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Shawn W. Gibson,Alix J. Conway,Zhifu Zheng,Tina M. Uchacz,Janet L. Taylor,Christopher D.Todd 한국식물학회 2012 Journal of Plant Biology Vol.55 No.5
In the plant cytoplasm signals from multiple sources interact to control development. Lateral root proliferation is controlled by the antagonistic action of the hormones auxin and abscisic acid (ABA). Auxin stimulates the separation of pericycle initials and cell expansion. ABA is required for lateral root initiation and elongation and acts as an antagonist through auxin-dependent pathways. These hormones also mediate reactive oxygen species (ROS)accumulation in growing roots and promote cell expansion. We describe the isolation and characterization of a copperand auxin-induced gene, COPPER INDUCED in LEAVES (CIL1), from Brassica carinata. Transgenic B. carinata seedlings expressing antisense CIL1 were used to determine the biological function of this gene. Lines with reduced CIL1expression showed a decrease in lateral root development, as well as reduced sensitivity to auxin and ABA. Steady-state analysis of redox components showed a decrease in NADPH oxidase, superoxide dismutase, and catalase activity,accompanied by an increase in hydrogen peroxide concentration. GFP-tagged CIL1 accumulated at the plasma membrane and in the apoplast indicating CIL1 is likely extracellular. From these data we propose that CIL1 is an extracellular protein involved in ROS cycling mediating auxin and ABA signals.
A Cu(1 1 1)(√3 X √3)R30° -Sb structure by impact collision ion-scattering spectroscopy
K.Umezawa,H.Takaoka,S.Hirayama,S.Nakanishi,W.M.Gibson 한국물리학회 2003 Current Applied Physics Vol.3 No.1
We have investigated the structures of Cu(111)(p3 .p3)R30.-Sb using time of ight-impact collision ion-scattering spec-troscopy. The experimental data and computer simulations support a structural model for the Cu(111)(p3 .p3)R30.-Sb structurein which Sb atoms displace up to 1=3 of the rst layer of Cu atoms and incorporate them into the rst Cu layer with the Sb atomsdisplaced outward 0.40.A with respect to the rst-layer Cu atoms. The outermost rst layer of Sb and Cu atoms shift from fcc- tohcp-hollow sites (only the top layer of Sb and Cu atoms occupies hcp hollow sites).. 2002 Elsevier Science B.V. All rights reserved.
Lee, S. H.,van der Werf, J. H. J.,Lee, S. H.,Park, E. W.,Oh, S. J.,Gibson, J. P.,Thompson, J. M. Blackwell Publishing Ltd 2010 Animal genetics Vol.41 No.4
<P>Summary</P><P>The objective of this study was to investigate an association between polymorphisms in the <I>FABP4</I> gene and phenotypic variation for marbling and carcass weight (CWT) in a population of Hanwoo steers. We re-sequenced 4.3 kb of the <I>FABP4</I> gene region in 24 Hanwoo bulls and identified 16 SNPs and 1 microsatellite polymorphism. Of these 16 SNPs, three SNPs [g.2774G>C (intron I), g.3473A>T (intron II) and g.3631G>A (exon III, creating a p.Met >Val amino acid substitution)] were genotyped in 583 steers to assess their association with carcass traits. The g.3473A allele showed a significant increasing effect on CWT (<I>P = </I>0.01) and the g.3631G allele was associated with higher marbling score (<I>P = </I>0.006). One haplotype of these three SNPs (CAG) was significantly associated with CWT (<I>P = </I>0.02) and marbling score (<I>P = </I>0.05) and could potentially be of value for marker assisted selection in Hanwoo cattle. The CAG haplotype effect for CWT was larger (11.14 ± 5.03 kg) than the largest single locus effect of g.3473A>T (5.01 ± 2.2 kg).</P>