Changes in Protein Phosphorylation during Salivary Gland Degeneration in Haemaphysalis longicornis

Qi Xiao,Yuhong Hu,Xiaohong Yang,Jianna Tang,Xiaoshuang Wang,Xiaomin Xue,Mengxue Li,Minjing Wang,Yinan Zhao,Jingze Liu,Hui Wang 대한기생충학ㆍ열대의학회 2020 The Korean Journal of Parasitology Vol.58 No.2

The ticks feed large amount of blood from their hosts and transmit pathogens to the victims. The salivary gland plays an important role in the blood feeding. When the female ticks are near engorgement, the salivary gland gradually loses its functions and begins to rapidly degenerate. In this study, data-independent acquisition quantitative proteomics was used to study changes in the phosphorylation modification of proteins during salivary gland degeneration in Haemaphysalis longicornis. In this quantitative study, 400 phosphorylated proteins and 850 phosphorylation modification sites were identified. Trough RNA interference experiments, we found that among the proteins with changes in phosphorylation, apoptosis-promoting Hippo protein played a role in salivary gland degeneration.

Involvement of MicroRNA-198 Overexpression in the Poor Prognosis of Esophageal Cancer

Qi, Bo,Yao, Wen-Jian,Zhao, Bao-Sheng,Qin, Xiu-Guang,Wang, Yi,Wang, Wen-Ju,Wang, Tian-Yun,Liu, Shang-Guo,Li, Han-Chen Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.9

Objective: This study aimed to investigate whether the miR-198 expression level is related to clinicopathological factors and prognosis of esophageal cancer. Methods: MicroRNA was extracted from esophageal cancer patients who underwent surgery for assessment using the Taqman@ MicroRNA assay. The correlation between miR-198 expression and clinicopathological features was analyzed, and the significance of miR-198 as a prognostic factor and its relationship with survival was determined. Results: MicroRNA-198 (miR-198) expression was higher in patients with poor prognosis than those with good prognosis (P<0.05). Kaplan-Meier analysis results showed that the miR-198 expression level had a significant correlation with survival time (P=0.030) and that patients with a higher expression of miR-198 had a shorter survival time. Cox multi-factor model analysis showed that patient prognosis (P=0.014), tumor length (P=0.040) and expression (P=0.012), and survival time had a significant correlation; the corresponding risks were 7.268, 1.246, and 3.524, respectively. Conclusion: miR-198 overexpression is involved in the poor prognosis of esophageal cancer and can be used as a biomarker for selection of cases requiring especial attention.

Molecular characterization of chemosensory protein genes in Bactrocera minax (Diptera: Tephritidae)

Wang Zhao‐Xiang,Qi Zhen‐hua,Chen Jian,Wang Fu‐Lian,Gui Lian‐You,Zhang Guo‐Hui 한국곤충학회 2021 Entomological Research Vol.51 No.7

Chemoreception is of great importance for survival of insects. Chemosensory proteins (CSPs) are believed to be involved in the perireceptor events of chemosensory system in many insect species, but this has not been clarified in the citrus fruit fly, Bactrocera minax, a serious dipteran pest of citrus crops. Previous studies indicated that four CSP genes were identified in many fly speices in Diptera. In the present study, we also identified four CSP genes in B. minax,namely BminCSP1–4, from a transcriptome database. All CSP proteins encoded by these genes bear the typical hallmarks of the CSP family: an N-terminal signal peptide and the four highly conserved cysteine residues. Phylogenetic analysis comparing with other dipteran CSPs indicated that dipteran CSPs may evolved from three ancestral CSP genes, and revealed the sequence diversities of BminCSPs and showed that BminCSP1–4 are clustered in separate groups, indicating the possibility of their contrasting function in B. minax. Furthermore, the tissue distribution of the four CSP genes in adult B. minax was analyzed by real-time quantitiative PCR. The results demonstrated that BminCSP3 are significantly transcriptionally enriched in antennae; BminCSP4 expressed primarily in heads; BminCSP1 and BminCSP2 showed high expressions in different tissues, such as antennae, abdomens and wings. Based on these findings, the different implications for the functions of BminCSPs are discussed. This study will offer a significant indication for further functional studies of the CSPs in B. minax

Metabolic Pathways-based Two-step Selective Cluster Analysis: a New Method for Identification of Different Phenotypes of Breast Cancer

Zhao-qi Wang,Ping Chen,Hong Xia,Ping Zhou 보안공학연구지원센터 2013 International Journal of Bio-Science and Bio-Techn Vol.5 No.2

A review of experimental methods for characterising composite viscosities of continuous fibre-reinforced polymer composites

Wang Jinhuo,Han Yang,Ge Xiaohong,Qi Zhengbing,Zhao Jun,Wang Rongwen,Wu Huawei,Han Taiping,Sun Shaoxun,Wang Hui,Lin Jia,Liu Yuejun,Kong Xiangsong,Chen Qiming,Zeng Xiangxu 한국유변학회 2023 Korea-Australia rheology journal Vol.35 No.2

Optimisation design of composite structures requires an accurate predictive model for forming behaviour. The simulation process contains a number of model parameters which include transverse and longitudinal viscosities of continuous fibrereinforced viscous composites, fundamental to predicting the shear rheology. Shearing the unidirectional composite along the fibre direction gives a measure of the longitudinal viscosity (LV), whilst shearing across or transverse to the fibre direction gives a measure of the transverse viscosity (TV). Numerous experimental work was conducted in the past to measure these two viscosities for various materials. However, conflicting measurements by different test methods were obtained and these apparent discrepancies had not yet been systematically investigated in any single study. This paper reviews previous work on characterisation techniques to further understand the cause of such discrepancy, and hence to improve measurement accuracy, which would benefit future work on theoretical modelling of the composite viscosities and optimisation simulation of composites forming. Some important findings, such as effects of resin-rich areas, contributory factors of elastic effects, non-Newtonian behaviour for composites with Newtonian matrix, aspect ratio and end effects of test samples, geometry effects of fibres and fibre rearrangement during shearing, existence of a mathematical relationship between LV and TV and necessary benchmarking exercise using Newtonian matrix composites, were summarised.

Dissecting Causal Relationships Between Gut Microbiota, Blood Metabolites, and Stroke: A Mendelian Randomization Study

Wang Qi,Dai Huajie,Hou Tianzhichao,Hou Yanan,Wang Tiange,Lin Hong,Zhao Zhiyun,Li Mian,Zheng Ruizhi,Wang Shuangyuan,Lu Jieli,Xu Yu,Liu Ruixin,Ning Guang,Wang Weiqing,Bi Yufang,Zheng Jie,Xu Min 대한뇌졸중학회 2023 Journal of stroke Vol.25 No.3

Background and Purpose We investigated the causal relationships between the gut microbiota (GM), stroke, and potential metabolite mediators using Mendelian randomization (MR). Methods We leveraged the summary statistics of GM (n=18,340 in the MiBioGen consortium), blood metabolites (n=115,078 in the UK Biobank), and stroke (cases n=60,176 and controls n=1,310,725 in the Global Biobank Meta-Analysis Initiative) from the largest genome-wide association studies to date. We performed bidirectional MR analyses to explore the causal relationships between the GM and stroke, and two mediation analyses, two-step MR and multivariable MR, to discover potential mediating metabolites. Results Ten taxa were causally associated with stroke, and stroke led to changes in 27 taxa. In the two-step MR, <i>Bifidobacteriales</i> order, <i>Bifidobacteriaceae</i> family, <i>Desulfovibrio</i> genus, apolipoprotein A1 (ApoA1), phospholipids in high-density lipoprotein (HDL_PL), and the ratio of apolipoprotein B to ApoA1 (ApoB/ApoA1) were causally associated with stroke (all <i>P</i><0.044). The causal associations between <i>Bifidobacteriales</i> order, <i>Bifidobacteriaceae</i> family and stroke were validated using the weighted median method in an independent cohort. The three GM taxa were all positively associated with ApoA1 and HDL_PL, whereas <i>Desulfovibrio</i> genus was negatively associated with ApoB/ApoA1 (all <i>P</i><0.010). Additionally, the causal associations between the three GM taxa and ApoA1 remained significant after correcting for the false discovery rate (all q-values <0.027). Multivariable MR showed that the associations between <i>Bifidobacteriales</i> order, <i>Bifidobacteriaceae</i> family and stroke were mediated by ApoA1 and HDL_PL, each accounting for 6.5% (<i>P</i>=0.028) and 4.6% (<i>P</i>=0.033); the association between <i>Desulfovibrio</i> genus and stroke was mediated by ApoA1, HDL_PL, and ApoB/ApoA1, with mediated proportions of 7.6% (<i>P</i>=0.019), 4.2% (<i>P</i>=0.035), and 9.1% (<i>P</i>=0.013), respectively. Conclusion The current MR study provides evidence supporting the causal relationships between several specific GM taxa and stroke and potential mediating metabolites.

Study on Hydration and Hardening Performance of Coal Gangue-Steel Slag-Cement Composite Cementitious Material

Xiaozhi Zhao,Liang Wang,Chenglong Wang,Jian Xu,Wei Hu,Qi Li,Hao Wang 대한토목학회 2024 KSCE Journal of Civil Engineering Vol.28 No.5

In this paper, coal gangue powder (CGP) was used to replace 30% of the cement weight. The dosage of steel slag powder (SSP) was 5%, 10%, 15%, 20%, and 25% of the coal gangue weight. The CGP-SSP-cement mortars and pastes were prepared. The compressive and flexural strengths, hydration heat, X-ray diffraction (XRD), thermogravimetric analysis (TGA-DTG), pore structure (MIP), electric flux, and capillary water absorption were examined. Results displayed that the detrimental effects of single-doped CGP were reduced by adding SSP to a single 30% calcined coal gangue powder (CCGP). Compared with the single-doped 30% SSP, the compressive strength value prepared by 10% CCGP and 20% SSP at 28 days was 15.21% higher. Compared with the single-doped 30% SSP, the flexural strength value prepared by 15% CCGP and 15% SSP at 28 days was 15.78% higher. When 15% CCGP and 15% SSP were combined, the mortar's electric flux was 31.71% lower and the capillary water absorption was 9.48% lower compared with the single-doped 30% SSP. The microscopic results showed more hydration products such as AFt and CH, were produced under the synergistic effect of CCGP and SSP. The number of macropores was reduced compared with CCGP or SSP alone.

Surface Modification of Flax Fibers with Isocyanate and Its Effects on Fiber/Epoxy Interfacial Properties

Wanru Wang,Rao Fu,Qi Deng,Xinyuan Wang,Yujie Wang,Zhao Zhang,Guijun Xian 한국섬유공학회 2020 Fibers and polymers Vol.21 No.12

Natural fiber reinforced polymer composite has been widely used in various industry fields. Natural fibertreatment can effectively improve its mechanical and durability properties, and expands its applications. In the present study,isocyanate was proposed to treat flax fibers in order to reduce the hydrophilic properties of the fibers, and to enhance thebonding of flax fiber to epoxy matrix. The isocyanate treated fabric was evaluated with fourier transform infra-red (FTIR),scanning electron microscopy (SEM) and water uptake. The effects of the fiber treatment on the mechanical properties of flaxfabric reinforced epoxy (FFRP) plates were investigated. FTIR and SEM analysis indicated that isocyanate reacts with flaxfiber, forming a thin polymer layer on the fiber surface. The water absorption test showed that isocyanate treatment decreasedthe water uptake of the flax fabric by 4-18 times compared to the control fabric. The flexural and tensile strength of FFRPwith isocyanate solution treated fabrics were enhanced more than 20 %, attributed to the improved adhesion of fiber to epoxy. Based on the above results, the treatment method of flax fiber with isocyanate is considered as an effective approach toimprove both hydrothermal ageing resistance and mechanical properties of FFRPs.

ACTIVE LQR MULTI-AXLE-STEERING METHOD FOR IMPROVING MANEUVERABILITY AND STABILITY OF MULTI-TRAILER ARTICULATED HEAVY VEHICLES

You-Qun Zhao,Zhao Wen Deng,Qi Xian Zhao,Bao Hua Wang,Wei Gao,Xin Xin Kong 한국자동차공학회 2022 International journal of automotive technology Vol.23 No.4

Directional performance and highway stability are two important aspects that need to be considered in development and design of a heavy articulated vehicles. To improve the maneuverability and stability of a multi-trailer articulated heavy vehicle (MTAHV), an active linear quadratic regulator (LQR) multi-axle-steering method is designed and examined. First, a linear yaw-plane model with four-degree-of freedom (4-DOF) for MTAHV is built and validated. Thus, a reference model supplying the desired state responses is introduced. Then, an active control algorithm of multi-axle-steering for the rear axles of tractor and full-trailer is investigated, and a LQR controller is proposed based on the linear vehicle model to make the control variables track the desired state responses. The control strategy concentrates on keeping the actual yaw rate and side-slip angle follow the steady-state yaw rate and zero side-slip angle. Finally, the effectiveness of the designed approach on enhancing the maneuverability and stability of the MTAHV have been validated through the simulations of the low-speed 360o roundabout and the single lane-change maneuver with high speed, respectively. The method has a certain reference value for improving the active safety of the MTAHV.

Highly Efficient Biotransformation of Astragaloside IV to Cycloastragenol by Sugar-Stimulated β-Glucosidase and β-Xylosidase from Dictyoglomus thermophilum

( Qi Li ),( Tao Wu ),( Linguo Zhao ),( Jianjun Pei ),( Zhenzhong Wang ),( Wei Xiao ) 한국미생물 · 생명공학회 2019 Journal of microbiology and biotechnology Vol.29 No.12

β-Glucosidases and β-xylosidases are two categories of enzymes that could cleave out nonreducing, terminal β-D-glucosyl and β-D-xylosyl residues with release of D-glucose and Dxylose, respectively. In this paper, two functional β-glucosidase Dth3 and β-xylosidase Xln-DT from Dictyoglomus thermophilum were heterologously expressed in E.coli BL21 (DE3). Dth3 and Xln-DT were relatively stable at 75oC and were tolerant or even stimulated by glucose and xylose. Dth3 was highly tolerant to glucose with a Ki value of approximately 3 M. Meanwhile, it was not affected by xylose in high concentration. The activity of Xln-DT was stimulated 2.13- fold by 1 M glucose and 1.29-fold by 0.3 M xylose, respectively. Furthermore, the β- glucosidase Dth3 and β-xylosidase Xln-DT showed excellent selectivity to cleave the outer C-6 and C-3 sugar moieties of ASI, which established an effective and green method to produce the more pharmacologically active CAG, an exclusive telomerase activator. We measured temperature, pH and dosage of enzyme using a single-factor experiment in ASI biotransformation. After optimization, the optimal reaction conditions were as follows: 75oC, pH 5.5, 1 U of Dth3 and 0.2 U of Xln-DT, respectively. Under the optimized conditions, 1 g/l ASI was transformed into 0.63 g/l CAG with a corresponding molar conversion of 94.5% within 3 h. This is the first report to use the purified thermostable and sugar-tolerant enzymes from Dictyoglomus thermophilum to hydrolyze ASI synergistically, which provides a specific, environment-friendly and cost-effective way to produce CAG.