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Han Zeng,Wen Shan Huo,Fang Wang,Ting Mei Ma 한국고분자학회 2019 Macromolecular Research Vol.27 No.10
Influence of interactions between Laccase and carbon nano-tubes modified by perylene derivative on structural parameters and electrochemical behaviors of immobilized redox protein, was investigated systematically by the means of spectrometry, photography and electro-chemistry. Sophisticated interactions including abutting coordination could result in anomalistic agglomeration of nano-composite with Lac incorporation which displayed better hydrophobicity than free Laccase and high resistance of charge shuttle. It would retard the progress of thermal decomposition of nano-composite and would lead to the formation of composite without emission of fluorescence. Strong binding of Laccase with perylenyl derivative (binding constant: 7.5×104 L/mol) would alter the mechanism of electron transferring. T1 Cu2+ of Laccase in the status of complexation played the intra-molecular electron relay with mid-wave potential at -135.6 mV and demonstrated undesirable dynamics in electron transferring (7.7×10-2 s-1). The catalytic function in oxygen reduction was prohibited for the abutting coordination and only weak catalytic effect could be achieved in the presence of electron relay. The whole catalytic cycle was limited by the mass transferring of electron mediator.
Adenoviral Vector Mediates High Expression Levels of Human Lactoferrin in the Milk of Rabbits
( Zeng Sheng Han ),( Qing Wang Li ),( Zhi Ying Zhang ),( Yong Sheng Yu ),( Bo Xiao ),( Shu Yun Wu ),( Zhong Liang Jiang ),( Hong Wei Zhao ),( Rui Zhao ),( Jian Li ) 한국미생물 · 생명공학회 2008 Journal of microbiology and biotechnology Vol.18 No.1
The limitations in current technology for generating transgenic animals, such as the time and the expense, hampered its extensive use in recombinant protein production for therapeutic purpose. In this report, we present a simple and less expensive alternative by directly infusing a recombinant adenovirus vector carrying human lactoferrin cDNA into rabbit mammary glands. The milk serum was collected from the infected mammary gland 48 h post-infection and subjected to a 10% SDS-PAGE and Western blotting. An 80-kDa protein was visualized after viral vector infection. With this method, we obtained a high level of expressed human lactoferrin of up to 2.3 mg/ml in the milk. Taken together, the method is useful for the transient high-level expression recombinant proteins, and the approach established here is probably one of the most economical and efficient ways for large-scale production of recombinant proteins of biopharmaceutical interest.
Cheng Han Ng,Kai En Chan,Yip Han Chin,Rebecca Wenling Zeng,Pei Chen Tsai,Wen Hui Lim,Darren Jun Hao Tan,Chin Meng Khoo,Lay Hoon Goh,Zheng Jye Ling,Anand Kulkarni,Lung-Yi Loey Mak,Daniel Q Huang,Mark C 대한간학회 2022 Clinical and Molecular Hepatology(대한간학회지) Vol.28 No.3
Background/Aims: Nonalcoholic fatty liver disease (NAFLD) is closely associated with diabetes. The cumulative impact of both diseases synergistically increases risk of adverse events. However, present population analysis is predominantly conducted with reference to non-NAFLD individuals and has not yet examined the impact of prediabetes. Hence, we sought to conduct a retrospective analysis on the impact of diabetic status in NAFLD patients, referencing non-diabetic NAFLD individuals. Methods: Data from the National Health and Nutrition Examination Survey 1999–2018 was used. Hepatic steatosis was defined with United States Fatty Liver Index (US-FLI) and FLI at a cut-off of 30 and 60 respectively, in absence of substantial alcohol use. A multivariate generalized linear model was used for risk ratios of binary outcomes while survival analysis was conducted with Cox regression and Fine Gray model for competing risk. Results: Of 32,234 patients, 28.92% were identified to have NAFLD. 36.04%, 38.32% and 25.63% were non-diabetic, prediabetic and diabetic respectively. Diabetic NAFLD significantly increased risk of cardiovascular disease (CVD), stroke, chronic kidney disease, all-cause and CVD mortality compared to non-diabetic NAFLD. However, prediabetic NAFLD only significantly increased the risk of CVD and did not result in a higher risk of mortality. Conclusions: Given the increased risk of adverse outcomes, this study highlights the importance of regular diabetes screening in NAFLD and adoption of prompt lifestyle modifications to reduce disease progression. Facing high cardiovascular burden, prediabetic and diabetic NAFLD individuals can benefit from early cardiovascular referrals to reduce risk of CVD events and mortality.
Yanling Zeng,Han Liu,Tianhui Zhu,Shan Han,Shujiang Li 한국식물병리학회 2021 Plant Pathology Journal Vol.37 No.3
Brown leaf spot disease caused by Nigrospora guilin- ensis on Phellodendron chinense occurs in a large area in Dayi County, Chengdu City, Sichuan Province, China each year. This outbreak has severely reduced the production of Chinese medicinal plants P. chinense and caused substantial economic losses. The bacterial isolate JKB05 was isolated from the healthy leaves of P. chinense, exhibited antagonistic effects against N. guilinensis and was identified as Bacillus megaterium. The following fermentation medium and conditions improved the inhibitory effect of B. megaterium JKB05 on N. guilinensis: 2% glucose, 0.1% soybean powder, 0.1% KCl, and 0.05% MgSO4; initial concentration 6 × 106 cfu/ml, and a 42-h optimal fermentation time. A composite of 0.1% nano-SiO2 JKB05 improved the thermal stability, acid-base stability and ultraviolet resistance by 16%, 12%, and 38.9%, respectively, and nano-SiO2 was added to the fermentation process. The best formula for the wettable powder was 35% kaolin, 4% polyethylene glycol, 8% Tween, and 2% humic acid. The following quality test results for the wettable powder were obtained: wetting time 87.0 s, suspension rate 80.33%, frequency of microbial contamination 0.08%, pH 7.2, fineness 95.8%, drying loss 1.47%, and storage stability ≥83.5%. A pot experiment revealed that the ability of JKB05 to prevent fungal infections on P. chinense increased considerably and achieved levels of control as high as 94%. The use of nanomaterials significantly improved the ability of biocontrol bacteria to control this disease.
Han, D.M.,Guo, Z.P.,Zeng, R.,Kim, C.J.,Meng, Y.Z.,Liu, H.K. Pergamon Press ; Elsevier Science Ltd 2009 International journal of hydrogen energy Vol.34 No.5
Carbon nanotube (CNT) containing electrocatalysts, Pt/Sn/PMo<SUB>12</SUB>/CNT, Pt/Sn/CNT, and Pt/CNT, were prepared by a microwave-heated polyol process. The electrocatalysts were characterized by X-ray diffraction, energy dispersive spectroscopy, scanning and transmission electron microscopy, and cyclic voltammetry. We found that the addition of PMo<SUB>12</SUB> can help Pt-Sn nanoparticles to disperse very uniformly on the outer walls of CNTs. The Pt/Sn/PMo<SUB>12</SUB>/CNT catalyst exhibited the lowest onset potential for electro-oxidation of adsorbed C intermediates, compared to the Pt/Sn/CNT and Pt/CNT catalysts. It also generated much higher current density for methanol oxidation at room temperature compared to Pt/Sn/CNT and Pt/CNT catalysts, which were prepared by the same method.
Zeng, Ri-Zhong,Kim, Han-Geun,Kim, Na-Ra,Lee, Hae-Young,Jung, Bong-Jun,Ko, Mi-Yeon,Lee, Seung-Yeon,Chung, Dae-Kyun Korean Society for Molecular and Cellular Biology 2010 Molecules and cells Vol.29 No.6
Lipoteichoic acid (LTA) from Staphylococcus aureus (aLTA) and from Lactobacillus plantarum LTA (pLTA) are both recognized by Toll-like receptor 2 (TLR2), but cause different stimulatory effects on the innate immune and inflammatory responses, and their underlying cellular mechanisms are unknown. In this study, comparative proteome analysis was performed using two-dimensional gel electrophoresis and mass spectrometry on protein extracts from human monocyte THP-1 cells stimulated with either aLTA or pLTA. Differentially expressed proteins might be involved in innate immunity and inflammation. Cells treated with aLTA and with pLTA showed different protein expression profiles. Of 60 identified proteins, 10 were present only in treated cells (8 in aLTA-treated only, and 2 in pLTA-treated only), 1 protein (IMPDH2) was suppressed by pLTA, and 49 were up- or down-regulated more than three-fold by aLTA- or pLTA- stimulation. Several proteins involved in immunity or inflammation, anti-oxidation, or RNA processing were significantly changed in expression by aLTA- or pLTA-stimulation, including cyclophilin A, HLA-B27, D-dopachrome tautomerase, Mn-SOD, hnRNP-C, PSF and KSRP. These data demonstrated that aLTA and pLTA had different effects on the protein profile of THP-1 cells. Comparison of the proteome alterations will provide candidate biomarkers for further investigation of the immunomodulatory effects of aLTA and pLTA, and the involvement of aLTA in the pathogenesis of Staphylococcus aureus sepsis.
A unique cytoplasmic localization of retinoic acid receptor-gamma and its regulations.
Han, Young-Hoon,Zhou, Hu,Kim, Jin-Hee,Yan, Ting-dong,Lee, Kee-Ho,Wu, Hua,Lin, Feng,Lu, Na,Liu, Jie,Zeng, Jin-zhang,Zhang, Xiao-kun American Society for Biochemistry and Molecular Bi 2009 The Journal of biological chemistry Vol.284 No.27
<P>Recent evidence suggests that extranuclear action of retinoid receptors is involved in mediating the pleiotropic effects of retinoids. However, whether they reside in the cytoplasm remains elusive. Here, we showed that retinoic acid receptor-gamma (RARgamma) was cytoplasmic in confluent cells, or when cells were released from serum depletion or treated with growth factors. In studying the regulation of RARgamma subcellular localization, we observed that ectopically overexpressed RARgamma was mainly cytoplasmic irrespective of serum concentration and cell density. The cytoplasmic retention of RARgamma was inhibited by ligand retinoic acid (RA). In addition, coexpression of retinoid X receptor-alpha (RXRalpha) resulted in nuclear localization of RARgamma through their heterodimerization. Mutagenesis studies revealed that a C-terminal fragment of RXRalpha potently prevents RA-induced RARgamma nuclear localization and transcriptional function. Furthermore, our results showed that the cytoplasmic retention of RARgamma was due to the presence of its unique N-terminal A/B domain, which was subject to regulation by p38 MAPK-mediated phosphorylation. Deletion or mutation of the N-terminal A/B domain largely impaired its cytoplasmic localization. Together, our data demonstrate that the subcellular localization of RARgamma is regulated by complex interactions among ligand binding, receptor phosphorylation, and receptor dimerizations.</P>
Preparation of Spherical Bi3.25La0.75Ti3O12 Nanocrystals by a Sol-Gel Inverse Mini-Emulsion Approach
Han Longxiang,Zheng Lingjing,Hu Zhenxing,Yin Shilong,Zeng Yanwei 대한금속·재료학회 2014 ELECTRONIC MATERIALS LETTERS Vol.10 No.1
Monodisperse and spherical lanthanum bismuth titanate (Bi3.25La0.75Ti3O12, BLT) nanocrystals with an average size of ~60 nm were synthesized by in situ crystallization of spherical gel particles (~120 nm in diameter) at elevated temperatures in a carbonate environment. The gel particles were obtained by a sol-gel inverse mini-emulsion manipulation and a gelation process at 60°C. X-ray diffraction technique and Raman spectrum measurement were used to trace the formation of crystalline phases in the gel particles during heat treatment, and single-phase BLT nanocrystals were obtained after firing at 500°C for 4 h. The morphology and size of the gel particles and BLT nanocrystals were characterized by field-emission scanning electron microscopy and transmission electron microscopy. The possible mechanism underlying the formation of spherical BLT nanocrystals through a sol-gel inverse mini-emulsion approach was discussed.