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        Research of the Rice Image Segmentation Based on Color Linear Array CCD

        Weifeng Zhong,Yaji Xie,Xiaoxi He 보안공학연구지원센터 2014 International Journal of Control and Automation Vol.7 No.11

        Using color linear array CCD (Charge Coupled Device) to collect the information of image has an important meaning in the research of the machine vision processing. It now becomes the focus of study on CCD image acquisition using FPGA (Field Programmable Gate Array) to implement color linear array CCD-driven design. The image acquisition and processing of the rice color sorter as the research background in this paper, by using FPGA to achieve the color linear array CCD driven control, as well as simulated with Quartus II 8.0. In order to be able to extract the color linear array CCD output useful information of the color images, through study the RGB model and the HSI (Huge, Saturation, Intensity) model of color space model, the HSI space mode is selected as the color image processing space model, and design the suitable algorithm-FCM (Fuzzy C-Means) clustering algorithm in HSI space for color image segmentation, through a fuzzy Toolbox in MATLAB to accomplish the FCM algorithm, at the same time, through the simulation realized the FCM algorithm. The simulation shows that the algorithm can segment the image useful information well.

      • Study on SOC Estimation of Power Battery Based on Kalman Filter Optimization Algorithm

        Yuheng Yin,Weifeng Zhong 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.7

        Power battery's remaining capacity is affected by many interior and exterior uncertainties during use. To achieve estimate of current remaining measurable battery power by using battery measurable parameter data, which has always been the core issue of electric vehicle battery management system and the technical difficulties need to be resolved. In this paper, Kalman filtering theory is introduced in estimation algorithm, and then combined with open circuit voltage method and ampere measurement method, the SOC estimation according with the requirements of the electric car is achieved.

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        The effects of deletion of cellobiohydrolase genes on carbon source-dependent growth and enzymatic lignocellulose hydrolysis in Trichoderma reesei

        Ren Meibin,Wang Yifan,Liu Guoxin,Zuo Bin,Zhang Yuancheng,Wang Yunhe,Liu Weifeng,Liu Xiangmei,Zhong Yaohua 한국미생물학회 2020 The journal of microbiology Vol.58 No.8

        The saprophytic fungus Trichoderma reesei has long been used as a model to study microbial degradation of lignocellulosic biomass. The major cellulolytic enzymes of T. reesei are the cellobiohydrolases CBH1 and CBH2, which constitute more than 70% of total proteins secreted by the fungus. However, their physiological functions and effects on enzymatic hydrolysis of cellulose substrates are not sufficiently elucidated. Here, the cellobiohydrolase-encoding genes cbh1 and cbh2 were deleted, individually or combinatively, by using an auxotrophic marker-recycling technique in T. reesei. When cultured on media with different soluble carbon sources, all three deletion strains (Δcbh1, Δcbh2, and Δcbh1Δcbh2) exhibited no dramatic variation in morphological phenotypes, but their growth rates increased apparently when cultured on soluble cellulase-inducing carbon sources. In addition, Δcbh1 showed dramatically reduced growth and Δcbh1Δcbh2 could hardly grew on microcrystalline cellulose (MCC), whereas all strains grew equally on sodium carboxymethyl cellulose (CMC-Na), suggesting that the influence of the CBHs on growth was carbon source-dependent. Moreover, five representative cellulose substrates were used to analyse the influence of the absence of CBHs on saccharification efficiency. CBH1 deficiency significantly affected the enzymatic hydrolysis rates of various cellulose substrates, where acid pre-treated corn stover (PCS) was influenced the least. CBH2 deficiency reduced the hydrolysis of MCC, PCS, and acid pre-treated and delignified corncob but improved the hydrolysis ability of filter paper. These results demonstrate the specific contributions of CBHs to the hydrolysis of different types of biomass, which could facilitate the development of tailor-made strains with highly efficient hydrolysis enzymes for certain biomass types in the biofuel industry.

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