Kojic Acid Protects C57BL/6 Mice from Gamma-irradiation Induced Damage

Wang, Kai,Liu, Chao,Di, Chan-Juan,Ma, Cong,Han, Chun-Guang,Yuan, Mei-Ru,Li, Peng-Fei,Li, Lu,Liu, Yong-Xue Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.1

The radioprotective effects of a single administration of kojic acid (KA) against ionizing radiation were evaluated via assessment of 30-day survival and alterations of peripheral blood parameters of adult C57BL/6 male mice. The 30-day survival rate of mice pretreated with KA (75 or 300 mg/kg body weight, KA75 or KA300) subcutaneously 27 h prior to a lethal dose (8 Gy, 153.52 cGy/min) of gamma irradiation was higher than that of mice irradiated alone (40% or 60% vs 0%). It was observed that the white blood cell (WBC) count/the red blood cell (RBC) count, haemoglobin content, haematocrit and platelet count of mice with or without KA pretreatment as exposed to a sub-lethal dose (4 Gy, 148.14 cGy/min) of gamma irradiation decreased maximally at day 4/day 8 post-irradiation. Although the initial WBC values were low in KA300 or WR-2721 (amifostine) groups, they significantly recovered to normal at day 19, whereas in the control group they did not. The results from the cytotoxicity and cell viability assays demonstrated that KA could highly protect Chinese hamster ovary (CHO) cells against ionizing radiation with low toxicity. In summary, KA provides marked radioprotective effects both in vivo and in vitro.

RNA sequencing reveals that Prx II gene knockout can down-regulate the allograft rejection of dermal mesenchymal stem cells

Han Ying-Hao,Mao Ying-Ying,Yu Nan-Nan,Jin Mei-Hua,Jin Ying-Hua,Wang Ai-Guo,Zhang Yong-Qing,Shen Gui-Nan,Cui Yu-Dong,Yu Li-Yun,Lee Dong-Seok,Jo Yu-Jin,Sun Hu-Nan,Kwon Jeongwoo,권태호 한국응용생명화학회 2020 Applied Biological Chemistry (Appl Biol Chem) Vol.63 No.3

In this study, we used RNA sequencing (RNA-seq) to analyze and compare bulk cell samples from wild-type (WT) dermal mesenchymal stem cells (DMSCs) (n = 3) and Prx II knockout DMSCs (n = 3). The purpose of the study was to elucidate the role of Prx II on allogeneic immune rejection of transplanted DMSCs. The results revealed differential expression of 472 genes (176 up-regulated and 296 down-regulated; p ≤ 0.05) between the PrxII+/+ (WT) and PrxII−/− sample groups. When highly regulated genes were categorized according to the Gene Ontology (GO) molecular function classification and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, the PrxII−/− samples showed a robust downward trend in allograft rejection. The study identified 43 all immunologically rejected differentially expressed genes, of which 41 showed lower expression in the PrxII−/− vs. PrxII+/+ (WT) samples. These findings suggest that Prx II gene knockout may down-regulate the allograft rejection that occurs during DMSCs transplantation and improve the survival rate of DMSCs in the host. This study provides a new perspective on the clinical treatment of stem cell transplantation.

A Single-Phase Current-Source Bidirectional Converter for V2G Applications

Han, Hua,Liu, Yonglu,Sun, Yao,Wang, Hui,Su, Mei The Korean Institute of Power Electronics 2014 JOURNAL OF POWER ELECTRONICS Vol.14 No.3

In this paper, a single-phase current-source bidirectional converter topology for V2G applications is proposed. The proposed converter consists of a single-phase current-source rectifier (SCSR) and an auxiliary switching network (ASN). It offers bidirectional power flow between the battery and the grid in the buck or boost mode and expands the output voltage range, so that it can be compatible with different voltage levels. The topology structure and operating principles of the proposed converter are analyzed in detail. An indirect control algorithm is used to realize the charging and discharging of the battery. Finally, the semiconductor losses and system efficiency are analyzed. Simulation and experimental results demonstrate the validity and effectiveness of the proposed topology.

Negative Impact of Adjacent Coordination on Direct Electrochemistry and Enzymatic Catalysis of Laccase Immobilization onto Multi-wall Carbon Nanotubes Functionalized by Perylene Derivative

Han Zeng,Wen Shan Huo,Fang Wang,Ting Mei Ma 한국고분자학회 2019 Macromolecular Research Vol.27 No.10

Influence of interactions between Laccase and carbon nano-tubes modified by perylene derivative on structural parameters and electrochemical behaviors of immobilized redox protein, was investigated systematically by the means of spectrometry, photography and electro-chemistry. Sophisticated interactions including abutting coordination could result in anomalistic agglomeration of nano-composite with Lac incorporation which displayed better hydrophobicity than free Laccase and high resistance of charge shuttle. It would retard the progress of thermal decomposition of nano-composite and would lead to the formation of composite without emission of fluorescence. Strong binding of Laccase with perylenyl derivative (binding constant: 7.5×104 L/mol) would alter the mechanism of electron transferring. T1 Cu2+ of Laccase in the status of complexation played the intra-molecular electron relay with mid-wave potential at -135.6 mV and demonstrated undesirable dynamics in electron transferring (7.7×10-2 s-1). The catalytic function in oxygen reduction was prohibited for the abutting coordination and only weak catalytic effect could be achieved in the presence of electron relay. The whole catalytic cycle was limited by the mass transferring of electron mediator.

siRNAs Derived from Cymbidium Mosaic Virus and Odontoglossum Ringspot Virus Down-modulated the Expression Levels of Endogenous Genes in Phalaenopsis equestris

Han-hong Lan,Cui-mei Wang,Shuang-shuang Chen,Jian-ying Zheng 한국식물병리학회 2019 Plant Pathology Journal Vol.35 No.5

Interplay between Cymbidium mosaic virus (CymMV)/ Odontoglossum ringspot virus (ORSV) and its host plant Phalaenopsis equestris remain largely unknown, which led to deficiency of effective measures to control disease of P. equestris caused by infecting viruses. In this study, for the first time, we characterized viral small interfering RNAs (vsiRNAs) profiles in P. equestris co-infected with CymMV and ORSV through small RNA sequencing technology. CymMV and ORSV small interfering RNAs (siRNAs) demonstrated several general and specific/new characteristics. vsiRNAs, with A/ U bias at the first nucleotide, were predominantly 21-nt long and they were derived predominantly (90%) from viral positive-strand RNA. 21-nt siRNA duplexes with 0-nt overhangs were the most abundant 21-nt duplexes, followed by 2-nt overhangs and then 1-nt overhangs 21-nt duplexes in infected P. equestris. Continuous but heterogeneous distribution and secondary structures prediction implied that vsiRNAs originate predominantly by direct Dicer-like enzymes cleavage of imperfect duplexes in the most folded regions of the positive strand of both viruses RNA molecular. Furthermore, we totally predicted 54 target genes by vsiRNAs with psRNATarget server, including disease/stress response– related genes, RNA interference core components, cytoskeleton- related genes, photosynthesis or energy supply related genes. Gene Ontology classification showed that a majority of the predicted targets were related to cellular components and cellular processes and performed a certain function. All target genes were down-regulated with different degree by vsiRNAs as shown by realtime reverse transcription polymerase chain reaction. Taken together, CymMV and ORSV siRNAs played important roles in interplay with P. equestris by down modulating the expression levels of endogenous genes in host plant.

Effects of Selenium, Copper and Magnesium on Antioxidant Enzymes and Lipid Peroxidation in Bovine Fluorosis

Han, Bo,Yoon, Soonseek,Su, Jingliang,Han, H.R.,Wang, Mei,Qu, Weijie,Zhong, Daibin Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.12

The antioxidant enzymes, lipid peroxidation and free radicals assessment were made of the effects of selenium, copper and magnesium on bovine endemic fluorosis under high fluoride, low selenium and low copper productive conditions. Thirty-two beef cattle were selected from high fluoride area, and randomly divided into four groups with eight cattle each as follows: (1) high fluoride control group (HFC); (2) supplemented group with 0.25 mg/kg selenium (HFSe); (3) supplemented group with 15 mg/kg copper (HFCu) and (4) supplemented group with 0.25 mg/kg selenium+15 mg/kg copper+1 mg/kg magnesium (HFSeCuMg) per day for 83 days. Moreover, eight beef cattle were selected from non-high fluoride area as normal control group. Blood samples were collected from cattle on 0 d, 30 d and 83 d respectively, to analyze the enzyme activities and concentration of GSH-px, CAT, SOD, MDA and free radicals. The results showed that the contents of free radicals and MDA in HFC group were significantly higher, and the whole blood GSH-px, CAT, erythrocyte SOD activities were lower than the normal control group. Free radicals, metabolic imbalance and antioxidant disorder therefore, play an important role in fluorosis. However, GSH-px, CAT and SOD activities in HFSe group and HFSeCuMg group at 30 d and 83 d were markedly higher than the same groups at the 0 d and the HFC group at the same time. Likewise, there was a corresponding reduction in the contents of free radicals and MDA. These findings indicated that supplementation with selenium, copper and magnesium elevated high fluoride bovine antioxidant enzymes, and decreased MDA and free radicals contents. But, the activities of supplementation selenium group did not increase until day 83. These results demonstrated that fluorosis was associated with lower serum Se and Cu levels than in the control, and it was therefore concluded that fluorosis is associated with decreased serum levels of these minerals. Long-term high fluoride intake under productive condition enhances oxidative stress in the blood, thereby disturbing the antioxidant defense of cattle. Increased oxidative stress could be one of the mediating factors in the pathogenesis of toxic manifestations of fluoride. It is benefical for high fluoride cattle supplemented with proper selenium, copper and magnesium to increase fluoride excretion and obtain the protective impact of the activity of oxidative enzymes, and to decrease lipid peroxidation and free radicals contents.

Effect of Micronutrient Supplementation on the Growth of Preschool Children in China

Han, Junhua,Yang, Yuexin,Shao, Xiaoping,He, Mei,Bian, Lihua,Wang, Zhu The Korean Nutrition Society 2002 Nutritional Sciences Vol.5 No.3

The purpose of this study was to investigate the effects of micronutrient supplementation on the growth of preschool children in China. A double-blind, placebo-controlled trial was conducted on 156 growth retarded preschool children who were randomly assigned to the following five groups : supplemental control (S-control; n=28); zinc supplementation (+Zn; 3.5mg Zn/day, n=34); zinc and calcium supplementation (+ZnCa; 3.5mg Zn + 250mg Ca/day, n=37); zinc, calcium and vitamin A supplementation (+ZnCaVA; 3.5mgZn + 250mgCa + 200gVA/day, n=28); and calcium and vitamin A supplementation (+CaVA; 250mgCa + 200gVA/day, n=29). Another 34 children of normal height were selected as a normal control (N-control). Supplementation continued for twelve months. After supplementation, the height gains in the +Zn group (7.84cm per year) and the +ZnCa group (7.70 cm per year) were significantly higher than that in the S-control group (6.74 cm per year, P<0.05). The weight gain in the +ZnCaVA group (2.55kg per year) and the +CaVA group (2.57 kg per year) was also significantly higher than that in the S-control group (2.19 kg per year, P<0.05). The average number of days of illness in each group taking supplements was lower than that in the S-control group (13 days per year compared with 23 days per year). No significant differences in bone maturity were observed between the groups. In conclusion, in this study Zinc and Zinc + Calcium supplementation improved the height gain, and vitamin A improved the weight gain, in growth retarded preschool children, but these supplements did not affect the maturity of bone. Micronutrient supplementation also lowered the morbidity of these children.

Adaptive State-of-Charge Estimation Method for an Aeronautical Lithium-ion Battery Pack Based on a Reduced Particle-unscented Kalman Filter

Wang, Shun-Li,Yu, Chun-Mei,Fernandez, Carlos,Chen, Ming-Jie,Li, Gui-Lin,Liu, Xiao-Han The Korean Institute of Power Electronics 2018 JOURNAL OF POWER ELECTRONICS Vol.18 No.4

A reduced particle-unscented Kalman filter estimation method, along with a splice-equivalent circuit model, is proposed for the state-of-charge estimation of an aeronautical lithium-ion battery pack. The linearization treatment is not required in this method and only a few sigma data points are used, which reduce the computational requirement of state-of-charge estimation. This method also improves the estimation covariance properties by introducing the equilibrium parameter state of balance for the aeronautical lithium-ion battery pack. In addition, the estimation performance is validated by the experimental results. The proposed state-of-charge estimation method exhibits a root-mean-square error value of 1.42% and a mean error value of 4.96%. This method is insensitive to the parameter variation of the splice-equivalent circuit model, and thus, it plays an important role in the popularization and application of the aeronautical lithium-ion battery pack.

Design, synthesis and biological evaluation of B-region modified diarylalkyl amide analogues as novel TRPV1 antagonists

Han, Young Taek,Yang, Shao-Mei,Wang, Xiao-Yuan,Li, Fu-Nan 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.4

Design, synthesis and biological evaluation of B-region, known to be a dipolar interacting pharmacophore, modified diarylalkyl amide analogues for novel TRPV1 (transient receptor potential channel, vanilloid subfamily member 1) antagonists was described. A variety of moieties including guanidines, heterocyclic rings, cinnamides, and ${\alpha}$-substituted acetamides were introduced at the B-region. TRPV1 antagonistic activities of these analogues were evaluated by $^{45}Ca^{2+}$ uptake assay in rat DRG neuron. In particular, ${\alpha},{\alpha}$-difluoroamide 53 exhibited 3-fold more potent TRPV1 antagonistic activity ($IC_{50}=0.058{\mu}M$) than the parent amide analogue 6.

siRNAs Derived from Cymbidium Mosaic Virus and Odontoglossum Ringspot Virus Down-modulated the Expression Levels of Endogenous Genes in Phalaenopsis equestris

Lan, Han-hong,Wang, Cui-mei,Chen, Shuang-shuang,Zheng, Jian-ying The Korean Society of Plant Pathology 2019 Plant Pathology Journal Vol.35 No.5

Interplay between Cymbidium mosaic virus (CymMV)/Odontoglossum ringspot virus (ORSV) and its host plant Phalaenopsis equestris remain largely unknown, which led to deficiency of effective measures to control disease of P. equestris caused by infecting viruses. In this study, for the first time, we characterized viral small interfering RNAs (vsiRNAs) profiles in P. equestris co-infected with CymMV and ORSV through small RNA sequencing technology. CymMV and ORSV small interfering RNAs (siRNAs) demonstrated several general and specific/new characteristics. vsiRNAs, with A/U bias at the first nucleotide, were predominantly 21-nt long and they were derived predominantly (90%) from viral positive-strand RNA. 21-nt siRNA duplexes with 0-nt overhangs were the most abundant 21-nt duplexes, followed by 2-nt overhangs and then 1-nt overhangs 21-nt duplexes in infected P. equestris. Continuous but heterogeneous distribution and secondary structures prediction implied that vsiRNAs originate predominantly by direct Dicer-like enzymes cleavage of imperfect duplexes in the most folded regions of the positive strand of both viruses RNA molecular. Furthermore, we totally predicted 54 target genes by vsiRNAs with psRNATarget server, including disease/stress response-related genes, RNA interference core components, cytoskeleton-related genes, photosynthesis or energy supply related genes. Gene Ontology classification showed that a majority of the predicted targets were related to cellular components and cellular processes and performed a certain function. All target genes were down-regulated with different degree by vsiRNAs as shown by real-time reverse transcription polymerase chain reaction. Taken together, CymMV and ORSV siRNAs played important roles in interplay with P. equestris by down modulating the expression levels of endogenous genes in host plant.