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- 저자 : Wark, Alastair W. (검색결과 16 건)
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PROPAGATION OF SURFACE WAVES ON IRREGULAR BED TOPOGRAPHY
WARKE, A. S.,DAS, S. K.,DEBNATH, L. 한국전산응용수학회 2006 Journal of applied mathematics & informatics Vol.20 No.1
This paper deals with the exact solution of surface gravity waves in an ocean with irregular bed topography. In order to obtain water surface elevation and run-up of infra-gravity waves when the bed is either wavy or exponential, closed form solutions are obtained. Numerical computations indicate that when solitary wave or sinusoidal wave conditions are applied at the boundary, water surface elevation attains near Gaussian profile.
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Bioaffinity detection of pathogens on surfaces
Wark, A.W.,Lee, J.,Kim, S.,Faisal, S.N.,Lee, H.J. Korean Society of Industrial and Engineering Chemi 2010 Journal of industrial and engineering chemistry Vol.16 No.2
The demand for improved technologies capable of rapidly detecting pathogens with high sensitivity and selectivity in complex environments continues to be a significant challenge that helps drive the development of new analytical techniques. Surface-based detection platforms are particularly attractive as multiple bioaffinity interactions between different targets and corresponding probe molecules can be monitored simultaneously in a single measurement. Furthermore, the possibilities for developing new signal transduction mechanisms alongside novel signal amplification strategies are much more varied. In this article, we describe some of the latest advances in the use of surface bioaffinity detection of pathogens. Three major sections will be discussed: (i) a brief overview on the choice of probe molecules such as antibodies, proteins and aptamers specific to pathogens and surface attachment chemistries to immobilize those probes onto various substrates, (ii) highlighting examples among the current generation of surface biosensors, and (iii) exploring emerging technologies that are highly promising and likely to form the basis of the next generation of pathogenic sensors.
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Bioaffinity detection of pathogens on surfaces
Alastair W. Wark,이재영,김수희,Shaikh Nayeem Faisal,이혜진 한국공업화학회 2010 Journal of Industrial and Engineering Chemistry Vol.16 No.2
The demand for improved technologies capable of rapidly detecting pathogens with high sensitivity and selectivity in complex environments continues to be a significant challenge that helps drive the development of new analytical techniques. Surface-based detection platforms are particularly attractive asmultiple bioaffinity interactions between different targets and corresponding probemolecules can be monitored simultaneously in a single measurement. Furthermore, the possibilities for developing new signal transduction mechanisms alongside novel signal amplification strategies aremuchmore varied. In this article, we describe some of the latest advances in the use of surface bioaffinity detection of pathogens. Three major sections will be discussed: (i) a brief overview on the choice of probe molecules such as antibodies, proteins and aptamers specific to pathogens and surface attachment chemistries to immobilize those probes onto various substrates, (ii) highlighting examples among the current generation of surface biosensors, and (iii) exploring emerging technologies that are highly promising and likely to form the basis of the next generation of pathogenic sensors.
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Sim, Hye Rim,Wark, Alastair W.,Lee, Hye Jin Royal Society of Chemistry 2010 The Analyst Vol.135 No.10
<P>This paper describes an ultrasensitive surface plasmon resonance (SPR) detection method using biofunctionalized gold nanorods for the direct detection of protein biomarkers. Immunoglobulin E (IgE), which has separate antibody and DNA aptamer binding sites, was chosen as a model protein for which a sandwich assay platform was designed. Detection was achieved <I>via</I> the specific adsorption of unlabelled IgE proteins onto the surface immobilized aptamer followed by the specific adsorption of anti-IgE coated gold nanorods (Au-NRs). Using the biofunctionalized nanorods in conjunction with SPR, we were able to directly measure IgE proteins at attomolar concentrations. This is a remarkable 10<SUP>8</SUP> enhancement compared to conventional SPR measurements of the same surface sandwich assay format ‘anti-IgE/IgE/surface bound IgE-aptamer’ in the absence of gold nanorod signal amplification.</P> <P>Graphic Abstract</P><P>A novel detection method utilizing antibody functionalized gold nanorods in conjunction with surface plasmon resonance was developed for the attomolar detection of protein biomarkers. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c0an00457j'> </P>
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Jang, Hye Ri,Wark, Alastair W.,Baek, Seung Hee,Chung, Bong Hyun,Lee, Hye Jin American Chemical Society 2014 ANALYTICAL CHEMISTRY - Vol.86 No.1
<P>One of the main challenges in the development of new analytical platforms for ultrasensitive bioaffinity detection is jointly achieving a wide dynamic range in target analyte concentration, especially for approaches that rely on multistep processes as a part of the signal amplification mechanism. In this paper, a new surface-based sandwich assay is introduced for the direct detection of B-type natriuretic peptide (BNP), an important biomarker for cardiac failure, at concentrations ranging from 1 aM to 500 nM. This was achieved using nanoparticle-enhanced surface plasmon resonance (SPR) where a DNA aptamer is immobilized on a chemically modified gold surface in conjunction with the specific adsorption of antiBNP coated gold nanocubes in the presence of the biomarker target. A concentration detection range greater than eleven orders of magnitude was achieved through dynamic control of only the secondary nanoparticle probe concentration. Furthermore, detection at low attomolar concentrations was also achieved in undiluted human serum.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancham/2014/ancham.2014.86.issue-1/ac4033565/production/images/medium/ac-2013-033565_0009.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ac4033565'>ACS Electronic Supporting Info</A></P>
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Baek, Seung Hee,Wark, Alastair W.,Lee, Hye Jin American Chemical Society 2014 ANALYTICAL CHEMISTRY - Vol.86 No.19
<P>A novel dual nanoparticle amplification approach is introduced for the enhanced surface plasmon resonance (SPR) detection of a target protein at subattomolar concentrations. Thrombin was used as a model target protein as part of a sandwich assay involving an antithrombin (anti-Th) modified SPR chip surface and a thrombin specific DNA aptamer (Th-aptamer) whose sequence also includes a polyadenine (A<SUB>30</SUB>) tail. Dual nanoparticle (NP) enhancement was achieved with the controlled hybridization adsorption of first polythymine-NP conjugates (T<SUB>20</SUB>-NPs) followed by polyadenine-NPs (A<SUB>30</SUB>-NPs). Two different nanoparticle shapes (nanorod and quasi-spherical) were explored resulting in four different NP pair combinations being directly compared. It was found that both the order and NP shape were important in optimizing the assay performance. The use of real-time SPR measurements to detect target concentrations as low as 0.1 aM is a 10-fold improvement compared to single NP-enhanced SPR detection methods.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancham/2014/ancham.2014.86.issue-19/ac5024183/production/images/medium/ac-2014-024183_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ac5024183'>ACS Electronic Supporting Info</A></P>
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Husain, Aliabbas A.,Daginawala, Hatim F.,Warke, Shubangi R.,Kalorey, Devanand R.,Kurkure, Nitin V.,Purohit, Hemant J.,Taori, Girdhar M.,Kashyap, Rajpal S. The Korean Association of Immunobiologists 2015 Immune Network Vol.15 No.2
Evaluation and screening of vaccines against tuberculosis depends on development of proper cost effective disease models along with identification of different immune markers that can be used as surrogate endpoints of protection in preclinical and clinical studies. The objective of the present study was therefore evaluation of subcutaneous model of M.tuberculosis infection along with investigation of different immune biomarkers of tuberculosis infection in BALB/c mice. Groups of mice were infected subcutaneously with two different doses : high ($2{\times}10^6CFU$) and low doses ($2{\times}10^2CFU$) of M.tuberculosis and immune markers including humoral and cellular markers were evaluated 30 days post M.tuberculosis infections. Based on results, we found that high dose of subcutaneous infection produced chronic disease with significant (p<0.001) production of immune markers of infection like $IFN{\gamma}$, heat shock antigens (65, 71) and antibody titres against panel of M.tuberculosis antigens (ESAT-6, CFP-10, Ag85B, 45kDa, GroES, Hsp-16) all of which correlated with high bacterial burden in lungs and spleen. To conclude high dose of subcutaneous infection produces chronic TB infection in mice and can be used as convenient alternative to aerosol models in resource limited settings. Moreover assessment of immune markers namely mycobacterial antigens and antibodies can provide us valuable insights on modulation of immune response post infection. However further investigations along with optimization of study protocols are needed to justify the outcome of present study and establish such markers as surrogate endpoints of vaccine protection in preclinical and clinical studies in future.
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Kwon, Min Jeong,Lee, Jaeyoung,Wark, Alastair W.,Lee, Hye Jin American ChemicalSociety 2012 ANALYTICAL CHEMISTRY - Vol.84 No.3
<P>The application of biofunctionalized nanoparticles possessing various shapes and sizes for the enhanced surface plasmon resonance (SPR) detection of a protein biomarker at attomolar concentrations is described. Three different gold nanopartide shapes (cubic cages, rods and quasi-spherical) with each possessing at least one dimension in the 40-50 nm range were systematically compared. Each nanoparticle (NP) was covalently functionalized with an antibody (anti-thrombin) and used as part of a sandwich assay in conjunction with a Au SPR chip modified with a DNA-aptamer probe specific to thrombin. The concentration of each NP-antibody conjugate solution was first optimized prior to establishing that the quasi-spherical nanopartides resulted in the greatest enhancement in sensitivity with the detection of thrombin at concentrations as low as 1 aM. When nanorod and nanocage antibody conjugates were instead used, the minimum target concentrations detected were 10 aM (rods) and 1 fM (cages). This is a significant improvement (>10(3)) on previous NP-enhanced SPR studies utilizing smaller (similar to 15 nm) gold NP conjugates and is attributed to the functionalization of both the NP and chip surfaces resulting in low nonspecific adsorption as well as a combination of density increases and plasmonic coupling inducing large shifts in the local refractive index at the chip surface upon nanoparticle adsorption.</P>
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