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Enkhchimeg Vanjildorj,Tae‐Woong Bae,In‐Ja Song,Kyung‐Moon Kim,Yong‐Pyo Lim,Hyo‐Yeon Lee 한국육종학회 2008 한국육종학회지 Vol.40 No.2
Herbicide resistance is the most common trait being tested and thus herbicide‐resistant genetically modified plants are now the most widely cultivated worldwide. Here we developed herbicide‐resistant transgenic Agrostis mongolica Roshev. by employing an efficient Agrobacterium‐mediated transformation procedure with 25.2% of transformation efficiency. The identification and employment of regenerable and reproducible type of callus was one of the most critical factors to ensure success in this study. PCR analysis confirmed that the bar transgene was integrated into the genome of transgenic plants. The expression of 35S‐bar gene was confirmed by Northern blot analysis. The transgenic plants showed complete resistance to herbicide, indicating that the bar gene is functional in transgenic plants.
Environmental Risk Assessment of Genetically Engineered Herbicide-Tolerant <i>Zoysia japonica</i>
Bae, T. W.,Vanjildorj, E.,Song, S. Y.,Nishiguchi, S.,Yang, S. S.,Song, I. J.,Chandrasekhar, T.,Kang, T. W.,Kim, J. I.,Koh, Y. J.,Park, S. Y.,Lee, J.,Lee, Y.-E.,Ryu, K. H.,Riu, K. Z.,Song, P.-S.,Lee, H Wiley (John WileySons) 2008 Journal of environmental quality Vol.37 No.1
<P>Herbicide-tolerant Zoysia grass (Zoysia japonica Steud.) has been generated previously through Agrobacterium tumefaciens-mediated transformation. The genetically modified (GM) Zoysia grass survived Basta spraying and grew to maturity normally while the wild-type (WT) grass stopped growing and died. GM Zoysia grass will permit more efficient weed control for various turf grass plantings such as home lawns, golf courses, and parks. We examined the environmental/biodiversity risks of herbicide-tolerant GM Zoysia before applying to regulatory agencies for approval for commercial release. The GM and WT Zoysia grass' substantial trait equivalence, ability to cross-pollinate, and gene flow in confined and unconfined test fields were selectively analyzed for environmental/biodiversity effects. No difference between GM and WT Zoysia grass in substantial traits was found. To assess the potential for cross-pollination and gene flow, a non-selective herbicide, Basta, was used. Results showed that unintended cross-pollination with and gene flow from GM Zoysia grass were not detected in neighboring weed species examined, but were observed in WT Zoysia grass (on average, 6% at proximity, 1.2% at a distance of 0.5 m and 0.12% at a radius of 3 m, and 0% at distances over 3 m). On the basis of these initial studies, we conclude that the GM Zoysia grass generated in our laboratory and tested in the Nam Jeju County field does not appear to pose a significant risk when cultivated outside of test fields.</P>