning and at every end of the motion even though the theoretical follower displacement is cycloidal motion.

Establishment of the Model Widely Valid for the Melting and Vaporization Zones in Selective Laser Melting Printings Via Experimental Verifications

Chang-Shuo Chang,Kuan-Ta Wu,Chang-Fu Han,Tsung-Wen Tsai,Sung-Ho Liu,Jen-Fin Lin 한국정밀공학회 2022 International Journal of Precision Engineering and Vol.9 No.1

The thermally affected material properties operating in the three phases and porosity variations in the SS316L steel powder have been introduced to the numerical analyses for the transient volumetric heat source (Q) models developed for the solid powder, melting, and vaporization regions in the selective laser melting (SLM). The bulk Q is thus a function of these heat sources and their ratio defined for the liquid and vapor phases. The heat conduction developed for the solid powders with porosity strings the heat convection with Q as the moving heat source to solve two-dimensional temperature distributions efficiently without the confinement of operating conditions and phase presumption. The specimens with single- and multiple-track printings are prepared to investigate the effects of incident energy density (E) and power intensity (I o) on the geometries of single-track printings and the areal surface roughness (Sa) values of the multiple-track printings with 0 and 50% overlap ratios. Laser power and scanning velocity are the controlling factors for the melting pool depth D and width W . D and W become the governing factors for the keyhole with evaporations, which affects the height H of single track after solidification. The W and D results predicted by the theoretical models developed in this study have an error range, 5–20%, compared to the experimental ones, which is much lower than those reported in the literatures (Gusarov et al. in J Heat Transf 131(7):072101, 2009. https ://doi.org/10.1115/1.31092 45 ; Hussein et al. in Mater Des 52:638–647, 2013. https ://doi.org/10.1016/j.matde s.2013.05.070 ; Yin et al. Int J Adv Manuf Technol 83(9–12): 1847–1859, 2016. https ://doi.org/10.1007/s0017 0-015-7609-x ; Andreotta et al. in Finite Elem Anal Des 135: 36–43, 2017. https ://doi.org/10.1016/j.finel .2017.07.002 ). The contact angle ( ϕ * ) is defined as a function of single-track width ( W ) and solidification height ( H ). ϕ * and Sa are significantly reduced as an E is applied beyond its critical value (47.62–57.14 J/mm 3 ). Significant change in Sa is ascribed to the big difference in the morphology and its surface pattern when E or I o reaches its critical value.

Applying Mobile Agent to a Mobile Stock Intermediary Services System Development

Wen-Hsiung Wu,Po-Chang Ko,Ping-Chen Lin,Ming-Hua Su 보안공학연구지원센터 2008 International Journal of Smart Home Vol.2 No.2

Due to the radical changing of the global economy, a more precise stock valuation helps providing important judgment principles to decision-makers and investors. With the advent of the third-generation (3G) or future forth-generation (4G) Internet, the mobile commerce (MCommerce) will become increasingly important. In addition, the mobile stock investment decision support system attracts great interests for professionals, such as stockholders, bondholders, financial analysts, governmental officials, and even the general public, recently. This study introduces a Mobile Agent-based Stock Intermediary Services System (MASISS) framework based on the mobile agent perspective to provide ubiquitous and seamless transaction activities for financial institutions. It also helps customers to make a more precise decision in the current intense commercial competition environment. For building distributed enterprise systems, The MASISS framework is developed in an integration of J2ME and J2EE environment with cross-platform portability, a huge server-side and client-side deployment base, and coverage for most W3C standards..

Comparison of Cortical Bone Trajectory to Pedicle-Based Dynamic Stabilization: An Analysis of 291 Patients

Chih-Chang Chang,Hsuan-Kan Chang,Chin-Chu Ko,Ching-Lan Wu,Yi-Hsuan Kuo,Tsung-Hsi Tu,Wen-Cheng Huang,Jau-Ching Wu 대한척추신경외과학회 2023 Neurospine Vol.20 No.1

Objective: Pedicle-based dynamic stabilization (DS) has gained popularity outside of America. Although pedicle screw (PS) loosening has always been a concern, it is reportedly innocuous. Cortical bone trajectory (CBT) screw is an emerging option with less invasiveness and similar effectiveness to PS in short-segment lumbar fusion. This study aimed to verify the use of CBT for DS by comparing the outcomes between pedicle- and CBT-based DS. Methods: Consecutive patients with lumbar spondylosis or low-grade spondylolisthesis who underwent 1- or 2-level DS between L3–5 with a minimum follow-up of 24 months were reviewed. Screw loosening was determined by computed tomography and the incidences were compared. Results: A total of 291 patients who underwent Dynesys DS (235 pedicle- and 56 CBT-based, respectively) were compared. The demographics and preoperative conditions were similar. All the clinical outcomes improved at 24-month postoperation, while the CBT-based group had less operation time and blood loss than the pedicle-based group. The rates of screw loosening were lower in the CBT-based (5.4% per screw and 12.5% per patient) than the pedicle-based group (9% per screw and 26.4% per patient). Furthermore, there were no differences in the clinical outcomes and complication profiles. Conclusion: The CBT-based DS for 1- or 2-level lumbar degeneration demonstrated equivalent clinical improvement as the pedicle-based DS. The adaption of CBT-based screws for DS could be a less invasive approach (shorter operation time and less blood loss), with lower chances of screw loosening than the conventional PS-based DS.

Measurement of Deformity at the Craniovertebral Junction: Correlation of Triangular Area and Myelopathy

Chih-Chang Chang,Jau-Ching Wu,Chin-Chu Ko,Hsuan-Kan Chang,Yi-Hsuan Kuo,Chao-Hung Kuo,Tsung-Hsi Tu,Wen-Cheng Huang 대한척추신경외과학회 2022 Neurospine Vol.19 No.4

Objective: Diseases of the craniovertebral junction (CVJ) are commonly associated with deformity, malalignment, and subsequent myelopathy. The misaligned CVJ might cause compression of neuronal tissues and subsequently clinical symptoms. The triangular area (TA), measured by magnetic resonance imaging/images (MRI/s), is a novel measurement for quantification of the severity of compression to the brain stem. This study aimed to assess the normal and pathological values of TA by a comparison of patients with CVJ disease to age- and sex-matched controls. Moreover, postoperative TAs were correlated with outcomes. Methods: Consecutive patients who underwent surgery for CVJ disease were included for comparison to an age- and sex-matched cohort of normal CVJ persons as controls. The demographics, perioperative information, and pre- and postoperative 2-year cervical MRIs were collected for analysis. Cervical TAs were measured and compared. Results: A total of 201 patients, all of whom had pre- or postoperative MRI, were analyzed. The TA of the CVJ deformity group was larger than the healthy control group (1.62 ± 0.57 cm2 vs. 1.01 ± 0.18 cm2 , p < 0.001). Moreover, patients who had combined anterior odontoidectomy and posterior laminectomy with fixation had the greatest reduction in the TA (1.18 ± 0.58 cm2 ). Conclusion: In CVJ deformity, the measurement of the cervical TA could indicate the severity of brain stem compression. After surgery, the TA had a varying degree of improvement, which could represent the efficacy of surgery.

The Option of Motion Preservation in Cervical Spondylosis: Cervical Disc Arthroplasty Update

Chih-Chang Chang,Wen-Cheng Huang,Jau-Ching Wu,Praveen V. Mummaneni 대한척추신경외과학회 2018 Neurospine Vol.15 No.4

Cervical disc arthroplasty (CDA), or total disc replacement, has emerged as an option in the past two decades for the management of 1- and 2-level cervical disc herniation and spondylosis causing radiculopathy, myelopathy, or both. Multiple prospective randomized controlled trials have demonstrated CDA to be as safe and effective as anterior cervical discectomy and fusion, which has been the standard of care for decades. Moreover, CDA successfully preserved segmental mobility in the majority of surgical levels for 5–10 years. Although CDA has been suggested to have long-term efficacy for the reduction of adjacent segment disease in some studies, more data are needed on this topic. Surgery for CDA is more demanding for decompression, because indirect decompression by placement of a tall bone graft is not possible in CDA. The artificial discs should be properly sized, centered, and installed to allow movement of the vertebrae, and are commonly 6 mm high or less in most patients. The key to successful CDA surgery includes strict patient selection, generous decompression of the neural elements, accurate sizing of the device, and appropriately centered implant placement.